Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 2,466,923 (C)8→9 intergenic (+242/‑123) fadL → / → CXP41_RS12955 long‑chain fatty acid transporter FadL/protein YfdF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,466,9151.C91.9% 655.2 / 53.6 213intergenic (+234/‑131)fadL/CXP41_RS12955long‑chain fatty acid transporter FadL/protein YfdF
Reads supporting (aligned to +/- strand):  ref base . (1/16);  new base C (25/169);  total (26/187)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 284 total aligned reads displayed.

TTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑T‑‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTA  >  NZ_CP025268/2466778‑2467051
                                                                                                                                              |                                                                                                                                             
ttGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑T‑‑c‑c                                                                                                                                           <  1:4686580/140‑1 (MQ=255)
 tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGTTA‑‑‑C‑T‑‑c‑cc                                                                                                                                          <  2:7389647/140‑1 (MQ=255)
 tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑T‑‑c‑cc                                                                                                                                          <  1:1985149/140‑1 (MQ=255)
 tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑T‑‑c‑cc                                                                                                                                          <  1:5554670/140‑1 (MQ=255)
 tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑T‑‑c‑cc                                                                                                                                          <  1:1677353/140‑1 (MQ=255)
 tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑T‑‑c‑cc                                                                                                                                          <  2:8445683/140‑1 (MQ=255)
  gCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑T‑‑c‑cc‑c                                                                                                                                        <  2:10815779/140‑1 (MQ=255)
   cAAACAGCCTGTTGGCAGCTTTCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGTGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑T‑‑c‑cc‑cc                                                                                                                                       <  2:7149310/140‑1 (MQ=255)
   cAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑T‑‑c‑cc‑cc                                                                                                                                       <  1:7895220/140‑1 (MQ=255)
   cAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑T‑‑c‑cc‑cc                                                                                                                                       <  1:5733298/140‑1 (MQ=255)
     atcAGCCTGTTGGGTGCTTGGCGTTTAAATTCATTTAGTGGTATGCTGAGATAATCCTGTATGGAAAGTCATTTTTTCAGTCGTTCTGCTCTTCCTTGTCAATTTGTTTACTTTTCTGCATTTCCAGGTTA‑‑‑C‑T‑‑c‑cc‑accc                                                                                                                                     <  2:9790181/138‑1 (MQ=255)
     accAGCCTGGTGGCAGCTTGCCCTTTCAAAACAATCAGCGGTATCCACAGACACACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑T‑‑c‑cc‑cccc                                                                                                                                     <  2:10253163/138‑1 (MQ=255)
     aaCAGCCTGTTGGCAGCTTGCCCTTTCCAATCAAGAAGCGGTATCCACAGACAACCCTGAAGGGAAAGGCATAATTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑T‑‑c‑cc‑cccc                                                                                                                                     <  1:8853973/140‑1 (MQ=255)
     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATCAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTATGCATTTCCAGGATA‑‑‑C‑T‑‑c‑cc‑cccc                                                                                                                                     <  1:7009298/140‑1 (MQ=255)
     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATCAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCAGATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑T‑‑c‑cc‑cccc                                                                                                                                     <  2:4325357/140‑1 (MQ=255)
     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑T‑‑c‑cc‑cccc                                                                                                                                     <  1:885321/140‑1 (MQ=255)
     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑T‑‑c‑cc‑cccc                                                                                                                                     <  2:1360755/140‑1 (MQ=255)
     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑T‑‑c‑cc‑cccc                                                                                                                                     <  2:10559113/140‑1 (MQ=255)
     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑T‑‑c‑cc‑cccc                                                                                                                                     <  2:10145349/140‑1 (MQ=255)
     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑T‑‑c‑cc‑cccc                                                                                                                                     <  1:11255797/140‑1 (MQ=255)
     aaCAGCCTGTTGGCAGCTTGCCCTTACAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑T‑‑c‑cc‑cccc                                                                                                                                     <  2:8045592/140‑1 (MQ=255)
     aaCAGCCTGTTGGCAGCGTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑T‑‑c‑cc‑cccc                                                                                                                                     <  2:2567301/140‑1 (MQ=255)
       cAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑T‑‑c‑cc‑cccccc                                                                                                                                   <  1:8312735/140‑2 (MQ=255)
       cAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑T‑‑c‑cc‑cccccc                                                                                                                                   <  1:9766299/140‑2 (MQ=255)
       cAGCCGGGTGGCAGCTGGCCCTTTCAACTCACTAAGCGGTATCCACAGACAAACCTGACGGGATTGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAGTTTTCTGCATTTCCAGGATA‑‑‑C‑T‑‑c‑cc‑cccccc                                                                                                                                   <  2:9029633/140‑2 (MQ=255)
        aGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑T‑‑c‑cc‑ccccccg                                                                                                                                  >  1:299784/1‑138 (MQ=255)
          ccTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCACAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑T‑‑c‑cc‑cccccctgg                                                                                                                                <  1:5432183/140‑5 (MQ=255)
          ccTGTTGGCAGCTTGCACTTTCAAATCAATCAGCGGTATCCACATCCACACCTGCAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGCGTTTACTTTTCTGCATTGCCAGGATC‑‑‑C‑T‑‑c‑cc‑cccccctgg                                                                                                                                <  2:6747274/140‑5 (MQ=255)
          ccTGTTGGCAGCGTGCCATTTCAAATCAATAAGCGGTATCCACAGTCAAACCTGAATGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑T‑‑c‑cc‑cccccctgg                                                                                                                                <  2:6049676/140‑5 (MQ=255)
                ggCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACACACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCGTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATtg                                                                                                                         <  2:5052452/140‑1 (MQ=255)
                ggCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATtg                                                                                                                         <  2:7839754/140‑1 (MQ=255)
                  cAGCTTGCCCTTTCAAATCACTAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑GGGCTATtgtg                                                                                                                       <  2:3750307/140‑1 (MQ=255)
                  cAGCTTGCCCTTTCAAATCAATACGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATtgtg                                                                                                                       <  1:5128849/140‑1 (MQ=255)
                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATtgtg                                                                                                                       <  1:10803426/140‑1 (MQ=255)
                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATtgtg                                                                                                                       <  2:3097591/140‑1 (MQ=255)
                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATtgtg                                                                                                                       <  2:886404/140‑1 (MQ=255)
                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATtgtg                                                                                                                       <  1:3919672/140‑1 (MQ=255)
                  cAGCTTGCCCATTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATtgtg                                                                                                                       <  2:212720/140‑1 (MQ=255)
                  cAGCTGGCCCTTTCAACGCACTCAGCGGAATCCACAGACAAAACTGAAGGGAAATGCATATGTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGTCTATtgtg                                                                                                                       <  2:9457723/140‑1 (MQ=255)
                    gcgtGCCCTTTCAAAACAATAAGCGGGCTCCACAGCCAAACCTGAAGGGCACGGCATATTTTCAGGCGTGCTGCGCGTCCTTCTCAAAGAGTATACTTTTCTGCATGTCCAGGATACGCC‑C‑‑C‑CC‑CCCCC‑‑TGGCTATtgtg                                                                                                                       <  2:5543795/137‑1 (MQ=255)
                    gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAATGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTgcg                                                                                                                     <  2:4730385/140‑1 (MQ=255)
                    gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTgcg                                                                                                                     <  1:4329171/140‑1 (MQ=255)
                    gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTgcg                                                                                                                     <  1:10566668/140‑1 (MQ=255)
                     cTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TCCC‑CC‑CCCCC‑‑TGGCTATTGTgcg                                                                                                                     <  1:6243091/140‑1 (MQ=255)
                        gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCa                                                                                                                 >  2:8170433/1‑140 (MQ=255)
                        gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCa                                                                                                                 >  2:9945499/1‑140 (MQ=255)
                         cccTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TCCC‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCa                                                                                                                 <  1:8377774/140‑1 (MQ=255)
                             ttCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATacac                                                                                                            <  1:1345750/140‑1 (MQ=255)
                              tCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACt                                                                                                           <  2:1699576/140‑1 (MQ=255)
                              acAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACt                                                                                                           <  2:6493889/139‑1 (MQ=255)
                               cAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTc                                                                                                          >  1:5674488/1‑140 (MQ=255)
                               cAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTc                                                                                                          <  1:444296/140‑1 (MQ=255)
                                  aTCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCaaa                                                                                                       <  1:7316308/140‑1 (MQ=255)
                                     aaTAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGATTTTGTGCGCTCATACACTCAAATTa                                                                                                    <  2:758449/140‑1 (MQ=255)
                                         cgCGGTATCCACAGACAAACCTGATGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGa                                                                                                <  1:10397076/139‑1 (MQ=255)
                                         aGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGa                                                                                                <  1:5485059/140‑1 (MQ=255)
                                         aGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑T‑‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                               <  2:3181330/140‑1 (MQ=255)
                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                               <  2:8538012/140‑1 (MQ=255)
                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCAGTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                               <  2:526682/140‑1 (MQ=255)
                                          gCGGTATCAACAGACTACCCTGAAGGGTATGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTATGCATTTCCAGGATA‑‑‑C‑T‑‑CACC‑CCCCC‑‑TGGCTATTGTGAGCTCATACACTCAAATTAAAGAt                                                                                               <  2:110794/140‑1 (MQ=255)
                                             gTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCTTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAgg                                                                                            <  2:81205/140‑1 (MQ=255)
                                               aTCCACAGACAAACCTGCAGGGAAACGCATAATTACAGGCGATCTGCTCGTCCTTCTCAAAGATTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGGGCGCGCATACACTCAAATTAAAGATAGCtt                                                                                          <  2:2742733/140‑1 (MQ=255)
                                               aTCCACAGACAAACCTGAAGTGAAAGGAATTTTTTCATGCGTTCTGCTCGTCCTTCTAAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                          <  2:6353407/140‑1 (MQ=255)
                                                    caGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaa                                                                                     <  1:10883939/140‑1 (MQ=255)
                                                     aGACAAACCTGAAGTGAAATTCATATTTTCATTCGTTCTGCTCGTCATTCTAAAAGAGTTTACTTTTCTGCATTTCCATGATA‑‑‑C‑T‑‑C‑CC‑CCCCCCATGGCTATTGTGCGCTCATTCACTCAAATTAAAGATAGGTTCtaaa                                                                                     <  2:9327866/140‑1 (MQ=255)
                                                          aaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATg                                                                               >  2:1859387/1‑140 (MQ=255)
                                                          aaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATg                                                                               >  2:6876689/1‑140 (MQ=255)
                                                            ccTTAAGGTAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCAGTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATACATGAg                                                                             <  2:8683909/140‑1 (MQ=255)
                                                            ccTGACGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                             <  1:10208626/140‑1 (MQ=255)
                                                            ccTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                             <  1:6780832/140‑1 (MQ=255)
                                                            ccTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                             <  1:703032/140‑1 (MQ=255)
                                                            ccTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                             <  1:3783575/140‑1 (MQ=255)
                                                            ccTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑GGGCTATTGTGCGCTCATACCCTCAAATTACAGATAGTTTCTAAATAAATGAg                                                                             <  2:5692323/140‑1 (MQ=255)
                                                                   ggAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑CAC‑‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATAAACTCAAATTAAAGTTAGGTTCTAAATAAATGAGCGttttt                                                                      >  2:3031661/1‑140 (MQ=255)
                                                                     aaaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTg                                                                    >  2:9555449/1‑140 (MQ=255)
                                                                      aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                   <  2:3300768/140‑1 (MQ=255)
                                                                      aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                   <  2:2845996/140‑1 (MQ=255)
                                                                            tatTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCt                                                             <  1:2246384/140‑1 (MQ=255)
                                                                             atTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                                            <  1:8505264/140‑1 (MQ=255)
                                                                             atTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCGCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                                            <  1:3565953/140‑1 (MQ=255)
                                                                                  ccggCGTTCTGCTCGACCTTCGCAAAGAGTTTACTTTTCTGCATGGCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCGATTGTGCGCTCATACACTCACCTTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                       <  2:3980299/138‑1 (MQ=255)
                                                                                  cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                       <  1:7105067/140‑1 (MQ=255)
                                                                                  cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                       <  1:7473418/140‑1 (MQ=255)
                                                                                    ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                     <  1:1576802/140‑1 (MQ=255)
                                                                                    ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                     <  1:9398558/140‑1 (MQ=255)
                                                                                    ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                     <  1:678953/140‑1 (MQ=255)
                                                                                       gTTCTGCTCGTCCTTCTCTTTGAGTTGACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGTTAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                                  <  2:1161343/140‑1 (MQ=255)
                                                                                       gTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTTCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATATATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                                  <  1:9050936/140‑1 (MQ=255)
                                                                                       gTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                                  <  1:6453664/140‑1 (MQ=255)
                                                                                         tCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTa                                                <  1:1797212/140‑1 (MQ=255)
                                                                                         tCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTa                                                <  2:1231985/140‑1 (MQ=255)
                                                                                             cTCGCCCTTCTCACAGAGTTTACTTTTCTGCAATTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAAATAAAGATAGGTTCGAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatat                                            <  2:7012692/140‑1 (MQ=255)
                                                                                                          aaGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCGCATACACTCCAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTTTTAGTCTATTTCATTAGGTAATATATATTTGTAACaa                               >  1:5336588/1‑140 (MQ=255)
                                                                                                               tttACTTTTCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAaatcaa                          >  2:4899351/1‑140 (MQ=255)
                                                                                                                     tttCTGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCaaaa                    <  1:657693/140‑1 (MQ=255)
                                                                                                                         tGCATTTCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCACTCAAAATGGa                >  1:10099760/1‑140 (MQ=255)
                                                                                                                          gCATTTACATGATA‑‑‑C‑T‑‑C‑CCACCAAC‑‑TGGCTATTGTTCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGATCTTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGaa               <  2:10334473/140‑1 (MQ=255)
                                                                                                                               tCCAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATCCACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATaaaa          <  1:11143995/140‑1 (MQ=255)
                                                                                                                                 cAGGATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑TTGCTATTGTGCGCTCATACACTAAACTTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGACTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATc        <  1:7153574/140‑1 (MQ=255)
                                                                                                                                    gATA‑‑‑C‑TC‑C‑CC‑CCCCC‑‑GGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATg     <  1:8931540/140‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                             
TTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATA‑‑‑C‑T‑‑C‑CC‑CCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTA  >  NZ_CP025268/2466778‑2467051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑