Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 320,615 (G)6→7 intergenic (+482/‑44) rclR → / → ykgE reactive chlorine‑specific transcriptional regulator RclR/heterodisulfide reductase‑related iron‑sulfur binding cluster

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268320,6091.G94.4% 1532.6 / 81.0 467intergenic (+476/‑50)rclR/ykgEreactive chlorine‑specific transcriptional regulator RclR/heterodisulfide reductase‑related iron‑sulfur binding cluster
Reads supporting (aligned to +/- strand):  ref base . (15/11);  new base G (180/261);  total (195/272)
Fisher's exact test for biased strand distribution p-value = 1.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 490 total aligned reads displayed.

AGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑T‑G‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAAC  >  NZ_CP025268/320470‑320743
                                                                                                                                               |                                                                                                                                         
aGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑t                                                                                                                                            <  1:9578542/140‑1 (MQ=255)
aGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑t                                                                                                                                            >  2:6001581/1‑140 (MQ=255)
  cGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGTGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCAAATTTATTGATGATTGATCCC‑CC‑GG‑T‑g‑g                                                                                                                                        <  1:8562506/140‑1 (MQ=255)
  cGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑T‑g‑g                                                                                                                                        <  2:11091624/140‑1 (MQ=255)
    aTGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑T‑g‑gg‑g                                                                                                                                     >  2:1066042/1‑140 (MQ=255)
      gCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑T‑g‑gg‑gg‑g                                                                                                                                  >  1:6936782/1‑140 (MQ=255)
            agaaaTAAAATTAGTTAGTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACa                                                                                                                             <  2:3060275/140‑1 (MQ=255)
             gaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACAt                                                                                                                            <  1:6709033/140‑1 (MQ=255)
              acaTAAATTTAGTGAGTTCTTTTGACGTTTGGATGAAAGCTTCTGATTTGGCTTACAAATGAGGGGTGGTTTGTTGCTTGCCAATTAAAATAGAACGCCATATTTATTGATGATTGATCCC‑CA‑GGAG‑G‑GG‑GG‑GAAACATc                                                                                                                           <  2:5441549/138‑1 (MQ=255)
              aaaTAAAATTATTTATTGCAATTTACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGATATCCAATTAAAAAAGAACGCCATATTTATTGATTATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATc                                                                                                                           <  2:4505060/140‑1 (MQ=255)
              aaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATc                                                                                                                           <  2:927926/140‑1 (MQ=255)
                  aaaaTTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑T‑G‑GGTGG‑GAATCATCGGGc                                                                                                                       <  2:4908473/140‑1 (MQ=255)
                        aGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAAt                                                                                                                 >  1:8759144/1‑140 (MQ=255)
                            aTTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGc                                                                                                             <  1:8263173/140‑1 (MQ=255)
                             ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCa                                                                                                            <  2:2277197/140‑1 (MQ=255)
                             ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCa                                                                                                            <  1:5863443/140‑1 (MQ=255)
                              tGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCAt                                                                                                           >  2:6202844/1‑140 (MQ=255)
                               gCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATg                                                                                                          <  2:483708/140‑1 (MQ=255)
                                   ttGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAAATAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGt                                                                                                      >  1:8447951/1‑140 (MQ=255)
                                    tGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTa                                                                                                     >  2:9015629/1‑140 (MQ=255)
                                     gACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTaa                                                                                                    <  2:1834836/140‑1 (MQ=255)
                                     gACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTaa                                                                                                    <  1:9734316/140‑1 (MQ=255)
                                     gACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTaa                                                                                                    <  1:1340360/140‑1 (MQ=255)
                                     gACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTaa                                                                                                    <  2:428503/140‑1 (MQ=255)
                                      aCGTTTGGATGAAAGAGTATCATGTGTCATACACATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATAGGGATGGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GACACCTCGGGCATACAGGGGCATGAAGtcct                                                                                                   <  2:1599099/140‑4 (MQ=255)
                                      aCGTCTGGATGACAGATTATCCATTGTCCTACCAATGAGGGGAGGTAGGGAGCTCGCCAATTAACCAACAACGCCAGCTTTCTTGATGTTTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCAGGAAGTAAt                                                                                                   <  2:9939962/140‑1 (MQ=255)
                                        gTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATgg                                                                                                 <  1:11130432/140‑1 (MQ=255)
                                        gTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATgg                                                                                                 <  1:6328107/140‑1 (MQ=255)
                                           tGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGt                                                                                              <  2:3469846/140‑1 (MQ=255)
                                            ggATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTa                                                                                             <  2:9741698/140‑1 (MQ=255)
                                              aTGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTAtt                                                                                           <  1:7332214/140‑1 (MQ=255)
                                                gAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAg                                                                                         <  1:2718346/140‑1 (MQ=255)
                                                      ttATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGa                                                                                   <  2:4172890/140‑1 (MQ=255)
                                                      atATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATTTTTATTGATGATTGCTCCC‑CC‑TG‑T‑GTGG‑GT‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGa                                                                                   <  2:6788315/139‑1 (MQ=255)
                                                          cATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGt                                                                               <  2:79891/140‑1 (MQ=255)
                                                           aTTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTc                                                                              <  1:1496675/140‑1 (MQ=255)
                                                               gTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAtt                                                                          <  1:1726416/140‑1 (MQ=255)
                                                               gTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAtt                                                                          >  2:2082627/1‑140 (MQ=255)
                                                               gTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAtt                                                                          >  2:3615520/1‑140 (MQ=255)
                                                                  aTACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCt                                                                       <  1:9041535/140‑1 (MQ=255)
                                                                    aCAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCttt                                                                     >  1:10036724/1‑140 (MQ=255)
                                                                    aCAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCttt                                                                     <  2:10808302/140‑1 (MQ=255)
                                                                    aCAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCttt                                                                     >  2:7535707/1‑140 (MQ=255)
                                                                    aCAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCttt                                                                     <  1:245167/140‑1 (MQ=255)
                                                                     cAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTg                                                                    <  1:863849/140‑1 (MQ=255)
                                                                      aaaTGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGt                                                                   <  2:9575871/140‑1 (MQ=255)
                                                                      aaaTGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGt                                                                   <  1:2128238/140‑1 (MQ=255)
                                                                       aaTGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTc                                                                  <  1:4063818/140‑1 (MQ=255)
                                                                         tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATCAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  2:7637720/140‑1 (MQ=255)
                                                                         tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGTAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  1:5626950/140‑1 (MQ=255)
                                                                         tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGGAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  1:4196900/140‑1 (MQ=255)
                                                                         tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  1:8871665/140‑1 (MQ=255)
                                                                         tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  2:5276725/140‑1 (MQ=255)
                                                                         tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  1:4498556/140‑1 (MQ=255)
                                                                         tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  1:4941035/140‑1 (MQ=255)
                                                                         tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  2:8644076/140‑1 (MQ=255)
                                                                         tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  2:8044650/140‑1 (MQ=255)
                                                                         tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  1:943143/140‑1 (MQ=255)
                                                                         tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  1:7810795/140‑1 (MQ=255)
                                                                         tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTAATGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  2:8228128/140‑1 (MQ=255)
                                                                          gAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAcc                                                               <  2:6665804/140‑1 (MQ=255)
                                                                          gAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAcc                                                               >  1:1039982/1‑140 (MQ=255)
                                                                          gAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAcc                                                               >  1:2968646/1‑140 (MQ=255)
                                                                          gAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTGATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAcc                                                               <  2:9400672/140‑1 (MQ=255)
                                                                           aGGGGTGGTATGTTGCGAGCCAATGAAAAAAGAACGCCATCTTTATTGATGATTGATCCC‑CC‑GGAG‑G‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGCAGTAAGAGTTGTGAATGTCAAGTTCTTGGTCACCt                                                              <  2:6379557/140‑1 (MQ=255)
                                                                            ggggTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTg                                                             >  2:4623452/1‑140 (MQ=255)
                                                                               gtggtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTAt                                                          <  1:9329723/140‑1 (MQ=255)
                                                                                tggtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTAtt                                                         >  1:4716472/1‑140 (MQ=255)
                                                                                  gtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGTTTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTgg                                                       >  2:2454545/1‑140 (MQ=255)
                                                                                  gtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GGAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTgg                                                       >  1:6087804/1‑140 (MQ=255)
                                                                                  gtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAAt                                                                                                                 <  1:3937577/82‑1 (MQ=255)
                                                                                  gtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTgg                                                       >  1:3626646/1‑140 (MQ=255)
                                                                                  gtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTgg                                                       >  2:1930560/1‑140 (MQ=255)
                                                                                     tGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGa                                                    <  1:3415740/140‑1 (MQ=255)
                                                                                       ttGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACg                                                  >  2:7846097/1‑140 (MQ=255)
                                                                                         gCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGcc                                                <  2:6589525/140‑1 (MQ=255)
                                                                                         gCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGcc                                                <  2:6936782/140‑1 (MQ=255)
                                                                                          cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACTccc                                               >  2:8451221/1‑140 (MQ=255)
                                                                                            aGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTg                                             <  2:3783281/140‑1 (MQ=255)
                                                                                            aGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTg                                             <  2:2645428/140‑1 (MQ=255)
                                                                                             gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGa                                            >  2:5146946/1‑140 (MQ=255)
                                                                                             gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGa                                            >  1:1625967/1‑140 (MQ=255)
                                                                                             gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGa                                            >  1:10725174/1‑140 (MQ=255)
                                                                                             gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTAGTGGTGACGCCCTGa                                            >  2:2974009/1‑140 (MQ=255)
                                                                                             gCCAATTAAAAAAGAACGCCATAGATATTGTTGATAGATCCC‑AC‑GG‑TGG‑GG‑GG‑GACACATCGGGCCTAAATGGGCATGAAGTAATGGAGTATTAGATGTGAATGTCACTTTCTATGTCACCGGTATTGGTGACGCCCTGa                                            >  2:7290279/1‑140 (MQ=255)
                                                                                             gCCAAGTAAAAAAGAACGCCATAGTTATTGATGATTGATCCCGCG‑GG‑G‑G‑GG‑GG‑GAAGCATCGGGCATAAATGGGCATGTCGTTCTGGCGGATTAGGGGTGACTGTCAAGTTCGTTGTCACCGGGATTGGTGACGcccggc                                            >  2:10393347/1‑137 (MQ=255)
                                                                                              ccAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGaa                                           <  1:1854534/140‑1 (MQ=255)
                                                                                                 aTTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CCGGG‑G‑G‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATc                                        >  1:6445106/1‑140 (MQ=255)
                                                                                                  ttAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCa                                       >  2:5751690/1‑140 (MQ=255)
                                                                                                    aaaaaaGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAg                                     <  2:1309217/140‑1 (MQ=255)
                                                                                                      aaaaGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGaa                                   <  1:7530260/140‑1 (MQ=255)
                                                                                                        aaGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATg                                 <  1:5273412/140‑1 (MQ=255)
                                                                                                           aaCGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCa                              <  1:9239243/140‑1 (MQ=255)
                                                                                                             cGCCATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACg                            >  1:11231626/1‑140 (MQ=255)
                                                                                                             cGCCATATATATTGATGATTGATCCC‑CC‑GG‑T‑G‑GG‑GGTGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACg                            <  2:8331671/140‑1 (MQ=255)
                                                                                                                cATATTTATTGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACgaga                         <  1:5409450/140‑1 (MQ=255)
                                                                                                                  tatttattGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACt                       <  1:10128633/140‑1 (MQ=255)
                                                                                                                       attGATGATTGATCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGtg                  >  1:3057538/1‑140 (MQ=255)
                                                                                                                              attgatCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTAc           >  1:1235246/1‑140 (MQ=255)
                                                                                                                                  atCCC‑CC‑GG‑TGG‑GG‑GG‑GAAACATCGGGCATAAATGGGCCTGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGa       >  1:4385541/1‑140 (MQ=255)
                                                                                                                                               |                                                                                                                                         
AGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCC‑CC‑GG‑T‑G‑GG‑GG‑GAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAAC  >  NZ_CP025268/320470‑320743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑