Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,958,794 A→G *515* (TAA→TAG)  ilvA → threonine ammonia‑lyase, biosynthetic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,958,7940AG99.3% 1466.5 / NA 428*515* (TAA→TAG) ilvAthreonine ammonia‑lyase, biosynthetic
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (219/206);  minor base T (1/2);  total (220/208)
Fisher's exact test for biased strand distribution p-value = 6.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 429 total aligned reads displayed.

ACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAAGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCA  >  NZ_CP025268/3958657‑3958930
                                                                                                                                         |                                                                                                                                        
   gACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGACCCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGATCCGGTGATTTTTGGCGGGTGAGGGaca                                                                                                                                     >  2:6898779/1‑138 (MQ=255)
   gACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGaaa                                                                                                                                     >  1:571874/1‑140 (MQ=255)
   gACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGaaa                                                                                                                                     >  2:1696509/1‑140 (MQ=255)
   gACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGaaa                                                                                                                                     >  1:1653932/1‑140 (MQ=255)
    aCTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGaaaa                                                                                                                                    >  2:9343112/1‑140 (MQ=255)
    aCTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGaaaa                                                                                                                                    >  1:8539515/1‑140 (MQ=255)
    aCTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGaaaa                                                                                                                                    <  2:3952643/140‑1 (MQ=255)
    aCTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTGCAGGTTCTTGTTGGCGGGTTAGGGaaaa                                                                                                                                    >  2:5113540/1‑140 (MQ=255)
      tACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAAt                                                                                                                                  >  1:7407254/1‑140 (MQ=255)
       ccGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGCACCGGATTTCGAAACCCGGCTGACTGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATg                                                                                                                                 <  2:475059/139‑1 (MQ=255)
         gggCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAATTGcc                                                                                                                               >  2:1063375/1‑140 (MQ=255)
            cgcgTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGa                                                                                                                            <  1:6495274/140‑1 (MQ=255)
              cgTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGCTTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATATCCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGAtc                                                                                                                          >  2:7131561/1‑139 (MQ=255)
              cgTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATa                                                                                                                          >  2:5417480/1‑140 (MQ=255)
              cgTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATa                                                                                                                          >  1:547660/1‑140 (MQ=255)
              cgTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATa                                                                                                                          >  1:3371319/1‑140 (MQ=255)
              cgTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATa                                                                                                                          >  1:4594731/1‑140 (MQ=255)
               gTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAg                                                                                                                         >  1:6752377/1‑140 (MQ=255)
                tACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAgc                                                                                                                        >  2:2837183/1‑140 (MQ=255)
                 aCTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAgcg                                                                                                                       <  2:7009441/140‑1 (MQ=255)
                 aCTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAgcg                                                                                                                       <  1:11533904/140‑1 (MQ=255)
                 aCTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAgcg                                                                                                                       <  1:11257221/140‑1 (MQ=255)
                 aCTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAgcg                                                                                                                       <  2:6593112/140‑1 (MQ=255)
                     gcggcgTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGcgcttc                                                                                                                   >  1:3926266/1‑140 (MQ=255)
                      cggcgTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGcgcttcg                                                                                                                  <  2:7416489/140‑1 (MQ=255)
                          gTTCGAACTTGGCGACCCAGAACCCGATTTCGAAACCCGGCTGAATGAGCTGGGCGACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTa                                                                                                              <  2:10271698/140‑1 (MQ=255)
                           ttCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAATATGCCTGATAGCGCTTCGCTTAt                                                                                                             >  2:1011183/1‑140 (MQ=255)
                           ttCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTAt                                                                                                             <  2:4520525/140‑1 (MQ=255)
                           ttCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTAt                                                                                                             <  1:5092707/140‑1 (MQ=255)
                           gtCGAACTTGGCGGCCAGGAACCGGAGTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGCACCCACTCACCCGGCGTGCAGGGTCTTGTTGGCGGGGGCGGGAAAGATGCCTGATAGggctacggggag                                                                                                             >  2:10683055/2‑135 (MQ=255)
                           cgcGATCTTGTCGCCCATGAACCGGATTTCGAACCCCGGCTGACTGAGCTGGGCTACGATTGCCACGACGAAACCACGACCCCCGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCatat                                                                                                             <  2:8847418/138‑1 (MQ=255)
                             cGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCCGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCGTCGCGTATcg                                                                                                           >  1:8962161/1‑139 (MQ=255)
                                   tGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTa                                                                                                     <  2:5281148/140‑1 (MQ=255)
                                   tGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTa                                                                                                     <  2:2278431/140‑1 (MQ=255)
                                     gCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTAcc                                                                                                   >  2:7556253/1‑140 (MQ=255)
                                     gCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTAcc                                                                                                   >  2:3792599/1‑140 (MQ=255)
                                         ccATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCcgcg                                                                                               <  1:3581383/140‑1 (MQ=255)
                                         ccATGAACCGGAGTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCTCGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCcgcg                                                                                               <  1:9297802/140‑1 (MQ=255)
                                            tGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGa                                                                                            >  1:2385494/1‑140 (MQ=255)
                                             gAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATACCCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGAc                                                                                           <  1:9807273/140‑1 (MQ=255)
                                                   gATTTCGAAACCCGGCTGAATGAGCTGGGCTACGCTTGCCACGACGAAACCAATATCCCGGCGTTCAGGTTCTTTTTGGCGGGTGAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACggc                                                                                     >  2:11435529/1‑138 (MQ=255)
                                                   gATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTc                                                                                     >  1:6970854/1‑140 (MQ=255)
                                                        cGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAATATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTtg                                                                                >  2:4705290/1‑140 (MQ=255)
                                                            aCCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGt                                                                            <  1:10177309/140‑1 (MQ=255)
                                                             cccGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGtt                                                                           <  1:53690/140‑1 (MQ=255)
                                                                  cTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCa                                                                      <  2:6167245/140‑1 (MQ=255)
                                                                  cTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCa                                                                      <  1:7661797/140‑1 (MQ=255)
                                                                    gAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCaaa                                                                    <  2:1356903/140‑1 (MQ=255)
                                                                    gAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCaaa                                                                    <  2:195958/140‑1 (MQ=255)
                                                                       tGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATc                                                                 <  2:8043046/140‑1 (MQ=255)
                                                                             gggCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGTTTCTTTTTGGCGGGTTATGGAAAAATGCCTGATAGCGCTACGCTTATCAGGCCTACCCGCGCGACAACGACATTTGTGGTTCGGCAAAATCTTCCAg                                                           <  2:3634224/140‑1 (MQ=255)
                                                                              ggCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATCGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGa                                                          <  1:10054544/140‑1 (MQ=255)
                                                                              ggCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGa                                                          <  1:10797221/140‑1 (MQ=255)
                                                                               gCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGaa                                                         >  1:6142737/1‑140 (MQ=255)
                                                                               gCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGaa                                                         >  2:966766/1‑140 (MQ=255)
                                                                               gCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGGCATTTGTGGTTCGGCAAAATCTTCCAGaa                                                         >  1:182304/1‑140 (MQ=255)
                                                                                cTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAAt                                                        <  2:4953049/140‑1 (MQ=255)
                                                                                 tACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATg                                                       <  1:4392709/140‑1 (MQ=255)
                                                                                   cGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCGTCGCTGATCAGGCCTACCCGCGCGACAACGGCATGTGTGGTTCGGCAAAATCTTCCAGAATGcc                                                     >  1:1403652/1‑140 (MQ=255)
                                                                                    gATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCt                                                    <  2:8219466/140‑1 (MQ=255)
                                                                                        gCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAAt                                                >  2:4294201/1‑140 (MQ=255)
                                                                                        gCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAAt                                                >  1:8799355/1‑140 (MQ=255)
                                                                                         ccACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAAtt                                               >  1:9160666/1‑140 (MQ=255)
                                                                                          cACGACGAAACCAATTACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTa                                              <  2:7932422/140‑1 (MQ=255)
                                                                                          cACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTa                                              <  1:403598/140‑1 (MQ=255)
                                                                                           acgacgAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAg                                             <  1:4804372/140‑1 (MQ=255)
                                                                                              acgaAACCAATAATCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCgg                                          >  1:2854847/1‑140 (MQ=255)
                                                                                               cgaAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGc                                         <  1:10568195/140‑1 (MQ=255)
                                                                                                gaAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCt                                        >  2:5896909/1‑140 (MQ=255)
                                                                                                gaAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCAGTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCt                                        >  2:3324729/1‑140 (MQ=255)
                                                                                                      aaTAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGCCAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTa                                  <  1:8289848/140‑1 (MQ=255)
                                                                                                      aaTAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTa                                  <  1:8053442/140‑1 (MQ=255)
                                                                                                        tAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGc                                >  1:1522680/1‑140 (MQ=255)
                                                                                                        tAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGc                                <  1:3237315/140‑1 (MQ=255)
                                                                                                           cccGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGc                             <  2:10868984/140‑1 (MQ=255)
                                                                                                             cGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCtt                           <  2:11121636/140‑1 (MQ=255)
                                                                                                             cGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCtt                           >  1:3017799/1‑140 (MQ=255)
                                                                                                              ggCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCttt                          >  1:11067899/1‑140 (MQ=255)
                                                                                                               gCGTTCAGGTTCTTTTTGGTGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCAGCTCATGTAGCCGCtttt                         >  2:2930053/1‑140 (MQ=255)
                                                                                                                ctttaaGGTTCTTTTTGGCGGGTTAGGGAAATATGCCTGATAGCGCTTAGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCTAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTATCCGCttttt                        <  2:3060289/135‑1 (MQ=255)
                                                                                                                     aGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTgcg                   <  1:9536489/140‑1 (MQ=255)
                                                                                                                     aGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTgcg                   <  1:9432732/140‑1 (MQ=255)
                                                                                                                     aGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTgcg                   <  2:2156732/140‑1 (MQ=255)
                                                                                                                     aGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTgcg                   <  2:9174272/140‑1 (MQ=255)
                                                                                                                     aGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTgcg                   <  1:5190310/140‑1 (MQ=255)
                                                                                                                          cTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGcacac              >  1:3696409/1‑140 (MQ=255)
                                                                                                                          cTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGcacac              >  2:892237/1‑140 (MQ=255)
                                                                                                                          cTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGcacac              <  2:9460871/140‑1 (MQ=255)
                                                                                                                          cTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCACGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGcacac              >  2:1225256/1‑140 (MQ=255)
                                                                                                                            ttttGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCacacac            >  2:4576036/1‑140 (MQ=255)
                                                                                                                            ttttGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCacacac            >  1:8398413/1‑140 (MQ=255)
                                                                                                                             tttGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACg           >  1:780863/1‑140 (MQ=255)
                                                                                                                              ttGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGc          <  2:1592591/140‑1 (MQ=255)
                                                                                                                               tGGCGGGTTAGGGAAAAATTCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCCCACACGcc         <  2:2683934/140‑1 (MQ=255)
                                                                                                                                 gCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCa       >  1:1219106/1‑140 (MQ=255)
                                                                                                                                    ggTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTTGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTATTTCTGCGCACACACGCCCAGCt    >  2:6027424/1‑140 (MQ=255)
                                                                                                                                     gTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTc   >  2:10408125/1‑140 (MQ=255)
                                                                                                                                     gTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTc   >  2:7218918/1‑140 (MQ=255)
                                                                                                                                     gTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTc   >  1:2064444/1‑140 (MQ=255)
                                                                                                                                      ttAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCa  >  1:3075018/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
ACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAAGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTCA  >  NZ_CP025268/3958657‑3958930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑