Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,233,2390TA29.0% 313.4 / 43.4 179intergenic (+139/+234)yohP/dusCsmall membrane protein YohP/tRNA dihydrouridine(16) synthase DusC
Reads supporting (aligned to +/- strand):  ref base T (39/81);  new base A (49/0);  total (96/83)
Fisher's exact test for biased strand distribution p-value = 1.88e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 181 total aligned reads displayed.

AATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTT  >  NZ_CP025268/2233101‑2233378
                                                                                                                                            |                                                                                                                                             
aattaattAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTg                                                                                                                                              <  2:3953716/140‑1 (MQ=255)
     attaatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGATCCTGTCATGTGCATTGCTTCATTTAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCATTCGCCGCCGCCCCATG‑CAccc                                                                                                                                        <  1:8421271/140‑1 (MQ=255)
              atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGG‑GGGGGGCGGGCCAAAGGCC‑CCCCCCCCCC‑CAcccccccgcgct                                                                                                                               >  1:3550650/1‑133 (MQ=255)
              atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCGGGGGGGG‑GGCCAAACCCCCCCCCCCCCGC‑CCCCCCCCGGcgc                                                                                                                                >  1:4332616/1‑138 (MQ=37)
              atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑CGGGGGC‑GGCCCAACGCCGCCCCCCCCAG‑CCCCCCCCGGcgca                                                                                                                               >  2:1157496/1‑137 (MQ=37)
               tcaGGTCCGAAAATAACGAGAATATTTCCGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCACTCGCCGCCCCCCCCAG‑CACCCCCCGGCTCTg                                                                                                                              >  2:3681295/1‑140 (MQ=37)
               tcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑CGGGGGC‑GGCCCAAAGCCGCCCCCCCCAC‑CACCCCCCGGctccg                                                                                                                              >  1:9391770/1‑138 (MQ=37)
                    tCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCCAACGCCGCCCCCCCCAG‑CACCCCCCGGCGCAGGCgca                                                                                                                         >  2:5123347/1‑138 (MQ=25)
                    tCCGAAAATAACGAGAATATGTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGG‑AGGGGGG‑GGCCAATAGCCGCCGCCCCCAG‑CCACCCCCGGCGATGGCGaa                                                                                                                         >  1:2901497/1‑140 (MQ=25)
                     ccGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAAc                                                                                                                        <  2:966365/140‑1 (MQ=255)
                      cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGG‑CGGGGGG‑GGCCCAACGCCCCCCCCCCCCC‑CCCCCCCCCGCGCAGGCGGACa                                                                                                                       >  1:1461460/1‑140 (MQ=25)
                      cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGTGGGG‑GGCCCATAGCCGCCGCCCCCAGCCACCCCCGGG‑TATTGCGAACa                                                                                                                       >  2:3113297/1‑140 (MQ=25)
                      cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGGCCAACGCCGCCGCCCCCAG‑CACCCCCGGGCGCTGGCGGACa                                                                                                                       >  1:8600471/1‑140 (MQ=25)
                        aaaaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGG‑AGGGGGG‑GGGCCAGACCCGCCGCCCCCAC‑CACCCCCGGGGTCTGGGGGACaaa                                                                                                                     >  1:6329397/1‑140 (MQ=255)
                          aaTAACGAGAAGATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGTGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAAt                                                                                                                   <  2:6536578/140‑1 (MQ=255)
                             aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑GGGGGGG‑GGGCCAACGACGCCCCCCCCCC‑CACCCCCCGCCGCGGGGGCACaaaaaaga                                                                                                                >  1:344708/1‑136 (MQ=25)
                             aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCCAACGCCGCCGCCCCCAG‑CACCCCCGGGCTCTGGCGAACAAAATcgc                                                                                                                >  2:4666963/1‑140 (MQ=255)
                               cGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCTTGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATcgccg                                                                                                              <  1:277138/140‑1 (MQ=255)
                               cGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATcgccg                                                                                                              <  2:1749757/140‑1 (MQ=255)
                                    atttttCTGTCTCTCATCCTGTTGCGCGCCTGTCATGTGCATTGCTTCCTAACATCCCTGGCGCACGGAGCGCGC‑CGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAACTCGCCGCTgcg                                                                                                         <  1:264283/137‑1 (MQ=18)
                                      atctCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTACATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGcgcg                                                                                                       <  2:7320210/137‑1 (MQ=255)
                                      atTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGcgcg                                                                                                       <  2:8486523/140‑1 (MQ=255)
                                       tttCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCgg                                                                                                      <  2:4193834/140‑1 (MQ=255)
                                       tttCAGTCTCTCATCCTGTTGCGCTCCTGTCAGGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCACTCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACACAAGCGCCGCTGCGCgg                                                                                                      <  2:4572875/140‑1 (MQ=255)
                                            gTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGccc                                                                                                 <  1:6045749/140‑1 (MQ=255)
                                            gTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGccc                                                                                                 <  1:1849007/140‑1 (MQ=255)
                                             tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCCCTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAAGCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCt                                                                                                <  1:9950338/140‑1 (MQ=255)
                                             tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCt                                                                                                <  1:7381245/140‑1 (MQ=255)
                                             tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCt                                                                                                <  2:865147/140‑1 (MQ=255)
                                              ctcacATCCTGTTGCGCTCCTGTCAGGTGCATGCCTTCCTATCACCACGGGCGCAACCAGCGCGC‑ACCGGGC‑GGCCAACCGCCGCCGCCCCCGT‑CACCCCCGGGCTCCGGCGAAAAAACTCGCCGCAGCTCGGTGCCCTc                                                                                               <  2:8707758/140‑1 (MQ=255)
                                               tctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AAGGGGC‑GGCCAAAAGCCGCCCCCCCCAG‑CACCCCCCGGCGCAGGCGAAAAAAAAAGCCCCAGCGCGGttgccacg                                                                                              >  1:10250669/1‑133 (MQ=18)
                                                ctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGACGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCgg                                                                                             >  2:7523377/1‑140 (MQ=255)
                                                 tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCCCCCCCAG‑CACCCCCGGGCTCAGGCGAACAAAATCGCCGCGGCCCCGTGCCCTCGGc                                                                                            >  2:2872249/1‑140 (MQ=18)
                                                 tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATAGCCGCCGCCCCCTG‑CACCCCCCGGCACTGGCGAACAAAAAAGCCGCTGCGCGGTGCCCACGGc                                                                                            >  2:4645177/1‑140 (MQ=18)
                                                       tGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAAACCCCGCCGCCCCCAG‑CACCCCCGGGCACTGGCGAACAAAATCGCAGCTGCGCGGTGCCCAAGGCTTaaac                                                                                      >  2:7199328/1‑137 (MQ=18)
                                                       tGTTGCGCTACTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGAAGCGCGC‑ATGGGTC‑GGACCAAAACACCCACCCCCAA‑CAACCCCCGGCAAATGCGAACAAAAAAGCCGCTGCGCGGTGACCTACGCtttaac                                                                                      >  2:5852362/1‑136 (MQ=255)
                                                           gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑CGGGGGC‑GGCCAAACGCCCCCGCCCCCAG‑CCCCCCCCGGCACTGGCGAACAAAAAACCCGCAGCGCGGTGCCCACCGCGAATCCCATa                                                                                  >  2:7987376/1‑140 (MQ=17)
                                                            cgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTAc                                                                                 <  1:539804/140‑1 (MQ=255)
                                                            cgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTAc                                                                                 <  1:8811325/140‑1 (MQ=255)
                                                              cTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCAG‑CACCCCCGGGCGCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCGTATCCCGTAAgg                                                                               >  2:1837642/1‑140 (MQ=18)
                                                                ccTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGCGCGCGC‑AGGGGGC‑GGCCAAGCGCCGCCGCCCCCTG‑CCCCCCCGGGCTCTGGCGAACAAAATCGCCGCGGCGCGGTGCCCACGGCGTAAACCATACGGC‑t                                                                            >  2:6517075/1‑140 (MQ=17)
                                                                ccTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAAACGCCGCCGCCCCCAG‑CACCCCCCGGCTCTTGCGAACAAAAAACCCGCGGCGCGGTGCCCGCGGCGTATCCCGTAAgggt                                                                             >  2:8985366/1‑138 (MQ=17)
                                                                   gTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TAcc                                                                         <  1:882105/140‑1 (MQ=255)
                                                                   gTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TAcc                                                                         <  2:1117245/140‑1 (MQ=255)
                                                                   gTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TAcc                                                                         <  1:3339883/140‑1 (MQ=255)
                                                                         tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGG‑AGGGGGC‑GGGCCATAGCCGCCGCCCCCAC‑CACCCCCCGGCGAATGCGAACAAAAACGCCGCGGCGCGGGGGCCTCGGCTTTTACCAGTCGGC‑TACCCGGtgg                                                                   >  2:4169346/1‑138 (MQ=9)
                                                                         tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCCGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCATACGGC‑TACCGGGTCg                                                                   >  2:9556280/1‑140 (MQ=255)
                                                                           cATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCCCCGCCCCCCCCAG‑CACCCCCCCGCTCAGGCGAACAAAATCCCCCCAGCGCGGTGCCCACGGCTTATACCCAACGGC‑TACCCGGTCggg                                                                 >  2:4686575/1‑140 (MQ=14)
                                                                           cATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAAACCCCGCCCCCCCCAG‑CACCCCCCGGCTCTGGCGAACAAAAACGCCGCAGCGCGGTGCCCTCGGCTTATCCCATAAGGC‑TACCCGGTCggg                                                                 >  2:9946483/1‑140 (MQ=14)
                                                                             ttGCTTCATATACTCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGgcg                                                               <  2:4385760/140‑1 (MQ=255)
                                                                             ttGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑CCGGGGC‑GGGCAAACTCCCCCCCCCCCAC‑CACCCCCCGGCTCTCGCGAACAAAAAACCCCCAGCGCGGTGCCCACGGCGTATACCCTACCGCAAACCGGGACgggg                                                                >  2:3281215/1‑139 (MQ=9)
                                                                              tGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑CGGGGGC‑GGCCACAAGCCGCCCACCCCAC‑CACCCCCCGGCGCTTGCGAACAAAAACGCCCCGGCGCGGTGGCCTCCGGGTATGCCAGCCCGG‑GACCGGGTCGGgcgc                                                              >  2:1388024/1‑140 (MQ=9)
                                                                              tGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGGCCAACGCCCCCGCCCCCTG‑CAACCCCCGGCGATGGCGAACAAAATAGCCGCTGCGCGGTGCCCGCGGCGGATCCCTTACGGG‑GACCCGGGCGGgggc                                                              >  2:6973905/1‑140 (MQ=14)
                                                                              tGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCAG‑CACCCCCCGGCTCTGGCGAACAAAAACGCCGCTGCGCGGTGGCCACGGCTTTAACCATACCGC‑AACCGGGTAGGgcgc                                                              >  2:8163258/1‑140 (MQ=14)
                                                                               gCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGcgcg                                                             <  2:10974991/140‑1 (MQ=255)
                                                                                   cATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGGCAATAGCGGGCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGGGCGGTGCCCTCGGCGGATACCTTACCGC‑AGCCGAGGAGGGCGCGAggg                                                         >  2:11052020/1‑139 (MQ=14)
                                                                                     tataATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAATACGCCGCGGCGCGGTGCCCACGGCTTATCCCTTACGGC‑TACCGGGTCGGGGGCGGGGTaa                                                       >  2:10279091/1‑140 (MQ=11)
                                                                                     tataATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTaa                                                       <  2:2971921/140‑1 (MQ=37)
                                                                                     tagactCACGGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTTCCCTCGGCTTATCCCTTACGGC‑TAACGGGTCGGGCGCGAGGTaa                                                       <  2:7852129/135‑1 (MQ=11)
                                                                                       taATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACa                                                     <  1:10326629/140‑1 (MQ=35)
                                                                                       taATCACTGGCGAATGGAGCGCGC‑TGGGGGC‑GGCCAAGCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACa                                                     <  1:2263740/140‑1 (MQ=255)
                                                                                        aaTCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACAt                                                    <  2:3824551/140‑1 (MQ=34)
                                                                                         aTCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATc                                                   <  1:6742372/140‑1 (MQ=33)
                                                                                         aTCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATc                                                   <  2:6672537/140‑1 (MQ=33)
                                                                                          tCACTGGCGCAAGGAGCGCGC‑ACGGGGC‑GGCCAATCGCCCCCCCCCCCAT‑CACCCCCCGGCACAGGCGAACAAAAAACCCCCAGCCCGGTGCCCACGGCATAACCCGTAAGGC‑AACCCGGTCGGGCGCGAGGTAACaaac                                                  >  2:4860991/1‑137 (MQ=2)
                                                                                            aCTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATACCCGCCGCCCCCAG‑CACCCCCCGGCGCAGGCGAAAAAAAAACCCGCGGCGCCGTGCCCACCGCGTATACCATAAAGC‑TACCCGGGCGGGGGCGGGGGAACAAccca                                                >  2:1322985/1‑139 (MQ=2)
                                                                                              tGGCGCAAGGAGCGCGC‑AGGGGGG‑GGCCAATCGCCGCCCCCCCCAG‑CACCCCCGGGGTCTGGCGAACCAAATAGCCGCGGCGCGGTGGCCACGGCGTATACCATACGGC‑TAACGGGTCGGGGGGGTGGTAACATACCAGt                                              >  2:8795187/1‑140 (MQ=2)
                                                                                                gcgcAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTaa                                            <  1:7533668/140‑1 (MQ=32)
                                                                                                    acggAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCGGGCGAACAAAATCGCCGCTGCGCGGGGCCCTCGGCTTCTCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCGGTAAAAcg                                        <  2:2718293/138‑1 (MQ=11)
                                                                                                      ggAGCGCGC‑AGGGGGC‑GGCCCATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGACCCAAATCGCCGCGGCGCGGTGCCCTCGGCGTATCCCGTACGGC‑TACCCGGTCGGGCGCGGGGGAACATCCCAGTAAAAcgcg                                      >  2:11134053/1‑140 (MQ=11)
                                                                                                        aGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGcc                                    >  2:9457562/1‑140 (MQ=32)
                                                                                                        aGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGcc                                    <  1:7249703/140‑1 (MQ=32)
                                                                                                          cgcgc‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                  <  2:516509/140‑1 (MQ=32)
                                                                                                          cgcgc‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                  <  2:6459106/140‑1 (MQ=32)
                                                                                                          cgcgc‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                  <  2:9013900/140‑1 (MQ=32)
                                                                                                          cgcgc‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                  <  1:882883/140‑1 (MQ=32)
                                                                                                          cgcgc‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                  <  2:9223986/140‑1 (MQ=32)
                                                                                                                aGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc                             <  1:7135133/140‑1 (MQ=32)
                                                                                                                aGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc                             <  1:11213231/140‑1 (MQ=32)
                                                                                                                aGGGGGC‑GGCCAAGCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc                             <  1:2167370/140‑1 (MQ=17)
                                                                                                                         gcCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATccc                     >  2:7932797/1‑139 (MQ=17)
                                                                                                                         gcCAATCGCCGCCGCCCCCAG‑CACCCCCGGGCTCTGGCGAAAAAAATAGCCGATTCGCGGTGCCCTCGGCTTATACCTTAAGGC‑TAACGGGTAGGTCGCGAGGTAACAACACATTAAAACGCGCCCTCAGCCCACATCCa                     >  1:426357/1‑140 (MQ=11)
                                                                                                                           cAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtg                   <  2:6313672/140‑1 (MQ=32)
                                                                                                                             aTCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTACCATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg                 >  2:9228376/1‑140 (MQ=17)
                                                                                                                             aTCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTACCATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg                 >  1:3003328/1‑140 (MQ=17)
                                                                                                                             aTCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCTCAGCCAtgtg                 >  2:5417079/1‑140 (MQ=17)
                                                                                                                             aTCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg                 >  2:157119/1‑140 (MQ=32)
                                                                                                                             aTCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg                 >  2:2198455/1‑140 (MQ=32)
                                                                                                                             aTCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg                 >  1:6456630/1‑140 (MQ=32)
                                                                                                                             aTCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg                 >  2:6809726/1‑140 (MQ=32)
                                                                                                                             aTCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg                 >  1:9509376/1‑140 (MQ=32)
                                                                                                                               cgccgccgcCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTggg               >  1:2543651/1‑140 (MQ=32)
                                                                                                                               cgccgccgcCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTggg               >  1:6533369/1‑140 (MQ=32)
                                                                                                                                gccgccgccCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGc              >  2:8338612/1‑140 (MQ=32)
                                                                                                                                   gccgccCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGc           >  2:11063935/1‑140 (MQ=32)
                                                                                                                                    ccgccCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAATCGCGCCCTCAGCCCACATCCATGTGGGCTGcc          >  1:1058882/1‑140 (MQ=17)
                                                                                                                                    ccgccCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGcc          <  2:4549047/140‑1 (MQ=32)
                                                                                                                                      gccCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGcccc        <  1:7979404/140‑1 (MQ=32)
                                                                                                                                        ccccTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCgg      >  1:978201/1‑140 (MQ=32)
                                                                                                                                            tG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCtt  >  2:10600692/1‑140 (MQ=32)
                                                                                                                                            |                                                                                                                                             
AATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGC‑AGGGGGC‑GGCCAATCGCCGCCGCCCCCTG‑CACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGC‑TACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTT  >  NZ_CP025268/2233101‑2233378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑