BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,728,808	(C)_10→8	pseudogene (1254‑1255/1457 nt)	xylB ←	xylulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,728,800	0	C	.	93.0%	536.3 / 29.4	129	pseudogene (1263/1457 nt)	xylB	xylulokinase
Reads supporting (aligned to +/- strand): ref base C (9/0); new base . (59/61); total (68/61)
Fisher's exact test for biased strand distribution p-value = 3.25e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NZ_CP025268	3,728,801	0	C	.	91.7%	443.8 / 53.4	129	pseudogene (1262/1457 nt)	xylB	xylulokinase
Reads supporting (aligned to +/- strand): ref base C (2/0); major base . (51/59); minor base G (8/2); total (68/61)
Fisher's exact test for biased strand distribution p-value = 5.17e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

GGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCC  >  NZ_CP025268/3728661‑3728932

||

ggCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCATc                                                                                                                                      >  1:4427972/1‑140 (MQ=255)

  cTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccc                                                                                                                                    <  2:4249996/140‑1 (MQ=255)

  cTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCAAGTGCTGGCCCCACATccc                                                                                                                                    <  1:2149473/140‑1 (MQ=255)

     aTAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATcccccc                                                                                                                                 >  1:3317693/1‑140 (MQ=255)

        aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccg                                                                                                                              <  1:5849591/140‑2 (MQ=255)

         gCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgg                                                                                                                             >  1:7112870/1‑138 (MQ=255)

          cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgta                                                                                                                            <  1:9545046/140‑4 (MQ=255)

          cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgta                                                                                                                            <  1:8104552/140‑4 (MQ=255)

          cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgta                                                                                                                            <  1:7842281/140‑4 (MQ=255)

          cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAAGTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgta                                                                                                                            <  2:4131588/140‑4 (MQ=255)

             cATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATcgccccccggacgg                                                                                                                         >  2:4579555/1‑134 (MQ=255)

                     tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTACTCGAg                                                                                                               <  1:10185863/140‑1 (MQ=255)

                     tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAg                                                                                                               <  2:8658763/140‑1 (MQ=255)

                     tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAg                                                                                                               <  1:10184153/140‑1 (MQ=255)

                     tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAg                                                                                                               <  1:1847715/140‑1 (MQ=255)

                       cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CGCCCCCCCCGTACGGTGATCGAggt                                                                                                             >  1:8709289/1‑138 (MQ=255)

                        gcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgctg                                                                                                            <  1:6451575/140‑1 (MQ=255)

                        gcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTAAATGAGCGATTTCTCTGGATTCGCCGCGATCTTCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgctg                                                                                                            <  2:6633953/140‑1 (MQ=255)

                            cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCAT‑‑CCCCCCCCGTACGGGACTCGAgctgcggg                                                                                                        >  2:6797638/1‑137 (MQ=255)

                               gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CACCCCCCGTACGGTAATCGAGCTGCTGACCg                                                                                                     >  2:8953683/1‑140 (MQ=255)

                                    tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTGCGGGACTCGAGCTGCTGACCGcgggt                                                                                                >  2:7904141/1‑136 (MQ=255)

                                    tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGat                                                                                                >  1:1026550/1‑140 (MQ=255)

                                    tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CACCCACCGTACGGTAATCGAGCTGCTTACCgatgat                                                                                                >  2:921949/1‑140 (MQ=255)

                                      cGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTGCGGGAGGCGAGCTGCTGACCGCTGagat                                                                                              >  2:7225258/1‑140 (MQ=255)

                                      cGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAAGCGAGCTGCTGACCGCTGatat                                                                                              >  2:7224722/1‑140 (MQ=255)

                                         cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGc                                                                                          <  1:190478/140‑1 (MQ=255)

                                         cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGTTCGGTGGTCGAGCTGCGGTCCGCTGATATCCg                                                                                           >  2:2072404/1‑140 (MQ=255)

                                          tGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGc                                                                                          <  1:8487955/140‑1 (MQ=255)

                                             tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGAATCGAGGTGCTGGCCGCTGTGAGCCGCCAg                                                                                       >  2:2596140/1‑140 (MQ=255)

                                                 aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CGCCCCCCGTACGGTAATCGCGCTGCGGACCGCTGGTATCCGCGAGCagc                                                                                   >  1:10526763/1‑138 (MQ=255)

                                                 aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATc                                                                                   >  2:3054585/1‑140 (MQ=255)

                                                 aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCAGCTGACCGCTGATATCCGCCAGCagc                                                                                   >  2:8556716/1‑138 (MQ=255)

                                                 aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GGCCCCCCCGTTCGGGAATCGGGCTGCGGGCCGCTGATATCCGCCAGCagc                                                                                   >  2:2170177/1‑138 (MQ=255)

                                                  cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                  <  1:6078751/140‑1 (MQ=255)

                                                  cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                  <  1:2687315/140‑1 (MQ=255)

                                                  cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                  <  1:2808731/140‑1 (MQ=255)

                                                  cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                  <  2:10909367/140‑1 (MQ=255)

                                                  cGGTAGTAGCGGCAACAATTCAATGAGCGCTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                  <  1:11034813/140‑1 (MQ=255)

                                                    gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                <  1:4874191/140‑1 (MQ=255)

                                                    gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                <  1:650863/140‑1 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                >  2:1957223/1‑138 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                              <  2:5852633/140‑1 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                              <  2:9628170/140‑1 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGGAAGCGTGCTGCTGACCGCTGATATCCGCCAGCCTCTGACg                                                                              >  1:3568361/1‑140 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCACCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                              >  1:9692107/1‑140 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCACCCCCGAACGGTACTCGAGCTGCTGACCGCTGATATCCGCCCGCATCGGACg                                                                              >  2:8548759/1‑140 (MQ=255)

                                                       gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                             <  2:3239222/140‑1 (MQ=255)

                                                       gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                             <  1:11272764/140‑1 (MQ=255)

                                                        ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                                                            <  1:9234680/140‑1 (MQ=255)

                                                          gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAg                                                                          <  2:4463525/140‑1 (MQ=255)

                                                          gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAg                                                                          <  2:8814176/140‑1 (MQ=255)

                                                           cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGGACGGTCATCGAGCGGCGGGCCGGGGGTTGCCGCCCGCCGCAGACGCCAgg                                                                         >  1:11306810/1‑139 (MQ=255)

                                                           cGGCAACAATTCAATGAGCGATTTCTCTGGATACGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTAATCGAGCTGCTGTCGGCTGATATCCGCCCGCATCTGACGCCAgg                                                                         >  2:9126091/1‑139 (MQ=255)

                                                            ggCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTa                                                                        <  1:1489510/140‑1 (MQ=255)

                                                              caacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACt                                                                      <  2:3796985/140‑1 (MQ=255)

                                                               aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTc                                                                     <  2:3635800/140‑1 (MQ=255)

                                                                   aTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTa                                                                 <  1:5269831/140‑1 (MQ=255)

                                                                    ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAc                                                                <  2:3357569/140‑1 (MQ=255)

                                                                    ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTAATCGAGCTGCGGACCGCGGTTGTCGGCCAGCCTCTGACGCCCGGACTCGCTAc                                                                >  2:452424/1‑140 (MQ=255)

                                                                          gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                          <  2:1780853/140‑1 (MQ=255)

                                                                            gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CGCCCCCCCCGTACGGGACTCGAGCTGCTGACCGCTGAGATCCGCCAGCAGCTGTCGCCCGTCCGCACTACGcgcgccg                                                        >  2:10960809/1‑140 (MQ=255)

                                                                              gATTTCTCTGGATTCGCCGCGTTCTGCGCCAGCCTTGCTGCGCCCTGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGTTATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgcc                                                      <  2:8567096/140‑1 (MQ=255)

                                                                              gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTAATCGAGCTGCGGCCCGCTGGTCTCCGCCCGCAGCTGGCGCCAGTCCTCACTACGcgccccgcc                                                      >  1:6237434/1‑140 (MQ=255)

                                                                                    tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                <  1:1579612/140‑1 (MQ=255)

                                                                                    tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                <  1:310891/140‑1 (MQ=255)

                                                                                    tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                <  1:8341064/140‑1 (MQ=255)

                                                                                    tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                <  1:737475/140‑1 (MQ=255)

                                                                                     ctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTTATCGTGCTGCTGGCCGCTGATATCCGCCAGCAGCGGACGCCGGTTCTCACGACGCGCCCCGcccccgggg                                               >  1:3282659/1‑136 (MQ=255)

                                                                                      tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCC‑‑CTCCCCCCCCGGGCGGGAAGCGAGGTGCTGTCCGCGGATTTGCGCCAGCAGCTGTCGCCCGTACTCACGACGCGCCCCGcccccgttca                                              >  2:2439558/1‑135 (MQ=255)

                                                                                          ttCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTCCTCGAGGTGCTGACCGCTGATCTCCGCCAGCATCTGACGCCAGTACTCTCTACGCGCCCCGCCCCCAATCAACGt                                          >  2:8133653/1‑140 (MQ=255)

                                                                                             gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCAT‑‑CCCCCCCCGTGCGGTGTTCGGGCTGGTGACCGGTGTGATCCGGCAGCTTCGGTCGCCAGGACGCGCTTCGCGCCCCGCCCCCAATCAACGTAac                                       >  2:2902499/1‑140 (MQ=255)

                                                                                              ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGTCCGCGGTTATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAGTCTACGTGaca                                      >  1:4356914/1‑140 (MQ=255)

                                                                                              ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGGAATCGAGCTGCGGGCCGCTGATATCCGCCAGCATCTGACGCCCGGACTCGCGACGCGCCCCGCGCCCACTCAACGGAaca                                      >  1:6923542/1‑140 (MQ=255)

                                                                                                 cgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACTCGCCCCGCCCCCAATCAACGTAACACtt                                   <  1:1535394/140‑1 (MQ=255)

                                                                                                 cgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACtt                                   <  1:6204475/140‑1 (MQ=255)

                                                                                                   acaggcgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                 <  1:5731413/136‑1 (MQ=255)

                                                                                                    tCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGGCCTCGAGCTGCTGACCGCGGCTGTCCGCCAGCCTCTGACGCCCGGACTCACTACGCGCCCCGCCCCCACGCAACGTCACACGTTGc                                >  1:8046080/1‑140 (MQ=255)

                                                                                                        cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACGT‑‑CCCCCCCCGGGCGGTCCTCGGGCTGCTGGCCGCGGCTGTGCGGCAGCAGCTGTCGCGCGGAGTCAGTACGCGCCCGGCCCCCGAGCACCGGAACGCTGTGCGGtt                            >  2:5503445/1‑140 (MQ=255)

                                                                                                           cAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTCCGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACTCAACGTAACACTTTGCGGTTTaa                         <  2:5509494/140‑1 (MQ=255)

                                                                                                                   ctgcGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAg                 <  2:3013868/140‑1 (MQ=255)

                                                                                                                     gcgcCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGc               <  1:5856888/140‑1 (MQ=255)

                                                                                                                     gcgcCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGc               <  1:5788315/140‑1 (MQ=255)

                                                                                                                       gcCCAGTGCTGGCCCCACAT‑‑GCCCCCCCGGACGGTAATCGGGCTGCTGACCGCTGAGATCCGCCAGCCTCTGACGCCAGGCCGCACTACGCGCCCCGCCCCGCATCCACGGAAGACTGTGCGGGTTTATAGCGCAGGCAt             >  1:3035937/1‑140 (MQ=255)

                                                                                                                        cccAGTGCTGGCCCCACTT‑‑CCCCCCCCGTACGGTAATCGAGCTGCGGACCGCTGATATCCGCCAGCATCTGACGCCCGGACTCACTTCGCGCCCCGCCCCCGATCAACGTAACACTTTGCGGGGTAATCCCGCAGGCATg            >  2:863235/1‑140 (MQ=255)

                                                                                                                        cccAGTGCTGGCCCCACAT‑CCCCCCCCCGTGCGGTCATCGGGCTGCTGACCGCGGCGAGCCGCCCGCATCTGACGCCAGTGGTCACTACGCGCCCCGCCCCCAGTCTACGTCACACTTTGCGGGTGAATAACGCAGGcgt             >  2:10334011/1‑138 (MQ=255)

                                                                                                                        cccAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGAGCGCTGATATACGCCAGCATCTGACGCAAGTAATCACTAAGCGCCCCGCCCCCAATCCAAGTAAAACTTTGCGGGTTAATACcgcacgcaag            >  2:3715527/1‑138 (MQ=255)

                                                                                                                        cccAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTCCTCACTTCGCGCCCCGCCCCCAATCAACGAAACACTTTGCGGTTTAAGACCGCGGGCATg            >  2:7691776/1‑140 (MQ=255)

                                                                                                                        cccAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATg            >  2:10243839/1‑140 (MQ=255)

                                                                                                                        cccAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCGGCTGACCGCTGTTATCCGCCAGCATCTGGCGGCGGGTCTCGCTACGCGCCCCGCCCCCAATCAACGGATCCCTTTGCGGGGTAATTCCGCAGGCATg            >  2:1373614/1‑140 (MQ=255)

                                                                                                                         ccAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTCATCGAGCTGCTGACCGCTGATATCCGCCAGCAGCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAGCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGc           >  1:9034524/1‑140 (MQ=255)

                                                                                                                         ccAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAGCTGACGCCCGGACTCACTACGCGCCCCGCCCCCAATCAACGTCACACTTTGCGGTTTAATACCGCAGGcgggc           >  1:9034535/1‑136 (MQ=255)

                                                                                                                           aGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTTATCGAGCTGCTGACCGCTGTTATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCGCCAAGCAACGGAACACTTTGCGGTTTAATTCCGCAGGCCTGcgc         >  1:2054884/1‑138 (MQ=255)

                                                                                                                           aGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGGAATGGTGCTGCTGTCCGCTGATCTCCGCCAGCGTCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACCCGTTGCGGGTTAATACCGCCGGCATGCAc         >  1:1072570/1‑140 (MQ=255)

                                                                                                                            gTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACg        <  1:1674635/140‑1 (MQ=255)

                                                                                                                             tGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGTCCGCGGATATCCGCCCGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACGTTGCGGTTTTAGACCGCGGGCGTGCACGa       >  2:9719143/1‑140 (MQ=255)

                                                                                                                             tGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCG‑CCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGAc      >  2:7592667/1‑140 (MQ=255)

                                                                                                                             tGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCG‑CCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGAc      >  2:3855477/1‑140 (MQ=255)

                                                                                                                               cTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGATGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCTCCCCCAATCAACGTAACACTTTTCGGTTTAATACCGCAGGCATGCACGACa     <  2:2826156/140‑1 (MQ=255)

                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGCCCGCGGATATCCGCCAGCATCTGACGCCAGTACTCACTCCGCGCCCCGCCCCCAAGCAACGTAACACTTTGCGGTTTAAGACCGCAGGCCTGCACGACCTcc  >  1:1561712/1‑140 (MQ=255)

                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGGTAATACCGCAGGCATGCACGACATcc  >  1:11101655/1‑140 (MQ=255)

||

GGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCC  >  NZ_CP025268/3728661‑3728932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`