Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,516,412 C→T C63Y (TGT→TAT)  dam ← adenine‑specific DNA‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,516,4120CT99.3% 2054.2 / NA 548C63Y (TGT→TAT) damadenine‑specific DNA‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (300/243);  minor base G (1/3);  total (302/246)
Fisher's exact test for biased strand distribution p-value = 3.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 548 total aligned reads displayed.

CGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAG  >  NZ_CP025268/3516276‑3516548
                                                                                                                                        |                                                                                                                                        
cGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATaga                                                                                                                                       <  2:7770563/140‑1 (MQ=255)
 gCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATagag                                                                                                                                      <  1:2622045/140‑1 (MQ=255)
  cccGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAgaga                                                                                                                                     >  1:1303033/1‑140 (MQ=255)
  cccGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAgaga                                                                                                                                     >  2:1259703/1‑140 (MQ=255)
       cggaacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCCCAATGTTATAGAGACTGAt                                                                                                                                <  2:5401365/140‑1 (MQ=255)
       cggaacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGAt                                                                                                                                <  2:3178306/140‑1 (MQ=255)
       cggaacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGAt                                                                                                                                >  1:8132674/1‑140 (MQ=255)
       cggaacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGAt                                                                                                                                <  2:6150998/140‑1 (MQ=255)
       cggaacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGAt                                                                                                                                <  2:8261018/140‑1 (MQ=255)
         gaacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAATCAGCTCGCGTTCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCa                                                                                                                              <  1:9442464/140‑1 (MQ=255)
            cggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGt                                                                                                                           <  2:2529513/140‑1 (MQ=255)
            cggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGt                                                                                                                           >  1:3059434/1‑140 (MQ=255)
            cggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACGGATCAGGt                                                                                                                           <  2:11355287/140‑1 (MQ=255)
             ggctCCTGGCTTTTGTTGAACTCTTCGCGGAACTGAGAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTc                                                                                                                          <  2:6911267/137‑1 (MQ=255)
             ggaTCCTGGCTTTTGTTGAACTCTTCGCTGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGACTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTc                                                                                                                          <  2:7254986/140‑1 (MQ=255)
             ggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTc                                                                                                                          <  2:3948933/140‑1 (MQ=255)
              gaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCg                                                                                                                         <  1:5398323/140‑1 (MQ=255)
                 ccTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTa                                                                                                                      >  1:9673078/1‑140 (MQ=255)
                    ggCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGNACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTg                                                                                                                   >  1:6294929/1‑140 (MQ=255)
                      cTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGat                                                                                                                 <  1:8376531/140‑1 (MQ=255)
                       ttttGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGata                                                                                                                <  2:8778433/140‑1 (MQ=255)
                          agttgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTAATAGAGACTGATCAGGTCGCTAATGATATCg                                                                                                             >  1:1623574/2‑140 (MQ=255)
                            ttgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGc                                                                                                           <  1:282042/140‑1 (MQ=255)
                            ttgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTCATAGAGACTGATCAGGTCGCTATTGATATCGGc                                                                                                           <  2:1845554/140‑1 (MQ=255)
                             tgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCa                                                                                                          <  1:3253252/140‑1 (MQ=255)
                                     tCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGAtata                                                                                                  <  1:4736476/140‑1 (MQ=255)
                                      cgcgGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATaa                                                                                                 <  2:6703397/140‑1 (MQ=255)
                                        cgGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACg                                                                                               <  2:7493775/140‑1 (MQ=255)
                                           aaCTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACgaga                                                                                            >  2:10091761/1‑140 (MQ=255)
                                                agaGTACACCTCGGCGCAATTTGCGTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCGTCACACTGTTATCGAGACGGATCAGGGCGCTATTGATCTCGGCAAGGATATACCGAGAAAAGt                                                                                       <  2:11254635/138‑1 (MQ=255)
                                                aTAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGt                                                                                       >  1:6608884/1‑140 (MQ=255)
                                                aTAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGt                                                                                       >  2:10838926/1‑140 (MQ=255)
                                                aTAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGt                                                                                       >  1:6418364/1‑140 (MQ=255)
                                                   gTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCgg                                                                                    <  1:2413307/140‑1 (MQ=255)
                                                   gTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCgg                                                                                    <  2:2717509/140‑1 (MQ=255)
                                                     aaaCCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGAGCGGCCTGTACGTCCTCATCAGTACGCATCTTCACAATGTTAGAGAGACAGATCAGCTCGCTATTGCTATCGGCAAGGATATAACGAGaaaacgcgggg                                                                                  <  2:5988767/140‑8 (MQ=255)
                                                     aaaCCTCGGCGCAATTTGGTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGgtg                                                                                  <  2:8584479/140‑1 (MQ=255)
                                                     aaaCCTCGGCGCAATGTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGgtg                                                                                  <  2:9136146/140‑1 (MQ=255)
                                                       aCCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGtt                                                                                >  1:29745/1‑140 (MQ=255)
                                                        ccTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTg                                                                               >  2:962355/1‑140 (MQ=255)
                                                           cGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTgaga                                                                            >  1:6163543/1‑140 (MQ=255)
                                                           cGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTgaga                                                                            >  1:4123119/1‑140 (MQ=255)
                                                              cgcAATTTGTTTCGGGAACAAACCGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGacac                                                                         >  1:6456021/1‑140 (MQ=255)
                                                              cgcAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAac                                                                         >  1:7775970/1‑140 (MQ=255)
                                                                cAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAacac                                                                       >  1:1867497/1‑140 (MQ=255)
                                                                cAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGAATCTTCACAATGTTATAGAGAATGATCAGGTCGCTATTGATATCGGCAAGTATATAACGATAAAAGTCGGTGTTGAGAAacac                                                                       <  1:9557279/140‑1 (MQ=255)
                                                                        ttCGGTAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATATTCAAAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCgg                                                               <  1:9026052/140‑1 (MQ=255)
                                                                            ggAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGTTGTTGAGAAACACCGACCCGGCAcc                                                           <  2:1568856/140‑1 (MQ=255)
                                                                                     aGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGAAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAgg                                                  <  1:827904/140‑1 (MQ=255)
                                                                                       cTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCt                                                >  2:4150370/1‑140 (MQ=255)
                                                                                       cTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCt                                                >  1:9957113/1‑140 (MQ=255)
                                                                                           cgTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAAc                                            >  2:3625048/1‑140 (MQ=255)
                                                                                            gTGCGGCCTGTACTTACTCATCAGTACGCATATTCACAATGTTATAGTGCCTTCTCCGGTCGATATTGATATCGGCACGGATATAACGAGAAAAGTCGGTGTTTAGAAACACCGACCCGGCACCTCCAAAAGGCTCAAcc                                           <  1:392132/140‑1 (MQ=255)
                                                                                                 gCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACa                                      >  2:4394103/1‑140 (MQ=255)
                                                                                                    tGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTc                                   <  2:4733479/140‑1 (MQ=255)
                                                                                                            cTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTgg                           >  2:3293058/1‑140 (MQ=255)
                                                                                                            cTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTgg                           >  1:10990989/1‑140 (MQ=255)
                                                                                                            cTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTgg                           >  1:9263020/1‑140 (MQ=255)
                                                                                                            cTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTgg                           >  1:204201/1‑140 (MQ=255)
                                                                                                            cTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTgg                           >  2:11615857/1‑140 (MQ=255)
                                                                                                            cTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTAGCCCGTgg                           >  1:5951127/1‑140 (MQ=255)
                                                                                                            cTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGGCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTgg                           >  2:4073391/1‑140 (MQ=255)
                                                                                                              catcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGc                         >  1:9100027/1‑140 (MQ=255)
                                                                                                              catcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGc                         >  1:11260448/1‑140 (MQ=255)
                                                                                                              catcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGc                         >  1:11579685/1‑140 (MQ=255)
                                                                                                                tccgTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCACGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCaa                       >  2:7840001/4‑140 (MQ=255)
                                                                                                                tcaGTACGCATCTTCACAATGTTATAGAGTCTGATCAGGTCGCTATTGTTATCGGCAAGGATATAACGAGAAAAGTCGGTGGTGTGAAACACCGACCCGGCACCTACAAAAGGCTCCACCAGACATTCGCGCTTGGGCaa                       >  1:5257010/1‑140 (MQ=255)
                                                                                                                tcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACTAGAAAAGTCGGTTATGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTAGCCCTAGGTCaa                       >  2:4827389/1‑140 (MQ=255)
                                                                                                                tcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCgg                                                                                    <  1:5775525/79‑1 (MQ=255)
                                                                                                                tcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCaa                       >  1:3399102/1‑140 (MQ=255)
                                                                                                                tcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCaa                       >  1:5011060/1‑140 (MQ=255)
                                                                                                                tcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCaa                       >  1:8765603/1‑140 (MQ=255)
                                                                                                                tcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACAACGACCCGGCCCCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCaa                       >  1:4591519/1‑140 (MQ=255)
                                                                                                                tcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGGTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCaa                       >  1:5583452/1‑140 (MQ=255)
                                                                                                                tcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGGGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCCGACATTCGCCCGTGGGCaa                       >  2:631926/1‑140 (MQ=255)
                                                                                                                tcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTAGTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCaa                       >  2:2247062/1‑140 (MQ=255)
                                                                                                                  aGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAAt                     >  2:10616179/1‑140 (MQ=255)
                                                                                                                   ttACGCATCTTCAAAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATg                    <  2:1028553/139‑1 (MQ=255)
                                                                                                                   gTACGCATCTTCACAATGTTATAGAGACTGCTCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTTAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATg                    <  2:9680449/140‑1 (MQ=255)
                                                                                                                   gTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATg                    <  1:1755622/140‑1 (MQ=255)
                                                                                                                    tACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGc                   >  1:11518170/1‑140 (MQ=255)
                                                                                                                      cGCATCTTCACAATGTTATAGAGACTGATCAGGGCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGAGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCg                 <  2:5813255/140‑1 (MQ=255)
                                                                                                                         ctCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGttt              >  2:2888596/2‑140 (MQ=255)
                                                                                                                         aTCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGttt              >  2:762886/1‑140 (MQ=255)
                                                                                                                           cTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTaa            >  1:8535189/1‑140 (MQ=255)
                                                                                                                           cTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTaa            >  1:7963068/1‑140 (MQ=255)
                                                                                                                           cTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTaa            <  1:6962046/140‑1 (MQ=255)
                                                                                                                              cacaATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAatat         >  1:9829525/1‑140 (MQ=255)
                                                                                                                              cacaATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAatat         >  1:1449767/1‑140 (MQ=255)
                                                                                                                              cacaATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAatag         >  2:10539503/1‑139 (MQ=255)
                                                                                                                                caATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGTATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATatca       <  1:4418663/140‑1 (MQ=255)
                                                                                                                                caATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATatca       <  1:1045171/140‑1 (MQ=255)
                                                                                                                                caATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATatca       <  1:4963380/140‑1 (MQ=255)
                                                                                                                                 aaTGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATatcat      >  1:10486402/1‑140 (MQ=255)
                                                                                                                                  aTGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCGCTTGGGCAAATGCCGTTTAATatcatc     <  2:6503754/140‑1 (MQ=255)
                                                                                                                                  aTGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATatcatc     >  1:8860761/1‑140 (MQ=255)
                                                                                                                                   tGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATAtcatca    >  1:10238107/1‑140 (MQ=255)
                                                                                                                                     ttATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGt                                                                                       <  1:7400000/55‑1 (MQ=255)
                                                                                                                                     ttATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAg  >  2:11047486/1‑140 (MQ=255)
                                                                                                                                     ttATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATAAAg  >  1:3555960/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                        
CGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTACAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAG  >  NZ_CP025268/3516276‑3516548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑