Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 1,826,683 (A)7→8 intergenic (‑247/+51) chbB ← / ← osmE PTS N,N'‑diacetylchitobiose transporter subunit IIB/osmotically‑inducible lipoprotein OsmE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252681,826,6761.A93.8% 2694.5 / 147.8 754intergenic (‑240/+58)chbB/osmEPTS N,N'‑diacetylchitobiose transporter subunit IIB/osmotically‑inducible lipoprotein OsmE
Reads supporting (aligned to +/- strand):  ref base . (36/11);  new base A (481/224);  total (519/235)
Fisher's exact test for biased strand distribution p-value = 2.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 765 total aligned reads displayed.

GGCTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CC‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCAT  >  NZ_CP025268/1826540‑1826810
                                                                                                                                          |                                                                                                                                          
       aGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CC‑a‑aa‑aa‑aaacc                                                                                                                               <  1:10968219/140‑4 (MQ=255)
        ggAGTATACGGTATAGACCTGAGCAATAGTATTTATTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CC‑a‑aa‑aa‑aaaccg                                                                                                                              <  2:6154165/140‑5 (MQ=255)
                   tataGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATACTTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGcc                                                                                                                   >  2:39012/1‑140 (MQ=255)
                   tataGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGcc                                                                                                                   >  2:5320449/1‑140 (MQ=255)
                    cgaGACCTGAGCACTAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATGCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑TCGGCGCCATGGCCg                                                                                                                  >  2:9395905/3‑140 (MQ=255)
                    ataGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGTAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCg                                                                                                                  >  2:1473541/1‑140 (MQ=255)
                      aGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCGA‑AA‑AA‑AA‑CCGGCGCCATGGCCGGt                                                                                                                >  1:9887294/1‑140 (MQ=255)
                      aGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGt                                                                                                                >  1:6762948/1‑140 (MQ=255)
                      aGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGt                                                                                                                >  2:11181395/1‑140 (MQ=255)
                        aCCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGttt                                                                                                              >  1:4392985/1‑140 (MQ=255)
                        aCCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGttt                                                                                                              >  1:8864722/1‑140 (MQ=255)
                             aGCAATAGTCTTTCTTTGTCCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCATGACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCTC‑AT‑AA‑AA‑AGGGCGCAATGGCCGGTGTCCgtt                                                                                                         >  2:8360073/1‑140 (MQ=255)
                             aGCAATAGTATTTCTTTGACCTTATAACTTGTTTCTCCTTGACCTGTGGTTATGACCCTCTGCACCTTTCGGGCAAATAATTCGCGAGGATAATGACGAACTCAGG‑CC‑A‑AA‑AA‑AATGCGGCGCAATGGCCGGTTTCGgtg                                                                                                         >  1:10580704/1‑139 (MQ=255)
                             aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCTGGCAAATAATTAGCTAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGTCGCAATGGCCGGTTTCAgtt                                                                                                         >  2:3482456/1‑140 (MQ=255)
                             aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGTTCATTAAGAACTCAGG‑CC‑A‑AA‑AAGAA‑CCGGCGCAATGGCCGGGTTCCgtt                                                                                                         >  1:11164288/1‑140 (MQ=255)
                             aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGTTAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtg                                                                                                         >  2:7663404/1‑139 (MQ=255)
                             aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCACTGGCCGGTTTCCgtt                                                                                                         >  2:655513/1‑140 (MQ=255)
                             aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:5658692/1‑140 (MQ=255)
                             aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:2662170/1‑140 (MQ=255)
                             aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:6240724/1‑140 (MQ=255)
                             aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:5949482/1‑140 (MQ=255)
                             aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:7340352/1‑140 (MQ=255)
                             aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:8163840/1‑140 (MQ=255)
                             aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:8389576/1‑140 (MQ=255)
                             aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:4290799/1‑140 (MQ=255)
                             aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:10433567/1‑140 (MQ=255)
                             aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:1070810/1‑140 (MQ=255)
                             aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:1006090/1‑140 (MQ=255)
                             aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:3343406/1‑140 (MQ=255)
                             aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:10452633/1‑140 (MQ=255)
                             aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:4140208/1‑140 (MQ=255)
                             aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:7995190/1‑140 (MQ=255)
                             aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:8197788/1‑140 (MQ=255)
                             aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:3162682/1‑140 (MQ=255)
                             aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:5041561/1‑140 (MQ=255)
                             aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCAgtg                                                                                                         >  2:10118856/1‑139 (MQ=255)
                             aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CC‑ATAA‑AT‑AA‑CCGGCGCAATGGCCGGTGTCCgtg                                                                                                         >  1:9254131/1‑139 (MQ=255)
                             aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACACTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:4903808/1‑140 (MQ=255)
                             aGCAATAGTATTTCTTTGACCTTAGAAATTGTTTCTCCTTGACCGGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCggt                                                                                                         >  2:5078489/1‑138 (MQ=255)
                             aGCAATAGTATATCTTGGACCTTCTACAGTGTTTCTCCATGACCTGTGGTTATGACCATCTTCACATTTCGGGCAAATAATTCGGGAAGAAAAGTAAGAACTGAGG‑CC‑A‑AACTA‑TA‑GCGGCGCATTGGCCGGTATCGgtg                                                                                                         >  2:1671813/1‑139 (MQ=255)
                                  tAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTAc                                                                                                    >  1:6955161/1‑140 (MQ=255)
                                  tAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTAc                                                                                                    >  1:5169081/1‑140 (MQ=255)
                                  tAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTGGTTAc                                                                                                    >  1:7077911/1‑140 (MQ=255)
                                  tAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑ACGGCGCAATGGCCGGTTTCCGTGGTTAc                                                                                                    >  1:1660760/1‑140 (MQ=255)
                                       tttCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAg                                                                                               >  2:1225356/1‑140 (MQ=255)
                                       tttCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAg                                                                                               >  2:7511668/1‑140 (MQ=255)
                                       tttCTTTGACCTTATAAATTGTTTCTCATTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAAATCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTACCGATGTTACTCAAg                                                                                               >  2:8928648/1‑140 (MQ=255)
                                            ttGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATTGCCGGTTTCCGTTGTTACTAAAGCTTTc                                                                                          >  2:6333421/1‑140 (MQ=255)
                                             tGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCa                                                                                         >  2:7162889/1‑140 (MQ=255)
                                                ccTTATCAATTGTATCTCCTTGACCTGTGGTTAAGACCCTCTTCACATTTCGGGCAAATAACTCGCGAAGATAATTAACAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGAc                                                                                      <  1:9308056/140‑1 (MQ=255)
                                                ccTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGAc                                                                                      <  1:8494415/140‑1 (MQ=255)
                                                   tataAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGaa                                                                                   >  1:1803384/1‑140 (MQ=255)
                                                    ataAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAAt                                                                                  >  1:1261248/1‑140 (MQ=255)
                                                           gTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTAc                                                                           >  2:4104959/1‑140 (MQ=255)
                                                              tctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTc                                                                        >  1:9609472/1‑140 (MQ=255)
                                                              tctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTc                                                                        >  1:2179357/1‑140 (MQ=255)
                                                                 ccTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     <  2:283610/140‑1 (MQ=255)
                                                                 ccTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     <  2:6059836/140‑1 (MQ=255)
                                                                 ccTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     <  1:3025192/140‑1 (MQ=255)
                                                                   ttGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGc                                                                   <  1:15154/140‑1 (MQ=255)
                                                                      aCCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCtg                                                                <  1:5472867/140‑1 (MQ=255)
                                                                           tgtttATGACACTCTTCACATTTCGGGAAAATAATTCTCGAAGATAATTAAGAACTAAGGACA‑A‑AA‑AA‑AA‑CCGGCGAAATGGACGTTTTACGTTTTTACTAAAGATTTCAGACGAATTGATTACTTCGCAGCCTGTGGag                                                           >  2:195184/4‑139 (MQ=255)
                                                                           tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGTTTACTTCGCAGCCTGTGGag                                                           >  1:2644285/1‑139 (MQ=255)
                                                                           tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTggct                                                           >  1:6574646/1‑138 (MQ=255)
                                                                           tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt                                                           >  1:6150366/1‑140 (MQ=255)
                                                                             gTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCa                                                         <  2:2991951/140‑1 (MQ=255)
                                                                                aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtg                                                      >  2:4182369/1‑140 (MQ=255)
                                                                                  gACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                    <  2:6452249/140‑1 (MQ=255)
                                                                                     ccTCTTCACCTTTCGGGCAAATAATTTGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTTGATCAGTGTCGTa                                                 <  1:5935743/140‑1 (MQ=255)
                                                                                     ccTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGACTTGATTACTTCGCAGCCTGTGGATCAGTGTCGTa                                                 >  1:7795112/1‑140 (MQ=255)
                                                                                     ccTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTa                                                 >  1:3614911/1‑140 (MQ=255)
                                                                                       tctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTAtt                                               <  1:757450/140‑1 (MQ=255)
                                                                                       tctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTAtt                                               >  1:4878332/1‑140 (MQ=255)
                                                                                       tctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTAtt                                               <  1:10780277/140‑1 (MQ=255)
                                                                                        ctTCACATTTCGGGCAAGTAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTc                                              >  1:6345122/1‑140 (MQ=255)
                                                                                        ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTc                                              >  1:8577803/1‑140 (MQ=255)
                                                                                        ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTc                                              >  2:3697347/1‑140 (MQ=255)
                                                                                        ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTc                                              >  1:9532223/1‑140 (MQ=255)
                                                                                        ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTc                                              >  1:4494537/1‑140 (MQ=255)
                                                                                            acaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTGACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTAGTCAGca                                          <  2:567797/140‑1 (MQ=255)
                                                                                            acaTTTCGGGCAAAAAATTCGCGAAGATAAGTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                          <  2:4530163/140‑1 (MQ=255)
                                                                                              aTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGcaca                                        >  2:3251724/1‑140 (MQ=255)
                                                                                               tttCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                          <  1:4684152/137‑1 (MQ=255)
                                                                                                ttCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAgg                                      >  1:9075183/1‑140 (MQ=255)
                                                                                                 tCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGt                                     <  1:7856503/140‑1 (MQ=255)
                                                                                                 tCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGt                                     <  1:3761158/140‑1 (MQ=255)
                                                                                                  cGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTc                                    >  1:241080/1‑140 (MQ=255)
                                                                                                        aataatTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGCTCAGTGTCGTATTCAGCACAGGTCTGATaa                              >  2:9260974/1‑140 (MQ=255)
                                                                                                        aataatTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATaa                              >  1:8698040/1‑140 (MQ=255)
                                                                                                           aatTCGCGAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCg                           <  1:3981258/140‑1 (MQ=255)
                                                                                                                gcgAAGATAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGtt                      >  2:6874052/1‑140 (MQ=255)
                                                                                                                      aTAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGAc                <  2:10939566/140‑1 (MQ=255)
                                                                                                                      aTAATTAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGAc                <  2:9606048/140‑1 (MQ=255)
                                                                                                                           tAAGAACTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTc           <  1:341019/140‑1 (MQ=255)
                                                                                                                           tAAGAACTCACG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGTTTTCCGTTGTTACTCAACCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTc           <  2:9160300/140‑1 (MQ=255)
                                                                                                                               aaCTCAGT‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCATGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGt       <  1:10249363/140‑1 (MQ=255)
                                                                                                                                aCTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCTAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTa      >  2:8662155/1‑140 (MQ=255)
                                                                                                                                aCTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTa      >  2:2108800/1‑140 (MQ=255)
                                                                                                                                aCTCAGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTa      <  2:9906772/140‑1 (MQ=255)
                                                                                                                                    aGG‑CCAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTatcat  >  1:2898923/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                          
GGCTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGG‑CC‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCAT  >  NZ_CP025268/1826540‑1826810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑