BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,181,292	0	G	T	24.1%	276.2 / 43.5	187	intergenic (‑51/+257)	gatY/fbaB	tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
Reads supporting (aligned to +/- strand): ref base G (51/91); new base T (38/7); total (89/98)
Fisher's exact test for biased strand distribution p-value = 1.20e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 191 total aligned reads displayed.

GTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATA  >  NZ_CP025268/2181153‑2181430

  ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtt                                                                                                                                             <  2:9090697/140‑1 (MQ=255)

  gtCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtt                                                                                                                                             <  2:749024/139‑1 (MQ=255)

   tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGTAAATATTTGttt                                                                                                                                            >  1:3063235/1‑140 (MQ=255)

   tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATtttttt                                                                                                                                            >  2:2555179/1‑140 (MQ=255)

    cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtttt                                                                                                                                           <  1:10509628/140‑1 (MQ=255)

    cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtttt                                                                                                                                           <  1:6349912/140‑1 (MQ=255)

      agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                                         <  1:5430780/140‑1 (MQ=255)

          tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAata                                                                                                                                     <  1:8273360/140‑1 (MQ=255)

           gtgAATATTTAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAatat                                                                                                                                    <  2:1372734/140‑1 (MQ=255)

           gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGTTTTCAAAATATAGTGCAATTATTTGTTTTTAAatat                                                                                                                                    >  1:8781435/1‑140 (MQ=255)

           gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAatat                                                                                                                                    <  2:6421327/140‑1 (MQ=255)

           gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAatat                                                                                                                                    <  2:7298989/140‑1 (MQ=255)

           gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAatat                                                                                                                                    >  2:8760114/1‑140 (MQ=255)

           gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAatat                                                                                                                                    <  2:1478738/140‑1 (MQ=255)

           gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGTTTTAAAAATATAATGTAATTATTTGTTTTTAAatat                                                                                                                                    >  1:1668668/1‑140 (MQ=255)

                    gAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAc                                                                                                                           <  2:4188096/140‑1 (MQ=255)

                     aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACg                                                                                                                          >  2:7856720/1‑140 (MQ=255)

                      aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGa                                                                                                                         >  2:5120108/1‑140 (MQ=255)

                      aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAAGa                                                                                                                         >  2:7689826/1‑140 (MQ=255)

                       tGCCGTAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATACCGAt                                                                                                                        <  2:5783178/140‑1 (MQ=255)

                        gCCGTAACCGCATAACCGCCGCGATGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACTTAAATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGAT‑c                                                                                                                      <  2:2040018/140‑1 (MQ=255)

                        gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGACATTATTTGTTTTTAAATATCGAGATAACGAT‑c                                                                                                                      <  2:8303372/140‑1 (MQ=255)

                                  cATACCCGCCGCGCTGTGGGTTGTTCAGCATCTGCTTTGTCGATACCACTTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑a                                                                                                           <  1:2771961/140‑1 (MQ=255)

                                  cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTAGTCGATACCACGTACAGTTTCATAGCCTGACGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACTAT‑CACAAAAACG‑a                                                                                                           <  2:7797821/140‑1 (MQ=255)

                                   aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACTTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAATTATCGAGATAACGAT‑CACAAAAACG‑Ac                                                                                                          <  1:1491750/140‑1 (MQ=255)

                                   aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAATAATTAAATTATTTTTTTTTAAATATAGAGATAACGAT‑AACAAAAACG‑Ac                                                                                                          >  1:4237294/1‑140 (MQ=255)

                                          ccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCCAAATATTATGTAATTATTTGGTTTTTAATATCGAGATTACGAT‑CACAAAAACG‑CCAAGATGa                                                                                                   >  1:465297/1‑140 (MQ=255)

                                           cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAAAGAT‑CACAAAAACG‑ACAATATGaa                                                                                                  >  1:2917132/1‑140 (MQ=255)

                                            gcgcTGTGCGTTGTTCATCATCTGCTTTGTCGATACCACGTACATTTTCATATCCGGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCTAGATAACGAT‑CACAAACACG‑ACAATATGaaa                                                                                                 <  1:369252/140‑1 (MQ=255)

                                            gcgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGaaa                                                                                                 <  2:2364503/140‑1 (MQ=255)

                                                 gtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAattat                                                                                            <  2:8189814/140‑1 (MQ=255)

                                                  tgCGTTGGTCAGCATCTGCTTTGTCGATACCACGTACAGTTACATATCCTGTCGTTTGGATTCGATTGCAAAAAATAATGAAAAAATTTGTTTTTAAATAACGAGAAAACGAA‑AACAAAAAAG‑ACAAAAAGAAAataatt                                                                                           >  1:5673431/1‑140 (MQ=255)

                                                    cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCg                                                                                         <  2:4634306/140‑1 (MQ=255)

                                                    cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAAATATTTTTTTTTAAATATAGAGATAACGAT‑CACAAAAAAGCACAAAAT‑AAAattattag                                                                                         >  1:418317/1‑138 (MQ=255)

                                                      ttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCgag                                                                                       <  1:1206332/140‑1 (MQ=255)

                                                       tgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGTTTTCAAAATATAATGAAATTATTTGTTGTTTAATATCGAGATAACGAT‑CACAAAAACG‑TCAATATGAAAATTATTCgagg                                                                                      >  1:7670118/1‑140 (MQ=255)

                                                        gttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTATATATCGAGATAACGAT‑CAAACAAACG‑ACAATATGAAAATTATTGgaggg                                                                                     >  1:7135856/1‑139 (MQ=255)

                                                        gttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATTAAATTATTTTTTTTTAAATATAGAGATAAAGAT‑AAAAAAAACG‑ACAATATGAAAATTATTCgagga                                                                                     >  1:524151/1‑140 (MQ=255)

                                                         ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATTTCCTGTCGTGTGTTTTCGATTTCAAAATATAATTCAATTATTTGTTTTTTAATATGGAGATTACGAT‑CACAAAAACG‑TCAATTTGGAAATTATTAgaggag                                                                                    >  1:10706518/1‑140 (MQ=255)

                                                         ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTGGATTTCAAAAAATAATTAAAATAATTTTTTTTTAATATATAGATAAAGAT‑AAAAAAAACG‑ACAAAATTAAAatttttattggag                                                                                    >  1:3373593/1‑133 (MQ=255)

                                                         ttCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTTTGTTTGCGATTTCAAAATATAATGAAATTATTTGTTTTGAAATATCGAGAGAACGAG‑CACAACAACG‑ACAATATGAGAATTATTCgaggag                                                                                    >  2:11250559/1‑140 (MQ=255)

                                                            aGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAATTTAATTATTTTTTTTTTAAAAACTATATAAAGATAAACAAAAAC‑‑ACAAAATGAAAATTATTAGAGGAGTgt                                                                                 >  1:9670305/1‑139 (MQ=255)

                                                            aGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGa                                                                                 >  2:10857372/1‑140 (MQ=255)

                                                            aGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGa                                                                                 >  2:2646598/1‑140 (MQ=255)

                                                            aGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCAGTCGTTTGTTTTAGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATAAGAAAAATAATAGAGGAGTGa                                                                                 >  2:9459680/1‑140 (MQ=255)

                                                              cATCTGCTTTGTCTATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTGATTTTAAATATCGATATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGaaa                                                                               <  2:5180083/140‑1 (MQ=255)

                                                                   gCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATTAAGAT‑GACAAAAACG‑ACAAAATGTAAAATATTGGAGGTGTGAAAGGC‑aa                                                                         >  1:7299072/1‑140 (MQ=255)

                                                                   gCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGAATTAAAAAAATAATTAAATTATTTTTTTTTTAAAATAGAGATAAAGAT‑CACAAAAAAC‑ACAAAATGAAAAATATTCTAGGTGTGAAAGTC‑aa                                                                         >  1:10552427/1‑140 (MQ=255)

                                                                   gCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTGTTCGATATCAAAATATAAAGAAATTATTTTTTTTTAAAAATCGAGAAAACCAA‑AACCAAAAAG‑ACCATATTCAAATTTTTCTATTAGTGAAAGGC‑aa                                                                         >  1:957121/1‑140 (MQ=255)

                                                                        gTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACCAT‑CACAAAAACG‑ACAAAATTAAAATTATTCGAGGAGTGAAAGGC‑aaaaaaa                                                                    >  2:4899473/1‑140 (MQ=255)

                                                                        gTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATAAAATGAAAATATTTTTTTTTAAATATAGATATAAAGAT‑AACAAAAACG‑ACAAAATGTAAATTATTAGATGAGTGAAATGC‑aaaaaaa                                                                    >  1:4464406/1‑140 (MQ=255)

                                                                             tcccACTTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTTTTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGcc                                                               <  1:2779524/138‑1 (MQ=255)

                                                                             taacACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGcc                                                               <  2:9407469/137‑1 (MQ=255)

                                                                             tACCACGTACTTTTTCATCTCCTGTGGTTTGTTTTCGATTTCAAAATATTTTTAATTTATTTGTTTTTAATTATCGTTATAACGAT‑AACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGTAAGGC‑AAAAAAACGGcc                                                               <  1:9893318/140‑1 (MQ=255)

                                                                             tACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGcc                                                               <  2:3451266/140‑1 (MQ=255)

                                                                             tACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGcc                                                               <  2:6850784/140‑1 (MQ=255)

                                                                              aCCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCt                                                              <  1:11039365/140‑1 (MQ=255)

                                                                              aCCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTAAAAATATAATGAAAATATTTTTTTTTTAATATAGAGATCAAGAT‑ATAAAAAACT‑ACAATATGATAATTATTGGGGGGGTGTTAGGG‑GATAAAAAGGCCt                                                              >  1:8740315/1‑140 (MQ=255)

                                                                                 aagTACATTTTCATATCCTGTCGTTTGTTTTCGTTTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTccc                                                           <  2:4871397/138‑1 (MQ=255)

                                                                                  cGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCg                                                          >  2:187945/1‑140 (MQ=255)

                                                                                   gTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAATGAAAATATTTTTTTTTAAATATAGAGATAACGAT‑AACAAAAAAG‑ACAATATTAAAATTATTCGATGAGTGAAATGC‑AAAAAAACGGCCtaacgt                                                         >  1:1962806/1‑135 (MQ=255)

                                                                                   gTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGa                                                         >  2:1582773/1‑140 (MQ=255)

                                                                                   gTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAAAG‑ACAAAATGAAAATTATTCGTGGAGTGTAAGGC‑AAAAAAACGGACTCCCGa                                                         >  2:3282747/1‑140 (MQ=255)

                                                                                    tACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGAt                                                        <  2:11189633/140‑1 (MQ=255)

                                                                                       aTTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATTTCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAgg                                                     <  1:11162658/140‑1 (MQ=255)

                                                                                        ttttCATATCCGGTCGTTTGTTGTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAggg                                                    <  1:8077046/140‑1 (MQ=255)

                                                                                         tttCATATCCTGTCGTTTGTTTTCTTTTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACTAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGa                                                   <  1:9648456/140‑1 (MQ=255)

                                                                                           tCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAg                                                 <  2:10534897/140‑1 (MQ=255)

                                                                                           tCATATCCTGTCGTTTGTTTTCTATTTAAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAg                                                 <  1:6098196/140‑1 (MQ=255)

                                                                                           tCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAg                                                 <  2:9670305/140‑1 (MQ=255)

                                                                                                 ccTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAATGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAg                                           >  2:4850534/1‑140 (MQ=255)

                                                                                                     tCGTTTGTTTTAGATTTCAAAATATAATGTAATTATTTTTTTTTAAATATAGAGATAACGAA‑CACAAAAACG‑ACAATATGAAAAATATTCGAGGAGTGAAAGGC‑AAAAAAAAGGACTACCGATAGGGAATCCGTAGCaaa                                       >  2:2860184/1‑140 (MQ=255)

                                                                                                     tCGTTTGTTTTAGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAAAGAT‑AACAAAAACG‑ACAATATTAAAATTATTCGATGAGTGAAAGGC‑AAAAAAAAAGACTACCGATAGGGAATCCGTAGCaaa                                       >  2:621016/1‑140 (MQ=255)

                                                                                                       gtttgtttTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACTAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAATGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGt                                     <  1:8166882/140‑1 (MQ=255)

                                                                                                            ttttCGATTTCAAAATATAATCAAATTATTTGTTTTTAAATATCGATCTAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGcgcg                                <  1:10110446/140‑1 (MQ=255)

                                                                                                              ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtg                              <  2:10585967/140‑1 (MQ=255)

                                                                                                                   tttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt                         <  1:4681702/140‑1 (MQ=255)

                                                                                                                             tactGAAATTATTTGTTTTTAAATATCGAGATCACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg               <  1:9123862/137‑1 (MQ=255)

                                                                                                                             taATTAAATTATTTGTTTTAAAATATCGAGAAAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg               <  1:2559514/140‑1 (MQ=255)

                                                                                                                             taATGAAATTATTTGTTTTTAAATATCGAGATTACGAA‑CAAAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg               <  2:3601996/140‑1 (MQ=255)

                                                                                                                             taATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACACAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg               <  2:3410572/140‑1 (MQ=255)

                                                                                                                             taATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg               >  2:3262957/1‑140 (MQ=255)

                                                                                                                             taATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg               <  1:1855991/140‑1 (MQ=255)

                                                                                                                             taATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg               <  1:1856673/140‑1 (MQ=255)

                                                                                                                             taATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg               <  2:8457743/140‑1 (MQ=255)

                                                                                                                             taATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg               <  2:10842383/140‑1 (MQ=255)

                                                                                                                             taATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg               >  2:740259/1‑140 (MQ=255)

                                                                                                                             taATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg               <  2:4267378/140‑1 (MQ=255)

                                                                                                                             taATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg               <  2:3180025/140‑1 (MQ=255)

                                                                                                                             aaattaaattaTTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg               <  1:6576981/139‑1 (MQ=255)

                                                                                                                             aaattaaattaTTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg               <  2:7191623/139‑1 (MQ=255)

                                                                                                                              aaTGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg               <  1:1810260/140‑1 (MQ=255)

                                                                                                                                tGAAATTATTTGTTTTTAAATATCTAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg            <  1:8991957/140‑1 (MQ=255)

                                                                                                                                tGAAATTATTTGTAATAAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg            <  1:9441862/140‑1 (MQ=255)

                                                                                                                                  aaaTTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg          <  2:4149773/140‑1 (MQ=255)

                                                                                                                                     ttattTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa       <  1:402959/140‑1 (MQ=255)

                                                                                                                                     ttattTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa       <  1:240967/140‑1 (MQ=255)

                                                                                                                                     ttattTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa       <  2:2048182/140‑1 (MQ=255)

                                                                                                                                     ttattTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa       <  1:10251095/140‑1 (MQ=255)

                                                                                                                                       attTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAAc     <  1:5795744/140‑1 (MQ=255)

GTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACG‑ACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATA  >  NZ_CP025268/2181153‑2181430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`