Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 4,206,567 A→G *442* (TAA→TAG) 
N429N (AAT→AAC) 		 zraR →
purD ←		 sigma‑54‑dependent response regulator transcription factor ZraR
phosphoribosylamine‑‑glycine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252684,206,5670AG100.0% 2080.5 / NA 595*442* (TAA→TAG) 
N429N (AAT→AAC) 		zraR
purD		sigma‑54‑dependent response regulator transcription factor ZraR
phosphoribosylamine‑‑glycine ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (204/391);  total (204/391)

Only 100 of 595 total aligned reads displayed.

CAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGCACAGTA  >  NZ_CP025268/4206430‑4206703
                                                                                                                                         |                                                                                                                                        
 aGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTCTTGGCAAAACTGTCGCGTTAGTTc                                                                                                                                       >  2:8618974/1‑140 (MQ=255)
 aGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTc                                                                                                                                       >  1:3942398/1‑140 (MQ=255)
 aGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTc                                                                                                                                       >  2:3590594/1‑140 (MQ=255)
    atatTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGc                                                                                                                                    <  1:9188549/140‑1 (MQ=255)
    atatTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGc                                                                                                                                    <  1:8369122/140‑1 (MQ=255)
    atatTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGc                                                                                                                                    <  1:6953440/140‑1 (MQ=255)
      atTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTc                                                                                                                                  <  2:10133199/140‑1 (MQ=255)
      atTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTc                                                                                                                                  <  1:6525869/140‑1 (MQ=255)
       ttCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAATCGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTcg                                                                                                                                 >  1:3745684/1‑140 (MQ=255)
         cAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTcgcg                                                                                                                               <  1:3349418/140‑1 (MQ=255)
             cGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCg                                                                                                                           >  2:5309202/1‑140 (MQ=255)
             cGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCg                                                                                                                           >  1:616966/1‑140 (MQ=255)
               ttGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGAt                                                                                                                         >  2:6510564/1‑140 (MQ=255)
               ttGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGAt                                                                                                                         >  1:7228853/1‑140 (MQ=255)
                    ggaagtggaaAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAgcgc                                                                                                                    >  2:10331825/1‑140 (MQ=255)
                      aagtggaaAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAgcgcgc                                                                                                                  <  1:7919816/140‑1 (MQ=255)
                           gaaAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGcc                                                                                                             >  1:4289758/1‑140 (MQ=255)
                           gaaAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGcc                                                                                                             <  2:802313/140‑1 (MQ=255)
                            aaaaaGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCACAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCg                                                                                                            >  2:7025857/1‑140 (MQ=255)
                            aaaaaGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCg                                                                                                            >  1:5827007/1‑140 (MQ=255)
                            aaaaaGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCg                                                                                                            <  1:10280327/140‑1 (MQ=255)
                             aaaaGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGa                                                                                                           >  1:90455/1‑140 (MQ=255)
                                   tgagaatCTGGAGGCGCTGCAGAAAACGGTCGTCAACAAAACACAAGACGCCCGTCACATAGGCATCACGCGCAAAACGATAATGGCAAAACTGTCGCGTTAGTACTGCTCGCGTTCGATAGCGCGCCAGCCGATATCtt                                                                                                     >  2:5961414/7‑140 (MQ=255)
                                      gATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTcc                                                                                                  >  2:6303074/1‑140 (MQ=255)
                                      gATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTcc                                                                                                  <  1:2497913/140‑1 (MQ=255)
                                       aTTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCg                                                                                                 <  2:3407547/140‑1 (MQ=255)
                                            ggcggcGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGa                                                                                            <  1:10391821/140‑1 (MQ=255)
                                                 cgcTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAg                                                                                       <  1:9324454/140‑1 (MQ=255)
                                                  gcTGGAGAACACGGGCGGCCCCAACACCGAAGCCGCCCGTCAGTTCGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgt                                                                                      <  1:8666544/140‑1 (MQ=255)
                                                  gcTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgt                                                                                      <  1:5176410/140‑1 (MQ=255)
                                                  gcTGGAGAAAACAGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTTGGGATCACGCGCAAAACGCGATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgt                                                                                      <  2:897077/140‑1 (MQ=255)
                                                    tGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgtcg                                                                                    <  1:7618136/140‑1 (MQ=255)
                                                      gagaAAACGGGCGGCAACAAACCCGAAGCCGCCCGTCAGTGAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgtcgtc                                                                                  <  2:3738436/140‑1 (MQ=255)
                                                      gagaAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgtcgtc                                                                                  <  2:5559312/140‑1 (MQ=255)
                                                      gagaAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgtcgtc                                                                                  <  1:1282035/140‑1 (MQ=255)
                                                      gagaAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCGCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgtcgtc                                                                                  <  2:4306336/140‑1 (MQ=255)
                                                      gagaAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTGAGGGATCACGCGCACAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATCTCTTTCCGGCAGAAGCAgtcgtc                                                                                  <  1:3135855/140‑1 (MQ=255)
                                                       agaAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTcc                                                                                 <  1:6724354/140‑1 (MQ=255)
                                                       agaAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTcc                                                                                 <  1:2770062/140‑1 (MQ=255)
                                                         aaaaCGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAACCGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCa                                                                               <  2:3034227/140‑1 (MQ=255)
                                                          acaCGGGCGGCAACAAAACCGAAGCCGCCCGGCAGTGAGGGCGCACGCGCAACACGCGATGGGCAAAACTGGCGCGGTAGTGCTGCTCGCGTTCGAGAGCGCGCCAGCCGAGCTCTTTCCGGCAGAAGCAGTCGTCCCAg                                                                              <  2:4965489/138‑1 (MQ=255)
                                                             cGGGCGGAAACAATACCGAAGCCGCCCGTGAGTTAGGGATCACGCGCAAAACGCAATTGGCAAAACTGTCGCGTTGGTTCTGCTAGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGTAGCAGTCGTCCCAGTgg                                                                           >  2:878747/1‑140 (MQ=255)
                                                                gcggcAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCTGCAGATATCTTTACGGCAGAAGCGGTCGTCCGAGTGGatc                                                                        >  2:3960101/1‑139 (MQ=255)
                                                                gcggcAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGata                                                                        >  1:48138/1‑140 (MQ=255)
                                                                gcggcAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGata                                                                        <  2:4652045/140‑1 (MQ=255)
                                                                gcggcAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTGCTGCTCGCGTTCGATAGCGCGCCAGCCGATCTCTGTCCGGCAGAAGCAGTCGTCCCAGTGGata                                                                        >  2:1153003/1‑140 (MQ=255)
                                                                 tggcAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGatat                                                                       <  2:219247/139‑1 (MQ=255)
                                                                   gcAACATAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCTAAACGCTATTGGCAAATCTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGTATATCg                                                                     <  2:9730109/140‑1 (MQ=255)
                                                                   gcAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCg                                                                     <  2:5869393/140‑1 (MQ=255)
                                                                   gcAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCg                                                                     <  1:4743077/140‑1 (MQ=255)
                                                                   gcAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCg                                                                     <  1:8243595/140‑1 (MQ=255)
                                                                   gcAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTCCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCg                                                                     <  1:10829004/140‑1 (MQ=255)
                                                                     aacaaAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGt                                                                   <  2:9193520/140‑1 (MQ=255)
                                                                     aacaaAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGt                                                                   <  1:7738193/140‑1 (MQ=255)
                                                                     aacaaAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGt                                                                   <  2:6137816/140‑1 (MQ=255)
                                                                        aaaaCCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCAt                                                                <  1:4975617/140‑1 (MQ=255)
                                                                          acccGAAGCCGCCCGTCAGTTACGGAACACGCGCACACCGCGATTGGCAACACTGTCGCGTTAGGTCGGCACGCGTTCGAAAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGAGGATATCGGTCATTa                                                              <  2:1166357/138‑1 (MQ=255)
                                                                          aaCCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTa                                                              <  1:2995147/140‑1 (MQ=255)
                                                                            ccGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAg                                                            >  2:3217589/1‑140 (MQ=255)
                                                                                     cccGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGgcg                                                   <  2:7415573/140‑1 (MQ=255)
                                                                                      ccGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGgcgc                                                  <  1:1481476/140‑1 (MQ=255)
                                                                                              tAGGGATCACGCGCAAAACGCTATTGGCAACACTGTCGCGTTCGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTgc                                          <  2:6700558/140‑1 (MQ=255)
                                                                                               aGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTgcg                                         >  2:6964159/1‑140 (MQ=255)
                                                                                                   aTCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTc                                     >  2:2239072/1‑140 (MQ=255)
                                                                                                    tCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTACGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCt                                    >  2:9429708/1‑140 (MQ=255)
                                                                                                     cACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTATCCGGCAGAAGCAGACGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTg                                   >  1:10985841/1‑140 (MQ=255)
                                                                                                      aCGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGc                                  >  1:11168757/1‑140 (MQ=255)
                                                                                                       cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGcc                                 <  1:9598266/140‑1 (MQ=255)
                                                                                                       cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGcc                                 >  1:4039101/1‑140 (MQ=255)
                                                                                                       cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGcc                                 >  2:5064930/1‑140 (MQ=255)
                                                                                                       cgcgCAAAAAGCTATTTGCAAAACTGTCGCGTGAGTTCTGCTCGCGTTCTATAGCGCGCCAGCCGAAATCTTTCAGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGAATAGGCGCGTTTCTGCGCTTCTGcc                                 >  2:7735864/1‑140 (MQ=255)
                                                                                                        gcgcAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGTCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCa                                <  1:1806808/140‑1 (MQ=255)
                                                                                                        gcgcAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCa                                <  1:2238963/140‑1 (MQ=255)
                                                                                                        gcgcAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCa                                <  2:10676631/140‑1 (MQ=255)
                                                                                                        gcgcAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCa                                <  1:8883821/140‑1 (MQ=255)
                                                                                                        gcgcAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCa                                <  1:10539488/140‑1 (MQ=255)
                                                                                                            aaaaCGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGt                            <  2:9933080/140‑1 (MQ=255)
                                                                                                                  cTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGAcc                      <  1:8473014/140‑1 (MQ=255)
                                                                                                                  cTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCAGAGGCGCGTTTCTGCGCTTCTGCCACGGTATGAcc                      >  2:4587033/1‑140 (MQ=255)
                                                                                                                     atGGCAAAACCGTCGCGTTAGTTCTGCTCGCGTTCGTTAGCGCGGCAGCCGTTTACTTTCCGGCATCAGCAGTCGTCCCAGTGGATATCGGTCATTAACGCATACGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAg                   <  1:1008093/139‑1 (MQ=255)
                                                                                                                      tGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCa                                <  2:1448778/126‑1 (MQ=255)
                                                                                                                       ggCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAgcg                 <  1:989586/140‑1 (MQ=255)
                                                                                                                       ggCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAgcg                 <  1:4465230/140‑1 (MQ=255)
                                                                                                                       ggCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAgcg                 <  1:5427892/140‑1 (MQ=255)
                                                                                                                       ggCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAgcg                 <  1:648669/140‑1 (MQ=255)
                                                                                                                       ggCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAgcg                 <  2:2613167/140‑1 (MQ=255)
                                                                                                                       ggCAAAACTGTCGCGTTAGTTCTGCTCGCGTACGCTAGCGCGCCAGCCGATATCTTGCCGGCAGAAGCAGACGTCCCACTGGATATCGGTCATGAAGGCATCGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAgcg                 <  2:7806264/140‑1 (MQ=255)
                                                                                                                       ggCAAAACTGTCGCGTTAGTTCTGCTCGCGATCGATATCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAgcg                 <  2:10827277/140‑1 (MQ=255)
                                                                                                                          aaaaCTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCgg              <  2:1824189/140‑1 (MQ=255)
                                                                                                                           aaaCTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGt             <  1:5659411/140‑1 (MQ=255)
                                                                                                                             aCTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGa           <  2:8314826/140‑1 (MQ=255)
                                                                                                                               tGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCTCTTCTGCCACGGTATGACCCAGCGCGGTGACg         <  2:5466155/140‑1 (MQ=255)
                                                                                                                               tGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACg         <  1:210885/140‑1 (MQ=255)
                                                                                                                                 tCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGca       <  2:11003225/140‑1 (MQ=255)
                                                                                                                                 tCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGca       <  1:11401526/140‑1 (MQ=255)
                                                                                                                                 tCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGca       <  1:232984/140‑1 (MQ=255)
                                                                                                                                 tCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGca       <  2:5747874/140‑1 (MQ=255)
                                                                                                                                 tCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGAGATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGca       <  2:9593438/140‑1 (MQ=255)
                                                                                                                                 tCGCGGTAGTTCTGCTCGCGTTCGATCGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGca       <  2:11331643/140‑1 (MQ=255)
                                                                                                                                  cgcgTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGcac      >  2:2155737/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
CAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGCACAGTA  >  NZ_CP025268/4206430‑4206703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑