BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	353,643	A→G	629 (TAA→TAG)	prpE →	propionate‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	353,643	0	A	G	99.5%	2262.7 / NA	651	629 (TAA→TAG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (225/422); minor base T (1/2); total (226/425)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 651 total aligned reads displayed.

AATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCT  >  NZ_CP025268/353507‑353779

   tGCCAAAAACGCGATCCGGAAAAAGGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGAGCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTAtt                                                                                                                                    <  2:8414542/140‑1 (MQ=255)

                aTCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTcg                                                                                                                       <  2:3411612/140‑1 (MQ=255)

                  ccGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTcgcg                                                                                                                     <  2:9204831/140‑1 (MQ=255)

                  ccGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGNCGGATGCGTcgcg                                                                                                                     <  1:10007548/140‑1 (MQ=255)

                  ccGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCAGTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATGGTCGGATGCGTcgcg                                                                                                                     <  2:295145/140‑1 (MQ=255)

                    ggAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTcgcgcg                                                                                                                   <  2:4942760/140‑1 (MQ=255)

                    ggAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCAGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTAGTGTCGGATGCGTcgcgcg                                                                                                                   <  1:936916/140‑1 (MQ=255)

                      acacaTGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATAGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGt                                                                                                                 <  1:9947970/136‑1 (MQ=255)

                      aaaaaTGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCTGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGt                                                                                                                 <  1:9524844/140‑1 (MQ=255)

                      aaaaaTGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGt                                                                                                                 <  2:1410795/140‑1 (MQ=255)

                      aaaaaTGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGt                                                                                                                 <  1:7912012/140‑1 (MQ=255)

                      aaaaaTGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGt                                                                                                                 <  1:1246047/140‑1 (MQ=255)

                      aaaaaTGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGt                                                                                                                 <  1:1899199/140‑1 (MQ=255)

                      aaaaaTGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGt                                                                                                                 <  1:3025637/140‑1 (MQ=255)

                      aaaaaTGCAGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGACGAGTAGGTTATTGTCGGATGCGTCGCGCGGt                                                                                                                 <  1:2740305/140‑1 (MQ=255)

                       aaaaTGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTg                                                                                                                >  2:3386996/1‑140 (MQ=255)

                        caacgctgcGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGAACTGACGACCATCGATGATCCTCCGACGATCGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATGGTCGGATGCGACGCGCGGTGc                                                                                                               <  2:5062604/136‑1 (MQ=255)

                        aaaTGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGc                                                                                                               <  2:9385632/140‑1 (MQ=255)

                          aTGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTATCGGATGCGTCGCGCGGTGCAt                                                                                                             <  1:7773334/140‑1 (MQ=255)

                           tgctgcGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATc                                                                                                            >  2:2390581/1‑140 (MQ=255)

                           tgctgcGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGGTATTGTCGGATGCGTCGCGCGGTGCATc                                                                                                            >  1:2156948/1‑140 (MQ=255)

                             ctgcGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCg                                                                                                          <  1:6044902/140‑1 (MQ=255)

                                cgccgcCCGAGCCAGGCGATATGTGACGGACGCGTTCCTGGAGATCTGACGACCATTGATGATCCTGCGGCGTTTGTTCAGAGCCGCCAGGCGATGGAAGAGTAGGTTATTCGCGTCTGCGTCGCGCGGTGCATCCGGCa                                                                                                       <  2:4042868/140‑1 (MQ=255)

                                cgccgcACGCTCCAGGCGAATTGCGCAGGACGCGAGCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCATATCCGCCAGGCGATGGAAGAGTAGGTTCTTGTCGGATTCGTCGCGCGGTGCATCCGGCa                                                                                                       <  2:7721615/140‑1 (MQ=255)

                                cgccgcACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                       <  1:1420947/140‑1 (MQ=255)

                                cgccgcACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                       <  2:9767544/140‑1 (MQ=255)

                                cgccgcACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                       <  2:10049865/140‑1 (MQ=255)

                                cgccgcACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                       <  1:11176824/140‑1 (MQ=255)

                                cgccgcACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                       >  2:6203866/1‑140 (MQ=255)

                                cgccgcACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                       <  1:22843/140‑1 (MQ=255)

                                cgccgcACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                       <  2:5230545/140‑1 (MQ=255)

                                cgccgcACGATCCAGGCGAGTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                       <  2:6919590/140‑1 (MQ=255)

                                     cACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACtgtg                                                                                                  >  1:8894964/1‑140 (MQ=255)

                                     cACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGAGGGAAGAGTAGGTGATTGTCGGATGCGTCGCGCGGTGCATCCGGCACtgtg                                                                                                  >  2:10869910/1‑140 (MQ=255)

                                                    ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                   >  1:8462017/1‑140 (MQ=255)

                                                    ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                   >  2:1734535/1‑140 (MQ=255)

                                                    ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                   >  1:3903556/1‑140 (MQ=255)

                                                    ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                   >  2:4181679/1‑140 (MQ=255)

                                                    ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                   >  1:3532813/1‑140 (MQ=255)

                                                    ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                   >  2:8936830/1‑140 (MQ=255)

                                                    ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                   >  2:904271/1‑140 (MQ=255)

                                                    ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGACGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                   >  1:9718949/1‑140 (MQ=255)

                                                             aCGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGa                                                                          >  1:8142034/1‑140 (MQ=255)

                                                             aCGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGa                                                                          <  2:471955/140‑1 (MQ=255)

                                                             aCGCGATCCTGGAGATCTGACGACAATTGATGATCCTGCGTGGATGGATCTGATCAGCCTGTCTATGGAAGTGTAGGTTATTGTCGGTTGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATTCCTGATGCGACGCTGa                                                                          <  2:5533827/140‑1 (MQ=255)

                                                              cgcgATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCTGATCCGCCAGGCGATGGTAGAGTATGTTTTTGTCGGATTCGTAGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGAc                                                                         <  2:3105889/140‑1 (MQ=255)

                                                              cgcgATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGAc                                                                         <  2:3532812/140‑1 (MQ=255)

                                                              cgcgATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGGTAGTGGCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGAc                                                                         <  2:7817853/140‑1 (MQ=255)

                                                              cgcgATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGAGGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGAc                                                                         <  2:2180281/140‑1 (MQ=255)

                                                                 gATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGAcgcg                                                                      <  1:4382016/140‑1 (MQ=255)

                                                                       ggAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTAt                                                                <  1:8603759/140‑1 (MQ=255)

                                                                         agaTCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGAGGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCa                                                              <  2:5987046/140‑1 (MQ=255)

                                                                          gaTCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCAt                                                             <  1:2441823/140‑1 (MQ=255)

                                                                          gaTCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCAt                                                             <  1:2654443/140‑1 (MQ=255)

                                                                             cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATc                                                               <  2:3881008/135‑1 (MQ=255)

                                                                             cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGcc                                                          >  2:4400135/1‑140 (MQ=255)

                                                                               gacgacCTTTGTTGATCGTGAGTCGTTGGATCAGATCCGCAAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTa                                                        <  1:7642867/140‑1 (MQ=38)

                                                                               gacgacCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTa                                                        <  1:4765037/140‑1 (MQ=255)

                                                                               gacgacCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTa                                                        >  1:6985190/1‑140 (MQ=255)

                                                                               gacgacCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTa                                                        <  1:500024/140‑1 (MQ=255)

                                                                               gacgacCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTa                                                        <  2:1906162/140‑1 (MQ=255)

                                                                               gacgacCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTa                                                        <  2:8121697/140‑1 (MQ=255)

                                                                               gacgacCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTa                                                        <  1:5771421/140‑1 (MQ=255)

                                                                               cccgcccAGTGAGGATCCGGCGGCGAGGGATCCGATCCGCCCGGCGCTGGAAGAGTAGGTGATGGTCGGATGCGGCGCGCGGTGCATCCGGCACGGTGGGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTa                                                        <  2:6538674/135‑1 (MQ=25)

                                                                                 cgacCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACg                                                      <  1:4646727/140‑1 (MQ=255)

                                                                                  gacCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACgg                                                     >  1:3362752/1‑140 (MQ=255)

                                                                                      aTTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCt                                                 <  1:5352886/140‑1 (MQ=255)

                                                                                        tgatgaTCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGa                                               >  1:5496336/1‑140 (MQ=255)

                                                                                           tgatCCTGCGTCTTTGGTTCAGATCCGCCAGGCGATGGAAGTGTAGTTTATTGTCGGATGCGTCGCGCGTTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGAAGCGTTTTATCATGCCTACTGACCTGAAcc                                            <  1:1089227/140‑1 (MQ=38)

                                                                                            gatCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCg                                           <  2:1030511/140‑1 (MQ=255)

                                                                                               ccTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGGCCTGAACCGTAg                                        <  2:9460801/140‑1 (MQ=255)

                                                                                                cTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCGTGCCTACGGACCGGAACCGTAgg                                       >  1:8283324/1‑140 (MQ=255)

                                                                                                 tGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGTCGCGTTTTATCATGCCTACGGACCTGAACCGTAGGt                                      >  1:911507/1‑140 (MQ=255)

                                                                                                 tGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGt                                      >  1:9562663/1‑140 (MQ=255)

                                                                                                    gtcgtTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTGATCATGCCTACGGACCTGAACCGTAGGTCgg                                   >  2:8313429/1‑140 (MQ=255)

                                                                                                       gtTGGATCAGATCCGCCAGGCGATGGAAGAGGAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATa                                <  2:2633633/140‑1 (MQ=255)

                                                                                                        ttGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATaa                               >  1:10519640/1‑140 (MQ=255)

                                                                                                            aTCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGgcg                           <  1:11493877/140‑1 (MQ=255)

                                                                                                              cAGATCCGCCAGGCGATGGAAGAGTAGGTTAGTGACGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCAGGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCt                         <  2:7425280/140‑1 (MQ=255)

                                                                                                                gATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTcg                       >  2:1048535/1‑140 (MQ=255)

                                                                                                                gATCCGCCAGGCGATGGAAGAGTAGGGTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTcg                       <  1:6671002/140‑1 (MQ=255)

                                                                                                                  tCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTcgcg                     <  1:379843/140‑1 (MQ=255)

                                                                                                                    cGCCAGGCTATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGATTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTc                   <  2:530847/140‑1 (MQ=255)

                                                                                                                    cGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTc                   <  1:7317508/140‑1 (MQ=255)

                                                                                                                    cGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTc                   <  2:1548405/140‑1 (MQ=255)

                                                                                                                    cGCCAGGCGATGGAAGAGTAGGTTATTGTCGGAGGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGAGGCCGGATGCGACGCTGACGCGAGTTATCATGCCTACGGACCAGAACCGTAGGTCGGATAAGGCGCTCGCGTc                   <  2:7205174/140‑1 (MQ=255)

                                                                                                                     gCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCg                  >  1:10417426/1‑140 (MQ=255)

                                                                                                                      ccAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGc                 <  1:4088710/140‑1 (MQ=255)

                                                                                                                           cGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTc                   >  1:2030183/1‑133 (MQ=255)

                                                                                                                           cGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTc                   <  2:2030183/133‑1 (MQ=255)

                                                                                                                             ctGGAAGCGTAGGCTATTGTCGGAGGCGTCGCGCGGTGCATCCGGCACAGTCTGCCGATGCCTGATGCGACGCTGACGCGTATGATCAAGCCTACGGACCTGCACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGac          <  2:11228821/139‑1 (MQ=25)

                                                                                                                             aTGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGac          >  1:10165159/1‑140 (MQ=255)

                                                                                                                              tGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGaca         <  1:8790167/140‑1 (MQ=255)

                                                                                                                              tGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGaca         <  2:87429/140‑1 (MQ=255)

                                                                                                                              tGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGaca         <  2:3706635/140‑1 (MQ=255)

                                                                                                                                   gagTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATg    <  1:1069945/140‑1 (MQ=255)

                                                                                                                                   gagTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATg    <  1:7499263/140‑1 (MQ=255)

                                                                                                                                    agTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGc   >  1:3451920/1‑140 (MQ=255)

                                                                                                                                    agTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGc   >  2:9537671/1‑140 (MQ=255)

                                                                                                                                     gTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGAc                                                                         >  1:1638035/1‑69 (MQ=255)

AATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGCT  >  NZ_CP025268/353507‑353779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`