Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,915,245 A→G C115R (TGT→CGT)  glmS ← glutamine‑‑fructose‑6‑phosphate transaminase (isomerizing)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,915,2450AG99.8% 3679.3 / ‑4.3 1062C115R (TGT→CGT) glmSglutamine‑‑fructose‑6‑phosphate transaminase (isomerizing)
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (577/481);  total (578/484)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 1069 total aligned reads displayed.

CACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACACGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAGCAATA  >  NZ_CP025268/3915108‑3915381
                                                                                                                                         |                                                                                                                                        
  cTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCt                                                                                                                                      >  1:10304787/1‑140 (MQ=255)
  cTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCt                                                                                                                                      >  1:5963801/1‑140 (MQ=255)
   tGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCtt                                                                                                                                     >  1:11361567/1‑140 (MQ=255)
    gTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCttt                                                                                                                                    <  2:5052355/140‑1 (MQ=255)
    gTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCttt                                                                                                                                    >  1:1745915/1‑140 (MQ=255)
      aCCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTa                                                                                                                                  <  2:3972263/140‑1 (MQ=255)
       ccGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATCGCCACGCGCTTTTAg                                                                                                                                 <  1:1885258/140‑1 (MQ=255)
        cGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGc                                                                                                                                >  1:4966422/1‑140 (MQ=255)
        cGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGc                                                                                                                                >  1:7086889/1‑140 (MQ=255)
        cGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCGCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAACCGAAGGTATAGCCACGCGCTTTTAGc                                                                                                                                <  2:2131476/140‑1 (MQ=255)
         gTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCTCTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGct                                                                                                                               <  2:7074840/140‑1 (MQ=255)
          tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGACACGTAGGTAGAGCCACGCGCTTTTAGctc                                                                                                                              >  2:6736712/1‑140 (MQ=255)
          tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                              >  1:8865886/1‑140 (MQ=255)
          tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                              >  2:2470219/1‑140 (MQ=255)
          tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                              >  1:6558243/1‑140 (MQ=255)
          tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                              >  1:1115346/1‑140 (MQ=255)
          tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                              >  1:3099150/1‑140 (MQ=255)
          tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                              >  2:7465459/1‑140 (MQ=255)
          tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTGCAGCTCCCAGTTCACCAGATGGGCACTCTCTTCGGTGTCGGTTTCAGAAACGAAGGTCGAGCCACGCGCTTTTAGatc                                                                                                                              >  2:7655801/1‑140 (MQ=255)
           aCGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctct                                                                                                                             >  2:10621842/1‑140 (MQ=255)
           aCGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctct                                                                                                                             >  2:5524302/1‑140 (MQ=255)
            cGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCtt                                                                                                                            >  2:3003782/1‑140 (MQ=255)
             gCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTc                                                                                                                           >  1:1000885/1‑140 (MQ=255)
             gCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTc                                                                                                                           >  1:257433/1‑140 (MQ=255)
             gCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTc                                                                                                                           >  1:9692801/1‑140 (MQ=255)
             gCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTc                                                                                                                           >  1:11622218/1‑140 (MQ=255)
               accacGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCAc                                                                                                                         >  1:2118780/1‑140 (MQ=255)
                 cacGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGc                                                                                                                       >  1:10961550/1‑140 (MQ=255)
                 cacGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGc                                                                                                                       >  1:2284250/1‑140 (MQ=255)
                  acGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCa                                                                                                                      <  1:8685396/140‑1 (MQ=255)
                  acGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCa                                                                                                                      >  2:10338082/1‑140 (MQ=255)
                   cGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAg                                                                                                                     >  1:1583811/1‑140 (MQ=255)
                    gCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGc                                                                                                                    >  2:4409398/1‑140 (MQ=255)
                       gCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGGTTTTAGCTCTTCTCGCAGCGGt                                                                                                                 >  1:9118098/1‑140 (MQ=255)
                          gCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCa                                                                                                              <  2:7312027/140‑1 (MQ=255)
                           cGGGATAGCTCGCAGAACGGCCTCCCGCAGTGTCCCGCCTTGTTTCAGCTCCATGTTCTCCAGATGGGCAATCACTGCGGTGTCGGTTTCAGAATCGTAGGTTTTGGCACGCGGATTTAGCTCTTCACGCTGCGGTTCag                                                                                                             >  2:6155033/1‑139 (MQ=255)
                           cGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCAt                                                                                                             <  2:4117590/140‑1 (MQ=255)
                               aTAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGtt                                                                                                         <  1:457792/140‑1 (MQ=255)
                                  gCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTc                                                                                                      >  1:4155407/1‑140 (MQ=255)
                                     cGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCgat                                                                                                   <  2:9633957/140‑1 (MQ=255)
                                     cGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCgat                                                                                                   >  2:7834332/1‑140 (MQ=255)
                                       cAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCgatga                                                                                                 <  1:7791646/140‑1 (MQ=255)
                                       cAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCgatga                                                                                                 <  1:4028862/140‑1 (MQ=255)
                                        aGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCTATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCgatgat                                                                                                <  1:9255392/140‑1 (MQ=255)
                                        aGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCgatgat                                                                                                >  1:6404160/1‑140 (MQ=255)
                                        aGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACGTCGGTGTCGGTGTCAGAAACGAAGGTCTAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCgaggat                                                                                                >  2:8502998/1‑140 (MQ=255)
                                         gAACTGCCTCACTCAGAGTCCCGCCTTGTTTCAGCTCCCTGTTCACCATATGTGCAATCAGTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCAACGCGCTTTTAGCTCTTCACGCAGCGTTTCATGGTTTTCGttgatg                                                                                               <  2:1396182/140‑1 (MQ=255)
                                         gAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGatgatg                                                                                               <  2:5234338/140‑1 (MQ=255)
                                         gAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGatgatg                                                                                               <  2:11265940/140‑1 (MQ=255)
                                         gAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGatgatg                                                                                               <  2:9760877/140‑1 (MQ=255)
                                         gAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGatgatg                                                                                               <  1:2883987/140‑1 (MQ=255)
                                          aaCGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGc                                                                                              >  1:5720324/1‑140 (MQ=255)
                                          aaCGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGc                                                                                              >  2:1807109/1‑140 (MQ=255)
                                            cGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCg                                                                                            >  2:8255823/1‑140 (MQ=255)
                                               ccTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTa                                                                                         <  2:6387762/140‑1 (MQ=255)
                                               ccTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTa                                                                                         <  2:6558242/140‑1 (MQ=255)
                                               ccTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTa                                                                                         <  2:3092915/140‑1 (MQ=255)
                                               ccTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTa                                                                                         <  1:4342595/140‑1 (MQ=255)
                                               ccTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGGTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTa                                                                                         <  1:10539637/140‑1 (MQ=255)
                                                   aCGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGca                                                                                     <  1:5206702/140‑1 (MQ=255)
                                                   aCGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGca                                                                                     <  2:2824981/140‑1 (MQ=255)
                                                   aCGCAGAGTCCCGCCTTGTTACCTCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTACATAAACGTAGGTATAGCCACGCGCTTTTAGCTCGTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGca                                                                                     <  2:2290366/140‑1 (MQ=255)
                                                    cGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGcac                                                                                    >  1:9498376/1‑140 (MQ=255)
                                                    cGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGGTATGcac                                                                                    >  2:9160810/1‑140 (MQ=255)
                                                      cAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGcacca                                                                                  <  2:5331963/140‑1 (MQ=255)
                                                        gagTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGcaccacc                                                                                <  1:7618284/140‑1 (MQ=255)
                                                        gagTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGAAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGcaccacc                                                                                <  2:11528842/140‑1 (MQ=255)
                                                             ccGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAAt                                                                           <  1:3525455/140‑1 (MQ=255)
                                                             ccGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGGTTTCGATGATGCCGTTATGCACAACCACAAt                                                                           >  2:4665227/1‑140 (MQ=255)
                                                             ccGCCTTGTTTCAGCTCCCAGTTCACCAGATGCGCAATCACGTCGGTGTCGGTTTCAGAACCGAAGGTATAGCCACGCGCTGTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGCTGATGCCGTTATGCACCACcacact                                                                           <  2:6004680/140‑3 (MQ=255)
                                                              cGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAAtg                                                                          <  2:11360432/140‑1 (MQ=255)
                                                                   tGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTc                                                                     >  1:8516103/1‑140 (MQ=255)
                                                                   tGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTc                                                                     >  2:8717569/1‑140 (MQ=255)
                                                                   tGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTc                                                                     <  2:1247814/140‑1 (MQ=255)
                                                                           cTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACAt                                                             >  1:5576937/1‑140 (MQ=255)
                                                                              ccAGTTCACCAGATGGGCAACCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCg                                                          <  2:3795279/140‑1 (MQ=255)
                                                                               cAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCgg                                                         >  1:3267775/1‑140 (MQ=255)
                                                                                       cAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCAt                                                 >  2:8957942/1‑140 (MQ=255)
                                                                                       cAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCAt                                                 >  2:6905100/1‑140 (MQ=255)
                                                                                        aGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCAtt                                                <  1:277434/140‑1 (MQ=255)
                                                                                        aGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCAtt                                                <  2:3302714/140‑1 (MQ=255)
                                                                                          aTGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCa                                              <  1:9906156/140‑1 (MQ=255)
                                                                                            gggCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACt                                            >  2:10776208/1‑140 (MQ=255)
                                                                                                aaTCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTg                                        <  1:5373635/140‑1 (MQ=255)
                                                                                                 aTCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGa                                       <  2:5665300/140‑1 (MQ=255)
                                                                                                 aTCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGa                                       >  1:10634236/1‑140 (MQ=255)
                                                                                                 aTCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGa                                       <  1:7429363/140‑1 (MQ=255)
                                                                                                    aCTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACATCTGAAgg                                    >  2:3683544/1‑140 (MQ=255)
                                                                                                    aCTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCAGGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAgg                                    >  2:10997974/1‑140 (MQ=255)
                                                                                                         ggTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGAGCATTAACATCTGAATGTTCAc                               >  2:9338283/1‑140 (MQ=255)
                                                                                                            gtCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGt                            >  2:4850067/1‑140 (MQ=255)
                                                                                                             tCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTg                           >  1:8215379/1‑140 (MQ=255)
                                                                                                             tCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTg                           >  1:4752362/1‑140 (MQ=255)
                                                                                                                  gtCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCg                      <  2:7592245/139‑1 (MQ=255)
                                                                                                                       aaaCGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAg                 >  1:6781747/1‑140 (MQ=255)
                                                                                                                          cGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGa              <  2:1583811/140‑1 (MQ=255)
                                                                                                                            aaGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAgt            <  1:6100358/140‑1 (MQ=255)
                                                                                                                                 ataGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAg       <  2:2612341/140‑1 (MQ=255)
                                                                                                                                 ataGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAg       <  1:3771507/140‑1 (MQ=255)
                                                                                                                                     ccACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAGCAAt   >  2:809362/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
CACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACACGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAGCAATA  >  NZ_CP025268/3915108‑3915381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑