BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,292,982	0	A	G	57.1%	65.9 / inf	323	noncoding (55/132 nt)	CXP41_RS06835	RtT sRNA
Reads supporting (aligned to +/- strand): ref base A (36/96); new base G (96/80); total (142/181)
Fisher's exact test for biased strand distribution p-value = 1.69e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 326 total aligned reads displayed.

CCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGC  >  NZ_CP025268/1292843‑1293115

cccGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCGGCGGCAACGCTCTCTCGCTGa                                                                                                                                        >  1:3867134/1‑140 (MQ=255)

   gcttGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGc                                                                                                                                     >  2:961314/4‑140 (MQ=255)

    cttGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCt                                                                                                                                    <  1:4843762/138‑1 (MQ=255)

    cttGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTGGCCTGCGGCAACGCTCTCTCGCTGACGCt                                                                                                                                    >  1:10414233/3‑140 (MQ=255)

    ctggggCATAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGGGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCt                                                                                                                                    <  1:2179035/137‑1 (MQ=255)

    cATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTGGCCTGCGGCAACGCGCTCTCGCTGGCGCt                                                                                                                                    >  1:935601/1‑140 (MQ=11)

     ttGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTc                                                                                                                                   >  1:10184784/2‑140 (MQ=255)

       gggCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCGTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                                 >  1:6534302/1‑140 (MQ=255)

        ggCCGACTATTGGCTCGCGGATTCGCTTGAGACTTCAGGGCCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAg                                                                                                                                <  2:6965262/140‑1 (MQ=11)

        ggCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAg                                                                                                                                >  1:179990/1‑140 (MQ=255)

        ggCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCGCGAg                                                                                                                                >  1:6176417/1‑140 (MQ=11)

         gCAGAATCTTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAgt                                                                                                                               >  2:11125557/1‑139 (MQ=11)

         gCAGAATATTTGATTGCGGATTCGCTAGAGAGTTCCGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTAGCTGGCGCTCGAgt                                                                                                                               <  2:5215722/140‑2 (MQ=11)

          cAGAATATTTGATTGCGGAGTCGTTTGAGAATTCCGGGGCTTTTGCAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGcc                                                                                                                              <  1:11024898/140‑1 (MQ=255)

               tatTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGACGGATTACTCAGAGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGAGGCAACGATCTCTCGCTGGCGCTCGAGTCGAccc                                                                                                                         >  1:7411653/1‑140 (MQ=11)

                  ttGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTa                                                                                                                      <  1:7597872/140‑1 (MQ=255)

                  ttGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTGGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGACGAACCTTa                                                                                                                      >  1:439115/1‑140 (MQ=255)

                  ttGATTGCGGACTCGCTTGAGAGTTCAGGGACTTTTGAAAGGGATGGGGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCttg                                                                                                                      >  2:4859716/1‑139 (MQ=11)

                    gATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTagt                                                                                                                    <  2:9263329/140‑3 (MQ=255)

                     agtGCGGATTCGCTTGAGAGTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCttggtc                                                                                                                   <  2:3162349/138‑5 (MQ=11)

                       tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTTAGCCCATCACACTTCTGAACGATGCCTGTGGCAAAGTTATCTCGCTTACTCTCGAGACGAACAATAGTCGa                                                                                                                 >  2:9155584/1‑140 (MQ=9)

                       tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                 <  2:7405714/140‑1 (MQ=255)

                       tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCACCGCTGCGCGCTTCGCCCAACGGGTCGATGCATGCGGCAACGCACACTCGCTGACGCTCGAGCCGAACCTTCGTCGa                                                                                                                 >  2:4178620/1‑140 (MQ=14)

                       tGCGGAGTCGCTTGAGCGTTCCGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGa                                                                                                                 <  2:2268830/140‑1 (MQ=11)

                        gCGGATTCGTTTGAGAATTCCGGGGATTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGaa                                                                                                                <  2:5390979/140‑1 (MQ=255)

                         cGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAg                                                                                                               >  2:6572103/1‑140 (MQ=14)

                            agtCGTTTGAGAATTCCGGGGCTTTTGCCAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCtt                                                                                                            <  2:4657754/138‑1 (MQ=14)

                              tCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTtct                                                                                                          <  2:6894843/140‑1 (MQ=255)

                                gtttGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCa                                                                                                        <  2:578916/138‑1 (MQ=255)

                                 tttGAGAATTCCGGGGCCGTTGAAAGTGATGGTGGTGTGGGAAGGATTAGTCTGCTCTGCGAGATTAGCATTTGTGTTAGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCAt                                                                                                       <  2:10334749/139‑1 (MQ=14)

                                 tctGAGCATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCAt                                                                                                       <  2:6437041/138‑1 (MQ=255)

                                   tGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGATCCGAACCTTAGTCGAAGCTTCTCAtac                                                                                                     >  1:8401792/1‑138 (MQ=255)

                                   tGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATcc                                                                                                     <  1:728408/140‑1 (MQ=255)

                                   tGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGACGAACCTTAGTCGAAGCTTCTCATcc                                                                                                     >  2:4026474/1‑140 (MQ=255)

                                   tGAGAATTCCGGGGATTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATcc                                                                                                     <  2:671716/140‑1 (MQ=255)

                                      gaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTc                                                                                                  <  1:1206549/140‑1 (MQ=255)

                                      gaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTc                                                                                                  <  1:5037102/140‑1 (MQ=255)

                                      caaTTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTc                                                                                                  <  1:10511791/139‑1 (MQ=255)

                                            cGGGGCTTTTGCAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCa                                                                                            <  1:7158200/140‑1 (MQ=25)

                                             ggggCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCA‑t                                                                                          <  1:9303862/140‑1 (MQ=21)

                                             ggggCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCA‑t                                                                                          <  2:1055827/140‑1 (MQ=21)

                                              gggCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGAGCTGCGCGCTTCGCCCTACGTGTCGTTGCATGTGGCAACGGTCTCTCGCTTACGCACGACACGAACCTTAGTCGACTCGTCTCAACCTTCCCCGctgt                                                                                          >  1:5869469/1‑137 (MQ=11)

                                                tcTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCA‑Tggg                                                                                       <  2:162259/139‑1 (MQ=12)

                                                tcTTTTGATAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCA‑Tggg                                                                                       <  1:10799870/139‑1 (MQ=12)

                                                gCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCTCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGATCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCA‑Tggg                                                                                       <  2:8491279/140‑1 (MQ=16)

                                                gCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCACTTCGGGTCGTTGCCTGCGTCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCA‑Tggg                                                                                       <  2:1189012/140‑1 (MQ=16)

                                                  ttttGTAAGTGATGGCGGTGGGGGAAGGATGACTCAGCGCGGCGCGCTTCGCCCGTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCa                                                                                     <  2:9701719/140‑1 (MQ=11)

                                                  ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCa                                                                                     <  2:3330638/140‑1 (MQ=17)

                                                  tattGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGAAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCa                                                                                     <  2:7270926/138‑1 (MQ=12)

                                                           tGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATCtttg                                                                            >  2:4414222/1‑139 (MQ=9)

                                                           tGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGACGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCCGAatatgtg                                                                            >  2:1771578/1‑137 (MQ=255)

                                                                         gAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGtt                                                              <  1:3437517/140‑1 (MQ=255)

                                                                          aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTg                                                             >  1:6461249/1‑140 (MQ=255)

                                                                           aGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGTCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCa                                                                                     >  2:10665509/1‑115 (MQ=255)

                                                                            ggATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGACGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTggg                                                           <  1:4026474/140‑1 (MQ=255)

                                                                            ggATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCT‑TGGGCAGAATATTTAATTGCGGATTCGTTggg                                                           <  1:8624460/140‑1 (MQ=11)

                                                                             gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGa                                                          <  1:6627014/140‑1 (MQ=255)

                                                                             gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGa                                                          <  1:249704/140‑1 (MQ=255)

                                                                                     aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAgg                                                  <  1:2970171/140‑1 (MQ=255)

                                                                                       cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAATCTTCTCATCCTTACCCGCA‑TGGGCAGAATATTTAATTGCGGATTAGTTGGGAAGTTCAGGGa                                                >  2:3016011/1‑140 (MQ=14)

                                                                                       cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCCGAATATTTAATTGCGGATTGGTTGGGAAGTTCAGGGa                                                >  2:5018569/1‑140 (MQ=14)

                                                                                       cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAgggg                                                >  2:2898477/1‑139 (MQ=255)

                                                                                       cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                >  2:10885847/1‑140 (MQ=255)

                                                                                       cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCACTCGCTGGCGCTCGAGTCGAACCGTGGGGGAAGCTTCTCAACCTTCCCCGCA‑TGGGGAGGATATTTAATTGCGGATTGGGTGGGGAGTTCAgggg                                                >  1:2859005/1‑139 (MQ=11)

                                                                                       cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTGGGTCGAAGCTTCTCATACTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGAGTCGGGGGGAAGTGCAGGGa                                                >  2:6793665/1‑140 (MQ=11)

                                                                                          tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACtt                                             >  2:6253641/1‑140 (MQ=255)

                                                                                            cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCGCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTGATTGCGGATTCGGTGGGAAGTTCAGGGACtttt                                           >  2:9851469/1‑140 (MQ=11)

                                                                                              cgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGa                                         <  1:6253641/140‑1 (MQ=255)

                                                                                               gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCCAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCT‑GGGGCAGAATATTTTATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaa                                        <  2:8069319/140‑1 (MQ=11)

                                                                                                cTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCT‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                       <  2:8901227/140‑1 (MQ=14)

                                                                                                 ttCGCCCTTCGGGTCGTTGCCTGCGGCATCGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAg                                      <  1:5942929/140‑1 (MQ=14)

                                                                                                 ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAg                                      <  1:11284743/140‑1 (MQ=255)

                                                                                                 ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGGAGAATATTTAATTGCGGTTTCGTTGGGAAGTTGAGGGTCGTTTGAAAg                                      >  1:10291082/1‑140 (MQ=14)

                                                                                                 ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGc                                                                                             <  1:9895487/86‑1 (MQ=18)

                                                                                                 ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGa                                                                                   >  2:3904052/1‑95 (MQ=18)

                                                                                                    gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGA                                   <  2:2531‑M1/140‑1 (MQ=255)

                                                                                                    gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGA                                   <  1:10676905‑M1/140‑1 (MQ=255)

                                                                                                      ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTTATTGCGGATTCGTTGGGCAGTTCAGGGACTTTTGAAAGTGATG                                 <  1:9968092‑M1/140‑1 (MQ=255)

                                                                                                      ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATG                                 <  1:1471509‑M1/140‑1 (MQ=255)

                                                                                                         tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGTGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGTTGGTG                              >  2:8316648‑M1/1‑140 (MQ=255)

                                                                                                          cGGGTCGGTGCCTGCGGCAACGCTCGCGCGCTGACGCTCGAGCCGAACCGTCGTCGAAGCTTCTCAACCTTCCCCGCC‑TGGGCAGAATACTTGATTGCGGAGTCGCTTGAGCGTTCAGGGACTTTTGCAAGTGAtggtgg                             <  2:6137930/140‑1 (MQ=255)

                                                                                                           gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCa                                                                                                        <  2:11254408/65‑1 (MQ=25)

                                                                                                                gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGGGTCGCACCGTGGGCGAAGCTTCTCATCCGTCCCCGCC‑TGGGCCGGATATTGATTTTGGGATTCGTTGGGAAGGTCAGGGACGTTTGAAAGTGATGGTGGTggggg                       >  2:4092411/1‑140 (MQ=9)

                                                                                                                 ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGA                      <  1:2370260‑M1/140‑1 (MQ=255)

                                                                                                                 gtgCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCT‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGA                      <  2:2717322‑M1/139‑1 (MQ=255)

                                                                                                                  tGCCTGCGGCACCGCTCTCTCGCTGGCGCTCTAGTCGCACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAA                     <  2:9512362‑M1/140‑1 (MQ=255)

                                                                                                                  tGCCTGCGGCAACGCTCTCTCGCTTGCGCTCGACTCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCGCAGTGGGC‑CAATATATAATTGCGGTATGAGTGTGACGTTCAGGGACTTTGGAAAGTGATGGGGGGGGGGGac                     >  2:3763697/1‑139 (MQ=2)

                                                                                                                  tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAA                     <  1:11172404‑M1/140‑1 (MQ=255)

                                                                                                                  tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑GGGGCAGAATATTTAATTGCGGATTCGGTGGGAAGTTCAGGGTCTTTGGAAAGTGATGGTGGTgggggca                     >  2:10226301/1‑138 (MQ=11)

                                                                                                                         ggCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcg              <  1:1534589‑M1/140‑3 (MQ=255)

                                                                                                                         ggCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTACCCGCT‑TGGGCAGAATATTTAATTGCGGAGTCGTTGGGAATTTCATGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcg              <  2:3603719‑M1/140‑3 (MQ=255)

                                                                                                                           cAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCC‑TGGGCCGAATATTTAATTGCGGATTCGTTGGGTAGTTCAGGGACTGTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaa            >  2:5570502‑M1/1‑136 (MQ=255)

                                                                                                                           cAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCT‑TGGGCAGAATATTTGATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaa            <  1:10012918‑M1/140‑5 (MQ=255)

                                                                                                                              cGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAAGTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaacct         >  1:10263286‑M1/1‑133 (MQ=255)

                                                                                                                              cGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaacct         >  2:3741079‑M1/1‑133 (MQ=255)

                                                                                                                                 tctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcg      <  2:7548962‑M1/140‑11 (MQ=255)

                                                                                                                                 tctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcg      <  2:9425016‑M1/140‑11 (MQ=255)

                                                                                                                                   tctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATGTAATTGCGGAGTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGGGGTGGGGGAAGGATTcgaaccgtcgta    >  1:9000483‑M1/1‑128 (MQ=255)

                                                                                                                                    ctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTTATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaag   <  1:5597620‑M1/140‑14 (MQ=255)

                                                                                                                                     tcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGTTGGTGGTGGGGGAAGGATTcgaaccttcgtagt  >  2:7641923‑M1/1‑126 (MQ=255)

CCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGC  >  NZ_CP025268/1292843‑1293115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`