BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,629,176	(T)_7→8	pseudogene (141/392 nt)	ydeI ←	YdeI family stress tolerance OB fold protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,629,169	1	.	T	95.6%	1159.8 / 38.1	317	pseudogene (148/392 nt)	ydeI	YdeI family stress tolerance OB fold protein
Reads supporting (aligned to +/- strand): ref base . (2/12); new base T (76/227); total (78/239)
Fisher's exact test for biased strand distribution p-value = 5.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 327 total aligned reads displayed.

GCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑T‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACAAT  >  NZ_CP025268/1629034‑1629301

gCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑t‑t‑tt                                                                                                                                   >  2:361562/1‑140 (MQ=255)

gCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑t‑t‑tt                                                                                                                                   <  1:4802419/140‑1 (MQ=255)

 ccGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑t‑t‑ttt                                                                                                                                  >  2:8282000/1‑140 (MQ=255)

    cAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCACTCCTTTGTGAGAAATAAAATTACCGCGTAGTGAAACCCAAGCGCCATCGTGCATTGTTTTTGCGAAATCAACGGTCA‑a‑t‑tttt‑tt                                                                                                                              >  2:5627354/1‑139 (MQ=255)

           aaCGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGcatcat                                                                                                                        <  1:6381240/140‑1 (MQ=255)

           aaCGACATTAATTACACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCACATTACCGCGTAGTGAAACCGAAGCGCCCTCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGcatcat                                                                                                                        <  2:10791639/140‑1 (MQ=255)

            aCGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCatcatc                                                                                                                       <  1:10579213/140‑1 (MQ=255)

               aCATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGt                                                                                                                    <  1:1763373/140‑1 (MQ=255)

                 aTTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTc                                                                                                                  >  2:4632667/1‑140 (MQ=255)

                 aTTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTc                                                                                                                  >  2:8341478/1‑140 (MQ=255)

                 aTTAATTTCACAGCTCTTATCGAGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTc                                                                                                                  >  2:711861/1‑140 (MQ=255)

                      tttCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGa                                                                                                             <  1:4632029/140‑1 (MQ=255)

                      tttCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGa                                                                                                             <  1:4236448/140‑1 (MQ=255)

                      tttCACCGCTCTTATCGCGAAAAACGNAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGa                                                                                                             <  1:6790152/140‑1 (MQ=255)

                          aCCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGc                                                                                                         >  1:3645461/1‑140 (MQ=255)

                           ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCACATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGcc                                                                                                        <  2:9671256/140‑1 (MQ=255)

                           ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGcc                                                                                                        <  1:7397184/140‑1 (MQ=255)

                           ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGcc                                                                                                        <  2:5393364/140‑1 (MQ=255)

                           ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGcc                                                                                                        <  1:521245/140‑1 (MQ=255)

                           ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGcc                                                                                                        <  2:1796920/140‑1 (MQ=255)

                           ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGcc                                                                                                        <  1:266343/140‑1 (MQ=255)

                           ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGcc                                                                                                        >  1:2050676/1‑140 (MQ=255)

                           ccGCTCTTATCGCGAAAAACGAAACGGTCCTCTCCTTTGTCAGAAATCAAATTACCGCGTACAGAAACCCAACCGCCAACGTCCATGGTTTTTGCGAAAACAACCGTCA‑TAA‑TTTT‑AGCATCAACGGCACCGCGATAGcc                                                                                                        <  2:4537937/140‑1 (MQ=255)

                            cGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCa                                                                                                       >  1:7810487/1‑140 (MQ=255)

                             gCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCAt                                                                                                      >  1:8067557/1‑140 (MQ=255)

                              ctctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCAtc                                                                                                     <  1:10895743/140‑1 (MQ=255)

                              ctctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCAtc                                                                                                     <  2:3507426/140‑1 (MQ=255)

                              ctctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCAtc                                                                                                     <  1:7044218/140‑1 (MQ=255)

                                ctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCAtctt                                                                                                   <  2:1468838/140‑1 (MQ=255)

                                   aTCGCGAAACACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTa                                                                                                <  1:1263914/140‑1 (MQ=255)

                                        gAAAAACGTAACGGTCCTCTCCTTTGTGAGAACTCAATTTACCGAGTAGTGAAACCGAAGCGCCATCGTGCATGGTGTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCa                                                                                           >  2:10627015/1‑140 (MQ=255)

                                        gAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCa                                                                                           <  2:5908941/140‑1 (MQ=255)

                                         aaaaaCGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCAt                                                                                          <  2:3222214/140‑1 (MQ=255)

                                         aaaaaCGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCAt                                                                                          <  2:3024744/140‑1 (MQ=255)

                                                   cGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGcggcgg                                                                                <  1:8475548/140‑1 (MQ=255)

                                                   cGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGcggcgg                                                                                <  1:2229459/140‑1 (MQ=255)

                                                     gTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTg                                                                              <  2:11322205/140‑1 (MQ=255)

                                                     gTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTg                                                                              <  1:2398792/140‑1 (MQ=255)

                                                     gTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGACATCGTGAATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTg                                                                              <  2:2146151/140‑1 (MQ=255)

                                                      tCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTa                                                                                                >  1:973570/1‑121 (MQ=255)

                                                      tCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTa                                                                                                <  2:973570/121‑1 (MQ=255)

                                                        ctctCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGgcg                                                                           >  1:9147091/1‑140 (MQ=255)

                                                            ccTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑T‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCa                                                                      <  2:11070979/140‑1 (MQ=255)

                                                               ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                    <  1:5580946/140‑1 (MQ=255)

                                                               ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                    <  1:9914691/140‑1 (MQ=255)

                                                               ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                    <  1:6317808/140‑1 (MQ=255)

                                                               ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                    <  1:11045433/140‑1 (MQ=255)

                                                                tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATcc                                                                   >  2:1885549/1‑140 (MQ=255)

                                                                tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATcc                                                                   >  1:1626361/1‑140 (MQ=255)

                                                                tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATcc                                                                   >  2:6250636/1‑140 (MQ=255)

                                                                       aaTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TTCATCATCTGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTa                                                            >  2:8878269/1‑140 (MQ=255)

                                                                       aaTCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTa                                                            <  2:155529/140‑1 (MQ=255)

                                                                         tCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTaaa                                                          <  1:9614703/140‑1 (MQ=255)

                                                                         tCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTaaa                                                          <  2:8526446/140‑1 (MQ=255)

                                                                         gcAAATTACCGCGTAGTGAAACCTCAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTaaa                                                          <  2:6667055/139‑1 (MQ=255)

                                                                          cAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCACCGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAAc                                                         <  1:7179131/140‑1 (MQ=255)

                                                                                aCCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCt                                                   <  1:4761601/140‑1 (MQ=255)

                                                                                    cgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTc                                               >  1:8170486/1‑140 (MQ=255)

                                                                                    cgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCAATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTc                                               >  1:1605629/1‑140 (MQ=255)

                                                                                      tAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCAt                                             <  1:1976356/140‑1 (MQ=255)

                                                                                       aGTGAAACCGACGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATc                                            <  1:2551612/140‑1 (MQ=255)

                                                                                          gAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGc                                         <  1:9294199/140‑1 (MQ=255)

                                                                                           aaaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGcc                                        <  2:10031850/140‑1 (MQ=255)

                                                                                           aaaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGcc                                        <  1:3572529/140‑1 (MQ=255)

                                                                                            aaCCGAAGCGCCATCGTGCTGGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCa                                       <  2:10363719/140‑1 (MQ=255)

                                                                                            aaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCa                                       <  2:9405880/140‑1 (MQ=255)

                                                                                            aaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCa                                       <  1:9038258/140‑1 (MQ=255)

                                                                                            aaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCa                                       <  2:10489491/140‑1 (MQ=255)

                                                                                            aaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCa                                       <  1:5432982/140‑1 (MQ=255)

                                                                                              ccGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAAc                                     <  1:2883419/140‑1 (MQ=255)

                                                                                                 aaGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCa                                  <  1:6636319/140‑1 (MQ=255)

                                                                                                 aaGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCa                                  <  2:5767331/140‑1 (MQ=255)

                                                                                                   gcgcCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATa                                <  2:2367475/140‑1 (MQ=255)

                                                                                                   gcgcCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATa                                >  1:278067/1‑140 (MQ=255)

                                                                                                   gcgcCATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATa                                >  2:7403280/1‑140 (MQ=255)

                                                                                                   gcgcCATCGTGCATGGTGTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATa                                >  1:3268497/1‑140 (MQ=255)

                                                                                                      ccATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAgg                             <  1:6306069/140‑1 (MQ=255)

                                                                                                      ccATCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAgg                             <  2:4932101/140‑1 (MQ=255)

                                                                                                        aTCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCa                           >  2:7295821/1‑140 (MQ=255)

                                                                                                         tCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCAt                          <  2:9147091/140‑1 (MQ=255)

                                                                                                         tCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCAt                          <  2:7874433/140‑1 (MQ=255)

                                                                                                         tCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCAt                          <  1:3388043/140‑1 (MQ=255)

                                                                                                         tCGTGCATGGTTTTTGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTACCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCAt                          >  1:10438030/1‑140 (MQ=255)

                                                                                                         tCGTGCATGGTTTTTGCGAAATCAACGGTCA‑T‑T‑TTTTGTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCAt                          <  2:4211740/140‑1 (MQ=255)

                                                                                                         tCGGGCATGGTTTTTGCGAAATCAACGGTCA‑T‑TGTTTG‑TGCCTCATCGGTTCCGCGAGAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCAt                          <  2:7996976/140‑1 (MQ=255)

                                                                                                                   tttttGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                <  2:8049402/140‑1 (MQ=255)

                                                                                                                   tttttGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                <  2:2609717/140‑1 (MQ=255)

                                                                                                                   tttttGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                <  1:3161025/140‑1 (MQ=255)

                                                                                                                   tttttGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                <  1:5279587/140‑1 (MQ=255)

                                                                                                                   tttttGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                <  2:4730010/140‑1 (MQ=255)

                                                                                                                   tttttGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                <  2:520267/140‑1 (MQ=255)

                                                                                                                   tttttGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTAGTGCATGAGGCGGCGGTGGCGCTGCATCCTGATTTAACCCGCCCTGGTCATCTGCCAACGCATAAGGCATGACTAGaaaa                <  1:11341499/140‑1 (MQ=255)

                                                                                                                   tttttGCGAAATCAACGGTCA‑T‑T‑TTTTGTGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa                <  1:10740244/140‑1 (MQ=255)

                                                                                                                     tttGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCACCGCATAAGGCATGACAAGAAAACt              <  1:7999473/140‑1 (MQ=255)

                                                                                                                     tttGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACt              <  1:590648/140‑1 (MQ=255)

                                                                                                                     tttGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACt              <  1:5706582/140‑1 (MQ=255)

                                                                                                                     tttGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACt              <  1:4053130/140‑1 (MQ=255)

                                                                                                                      ttGCGAAATCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACtt             >  1:4966110/1‑140 (MQ=255)

                                                                                                                          gAAATCAACGGACATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTa         <  1:6843760/140‑1 (MQ=255)

                                                                                                                             aTCAACGGTCATT‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATa      >  1:10090346/1‑140 (MQ=255)

GCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑T‑T‑TTTT‑TGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACAAT  >  NZ_CP025268/1629034‑1629301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`