BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,449,793	+T	pseudogene (3757/3779 nt)	tamB →	autotransporter assembly complex protein TamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,449,791	1	.	T	96.1%	2941.2 / 89.0	806	pseudogene (3755/3779 nt)	tamB	autotransporter assembly complex protein TamB
Reads supporting (aligned to +/- strand): ref base . (17/14); new base T (445/322); total (467/339)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 864 total aligned reads displayed.

GTCAGCGGCTATGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑A‑T‑TGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTAT  >  NZ_CP025268/4449652‑4449925

gTCAGCGGCTATGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑a                                                                                                                                          <  2:1046672/140‑1 (MQ=255)

   aGCGGCTATGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑A‑t‑tt                                                                                                                                     >  1:9457593/1‑139 (MQ=255)

   aGCGGCTATGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑A‑t‑tt                                                                                                                                     >  2:8510518/1‑139 (MQ=255)

    gCGGCTATGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑A‑t‑ttg                                                                                                                                    >  1:2993975/1‑138 (MQ=255)

    gCGGCTATGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑A‑t‑ttg                                                                                                                                    <  1:11159122/140‑3 (MQ=255)

          aTGTATTGCCAGGTCTGCAAGTGACATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTAt                                                                                                                               >  1:2085469/1‑140 (MQ=255)

          aTGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTAt                                                                                                                               >  2:3810596/1‑140 (MQ=255)

          aTGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTAt                                                                                                                               <  1:6060633/140‑1 (MQ=255)

           tGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATc                                                                                                                              >  1:5179299/1‑140 (MQ=255)

                     ggTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTCTATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGtt                                                                                                                    >  1:7405434/1‑140 (MQ=255)

                     ggTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGtt                                                                                                                    >  1:3837357/1‑140 (MQ=255)

                     ggTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGATCTATCAGTTCGAGtt                                                                                                                    >  2:3975867/1‑140 (MQ=255)

                      gTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGttt                                                                                                                   >  1:1537041/1‑140 (MQ=255)

                      gTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGttt                                                                                                                   <  1:1738792/140‑1 (MQ=255)

                         tGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTag                                                                                                                <  1:10319508/140‑2 (MQ=255)

                         tGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTag                                                                                                                >  1:4501087/1‑139 (MQ=255)

                               tGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGc                                                                                                          <  2:3475883/140‑1 (MQ=255)

                                     aCGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAatat                                                                                                    <  1:6125007/140‑1 (MQ=255)

                                     aCGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAatat                                                                                                    >  1:10615890/1‑140 (MQ=255)

                                       ggCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATAttt                                                                                                  <  1:5589666/140‑1 (MQ=255)

                                        gCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTg                                                                                                 >  1:8715980/1‑140 (MQ=255)

                                         cGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAAAGCGAATATTTGt                                                                                                >  1:6440424/1‑140 (MQ=255)

                                          gTGGGTATATTGGACTCTAGAGCAACACTCCCGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGAGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTc                                                                                               <  2:6396648/140‑1 (MQ=255)

                                            gggTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTa                                                                                             <  2:4268612/140‑1 (MQ=255)

                                             ggTATATTTGACTATATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTATACCAGGCAGTGG‑A‑TGGGATCTATATGATCGAGGTTTTGCAATGCGTCTATTTGTCTag                                                                                            >  2:7349609/1‑139 (MQ=255)

                                               tataTTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACgg                                                                                          <  2:2698197/140‑1 (MQ=255)

                                                   tttGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGt                                                                                      >  1:818175/1‑140 (MQ=255)

                                                       aCTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTAc                                                                                  >  1:7789726/1‑140 (MQ=255)

                                                        ctctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACg                                                                                 >  1:8365337/1‑140 (MQ=255)

                                                        ctctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACg                                                                                 >  2:10605955/1‑140 (MQ=255)

                                                        ctctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACg                                                                                 >  1:4025388/1‑140 (MQ=255)

                                                        ctctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACg                                                                                 >  1:2282728/1‑140 (MQ=255)

                                                          ctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGcc                                                                               <  1:5335078/140‑1 (MQ=255)

                                                          ctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGcc                                                                               <  2:5712703/140‑1 (MQ=255)

                                                           tataGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCca                                                                              >  1:6808625/1‑140 (MQ=255)

                                                           tataGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCca                                                                              <  1:2652711/140‑1 (MQ=255)

                                                             taGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCcaca                                                                            >  1:8074426/1‑140 (MQ=255)

                                                                   cacTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAg                                                                      >  2:5117053/1‑140 (MQ=255)

                                                                   cacTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAg                                                                      <  1:1295083/140‑1 (MQ=255)

                                                                   cacTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAg                                                                      >  2:3343379/1‑140 (MQ=255)

                                                                   cacTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAg                                                                      >  2:8838511/1‑140 (MQ=255)

                                                                     cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                    >  2:11176526/1‑140 (MQ=255)

                                                                     cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                    >  2:1185815/1‑140 (MQ=255)

                                                                     cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                    >  2:1626448/1‑140 (MQ=255)

                                                                     cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                    >  2:2200121/1‑140 (MQ=255)

                                                                     cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                    >  1:6685598/1‑140 (MQ=255)

                                                                     cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                    >  1:624544/1‑140 (MQ=255)

                                                                     cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGGTTACGCCACAAACAAGGc                                                                    >  2:11019252/1‑140 (MQ=255)

                                                                     cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTACGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                    >  2:9436286/1‑140 (MQ=255)

                                                                       cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCCCTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATAGTTGTCTACGGCAGTTTACGCCACAAACAAGGCac                                                                  >  2:6187940/1‑139 (MQ=255)

                                                                       cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                  <  2:4789103/140‑1 (MQ=255)

                                                                       cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                  <  1:365120/140‑1 (MQ=255)

                                                                        acgttacgttATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAAc                                                                 >  2:7172166/1‑140 (MQ=255)

                                                                        acgttacgttATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGC‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAAc                                                                 >  1:8430536/1‑140 (MQ=255)

                                                                          gttacgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGAACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGGTTACGCCACAAACAAGGCAAcgg                                                               >  2:10857255/1‑138 (MQ=255)

                                                                           ttacgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGt                                                              <  2:9779426/140‑1 (MQ=255)

                                                                           ttacgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGt                                                              <  1:2459299/140‑1 (MQ=255)

                                                                                 taTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTATATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTgg                                                        <  1:1869109/140‑1 (MQ=255)

                                                                                   tCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGAt                                                      <  1:72451/140‑1 (MQ=255)

                                                                                    cGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATg                                                     >  2:9238724/1‑140 (MQ=255)

                                                                                    cGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATg                                                     >  2:8279309/1‑140 (MQ=255)

                                                                                         gATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCat                                                >  1:314913/1‑139 (MQ=255)

                                                                                         gATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCaa                                                <  1:8855987/140‑1 (MQ=255)

                                                                                         gATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCaa                                                <  1:414721/140‑1 (MQ=255)

                                                                                             ccTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGcc                                            <  1:2808222/140‑1 (MQ=255)

                                                                                               tAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCa                                          >  1:1049153/1‑140 (MQ=255)

                                                                                                aaGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAg                                         >  2:4533520/1‑140 (MQ=255)

                                                                                                      tatCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAAGATTTGTCTACGGCAGTTTACGCCACAAACAAGGCACCAGTCACTGGAGGACCAATGCCCAGTTACTg                                   >  2:1384866/1‑140 (MQ=255)

                                                                                                       atCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTgg                                  >  2:8087813/1‑140 (MQ=255)

                                                                                                         cTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGc                                <  2:2043312/140‑1 (MQ=255)

                                                                                                         cTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGc                                <  2:7919274/140‑1 (MQ=255)

                                                                                                            gAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGAt                             >  2:10119544/1‑140 (MQ=255)

                                                                                                             aaGCCGTGTCTGGTGTAGACCAGTCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTAAGGCAGTTTACGCCACAAACAAGGCAACAGTAACTGGATGACCAATTCACAGTTAATGGGAGAtt                            >  2:2964835/1‑140 (MQ=255)

                                                                                                             aaGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGAtt                            >  1:3437179/1‑140 (MQ=255)

                                                                                                                ccGTGTCTGGTGTAGACCAGGCACTGGCT‑T‑TGCTCTCTCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGGTTACGCCCCAAACAAGGCACCAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCa                         >  2:6691936/1‑140 (MQ=255)

                                                                                                                ccGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATCTTTGTCTACGGCAGGTTACGCCACAAACAAGGCACCAGTCACTGGCTGACCAATGCCCAGTGACTGGGCGATGTCa                         >  2:2797634/1‑140 (MQ=255)

                                                                                                                    gtCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTAt                     <  1:5016589/140‑1 (MQ=255)

                                                                                                                    gtCTGGGGGAGCCCAGGCACTGG‑AGT‑TGCTCTATCAGTTCGAGAGTTAGCAATGCGAATACTAGTCCACGGCCGTTGACGCCACACACAAGGCAACAGTCACTGGATGACCAAGGCCCAGGTACTGGGCGATGTCAGTAt                     <  2:2477442/140‑1 (MQ=255)

                                                                                                                      cTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCg                   <  2:7544923/140‑1 (MQ=255)

                                                                                                                       tGGTGTAGACCTGGAACTGG‑ATT‑TTCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCTCTGGATGACAAATGCCCAGTTACTGGGCGATTTCAGTATCGa                  <  2:9023329/140‑1 (MQ=255)

                                                                                                                         gtgtAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATa                <  2:6065322/140‑1 (MQ=255)

                                                                                                                         gtgtAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATa                <  2:4971208/140‑1 (MQ=255)

                                                                                                                              gACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGTCCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc           <  1:7062623/140‑1 (MQ=255)

                                                                                                                              gACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc           <  1:8547024/140‑1 (MQ=255)

                                                                                                                              gACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc           <  1:5865521/140‑1 (MQ=255)

                                                                                                                              gACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATGTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc           <  2:5312583/140‑1 (MQ=255)

                                                                                                                               aCCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCTATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAcc          <  1:9088701/140‑1 (MQ=255)

                                                                                                                               aCCAGGCACTGG‑A‑TGTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCCCTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGGATCGATAACTAcc          >  2:865202/1‑140 (MQ=255)

                                                                                                                                ccAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCa         <  1:9238724/140‑1 (MQ=255)

                                                                                                                                ccAGGCACTGG‑A‑TGTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCa         <  1:6567756/140‑1 (MQ=255)

                                                                                                                                ccAGGCACAGGAA‑T‑TGCTCTCTCAGTTCGAGTTGTCGCAATGCGAATAATTGTCTACGGCAGTTGACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCa         <  2:7808855/140‑1 (MQ=255)

                                                                                                                                 cAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAg        <  1:10831403/140‑1 (MQ=255)

                                                                                                                                 cAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGAGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAg        <  2:6779970/140‑1 (MQ=255)

                                                                                                                                   ggCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGtt      >  1:472117/1‑140 (MQ=255)

                                                                                                                                   ggCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGtt      >  2:1853268/1‑140 (MQ=255)

                                                                                                                                    gCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTg     >  2:6996185/1‑140 (MQ=255)

                                                                                                                                    gCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGAGTTCAGTATCGATAACTACCAGTTg     >  1:10009714/1‑140 (MQ=255)

                                                                                                                                     cACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGt    <  1:11026205/140‑1 (MQ=255)

                                                                                                                                      aCTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGta   <  1:1182820/140‑1 (MQ=255)

                                                                                                                                      aCTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGta   <  1:9780506/140‑1 (MQ=255)

GTCAGCGGCTATGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑A‑T‑TGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTAT  >  NZ_CP025268/4449652‑4449925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`