BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,270,287	A→G	396 (TAA→TAG)	yhaC →	YhaC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,270,287	0	A	G	99.6%	786.8 / NA	234	396 (TAA→TAG)	yhaC	YhaC family protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (178/55); minor base T (1/0); total (179/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 234 total aligned reads displayed.

CTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTGATGCGCTACGC  >  NZ_CP025268/3270150‑3270422

cTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGAc                                                                                                                                       >  2:11470387/1‑140 (MQ=255)

cTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGAc                                                                                                                                       >  2:3848475/1‑140 (MQ=255)

  cGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACtt                                                                                                                                     >  2:1424991/1‑140 (MQ=255)

     taaaGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTtaat                                                                                                                                  <  1:6879272/139‑1 (MQ=255)

     gaAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTtaat                                                                                                                                  <  1:8887311/140‑1 (MQ=255)

      aaaGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTtaata                                                                                                                                 <  2:2478214/140‑1 (MQ=255)

        aGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAAc                                                                                                                               <  1:8494013/140‑1 (MQ=255)

         gACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAAct                                                                                                                              >  2:6323844/1‑140 (MQ=255)

         gACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAAct                                                                                                                              >  2:4826470/1‑140 (MQ=255)

                aTTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACa                                                                                                                       <  1:3848476/140‑1 (MQ=255)

                aTTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACa                                                                                                                       <  1:1836396/140‑1 (MQ=255)

                    tcCGGACAATGGCTTTGAGGTCGGGTAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGt                                                                                                                   <  1:10109357/140‑1 (MQ=255)

                      cGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCt                                                                                                                 <  1:4558820/140‑1 (MQ=255)

                      cGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCt                                                                                                                 <  2:5463405/140‑1 (MQ=255)

                           aaTGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCtattat                                                                                                            <  2:4729015/140‑1 (MQ=255)

                             tGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTTCGACCTTTTAAATGCAATATATACTTAATAACTTCTTACAAAGTCTAttatta                                                                                                          >  2:6565584/1‑140 (MQ=255)

                                       gTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGa                                                                                                >  2:5862368/1‑140 (MQ=255)

                                              aaTATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAtatttat                                                                                         <  1:2526357/140‑1 (MQ=255)

                                              aaTATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAtatttat                                                                                         <  1:5206817/140‑1 (MQ=255)

                                               atatAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAgagttagt                                                                                        >  2:10940544/1‑138 (MQ=255)

                                                        atatGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAATTGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTAcc                                                                               >  2:7608856/1‑140 (MQ=255)

                                                        atatGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTtaat                                                                                                                                  >  1:1637120/1‑89 (MQ=255)

                                                        atatGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTtaat                                                                                                                                  <  2:1637120/89‑1 (MQ=255)

                                                        atatGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACa                                                                                                                       <  2:4360730/100‑1 (MQ=255)

                                                        atatGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACa                                                                                                                       >  1:4360731/1‑100 (MQ=255)

                                                        atatGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTAcc                                                                               >  2:3356381/1‑140 (MQ=255)

                                                        atatGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTAcc                                                                               <  1:5862369/140‑1 (MQ=255)

                                                          atGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCt                                                                             >  1:6111784/1‑140 (MQ=255)

                                                          atGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCt                                                                             >  1:9408187/1‑140 (MQ=255)

                                                          atGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCt                                                                             >  2:4967430/1‑140 (MQ=255)

                                                          atGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCt                                                                             >  2:7934113/1‑140 (MQ=255)

                                                          atGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATGTATTCATCTTACCCt                                                                             >  1:7945377/1‑140 (MQ=255)

                                                                  tataACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCa                                                                     >  2:985007/1‑140 (MQ=255)

                                                                  tataACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCa                                                                     >  1:4384731/1‑140 (MQ=255)

                                                                    taACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCaaa                                                                   >  2:4188353/1‑140 (MQ=255)

                                                                    taACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCaaa                                                                   >  2:5247802/1‑140 (MQ=255)

                                                                       cTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGc                                                                >  2:3080768/1‑140 (MQ=255)

                                                                       cTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGc                                                                >  2:532875/1‑140 (MQ=255)

                                                                          agagAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCaaa                                                             >  1:776555/1‑140 (MQ=255)

                                                                          agagAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCaaa                                                             >  2:768311/1‑140 (MQ=255)

                                                                          agagAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCaaa                                                             >  1:7110811/1‑140 (MQ=255)

                                                                            agaAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCt                                                                                                                 <  1:11207811/86‑1 (MQ=255)

                                                                            agaAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGat                                                                                               >  2:7195102/1‑104 (MQ=255)

                                                                            agaAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCaaaaa                                                           >  2:11181777/1‑140 (MQ=255)

                                                                            agaAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATGCATCTTACCCTGTGCACCAAAAGCaaaaa                                                           >  1:9916782/1‑140 (MQ=255)

                                                                             gaAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTtaata                                                                                                                                 <  2:6792847/69‑1 (MQ=255)

                                                                             gaAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTtaata                                                                                                                                 >  1:6792848/1‑69 (MQ=255)

                                                                                  aaTATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACt                                                     >  2:10629289/1‑140 (MQ=255)

                                                                                       tttACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGAcgc                                                <  2:8853670/140‑1 (MQ=255)

                                                                                         tACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCgg                                              >  2:11535209/1‑140 (MQ=255)

                                                                                           ccAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGt                                            >  2:10811148/1‑140 (MQ=255)

                                                                                              gagagGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACACTGTGCACCAAAAGCAAAAACTCACTGACTCGGGTTTg                                         >  2:694700/1‑140 (MQ=255)

                                                                                                gagGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGaa                                       >  2:8934786/1‑140 (MQ=255)

                                                                                                gagGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGaa                                       >  1:8313389/1‑140 (MQ=255)

                                                                                                gagGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGaa                                       >  1:3231595/1‑140 (MQ=255)

                                                                                                   gACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACt                                    >  2:1512457/1‑140 (MQ=255)

                                                                                                   gACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACACTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACt                                    >  2:3284637/1‑140 (MQ=255)

                                                                                                       agaCGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATg                                <  2:87133/138‑1 (MQ=255)

                                                                                                        taCGTATGATTACGACCTTTTAAATGCAATATAGAc                                                                                                                                       <  1:7195900/36‑1 (MQ=255)

                                                                                                        taCGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATgg                               >  1:2740785/1‑140 (MQ=255)

                                                                                                             aTGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAATGTCTATTATTAATACCGTAGATATTTATTCTTCTTACCCTGTGCACCAAAAGCAAAAACTCACTGAGGCGGGTTTGAAACTGATGGCTAcg                          >  2:1613344/1‑139 (MQ=255)

                                                                                                             aTGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATTCCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGCTGGCAAcc                          >  2:6994969/1‑140 (MQ=255)

                                                                                                             aTGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATCCCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAACCTGATGGCAAcc                          >  2:8437250/1‑140 (MQ=255)

                                                                                                             aTGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAAcc                          >  2:4386225/1‑140 (MQ=255)

                                                                                                             aTGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAAcc                          >  2:1904255/1‑140 (MQ=255)

                                                                                                             aTGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAAcc                          >  1:1015684/1‑140 (MQ=255)

                                                                                                             aTGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAAcc                          >  2:9717425/1‑140 (MQ=255)

                                                                                                             aTGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAAcc                          >  1:3248574/1‑140 (MQ=255)

                                                                                                             aTGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAAcc                          >  1:4108580/1‑140 (MQ=255)

                                                                                                             aTGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACACTGTGCACCAAAATCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAAcc                          >  1:140500/1‑140 (MQ=255)

                                                                                                              tGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTATATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCg                         >  2:5696797/1‑140 (MQ=255)

                                                                                                              tGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCg                         >  2:11165163/1‑140 (MQ=255)

                                                                                                              tGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCg                         >  1:2575446/1‑140 (MQ=255)

                                                                                                                aTTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAtattta                                                                                          >  2:7518781/1‑73 (MQ=255)

                                                                                                                aTTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAtattta                                                                                          <  1:7518782/73‑1 (MQ=255)

                                                                                                                aTTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCa                       >  2:2923541/1‑140 (MQ=255)

                                                                                                                aTTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCa                       >  1:10544017/1‑140 (MQ=255)

                                                                                                                aTTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCa                       >  1:964705/1‑140 (MQ=255)

                                                                                                                aTTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCa                       >  2:2579377/1‑140 (MQ=255)

                                                                                                                aTTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCa                       >  1:7846805/1‑140 (MQ=255)

                                                                                                                aTTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCAATTACGCGGGTTTGAAACAGATGGCAACCGCa                       >  2:2568878/1‑140 (MQ=255)

                                                                                                                aTTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCATAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCa                       >  2:10175321/1‑140 (MQ=255)

                                                                                                                aTTACGACCTGTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCGGTGCCCCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCa                       >  1:4172600/1‑140 (MQ=255)

                                                                                                                  tACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCaaa                     >  2:9333900/1‑140 (MQ=255)

                                                                                                                  tACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCaaa                     <  2:3243415/140‑1 (MQ=255)

                                                                                                                  tACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCaaa                     >  2:6137737/1‑140 (MQ=255)

                                                                                                                  tACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCaaa                     >  1:2322570/1‑140 (MQ=255)

                                                                                                                  tACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCaaa                     >  2:1323683/1‑140 (MQ=255)

                                                                                                                  tACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCaaa                     >  2:11336698/1‑140 (MQ=255)

                                                                                                                    cGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCaaaat                   >  1:7645564/1‑139 (MQ=255)

                                                                                                                      aCCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATg                 >  1:1868294/1‑140 (MQ=255)

                                                                                                                          tttAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTg             <  1:1348458/140‑1 (MQ=255)

                                                                                                                          tttAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTg             >  2:1247769/1‑140 (MQ=255)

                                                                                                                          tttAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTg             >  2:1029880/1‑140 (MQ=255)

                                                                                                                          tttAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTg             >  1:9951875/1‑140 (MQ=255)

                                                                                                                             aaaTGCAATATAGACTTAATAACTTCTTAAAAAGTCTATTATTAATACCGTAGATATTTATTCATCTGACCCTGTGCACCAAAAGCAAATACTCCCTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAAGGCCTGATg          <  2:2769532/140‑1 (MQ=255)

                                                                                                                                  cAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTGGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTGATGCGCTa     >  2:3598062/1‑140 (MQ=255)

                                                                                                                                     tataGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTGATGCGCTACGc  >  1:2941861/1‑140 (MQ=255)

                                                                                                                                     tataGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTGATGCGCTACGc  >  1:10084323/1‑140 (MQ=255)

                                                                                                                                     tataGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTAATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTGATGCGCTACGc  >  1:10777932/1‑140 (MQ=255)

CTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAAACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTGATGCGCTACGC  >  NZ_CP025268/3270150‑3270422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`