BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,858,752	+T	pseudogene (395/2078 nt)	flhA →	formate hydrogenlyase transcriptional activator FlhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,858,748	1	.	T	97.2%	2222.6 / 44.1	607	pseudogene (391/2078 nt)	flhA	formate hydrogenlyase transcriptional activator FlhA
Reads supporting (aligned to +/- strand): ref base . (9/8); new base T (296/293); total (306/301)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 698 total aligned reads displayed.

ACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TA‑T‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGTCG  >  NZ_CP025268/2858609‑2858882

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGACGGGC‑A‑TA‑t‑ttt                                                                                                                                     >  1:3552794/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TA‑t‑ttt                                                                                                                                     >  1:172699/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TA‑t‑ttt                                                                                                                                     >  1:421473/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TA‑t‑ttt                                                                                                                                     >  1:6777447/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TA‑t‑ttt                                                                                                                                     >  1:6990770/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TA‑t‑ttt                                                                                                                                     >  1:8423068/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TA‑t‑ttt                                                                                                                                     >  1:9152973/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TA‑t‑ttt                                                                                                                                     >  2:2922860/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TA‑t‑ttt                                                                                                                                     >  2:6840660/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TA‑t‑ttt                                                                                                                                     >  2:7304514/1‑140 (MQ=255)

       aTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TA‑t‑ttttgg                                                                                                                                  <  1:10145899/140‑4 (MQ=255)

        ttttGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAACATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TA‑t‑ttttggt                                                                                                                                 >  1:4865506/1‑136 (MQ=255)

        ttttGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TA‑t‑ttttggt                                                                                                                                 >  2:460009/1‑136 (MQ=255)

           tGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGc                                                                                                                              >  2:8826006/1‑140 (MQ=255)

            gTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAACTTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCt                                                                                                                             <  2:4347216/140‑1 (MQ=255)

              cgcgCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCtgt                                                                                                                           <  2:9705100/140‑1 (MQ=255)

              cgcgCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCtgt                                                                                                                           <  2:9171912/140‑1 (MQ=255)

                cgcCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGa                                                                                                                         >  2:5767686/1‑140 (MQ=255)

                  cccTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAAt                                                                                                                       >  2:788037/1‑140 (MQ=255)

                      gATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTAt                                                                                                                   <  1:295514/140‑1 (MQ=255)

                      gATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTAt                                                                                                                   <  1:5356417/140‑1 (MQ=255)

                       aTACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAAGTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTAtt                                                                                                                  >  2:1389352/1‑140 (MQ=255)

                             tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTAt                                                                                                            >  1:11043677/1‑140 (MQ=255)

                             tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTAt                                                                                                            <  1:8524613/140‑1 (MQ=255)

                             tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTAt                                                                                                            >  2:5997145/1‑140 (MQ=255)

                             tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTAt                                                                                                            >  1:1950039/1‑140 (MQ=255)

                               cATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGa                                                                                                          >  1:7728759/1‑140 (MQ=255)

                               cATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGa                                                                                                          >  2:1160299/1‑140 (MQ=255)

                               cATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGa                                                                                                          >  1:5797075/1‑140 (MQ=255)

                               cATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGa                                                                                                          >  1:1219944/1‑140 (MQ=255)

                               cATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGa                                                                                                          >  2:473144/1‑140 (MQ=255)

                                 ttGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACg                                                                                                        >  2:2207957/1‑140 (MQ=255)

                                   gCATTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GTTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  2:4404157/140‑1 (MQ=255)

                                   gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGTCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  2:6519278/140‑1 (MQ=255)

                                   gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  2:5131450/140‑1 (MQ=255)

                                   gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  1:10022229/140‑1 (MQ=255)

                                   gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  1:5160177/140‑1 (MQ=255)

                                   gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  1:2400363/140‑1 (MQ=255)

                                   gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTAGTTATGACGAt                                                                                                      <  2:549740/140‑1 (MQ=255)

                                   gCAGTTACGAAGAATTGTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  2:3636710/140‑1 (MQ=255)

                                    cAGTTACGNAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCANAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATc                                                                                                     >  1:5996660/1‑140 (MQ=255)

                                     aGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCg                                                                                                    >  1:7972312/1‑140 (MQ=255)

                                       ttACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGcc                                                                                                  >  1:1048660/1‑140 (MQ=255)

                                       ttACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGcc                                                                                                  >  2:865972/1‑140 (MQ=255)

                                       ttACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑GATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGcc                                                                                                  >  1:563814/1‑140 (MQ=255)

                                         aCGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGcccc                                                                                                <  1:7273738/140‑1 (MQ=255)

                                         aCGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGcccc                                                                                                >  2:4006606/1‑140 (MQ=255)

                                           gaagaaTTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTg                                                                                              <  2:3291774/140‑1 (MQ=255)

                                           gaagaaTTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTg                                                                                              <  2:10705309/140‑1 (MQ=255)

                                              gaaTTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAg                                                                                           >  2:2861291/1‑140 (MQ=255)

                                              gaaTTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAg                                                                                           <  1:6321359/140‑1 (MQ=255)

                                                      tgAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAag                                                                                   >  1:2327312/1‑140 (MQ=255)

                                                              ggCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAAt                                                                           <  1:8945880/140‑1 (MQ=255)

                                                              ggCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAAt                                                                           <  2:7791833/140‑1 (MQ=255)

                                                              ggCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAAt                                                                           <  2:3489155/140‑1 (MQ=255)

                                                                ccGCAGCTGGACGNAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCg                                                                         >  1:9716259/1‑140 (MQ=255)

                                                                ccGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCg                                                                         >  2:1591279/1‑140 (MQ=255)

                                                                ccGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCg                                                                         >  1:3026355/1‑140 (MQ=255)

                                                                      cTGGACGCAGGTGGGCTATACCCAAACTTTGGTCACGATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TA‑TATTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCa                                                                   <  2:7989919/140‑1 (MQ=255)

                                                                        ggACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCaaa                                                                 <  1:2807505/140‑1 (MQ=255)

                                                                              aGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTa                                                           >  1:9316022/1‑140 (MQ=255)

                                                                              aGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTa                                                           >  1:436766/1‑140 (MQ=255)

                                                                               ggTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTAc                                                          <  1:9577856/140‑1 (MQ=255)

                                                                                   ggCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAg                                                      >  2:3032678/1‑140 (MQ=255)

                                                                                        taCCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGACAAAGAGTTCAATCGTCTGAACACATTTACGCAGATCgg                                                 >  2:6218761/1‑139 (MQ=255)

                                                                                        aaCCCAAAATAAGCTCACGATTCCCTGATGCCACTCGCCGCCGAAGTCC‑A‑AATT‑TTT‑GGAGGCGCTGAATTTAATCGTTAAGACGAACGCCCCAGGAGCGAAAAAGAGTTCAATCGTCAGCAAACAATAACGCAGATCGt                                                 >  2:10974528/2‑140 (MQ=255)

                                                                                          cccAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGttt                                               <  1:1864802/140‑1 (MQ=255)

                                                                                          cccAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGttt                                               <  2:9444636/140‑1 (MQ=255)

                                                                                             aaaaTTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTg                                            >  2:1017414/1‑140 (MQ=255)

                                                                                              aaaTTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTgt                                           <  1:141030/140‑1 (MQ=255)

                                                                                              aaaTTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTgt                                           <  2:9230078/140‑1 (MQ=255)

                                                                                                 tttGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTgtcgt                                        <  1:8773993/140‑1 (MQ=255)

                                                                                                  ttGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTgtcgtc                                       >  1:9888127/1‑140 (MQ=255)

                                                                                                    ggTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAc                                     >  1:9955274/1‑140 (MQ=255)

                                                                                                      tCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCg                                   <  1:3875145/140‑1 (MQ=255)

                                                                                                         cgaTTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGATTCTGTCGTCACCGAAc                                <  1:4636932/138‑1 (MQ=255)

                                                                                                         cTATTGCCTGATGCCACTGGCGGCGGAAGGGCAA‑AA‑T‑TTT‑GGGGGCTGTCAATTTATTCGTTCTGCCGCACGCCCCTGCAGCGAAAAAGAGTTCTATCGTCGGCAACCCAATACCCAGATCGATTCTGTCGTCACCGAAc                                <  2:11231111/140‑1 (MQ=255)

                                                                                                         cTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                                <  2:5274428/140‑1 (MQ=255)

                                                                                                         cTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                                >  2:636667/1‑140 (MQ=255)

                                                                                                         cTATTGCCTGAAGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                                <  1:6572978/140‑1 (MQ=255)

                                                                                                              gCCTGATGCATCTGTCGGCGGAAGTGC‑ATTT‑T‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATc                           <  2:7605736/140‑1 (MQ=255)

                                                                                                                    tGCCACTTGCGGCGGAAGGGC‑A‑TA‑T‑TTTAGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGGTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACAGAACAAATCCAGAGc                     >  2:7033613/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGGC‑C‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  2:8986588/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCATCGAACAAATCCAGAGc                     >  2:4932097/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  1:5568432/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  2:3841939/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  1:7488745/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGGTATGACGATCGGCCCTGGAGCGAACAAGAGGTCTCTCGTCTGCAGTGATTGTCGCGGATCGTGTCTGTCGTCAGCGAGCAATTCCagggg                     >  2:8386196/1‑137 (MQ=255)

                                                                                                                     gCCACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGcc                    <  2:6081387/140‑1 (MQ=255)

                                                                                                                     gCCACTGGCGGCGGAAGGGC‑A‑TA‑TATTT‑GGTGGCAGATAATTTATTCGTTATCAAGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGACTGCAAACATTTACGCAGATCGTTTCGGTCGTCACCGAACAAATCCAGAGcc                    <  2:10446623/140‑1 (MQ=255)

                                                                                                                      ccACTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCcg                   >  1:11221116/1‑140 (MQ=255)

                                                                                                                         cTGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGcgt                <  1:2182362/140‑1 (MQ=255)

                                                                                                                          tGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGcgtc               <  1:1075437/140‑1 (MQ=255)

                                                                                                                          tGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGcgtc               <  2:1692449/140‑1 (MQ=255)

                                                                                                                          tGGCGGCGGAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTAGTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGcgtc               <  2:8192116/140‑1 (MQ=255)

                                                                                                                              ggcggAAGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTa           >  2:190684/1‑140 (MQ=255)

                                                                                                                                    aGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATg     <  2:857464/140‑1 (MQ=255)

                                                                                                                                    aGGGC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATg     <  2:2371118/140‑1 (MQ=255)

                                                                                                                                     gggC‑A‑TATT‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGt    <  1:6488552/140‑1 (MQ=255)

                                                                                                                                     gggC‑A‑TATT‑TTT‑GGTGGCTGTGAATTGAGTCGTTATGACGAGCGCCCCTGGAGCGAAAAAGAGTTCAAGCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGt    <  1:2621062/140‑1 (MQ=255)

ACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑A‑TA‑T‑TTT‑GGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGTCG  >  NZ_CP025268/2858609‑2858882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`