BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,873,836	(T)_5→6	pseudogene (67/1283 nt)	yeaH →	YeaH/YhbH family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,873,831	1	.	T	96.0%	2101.1 / 68.5	600	pseudogene (62/1283 nt)	yeaH	YeaH/YhbH family protein
Reads supporting (aligned to +/- strand): ref base . (13/11); new base T (372/200); total (386/214)
Fisher's exact test for biased strand distribution p-value = 2.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 688 total aligned reads displayed.

ACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATC  >  NZ_CP025268/1873697‑1873964

aCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGTCCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tttt‑t                                                                                                                                   >  1:2286519/1‑140 (MQ=255)

aCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tttt‑t                                                                                                                                   >  1:5578022/1‑140 (MQ=255)

 cAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tttt‑tt                                                                                                                                  >  1:7514887/1‑139 (MQ=255)

    cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTCCGGGGGGCATATGACCTGGTTTATTGACCGGCGGCTGAACGGCAAAAACAAAAGCAGGGTGAATCGCCAGCG‑tttt‑ttacg                                                                                                                               >  2:5136648/1‑136 (MQ=255)

    cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tttt‑ttacg                                                                                                                               >  2:9569337/1‑136 (MQ=255)

    cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tttt‑ttacg                                                                                                                               >  1:3805739/1‑136 (MQ=255)

    cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tttt‑ttacg                                                                                                                               >  2:6796087/1‑136 (MQ=255)

    cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tttt‑ttacg                                                                                                                               <  2:2241513/140‑5 (MQ=255)

    cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tttt‑tta                                                                                                                                 <  2:8020412/138‑3 (MQ=255)

            gggCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTtata                                                                                                                       >  2:8140524/1‑140 (MQ=255)

            gggCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTtata                                                                                                                       <  1:2443561/140‑1 (MQ=255)

                  gagaAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAgcgc                                                                                                                 <  1:10679477/140‑1 (MQ=255)

                     aaGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTT‑TGTCGGCGTTATAGAGCGCaag                                                                                                              >  2:7252093/1‑139 (MQ=255)

                        cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTa                                                                                                           >  1:8453454/1‑140 (MQ=255)

                          gCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGACAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTaaa                                                                                                         >  2:8773752/1‑140 (MQ=255)

                              cacaGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGt                                                                                                     <  2:14761/140‑1 (MQ=255)

                              cacaGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGt                                                                                                     <  2:4740147/140‑1 (MQ=255)

                                caGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCg                                                                                                   >  1:584198/1‑140 (MQ=255)

                                     gTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAATATCAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGGTATAAAGCGCAAATTAAACAGTCGAtctc                                                                                              >  1:6858318/1‑140 (MQ=255)

                                      tAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTcc                                                                                             <  2:1951239/140‑1 (MQ=255)

                                      tAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTcc                                                                                             <  1:7210128/140‑1 (MQ=255)

                                         cATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGGTATAAAGCGCAAATTAAACAGTCGATCTCCGAg                                                                                          >  1:10521739/1‑140 (MQ=255)

                                          aTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                         <  1:9314073/140‑1 (MQ=255)

                                           tACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGc                                                                                        >  2:455161/1‑140 (MQ=255)

                                             cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                                      >  2:3941735/1‑140 (MQ=255)

                                             cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                                      >  1:1218847/1‑140 (MQ=255)

                                             cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                                      >  2:327837/1‑140 (MQ=255)

                                              aGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAt                                                                                     >  2:9310152/1‑140 (MQ=255)

                                              aGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAt                                                                                     >  2:7525281/1‑140 (MQ=255)

                                               gTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAtt                                                                                    >  1:10022883/1‑140 (MQ=255)

                                                ttGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAATCAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTATACAGTCGCTCTCCGAGGCCAtga                                                                                   >  2:6339989/1‑138 (MQ=255)

                                                  ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAATGCGCAAATTAAACAGTCGATCTCCGAGGCCATgaat                                                                                 >  1:10884304/1‑140 (MQ=255)

                                                  ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                 >  1:9104774/1‑140 (MQ=255)

                                                  ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                 >  2:3623860/1‑140 (MQ=255)

                                                   gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATACAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaata                                                                                >  2:5445379/1‑140 (MQ=255)

                                                   gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaata                                                                                >  2:5964296/1‑140 (MQ=255)

                                                   gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaata                                                                                >  1:6064024/1‑140 (MQ=255)

                                                     aatggtagtaCGGTGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAACGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTTATAAg                                                                              <  2:1636906/136‑1 (MQ=255)

                                                     aaaTGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAg                                                                              <  2:5819842/140‑1 (MQ=255)

                                                        tGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGt                                                                           <  2:3769421/140‑1 (MQ=255)

                                                         gtagtaCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGtt                                                                          <  1:3309125/140‑1 (MQ=255)

                                                          tagtaCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTc                                                                         <  2:7644421/140‑1 (MQ=255)

                                                             taCGGGGGGCATATGACCTGGTTTATTGACTGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGt                                                                      >  1:7733487/1‑140 (MQ=255)

                                                             taCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGt                                                                      >  1:4447398/1‑140 (MQ=255)

                                                             taCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGt                                                                      >  1:8912710/1‑140 (MQ=255)

                                                             taCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATGAATAAGCGTTCGGt                                                                      >  1:2923692/1‑140 (MQ=255)

                                                               cGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtga                                                                    <  1:1395262/140‑1 (MQ=255)

                                                               cGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtga                                                                    <  1:10094829/140‑1 (MQ=255)

                                                               cGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtga                                                                    <  2:2092535/140‑1 (MQ=255)

                                                               cGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtga                                                                    >  1:4624880/1‑140 (MQ=255)

                                                                  ggggCATATGACCTGGTTTTTTGTCCGGCGGCTGCACGGCAAAAAGAACTGCGTGGTGAATCGGCAGCGTTTTT‑T‑ACGAAGTGATCAAGCGCCATTTAAACAGTCGATCTCCGAGGCCAGTAAAACGCGTTCGGtgacgg                                                                 >  2:6163086/1‑138 (MQ=255)

                                                                  ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACTGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGGTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                 >  2:4914673/1‑140 (MQ=255)

                                                                  ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                 >  1:966916/1‑140 (MQ=255)

                                                                    ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactgac                                                               >  1:5114656/1‑140 (MQ=255)

                                                                       atatGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTc                                                            >  2:4120536/1‑140 (MQ=255)

                                                                            aCCTGGTTTATTGACCGGCGTCTGACCGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAg                                                       <  1:10320654/140‑1 (MQ=255)

                                                                               tGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcgg                                                    >  1:6263064/1‑140 (MQ=255)

                                                                               tGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcgg                                                    >  1:7303920/1‑140 (MQ=255)

                                                                                 gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  <  1:7994616/140‑1 (MQ=255)

                                                                                 gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  >  2:2519453/1‑140 (MQ=255)

                                                                                 gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  >  2:10585633/1‑140 (MQ=255)

                                                                                 gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  >  2:7031309/1‑140 (MQ=255)

                                                                                 gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  >  1:4264233/1‑140 (MQ=255)

                                                                                    tATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAAt                                               >  1:3973627/1‑140 (MQ=255)

                                                                                      ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATCAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc                                             >  2:9058510/1‑140 (MQ=255)

                                                                                      ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc                                             >  1:8071456/1‑140 (MQ=255)

                                                                                      ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc                                             >  1:3121395/1‑140 (MQ=255)

                                                                                      ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATGAATAAGCGTTCGGTGAGTGGCGTCGACAGCGGCGAATcc                                             >  1:8203803/1‑140 (MQ=255)

                                                                                      gtGACCGGCGACTGAACGGCAACAACAAACGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATCAGCGTTCGGTGACGGACGTCGACAGCGGCGAATcc                                             <  2:6564162/139‑1 (MQ=255)

                                                                                           cGGCGTCTGAACGGCAAAAACAAATGCTTGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACATTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                        >  1:5795830/1‑140 (MQ=255)

                                                                                           cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGc                                                                                        <  2:4393291/92‑1 (MQ=255)

                                                                                           cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                        >  2:10745355/1‑140 (MQ=255)

                                                                                           cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                        >  1:4779350/1‑140 (MQ=255)

                                                                                           cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                        >  2:8471340/1‑140 (MQ=255)

                                                                                           cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                        <  1:1684535/140‑1 (MQ=255)

                                                                                            ggagTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCGCCGAGGCCATTACGAAGCGTTCGGTGACTGAGGTCGACAGCGGCGAATCCGTATcc                                       >  2:10058112/4‑140 (MQ=255)

                                                                                              cGTCTGAACGGCAAAAACAAAAGCCTGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCAt                                     <  2:270960/140‑1 (MQ=255)

                                                                                              cGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCTCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCAt                                     <  2:6450035/140‑1 (MQ=255)

                                                                                              cGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAGTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCAt                                     >  1:2613948/1‑140 (MQ=255)

                                                                                                 cTGAACGTCAAAAACAAAAGCATTGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCTCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTcc                                  <  2:7899618/140‑1 (MQ=255)

                                                                                                   gAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCa                                <  1:1940703/140‑1 (MQ=255)

                                                                                                     aCGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACg                              <  2:5594891/140‑1 (MQ=255)

                                                                                                     aCGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACg                              <  1:6678125/140‑1 (MQ=255)

                                                                                                      cgccAAAAACAAAAGCATGGAGAAACGCCAGCGTTTTT‑T‑ACGCCGATATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACgg                             <  2:11180234/137‑1 (MQ=255)

                                                                                                       cgCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTAT‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAATAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGa                            <  2:2864025/139‑1 (MQ=255)

                                                                                                        gaaaaaaCAAACGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGAAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCCCGGaa                           <  1:9913154/138‑1 (MQ=255)

                                                                                                         cAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAg                          >  2:9439045/1‑140 (MQ=255)

                                                                                                         cAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAg                          >  1:3564438/1‑140 (MQ=255)

                                                                                                          aacaaCAAAAGCATGGAGAATCGCCAGCG‑TTTT‑TGACGCCGTTATAAAGCGCAAATTACACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGa                         <  2:9805926/137‑1 (MQ=255)

                                                                                                          aaaaaCAAAAGCATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGa                         <  1:7030433/140‑1 (MQ=255)

                                                                                                                     cATGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAAc              <  2:1718479/140‑1 (MQ=255)

                                                                                                                      aTGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAAcc             >  1:5402332/1‑140 (MQ=255)

                                                                                                                       tGGTGACTCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAAATAAACATTCGATCTCCGAGGCCATTCATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCg            <  2:10433395/140‑1 (MQ=255)

                                                                                                                       tGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCg            >  1:870074/1‑140 (MQ=255)

                                                                                                                       tGGTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCg            >  1:11279722/1‑140 (MQ=255)

                                                                                                                        ggTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGa           >  1:2794008/1‑140 (MQ=255)

                                                                                                                         gTGAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGAt          <  1:6561395/140‑1 (MQ=255)

                                                                                                                           gAATCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGt        <  2:130800/140‑1 (MQ=255)

                                                                                                                             aTCGCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGAGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGttg      >  2:10301788/1‑139 (MQ=255)

                                                                                                                                gCCAGCGTTTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTtcat   <  2:8227415/140‑1 (MQ=255)

ACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTT‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATC  >  NZ_CP025268/1873697‑1873964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`