BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,743,921	T→C	I172I (ATT→ATC)	galK →	galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,743,921	0	T	C	99.8%	2035.9 / ‑5.3	584	I172I (ATT→ATC)	galK	galactokinase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (280/303); total (281/303)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 584 total aligned reads displayed.

TTAAGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATTATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTACC  >  NZ_CP025268/3743784‑3744056

ttAAGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCATCAGCTTTATCATCTGCCGCTGGACGGCGCACACATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCAt                                                                                                                                       <  2:10610293/140‑1 (MQ=255)

   aGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGa                                                                                                                                    >  1:10663587/1‑140 (MQ=255)

   aGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGa                                                                                                                                    >  2:6334970/1‑140 (MQ=255)

   aGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGa                                                                                                                                    >  2:8856575/1‑140 (MQ=255)

   aGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGa                                                                                                                                    >  1:2431320/1‑140 (MQ=255)

    gTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGAt                                                                                                                                   <  1:445582/140‑1 (MQ=255)

    gTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGAt                                                                                                                                   <  1:11209222/140‑1 (MQ=255)

        ttcCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGc                                                                                                                               >  1:6032987/1‑140 (MQ=255)

          ccGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTa                                                                                                                             >  2:439220/1‑140 (MQ=255)

           cGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTaa                                                                                                                            <  2:854913/140‑1 (MQ=255)

             cTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAAtt                                                                                                                          >  1:11004318/1‑140 (MQ=255)

             cTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAAtt                                                                                                                          >  1:9077661/1‑140 (MQ=255)

               tCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTc                                                                                                                        >  1:6423451/1‑140 (MQ=255)

                   tGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCcgcg                                                                                                                    <  2:6542076/140‑1 (MQ=255)

                     gAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCt                                                                                                                  <  2:11151317/140‑1 (MQ=255)

                        gTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTACCGGTCAGGCAGCAGACATCCCGTTTGTCGGCTGTAACTGCGGGATCATGGAGCAGCTAATATCCGCGCTCgg                                                                                                               >  2:7706489/1‑140 (MQ=255)

                          cgcgGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGACGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCa                                                                                                             >  2:4698199/1‑140 (MQ=255)

                          cgcgGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCa                                                                                                             >  1:510363/1‑140 (MQ=255)

                            cgGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCaag                                                                                                           <  2:6407436/140‑1 (MQ=255)

                             ggTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCaaga                                                                                                          <  1:1406025/140‑1 (MQ=255)

                             ggTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCaaga                                                                                                          <  2:152032/140‑1 (MQ=255)

                              gTCGGAACCGTATTGCAGCAGCTTTATCCTCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCaagaa                                                                                                         <  1:8144667/140‑1 (MQ=255)

                                 ggAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCaagaaaga                                                                                                      <  2:6040643/140‑1 (MQ=255)

                                      cGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATg                                                                                                 <  1:251126/140‑1 (MQ=255)

                                        tATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCGAATTTCCGCGCGCGGCACGAACGATCATGcc                                                                                               >  2:7448437/1‑140 (MQ=255)

                                         aTTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCt                                                                                              >  2:9307740/1‑140 (MQ=255)

                                            gcagcaGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGc                                                                                           <  2:10350600/140‑1 (MQ=255)

                                             cagcagCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAACGATCATGCCTTGCt                                                                                          >  1:8605551/1‑140 (MQ=255)

                                             cagcagCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCt                                                                                          >  2:3043770/1‑140 (MQ=255)

                                             cagcagCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCt                                                                                          >  1:993569/1‑140 (MQ=255)

                                                cagcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTgtt                                                                                       >  2:5009340/1‑138 (MQ=255)

                                                cagcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGAt                                                                                       >  1:7939886/1‑140 (MQ=255)

                                                cagcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGAt                                                                                       >  2:2409848/1‑140 (MQ=255)

                                                cagcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGAt                                                                                       >  2:9661149/1‑140 (MQ=255)

                                                 agcTTTATCATCTGCCGCTGGACGGCGCACAACTCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATTGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATc                                                                                      <  1:2658468/140‑1 (MQ=255)

                                                 agcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATc                                                                                      <  2:2276654/140‑1 (MQ=255)

                                                 agcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATc                                                                                      <  1:10518365/140‑1 (MQ=255)

                                                  gcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCg                                                                                     >  1:10779656/1‑140 (MQ=255)

                                                  gcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCg                                                                                     >  1:1736796/1‑140 (MQ=255)

                                                  gcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCg                                                                                     >  1:2269116/1‑140 (MQ=255)

                                                   cTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGa                                                                                    <  2:3467727/140‑1 (MQ=255)

                                                     ttATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGAtt                                                                                  <  2:9078824/140‑1 (MQ=255)

                                                      tATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTg                                                                                 <  1:5230130/140‑1 (MQ=255)

                                                      tATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTg                                                                                 <  2:1079781/140‑1 (MQ=255)

                                                          atcTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGc                                                                             >  1:8640945/1‑140 (MQ=255)

                                                          atcTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGc                                                                             <  1:460618/140‑1 (MQ=255)

                                                           tcTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCt                                                                            <  2:9780427/140‑1 (MQ=255)

                                                           tcTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCt                                                                            <  2:11457914/140‑1 (MQ=255)

                                                           acagCCGCAGGACGGCGCACAAATCGCGCTTAACCGACAGCAACCAGAACACCAGCTTGTACGCTGTAACTGCGGGATCATGGATCAGCTAATAACCGCGCTCGGCAATAAAGATCATGCCTTGCTGATCGATTGCCGCt                                                                            <  2:7250681/137‑1 (MQ=255)

                                                             tGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGGAGAAAACGAGTTTGTAGGCTGTAACTGGGGGATCATGGATGAGCTCATTTCCGCGCTCGGGAAGCCTGATCATGCCGTGCTGATCGAGTGGCGCTcg                                                                          >  2:8443479/1‑139 (MQ=255)

                                                             tGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCa                                                                          >  1:9332055/1‑140 (MQ=255)

                                                             tGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCa                                                                          >  1:11504550/1‑140 (MQ=255)

                                                             tGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCa                                                                          >  2:3564209/1‑140 (MQ=255)

                                                                cGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTg                                                                       <  1:3206184/140‑1 (MQ=255)

                                                                cGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTg                                                                       <  2:115754/140‑1 (MQ=255)

                                                                cGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCGTGCTGATCGATTGCCGCTCACTg                                                                       <  2:3765748/140‑1 (MQ=255)

                                                                  cTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTggg                                                                     <  1:7533394/140‑1 (MQ=255)

                                                                  cTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCATTGCTGATCGATTGCCGCTCACTggg                                                                     >  1:6265775/1‑140 (MQ=255)

                                                                     gACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGAc                                                                  <  1:8885895/140‑1 (MQ=255)

                                                                     gACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGAc                                                                  <  2:8059906/140‑1 (MQ=255)

                                                                       cGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCa                                                                <  2:830360/140‑1 (MQ=255)

                                                                         gcgcACAAATCGCGCTTAACGGTAAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGAACAGCTAATTTCCGCGCTCGGCACGAACGATCATGCCTTGCTGATCGATAGCCGCTCACTGGGGACCaaa                                                              >  2:136372/1‑140 (MQ=255)

                                                                          cgcACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAg                                                             >  2:5110321/1‑140 (MQ=255)

                                                                          cgcACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAg                                                             <  1:310052/140‑1 (MQ=255)

                                                                          cgcACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAg                                                             >  1:1176911/1‑140 (MQ=255)

                                                                          cgcACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAg                                                             >  1:962947/1‑140 (MQ=255)

                                                                          cgcACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAg                                                             <  1:5841168/140‑1 (MQ=255)

                                                                                 aTCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTc                                                      >  2:3249840/1‑140 (MQ=255)

                                                                                 aTCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTc                                                      >  2:1946710/1‑140 (MQ=255)

                                                                                  tCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCTAGATAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTcc                                                     <  2:8703144/140‑1 (MQ=255)

                                                                                  tCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTcc                                                     <  1:4860239/140‑1 (MQ=255)

                                                                                  tCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTcc                                                     <  1:1999587/140‑1 (MQ=255)

                                                                                  tCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTcc                                                     <  1:7276551/140‑1 (MQ=255)

                                                                                    gcgcTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCAt                                                   >  1:6786057/1‑140 (MQ=255)

                                                                                    gcgcTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATGTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGGTTCCag                                                   <  2:10974448/140‑2 (MQ=255)

                                                                                        ttAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGccc                                               >  1:7615803/1‑140 (MQ=255)

                                                                                        ttAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGccc                                               >  1:3938727/1‑140 (MQ=255)

                                                                                         tAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCa                                              >  2:10029650/1‑140 (MQ=255)

                                                                                         tAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCa                                              <  1:4321511/140‑1 (MQ=255)

                                                                                          accGGTCAGGAAGCAGCACCCCCGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTCAGTACCGCGCTCGGCCAGACAGATCATGCCATGCTGATCGAGTGCCGCTCACTGGGGACCAAAGCAGATTCCAGGCCCaa                                             <  2:2652312/138‑1 (MQ=255)

                                                                                          aaCGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATGTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCaa                                             <  2:5833288/140‑1 (MQ=255)

                                                                                               tCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGgtg                                        <  2:6766388/140‑1 (MQ=255)

                                                                                                cAGGAAGCAGAAAACCAGAATGTAGGCTGTAACTGCGGGATCATGGATCAGCAAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGgtgt                                       <  2:7005017/140‑1 (MQ=255)

                                                                                                  ggAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTgg                                     <  2:1620956/140‑1 (MQ=255)

                                                                                                        aGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAATCAGTTTCCATGCCCAAAGGTGTGGCTgtcg                               <  1:3503109/140‑1 (MQ=255)

                                                                                                        aGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcg                               <  2:5421753/140‑1 (MQ=255)

                                                                                                        aGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcg                               <  1:6510986/140‑1 (MQ=255)

                                                                                                         gAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcgt                              >  1:10108120/1‑140 (MQ=255)

                                                                                                          aaaaCCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcgtc                             <  1:5481454/140‑1 (MQ=255)

                                                                                                                 gTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGtcatcatca                      >  1:3587026/1‑140 (MQ=255)

                                                                                                                  tttGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCCAAGGTGTGGCTGTCGTCATCATCaa                     >  2:4104152/1‑140 (MQ=255)

                                                                                                                  tttGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCaa                     >  2:9393929/1‑140 (MQ=255)

                                                                                                                    tGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACa                   >  1:6380638/1‑140 (MQ=255)

                                                                                                                      tAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGt                 <  2:2551804/140‑1 (MQ=255)

                                                                                                                              aaCTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCaa         <  1:8517153/140‑1 (MQ=255)

                                                                                                                                cTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAAc       <  2:4550207/140‑1 (MQ=255)

                                                                                                                                cTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAAc       <  2:2034904/140‑1 (MQ=255)

                                                                                                                                   cGGGATCATGGATCAGCTAATTTCCTCTCTCGGCAAGAAAGATCATGACTTGATGATCGATTTCCGCTCACTGGGGACCAAATCAGTTTCCATGCCCAAAGGTGTGGATGTCGTCATCATCAACAGTAACTTCAAACGTa    >  2:6128473/1‑140 (MQ=255)

                                                                                                                                   cGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTa    >  1:2959275/1‑140 (MQ=255)

                                                                                                                                     ggATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTAcc  <  2:2865986/140‑1 (MQ=255)

TTAAGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATTATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTACC  >  NZ_CP025268/3743784‑3744056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`