BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,769,852	+C	intergenic (‑52/‑89)	yfjM ← / → rnlA	DUF1524 domain‑containing protein/type II toxin‑antitoxin system toxin mRNA endoribonuclease RnlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,769,848	1	.	C	96.9%	1716.9 / 42.4	518	intergenic (‑48/‑93)	yfjM/rnlA	DUF1524 domain‑containing protein/type II toxin‑antitoxin system toxin mRNA endoribonuclease RnlA
Reads supporting (aligned to +/- strand): ref base . (4/12); new base C (227/274); total (231/287)
Fisher's exact test for biased strand distribution p-value = 1.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 551 total aligned reads displayed.

TCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑C‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCA  >  NZ_CP025268/2769710‑2769982

 cTTGTCTGCTGGCGACTAATGACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑c‑c                                                                                                                                       <  2:462246/140‑1 (MQ=255)

 cTTGTCTGCTGGCGAATAATTACTAAGTCACGTGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑c‑c                                                                                                                                       <  2:9408880/140‑1 (MQ=255)

 cTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑c‑c                                                                                                                                       <  1:6976820/140‑1 (MQ=255)

 cTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑c‑c                                                                                                                                       <  1:3802483/140‑1 (MQ=255)

 cTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑c‑c                                                                                                                                       <  2:10786766/140‑1 (MQ=255)

 cTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑c‑c                                                                                                                                       <  1:3228113/140‑1 (MQ=255)

   tGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGGGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑c‑ccc                                                                                                                                     <  2:208092/140‑1 (MQ=255)

           ggCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAg                                                                                                                             <  1:5370815/140‑1 (MQ=255)

                         aaGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAg                                                                                                               <  1:5721782/140‑1 (MQ=255)

                         aaGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAg                                                                                                               <  1:1264506/140‑1 (MQ=255)

                              tCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGACACGATGGACAGCATCCCCCATTGTGGGGGCTGCGCCGCCGGTAGTCGATCGGGCCCTGTCGTTGGGGGG‑CGCCC‑GAGTCAGGTGGCGAAAGTTAGCagtg                                                                                                          >  2:9805774/1‑137 (MQ=255)

                              tCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAAt                                                                                                          <  1:2771000/140‑1 (MQ=255)

                              tCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAAt                                                                                                          <  1:6573022/140‑1 (MQ=255)

                               cGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGTCGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑GATTCAGGTGGCTAAATTTAGCAAATc                                                                                                         >  1:1555971/1‑140 (MQ=255)

                               cGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGGAGACGATCCTGCCCTTGAGTGGGAGTGCC‑CCC‑TTTTCGGGTGGCTAAATTTAGCAAATc                                                                                                         >  1:934810/1‑140 (MQ=255)

                                 agctgctgcTTGGGGAGATCAGGAAGAGGTTACCGAAACTATGGACATCATCCCCCATGTGGTGGTCTGGCCCCCCGTTAGACGATCCTGCCCGATAGTTGGAGTGCC‑CCC‑TCTTCAGGTGGATAAATTTCGCAAATCaa                                                                                                       <  2:8730913/137‑1 (MQ=255)

                                   ttGCTGCTTGGTGAGATCATGAAGAGGTTACCGAAACTATGGACATAATCCCACATGTTGGGGCATTCCCCCACGGTAGACTATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                     <  2:3163133/140‑1 (MQ=255)

                                   ttGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                     <  2:3742815/140‑1 (MQ=255)

                                   ttGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                     <  2:5676594/140‑1 (MQ=255)

                                   ttGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                     <  2:7661795/140‑1 (MQ=255)

                                   ttGCTGCTTGGTGAGATCAGGAAGAGGTGACCGAAACGATGGACATCATCCCCCATTTTGGGGACTGCCCCCCCGGTAGCCGATCCTGCCCGATCGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCACTa                                                                                                     <  2:10441909/140‑1 (MQ=255)

                                   gtgctgcTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                     <  2:2383196/139‑1 (MQ=255)

                                     gctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATaca                                                                                                   <  1:7722026/140‑1 (MQ=255)

                                      ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCGTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGGTAAATTTAGCAAATCAATagac                                                                                                  >  1:7290773/1‑140 (MQ=255)

                                      ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATacac                                                                                                  >  2:10631900/1‑140 (MQ=255)

                                      ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATacac                                                                                                  >  1:4293737/1‑140 (MQ=255)

                                      ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGGTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATacac                                                                                                  >  2:5923345/1‑140 (MQ=255)

                                           tGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAg                                                                                             <  2:1441245/140‑1 (MQ=255)

                                             gTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCTGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                                                                           <  2:3556464/140‑1 (MQ=255)

                                             gTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                                                                           <  2:1257007/140‑1 (MQ=255)

                                             gTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                                                                           <  1:8787352/140‑1 (MQ=255)

                                                agaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                                                                        >  2:8438081/1‑140 (MQ=255)

                                                agaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                                                                        <  1:3405232/140‑1 (MQ=255)

                                                agaTCAGGAAGAGGTTACAGAAACGATGTACATCATCCCCCATTTTGTGGTCTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                                                                        <  2:2258572/140‑1 (MQ=255)

                                                   tCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTATATTTAGCAAATCAATCCACTTCAGGGGGGtat                                                                                     >  1:10755596/1‑140 (MQ=255)

                                                   tCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                     <  2:7315659/124‑1 (MQ=255)

                                                   tCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGtat                                                                                     <  1:4707077/140‑1 (MQ=255)

                                                        aaGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTg                                                                                <  2:8289619/140‑1 (MQ=255)

                                                        aaGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTg                                                                                <  2:5195029/140‑1 (MQ=255)

                                                         agagGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGt                                                                               <  1:3644659/140‑1 (MQ=255)

                                                            ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTaga                                                                            >  2:5318129/1‑140 (MQ=255)

                                                            ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTaga                                                                            <  1:8443635/140‑1 (MQ=255)

                                                            ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATACAGGTGGCTAAATTTAGCAAATCAAAACACTTCAGGGGGGTATTATTGTaga                                                                            >  2:11055211/1‑140 (MQ=255)

                                                               tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGtt                                                                         >  2:8598421/1‑140 (MQ=255)

                                                               tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGtt                                                                         <  1:10108072/140‑1 (MQ=255)

                                                                 ccGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTc                                                                       >  1:6876088/1‑140 (MQ=255)

                                                                     caCGATGGACATCATCCCCCATTGTGTGGACTGCCCCCCCGCTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCa                                                                   <  2:400893/139‑1 (MQ=255)

                                                                     aaCGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCa                                                                   <  1:7895969/140‑1 (MQ=255)

                                                                      aCGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCat                                                                  <  1:1847864/140‑1 (MQ=255)

                                                                          tGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑C‑CCCTTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGt                                                              >  2:6712944/1‑140 (MQ=255)

                                                                          tGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTCGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGt                                                              <  1:9535555/140‑1 (MQ=255)

                                                                          aggACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTAGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGt                                                              <  1:2953608/139‑1 (MQ=255)

                                                                            gACATCATCCCCCCTTTTGTCGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGttt                                                            <  2:3849245/140‑1 (MQ=255)

                                                                                 catcCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTCTAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATg                                                       >  1:4411850/1‑140 (MQ=255)

                                                                                 catcCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAACTTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATg                                                       <  2:2816824/140‑1 (MQ=255)

                                                                                 catcCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                      <  2:4869197/109‑1 (MQ=255)

                                                                                 catcCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATg                                                       <  2:2692740/140‑1 (MQ=255)

                                                                                 catcCCCCATTGTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGGTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCATATCAATACACTTCAGGGGGGGATTATTGGAGAGTTTCCGCATATGGTTCTATg                                                       >  2:3374998/1‑140 (MQ=255)

                                                                                   tcCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATAGGTTTCTATggg                                                     >  1:5164526/1‑140 (MQ=255)

                                                                                    cccccATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCGTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGa                                                    >  2:296801/1‑140 (MQ=255)

                                                                                    cccccATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGa                                                    >  2:6931222/1‑140 (MQ=255)

                                                                                       ccATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATcc                                                 >  2:9599339/1‑140 (MQ=255)

                                                                                          ttttGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAgg                                              <  2:7513615/140‑1 (MQ=255)

                                                                                                ggACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGa                                        <  1:497454/140‑1 (MQ=255)

                                                                                                    tGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATc                                                  <  2:4959747/126‑1 (MQ=255)

                                                                                                    tGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAAt                                    >  2:9074000/1‑140 (MQ=255)

                                                                                                      cccccccGGTAGACGATCCTGCCCTCTAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAAGTTAGCAAATCACTCCACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACACTCa                                  >  2:7130546/1‑140 (MQ=255)

                                                                                                      cccccccGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCa                                  >  2:10029344/1‑140 (MQ=255)

                                                                                                      cccccccGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCa                                  >  2:3968603/1‑140 (MQ=255)

                                                                                                         ccccGGTAGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGa                               <  1:9138988/140‑1 (MQ=255)

                                                                                                                aGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCTGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGATATGACAATCAGGAGTTACat                        >  1:8943943/1‑139 (MQ=255)

                                                                                                                aGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaa                        <  2:1088017/140‑1 (MQ=255)

                                                                                                                aGACGATCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaa                        <  1:1006456/140‑1 (MQ=255)

                                                                                                                   cGATCCAGCCCTACAGTGGGAGTGCC‑CCC‑TCTTCCGGTGGCTAACTATAGCCAATCATTACACAACCGGTGGGTATTATTGTATAGTTTCCCGAGATGTTTCTATGGGATCAAGTAAATGACAATCAGGAGTTACaaaaa                     <  2:11305349/140‑1 (MQ=255)

                                                                                                                     aTCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACt                   >  1:6249563/1‑140 (MQ=255)

                                                                                                                     aTCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACt                   >  1:6136746/1‑140 (MQ=255)

                                                                                                                     aTCCTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACt                   >  1:45326/1‑140 (MQ=255)

                                                                                                                       ccTGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTa                 >  1:11320372/1‑140 (MQ=255)

                                                                                                                         tGCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTaaa               >  1:7510703/1‑140 (MQ=255)

                                                                                                                          gCCCTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAAt              <  1:2226458/140‑1 (MQ=255)

                                                                                                                          gCCCAATAGTAGGAGTGCC‑CCC‑TAGTCAGGTGGCTAAATAGAGCACATCAATACACTTCAGGGGGGTATGCTTGTAGAGTTTCCCCATATGTTTCTAAGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAAt              <  2:6393801/140‑1 (MQ=255)

                                                                                                                           cccTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATc             <  1:398251/140‑1 (MQ=255)

                                                                                                                           cccTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATc             >  2:6124156/1‑140 (MQ=255)

                                                                                                                             cTATAGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTg           >  1:5097138/1‑140 (MQ=255)

                                                                                                                              tataGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTgg          >  2:4541011/1‑140 (MQ=255)

                                                                                                                               ataGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGt         <  1:2054886/140‑1 (MQ=255)

                                                                                                                                taGTTGGAGTGCC‑CCC‑TATTCCGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc        >  2:7874349/1‑140 (MQ=255)

                                                                                                                                taGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAACTGACAATCAGGAGTTACAAAAACTTAAATCTGGTc        >  2:9271455/1‑140 (MQ=255)

                                                                                                                                taGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc        >  2:4358805/1‑140 (MQ=255)

                                                                                                                                taGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc        >  1:2600191/1‑140 (MQ=255)

                                                                                                                                taGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc        >  2:1917464/1‑140 (MQ=255)

                                                                                                                                taGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTACCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc        >  2:478429/1‑140 (MQ=255)

                                                                                                                                taGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAAGACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc        <  1:9759246/140‑1 (MQ=255)

                                                                                                                                taGTTGGAGTGCC‑CCC‑TATTCAGGTGGCAAAATTAAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAAACAGGAGTTACAAAAACTTAAATCTGGTc        >  2:6558558/1‑140 (MQ=255)

                                                                                                                                gaGTTGGAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc        <  1:8107277/139‑1 (MQ=255)

                                                                                                                                     ggAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACACTCAGGAGTTACAAAAACTTAAATCTGGTCAGGGc   >  1:8667536/1‑140 (MQ=255)

                                                                                                                                     ggAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGc   >  1:11076788/1‑140 (MQ=255)

                                                                                                                                     ggAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATNATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGc   >  1:5987310/1‑140 (MQ=255)

                                                                                                                                      gAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCa  <  1:8599391/140‑1 (MQ=255)

                                                                                                                                      gAGTGCC‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCa  <  1:10235227/140‑1 (MQ=255)

TCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑C‑CCC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCA  >  NZ_CP025268/2769710‑2769982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`