BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,026,258	A→G	261 (TAA→TAG) L162L (CTT→CTC)	tatD → rfaH ←	3'‑5' ssDNA/RNA exonuclease TatD transcription/translation regulatory transformer protein RfaH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,026,258	0	A	G	99.7%	2497.1 / NA	714	261 (TAA→TAG) L162L (CTT→CTC)	tatD rfaH	3'‑5' ssDNA/RNA exonuclease TatD transcription/translation regulatory transformer protein RfaH
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (264/448); minor base T (0/2); total (264/450)
Fisher's exact test for biased strand distribution p-value = 5.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 715 total aligned reads displayed.

CCATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCGGTAATA  >  NZ_CP025268/4026121‑4026393

    catcCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTg                                                                                                                                   <  1:8705706/140‑1 (MQ=255)

         cGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGaa                                                                                                                              >  2:5339776/1‑140 (MQ=255)

          ggCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAAc                                                                                                                             >  1:936733/1‑140 (MQ=255)

          ggCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAAc                                                                                                                             >  2:2558595/1‑140 (MQ=255)

           gcgcAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACt                                                                                                                            <  1:7233702/140‑1 (MQ=255)

             gcAACGAGCCAGCCCATCTGCCCCATATTTTGCACCGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTCGCTGCCACCACGGATGCTAATGTCAAAACACTGATTGGGATTGCGTTTTAGAGTTTGCGGAACTCg                                                                                                                          <  2:10286391/140‑1 (MQ=255)

             gcAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCg                                                                                                                          <  2:6112761/140‑1 (MQ=255)

             gcAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCg                                                                                                                          <  1:9851474/140‑1 (MQ=255)

               atcGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGt                                                                                                                        <  1:7838790/138‑1 (MQ=255)

               aaCGAGCCAGCCCATCTGCCCCATATTTTGCTACGTAGTGCGCACTGGCGTTGAGAAGATGCCGCATGGCTGGCTGCCACAACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTTCGGAACTCTGt                                                                                                                        <  2:325983/140‑1 (MQ=255)

               aaCGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGt                                                                                                                        <  1:10600340/140‑1 (MQ=255)

               aaCGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGt                                                                                                                        <  1:6881958/140‑1 (MQ=255)

               aaCGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGt                                                                                                                        <  1:5688924/140‑1 (MQ=255)

               aaCGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGt                                                                                                                        <  1:4896938/140‑1 (MQ=255)

               aaCGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGt                                                                                                                        <  1:4736252/140‑1 (MQ=255)

               aaCGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGt                                                                                                                        <  1:4538554/140‑1 (MQ=255)

               aaCGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGt                                                                                                                        <  1:3990053/140‑1 (MQ=255)

               aaCGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGATTGCGGAACTCGGt                                                                                                                        <  2:9827864/140‑1 (MQ=255)

                 cGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTAt                                                                                                                      <  1:6388664/140‑1 (MQ=255)

                    gccagccCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTAttct                                                                                                                   <  1:10046885/140‑1 (MQ=255)

                    gccagccCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTAttct                                                                                                                   <  2:4801901/140‑1 (MQ=255)

                    gccagccCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTAttct                                                                                                                   <  2:10985006/140‑1 (MQ=255)

                    gccagccCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTAttct                                                                                                                   <  1:9203605/140‑1 (MQ=255)

                         cccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                              <  1:3271290/140‑1 (MQ=255)

                         cccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                              <  2:2461993/140‑1 (MQ=255)

                         cccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                              <  2:1037633/140‑1 (MQ=255)

                         cccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                              <  1:2463686/140‑1 (MQ=255)

                         cccATCTGCCCCATATTTTGCAACGTAGTGCGCACTGGCGTGGAGTAGATGCCGCATGGCTGGCTGCCACCACGGATGCTACTGTCAAAACACTGTTTGGGAGTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                              <  2:2962202/140‑1 (MQ=255)

                          ccATCTGCCCCATATTTTGCACCGTAGTGCGCACTGGCGTGGAGAAGTTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                             <  1:540700/140‑1 (MQ=255)

                          ccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                             <  2:7102581/140‑1 (MQ=255)

                          ccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                             <  2:8080625/140‑1 (MQ=255)

                          ccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                             <  2:4604047/140‑1 (MQ=255)

                          ccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGAGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTATAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                             <  2:4402685/140‑1 (MQ=255)

                          ccATCTGCCCCAGATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTCATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                             <  2:8907969/140‑1 (MQ=255)

                           cATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACt                                                                                                            <  1:2511224/140‑1 (MQ=255)

                                   ccATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGGTTGGGATGGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTaa                                                                                                    >  2:5039194/1‑140 (MQ=255)

                                     atatTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAAtc                                                                                                  >  1:1476496/1‑140 (MQ=255)

                                      tatTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAAtct                                                                                                 >  1:2069196/1‑140 (MQ=255)

                                      tatTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAAtct                                                                                                 <  2:1964596/140‑1 (MQ=255)

                                      tatTGTGCAACGTAGTGCGCTCTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTTATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAAtct                                                                                                 <  2:3480712/140‑1 (MQ=255)

                                           tGCAACGTATTGCGCCCTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAAGGTCAAATCACTGTGTGGGATTGCGTGTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTATTCTCTtta                                                                                            >  2:1083624/1‑140 (MQ=255)

                                           tGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTtta                                                                                            >  1:1257473/1‑140 (MQ=255)

                                           tGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTtta                                                                                            >  1:1769201/1‑140 (MQ=255)

                                           tGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTtta                                                                                            >  2:5800715/1‑140 (MQ=255)

                                           tGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACAACGGATGCTAATGTCAAAACACTGTTTGGGAGTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTtta                                                                                            >  2:6585122/1‑140 (MQ=255)

                                               aCGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaa                                                                                        <  2:7406708/140‑1 (MQ=255)

                                               aCGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaa                                                                                        <  1:3853425/140‑1 (MQ=255)

                                               aCGTAGTGCGCACTGGCGTGGAGAAGAGGCCGCATGGCTGGCTGCCACCACGGATGCAAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaa                                                                                        <  1:7510862/140‑1 (MQ=255)

                                                cGTTTTGCGCACTGGAGTGGATAATATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACAATGTTTGGGATTGCGTTTTAGAGTTTGCGCAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaat                                                                                       >  2:11593998/1‑140 (MQ=255)

                                                cGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaat                                                                                       <  2:8295796/140‑1 (MQ=255)

                                                cGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaat                                                                                       <  1:1048758/140‑1 (MQ=255)

                                                    ttGCGCACTGGCGTGGAGAAGATGCCTCATGGCTGGCTGCCACCACGGATGCTAAGGTCAAAACACTGTTTGGGAGTGCGTTTTAGAGTTTGCGGAACTCGGTATTCGTCACACTGTGCTTAATCTCTTTATTAATAAGa                                                                                   <  2:6875364/140‑1 (MQ=255)

                                                    ttGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACCCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGa                                                                                   <  1:3150485/140‑1 (MQ=255)

                                                    gtGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGa                                                                                   <  2:8606348/139‑1 (MQ=255)

                                                    gtGCGCACTGGCGAGGAGAAGATGCCGCATGGCTGGCTGCCACCCCGGATGCTACTGACAAAACACTGTTTGGGCGTGCGTTTTCGAGTTTGCGGAACTCGGGATTCTTCACACTGGGCTGAATCTCTTTATTAATAAGa                                                                                   <  2:6414271/139‑1 (MQ=255)

                                                     tGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGAt                                                                                  >  2:786368/1‑140 (MQ=255)

                                                       cgcACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTa                                                                                <  1:2905756/140‑1 (MQ=255)

                                                       cgcACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTa                                                                                <  1:7951336/140‑1 (MQ=255)

                                                       cgcACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTa                                                                                <  2:398120/140‑1 (MQ=255)

                                                         cACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAg                                                                              <  1:8164524/140‑1 (MQ=255)

                                                           cTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCa                                                                            <  1:348829/140‑1 (MQ=255)

                                                           cTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCa                                                                            <  1:1559241/140‑1 (MQ=255)

                                                           cTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCa                                                                            <  1:1888626/140‑1 (MQ=255)

                                                            tGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACCCTGTTTGGGATTGCGTGTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCaa                                                                           >  2:5443703/1‑140 (MQ=255)

                                                            tGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                             >  2:7595412/1‑106 (MQ=255)

                                                            tGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCaa                                                                           >  2:2360077/1‑140 (MQ=255)

                                                            tGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCaa                                                                           >  1:8484949/1‑140 (MQ=255)

                                                            tGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCaa                                                                           >  2:1619709/1‑140 (MQ=255)

                                                            tGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCaa                                                                           >  1:6485655/1‑140 (MQ=255)

                                                               cGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAg                                                                        >  1:8967712/1‑140 (MQ=255)

                                                                 tGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCa                                                                      >  2:7705036/1‑140 (MQ=255)

                                                                 tGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCa                                                                      >  2:11376905/1‑140 (MQ=255)

                                                                     gaagcTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTCGAGTTTGCGGAACTCGGTATTCTTCACCCTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGga                                                                  <  2:11164608/140‑1 (MQ=255)

                                                                        gaTGCCGCATGGCTGGCTGCCACCACGGCTGCTAATGTCAAAACACTGTTTGGGATTGCGTTGTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTACTCTCTTTATTAATAAGATTAAGCAATAGCAGGgagcg                                                               >  2:10615089/1‑140 (MQ=255)

                                                                        gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGgagcg                                                               <  1:7704806/140‑1 (MQ=255)

                                                                                   gctggctgCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCACTAGCATGGAGCGAGCCTCACCAt                                                    >  2:7234436/1‑140 (MQ=255)

                                                                                   gctggctgCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCAt                                                    >  1:11124759/1‑140 (MQ=255)

                                                                                      ggctgCCACCACGGCTGCGAATGTCAAAACACTGTTTGGGATTGCGTGTTAGAGTTTGCGGCACTCGGTATTCTTCTCACTGTGCTTAATCTCTTTATTAATAAGAGTAAGCAATAGCATGGAGCGAGCCTCACCATCgg                                                 >  2:5526215/1‑140 (MQ=255)

                                                                                       gctgCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                             >  1:7133088/1‑79 (MQ=255)

                                                                                        ctgCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGt                                               <  1:967934/140‑1 (MQ=255)

                                                                                                  ggATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGaaaa                                     >  1:10356397/1‑140 (MQ=255)

                                                                                                         caTGTCAAAACACTGTTTGGGATTGCGGTTTAGAGTTTGCGGAACTCGGTATTCGTCACTCTGTGCGTCATCGCTTTATTATTTAGAGTAAGCAAGAGCATGGAGCGAGCCTCACCAGCGGGGTCGGTGCAAATGGCCTg                              >  2:10023663/2‑140 (MQ=255)

                                                                                                          aTGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGa                             <  1:5241211/140‑1 (MQ=255)

                                                                                                          aTGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGa                             <  1:510459/140‑1 (MQ=255)

                                                                                                               aaaaCACTGTTTGGGATTTCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGcc                        >  2:4158561/1‑140 (MQ=255)

                                                                                                                aaaCACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCg                                                                                                                          >  2:1506356/1‑41 (MQ=255)

                                                                                                                aaaCACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGt                                                                                                                        >  2:623151/1‑43 (MQ=255)

                                                                                                                aaaCACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGt                                                                                                                        <  1:623151/43‑1 (MQ=255)

                                                                                                                aaaCACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCt                       <  1:11421983/140‑1 (MQ=255)

                                                                                                                  acacTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTc                     <  2:3672762/140‑1 (MQ=255)

                                                                                                                         ttGGGATTGCGTTTGAGAGTTTGCGGAACTCGGTATTCTTCACACGGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAAcgcg              <  2:9419842/140‑1 (MQ=255)

                                                                                                                             gATTGCGTTTTAGAGTTTGCGGAACTCGTTATTCTTCACAATGTGCTTAATCTCTTTATTAATAAGATTAAGAAATAGCATGGAGAGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCtt          >  2:2768739/1‑140 (MQ=255)

                                                                                                                                tGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGa                                                                                   <  1:11165997/64‑1 (MQ=255)

                                                                                                                                tGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCgg       >  1:4220965/1‑140 (MQ=255)

                                                                                                                                tGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCgg       <  1:5956945/140‑1 (MQ=255)

                                                                                                                                tGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCgg       <  1:10811318/140‑1 (MQ=255)

                                                                                                                                 gCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCGGt      <  2:9140660/140‑1 (MQ=255)

                                                                                                                                  cGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCGGta     >  2:3802679/1‑140 (MQ=255)

                                                                                                                                  cGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCGGta     >  1:6619481/1‑140 (MQ=255)

                                                                                                                                     tttAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCGGtaata  <  1:2744151/140‑1 (MQ=255)

CCATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCGGTAATA  >  NZ_CP025268/4026121‑4026393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`