BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,567,287	T→C	478 (TAA→TAG)	glgA ←	glycogen synthase GlgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,567,287	0	T	C	99.5%	2241.9 / NA	647	478 (TAA→TAG)	glgA	glycogen synthase GlgA
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (341/302); minor base A (2/1); total (344/303)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 652 total aligned reads displayed.

TCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAATTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGAATAGCCC  >  NZ_CP025268/3567150‑3567423

   tGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTAttt                                                                                                                                     <  2:11157131/140‑1 (MQ=255)

       tATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAg                                                                                                                                 <  1:7895892/140‑1 (MQ=255)

          tGGCGTCGACCGTGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGAGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGa                                                                                                                              <  2:10431609/140‑1 (MQ=255)

          tGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGa                                                                                                                              <  1:8069445/140‑1 (MQ=255)

          tGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGa                                                                                                                              <  1:4640258/140‑1 (MQ=255)

           ggCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGAt                                                                                                                             >  1:9663580/1‑140 (MQ=255)

            gCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATa                                                                                                                            >  1:6339344/1‑140 (MQ=255)

              gacgacCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGt                                                                                                                          <  1:4067267/140‑1 (MQ=255)

              gacgacCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGt                                                                                                                          >  1:7174666/1‑140 (MQ=255)

               acgacCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGCGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTa                                                                                                                         >  2:7573312/1‑140 (MQ=255)

               acgacCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTa                                                                                                                         <  1:1392650/140‑1 (MQ=255)

               acgacCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTa                                                                                                                         >  2:8404299/1‑140 (MQ=255)

               acgacCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTa                                                                                                                         >  1:621600/1‑140 (MQ=255)

                    cGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCt                                                                                                                    <  2:4556378/140‑1 (MQ=255)

                    cGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCt                                                                                                                    <  2:794204/140‑1 (MQ=255)

                    cGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCt                                                                                                                    <  2:5461816/140‑1 (MQ=255)

                        tCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGCATATGTAAACGGAGCAGTCTTATAGGCGTTTCCTGAAAACGATTTCAAGCGATAGTAAAGCTCACg                                                                                                                >  2:6420601/1‑140 (MQ=255)

                            ttCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTAc                                                                                                            >  2:1244731/1‑140 (MQ=255)

                               cAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGGTAGTAAAGCTCACGGTACGAc                                                                                                         <  2:3867242/140‑1 (MQ=255)

                                 aTCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACtt                                                                                                       >  1:742087/1‑140 (MQ=255)

                                 aTCGTAAACAACAGCTTGTAAGCGAAAGACTGCATAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTGCCTGAAAACTATTTCAATCGATAGTAAAGAGCACGGTACGACtt                                                                                                       >  2:8908859/1‑140 (MQ=255)

                                     tAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTCTTTCAAGCGATAGTAAAGCTCACGGTACGACTTcgcc                                                                                                   >  2:6804854/1‑140 (MQ=255)

                                     tAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTcgcc                                                                                                   >  1:3505550/1‑140 (MQ=255)

                                          aTCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGAc                                                                                              <  1:2016369/140‑1 (MQ=255)

                                            cAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCt                                                                                            >  2:7729618/1‑140 (MQ=255)

                                            cAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGATCACGGTACGACTTCGCCGCGACCt                                                                                            >  2:11522324/1‑140 (MQ=255)

                                              gCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGc                                                                                          <  1:11308644/140‑1 (MQ=255)

                                               cTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGcc                                                                                         <  1:2966602/140‑1 (MQ=255)

                                                    aaGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTa                                                                                    <  1:6652819/140‑1 (MQ=255)

                                                         aTAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATc                                                                               >  1:10215123/1‑140 (MQ=255)

                                                           agagTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCa                                                                             <  1:4235273/140‑1 (MQ=255)

                                                           agagTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCa                                                                             <  2:6273699/140‑1 (MQ=255)

                                                           agagTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCa                                                                             <  1:7665563/140‑1 (MQ=255)

                                                           agagTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCa                                                                             <  1:10613471/140‑1 (MQ=255)

                                                           agagTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCa                                                                             <  1:1756290/140‑1 (MQ=255)

                                                                 cTTCAGAGCTTCTACGCTACGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAATCTATTTCTAGCGATAGTAAAGCTCTCGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATGGCCa                                                                       <  2:6989268/140‑1 (MQ=255)

                                                                 cTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCa                                                                       >  1:10457896/1‑140 (MQ=255)

                                                                 cTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCa                                                                       <  2:5700222/140‑1 (MQ=255)

                                                                 cTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCa                                                                       <  1:3172571/140‑1 (MQ=255)

                                                                 cTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCa                                                                       >  1:10945419/1‑140 (MQ=255)

                                                                 cTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCa                                                                       >  1:8945934/1‑140 (MQ=255)

                                                                 cTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCa                                                                       >  1:8765807/1‑140 (MQ=255)

                                                                   gaaGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATa                                                                     >  2:3197779/2‑140 (MQ=255)

                                                                    aaGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAg                                                                    >  2:5278866/1‑140 (MQ=255)

                                                                      gagCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGcc                                                                  >  2:8471455/1‑140 (MQ=255)

                                                                      gagCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGcc                                                                  <  2:2934455/140‑1 (MQ=255)

                                                                      gagCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGcc                                                                  <  2:4204835/140‑1 (MQ=255)

                                                                      gagCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCAGAGAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGcc                                                                  <  1:4924032/140‑1 (MQ=255)

                                                                         cTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGa                                                               >  2:9807004/1‑140 (MQ=255)

                                                                           tCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACg                                                             >  1:6936316/1‑140 (MQ=255)

                                                                            cTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTGCCTGAAAACTCTTTCAAGCGATAGTAAAGCTCACGGTACGACGTCGCCGCGACCAGCCAGCTCAAATCCATTGCCATAGCCTGACGt                                                            >  2:1754857/1‑140 (MQ=255)

                                                                            cTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCAATCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGt                                                            >  2:5907541/1‑140 (MQ=255)

                                                                                gCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGca                                                        <  2:7142044/140‑1 (MQ=255)

                                                                                    aGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACaaa                                                    >  1:3824101/1‑140 (MQ=255)

                                                                                    aGCGTGGGCGATGAATATGGAAACGGAGAATTCATATAGGCGTTTGGTGCAAACTCTTTGCTGCGAGAGTACAGCTCACGGTGCGTCTTCGCCGCGGCCTGCCCGCTCAGGGGCATTGCCATAGGCTGAGGTTGGACaaa                                                    >  2:2349827/1‑140 (MQ=255)

                                                                                      cGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAAcc                                                  >  1:1153249/1‑140 (MQ=255)

                                                                                         gggCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGcc                                               <  2:2255148/140‑1 (MQ=255)

                                                                                          ggCGATGAATTTGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCca                                              <  2:158372/140‑1 (MQ=255)

                                                                                          ggCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCca                                              <  1:11002503/140‑1 (MQ=255)

                                                                                          ggCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCca                                              <  1:2795838/140‑1 (MQ=255)

                                                                                          ggCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCca                                              <  1:9051006/140‑1 (MQ=255)

                                                                                          ggCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCca                                              >  1:5867907/1‑140 (MQ=255)

                                                                                              aTGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGt                                          >  1:8430472/1‑140 (MQ=255)

                                                                                              aTGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGt                                          >  1:8303778/1‑140 (MQ=255)

                                                                                              aTGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGt                                          >  2:9402976/1‑140 (MQ=255)

                                                                                                    atGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGa                                    <  2:6619827/140‑1 (MQ=255)

                                                                                                     tGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTCCGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCTCAAACCGCCACAGTGAAGGAc                                   >  1:2347475/1‑140 (MQ=255)

                                                                                                     tGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGAc                                   <  1:10046874/140‑1 (MQ=255)

                                                                                                     tGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGAc                                   >  1:9904857/1‑140 (MQ=255)

                                                                                                          aCGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGAc                              <  1:2201172/140‑1 (MQ=255)

                                                                                                          aCGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGAc                              <  2:9025171/140‑1 (MQ=255)

                                                                                                          aCGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGAc                              <  2:10945418/140‑1 (MQ=255)

                                                                                                          aCGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGAc                              <  2:601703/140‑1 (MQ=255)

                                                                                                          aCGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGAc                              <  1:5449333/140‑1 (MQ=255)

                                                                                                          aCGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGACACAGTGAAGGACGGGAc                              <  1:2575923/140‑1 (MQ=255)

                                                                                                           cGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGAcc                             >  1:858128/1‑140 (MQ=255)

                                                                                                           cGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGAcc                             >  1:5170859/1‑140 (MQ=255)

                                                                                                            ggAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGTCCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACca                            <  2:10068348/140‑1 (MQ=255)

                                                                                                             gAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTTCACAAACCGCCACAGTGAAGGACGGGACcac                           >  1:9142278/1‑140 (MQ=255)

                                                                                                             gAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACcac                           >  2:10263438/1‑140 (MQ=255)

                                                                                                              aGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACcaca                          >  2:2630334/1‑140 (MQ=255)

                                                                                                              aGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACcaca                          >  2:3040224/1‑140 (MQ=255)

                                                                                                              aGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACcaca                          >  1:6418618/1‑140 (MQ=255)

                                                                                                              aGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACcaca                          >  2:3696724/1‑140 (MQ=255)

                                                                                                              aGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACcaca                          >  2:3530741/1‑140 (MQ=255)

                                                                                                                cATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGt                        >  2:4807833/1‑140 (MQ=255)

                                                                                                                      tataGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACaaaa                  <  2:1932108/140‑1 (MQ=255)

                                                                                                                        taGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGc                <  1:1847237/140‑1 (MQ=255)

                                                                                                                          ggCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCAc              >  2:3275531/1‑140 (MQ=255)

                                                                                                                             gTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTc           >  2:9193989/1‑140 (MQ=255)

                                                                                                                               ttCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGa         >  2:6165874/1‑140 (MQ=255)

                                                                                                                               ttCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGa         >  2:7287298/1‑140 (MQ=255)

                                                                                                                                tCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGc                                                                                          <  1:5958667/58‑1 (MQ=255)

                                                                                                                                tCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTACACTCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGaa        <  2:5160406/140‑1 (MQ=255)

                                                                                                                                tCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGaa        >  1:3065441/1‑140 (MQ=255)

                                                                                                                                tCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGaa        <  2:10628462/140‑1 (MQ=255)

                                                                                                                                tCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGaa        <  1:5599361/140‑1 (MQ=255)

                                                                                                                                  cTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGAATa      <  1:956034/140‑1 (MQ=255)

                                                                                                                                     aaaaCTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGAATAGcc   >  1:1539480/1‑140 (MQ=255)

                                                                                                                                     aaaaCTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGAATAGcc   >  2:2691915/1‑140 (MQ=255)

TCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAATTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGAATAGCCC  >  NZ_CP025268/3567150‑3567423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`