Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252684,414,7050TG22.4% 1097.4 / 84.9 492V131V (GTT→GTG) yjfMDUF1190 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (114/268);  new base G (107/3);  total (221/271)
Fisher's exact test for biased strand distribution p-value = 3.55e-41
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 500 total aligned reads displayed.

ACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCA  >  NZ_CP025268/4414566‑4414844
                                                                                                                                           |                                                                                                                                             
 cATTTGCGCGCGTGGCTGGAACAACGCCAAAACGACATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTCAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGttt                                                                                                                                              >  2:4444374/1‑140 (MQ=255)
        gcgcgTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑tt                                                                                                                                      <  1:6874746/140‑1 (MQ=255)
         cgcgTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑ttt                                                                                                                                     >  2:3275866/1‑140 (MQ=255)
           cgTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGGTTCTGGA‑ttttt                                                                                                                                   >  2:5886328/1‑140 (MQ=255)
                  ggAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGc                                                                                                                            <  1:5816346/140‑1 (MQ=255)
                        acGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGACggggt                                                                                                                      >  1:10702804/1‑138 (MQ=255)
                         cGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTGTGGA‑TTTTTGTTTAGGCGGGttt                                                                                                                     >  1:8358763/1‑139 (MQ=255)
                         cGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTa                                                                                                                     >  2:4150800/1‑140 (MQ=255)
                         cGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTa                                                                                                                     <  1:10564825/140‑1 (MQ=255)
                         cGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCGGGA‑TTGTTGTTAATCCggggtt                                                                                                                     >  2:8039134/1‑137 (MQ=255)
                         cGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTGCTGGG‑TTTTTGTTAATCCGGGttt                                                                                                                     >  1:2326218/1‑139 (MQ=255)
                         cGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTGCTGGA‑TTTTTTTTAATCCggggtg                                                                                                                     >  1:5615154/1‑137 (MQ=255)
                         cGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGA‑TTTTTGTTAAGACGGGttt                                                                                                                     >  1:5506508/1‑139 (MQ=255)
                         cGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGGC‑TTTTTGTTAATCCggggga                                                                                                                     >  1:7660299/1‑137 (MQ=255)
                         cGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGGA‑TTTTTTTTAAGCCGGGGTa                                                                                                                     >  2:1111757/1‑140 (MQ=255)
                         cGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGGA‑TTTTTGTTAAGCCGGGTTa                                                                                                                     >  2:6394780/1‑140 (MQ=255)
                          gCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCAGGA‑TTTTTGTTAAGCCGGGGTAt                                                                                                                    >  2:5497865/1‑140 (MQ=255)
                              aaaCGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCCGGTTATTCGt                                                                                                                >  2:8684479/1‑140 (MQ=255)
                               aaCGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTa                                                                                                               <  1:9361541/140‑1 (MQ=255)
                               aaCGGCATTTTAGGCCGTTGTTCCGAAGCATATGAATCATCAGAACTGTCAGTCTAAGTACGCATATAGCTACTACGACAATGTTGAACTCAGTGGGATCCCGGTCGTTTCTGGA‑ATTTTGTTTAGCCGGGTTATTCGTa                                                                                                               <  2:7571164/140‑1 (MQ=255)
                                   gCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGa                                                                                                           <  1:7464564/140‑1 (MQ=255)
                                   gCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGa                                                                                                           <  2:10382134/140‑1 (MQ=255)
                                    cATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGGGGGGA‑TTTTTTTTTATGAGGGGTATTGGTAAAAAt                                                                                                          >  1:5013530/1‑140 (MQ=255)
                                     aTTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATc                                                                                                         <  2:6494758/140‑1 (MQ=255)
                                          aTGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGAt                                                                                                    <  1:1373321/140‑1 (MQ=255)
                                           tGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGATACTACTACAATGTTGAACAGAGTTGGATCACGGTCGTTTCTGGA‑TTTTTGTTAAGACTGGTTATTAGTAAATATCGCGATg                                                                                                   >  2:10150384/1‑140 (MQ=255)
                                            gCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGa                                                                                                  <  1:2560638/140‑1 (MQ=255)
                                              cGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATAGCGATGAGc                                                                                                >  2:4474763/1‑140 (MQ=255)
                                               gATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTATGGA‑TTTTTTTTTATCCGGGTTTTTAGTAAATATAGCGCTGAGcc                                                                                               >  1:398944/1‑140 (MQ=255)
                                                aTGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGGA‑TTTTTGTTACGACGGGTGAGTCGTTAAGATCGCGTTGTGCC‑g                                                                                             >  2:6253047/1‑140 (MQ=255)
                                                 tGTTCCGAAGAATATGACTCAGCAGAACTGTCATTCTAAGTACGAAAATTGCTCCTACGACAATGTAGAACAGAGTTGGATCCCGGGCGTGTCTGGA‑TTTTTATTTACCCGGGATAGTAGTAAAGATGGCGAGGGGCC‑gg                                                                                            >  2:3960153/1‑139 (MQ=255)
                                                 tGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑gt                                                                                            <  1:1799425/140‑1 (MQ=255)
                                                 tGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑gt                                                                                            <  1:1978212/140‑1 (MQ=255)
                                                 tGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑gt                                                                                            <  2:1832641/140‑1 (MQ=255)
                                                 tGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCGAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCAGGA‑TTGTTGGTAAGCCGGGGGAGTCGTAAAGATCGCGATGAGCC‑gg                                                                                            >  2:3388354/1‑139 (MQ=255)
                                                 cgctCCGAAGAATATGACTCCGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑gt                                                                                            <  1:8810898/137‑1 (MQ=255)
                                                      cGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑gtttgtt                                                                                       <  2:763978/140‑1 (MQ=255)
                                                         agaaTATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTtat                                                                                    <  1:9972265/140‑1 (MQ=255)
                                                             tatGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTGGGGA‑TTTTTTTTTAGCCGGGTTTTTAGTAAAAAAAGCGGTGATCC‑GTGTTTTtattaaa                                                                                >  1:4597915/1‑136 (MQ=255)
                                                             tatGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACa                                                                                >  2:6615266/1‑140 (MQ=255)
                                                             tatGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGGGTGGA‑TTTTTGTTAAGCCGGGTGTTTCGGAAAGATCGCCGTGAGCC‑GGTTGTTTTTaaaa                                                                                >  1:370205/1‑138 (MQ=255)
                                                                gACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGGATTTTTTGTTAA‑CCGGGGTTTTCGTAAATTTAGCGATGAGCC‑GGTTGTTTATTACAgcg                                                                             >  1:4776798/1‑140 (MQ=255)
                                                                  cgcagcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAgcggc                                                                           <  2:10780120/138‑1 (MQ=255)
                                                                     agcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGt                                                                        <  1:5387347/140‑1 (MQ=255)
                                                                     agcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGG‑TTTTTGTTATTCCGGGTTATTAGGGAAGATAGCGATTTGGC‑GTTTGTGTATTACAGcggcggg                                                                        >  1:6955267/1‑139 (MQ=255)
                                                                     agcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTAGGGA‑TTTTTGGTAATCCGGGGTGTTAGGAAAGATCGCGATGATCC‑GGGTGTTTATAAAAGCCGCGGt                                                                        >  1:2938666/1‑140 (MQ=255)
                                                                     agcacAACTTACAGTCTAAGTACTAACAATGAGACTACGACAATGTTTCACACAGTGTGATCCCGGACGGTTCTGGA‑TATGTGTAAATCCGTGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGAGTATAAGAGCGGCGGt                                                                        <  2:7076703/140‑1 (MQ=255)
                                                                        agAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTcc                                                                     <  2:3583800/140‑1 (MQ=255)
                                                                          aaCTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAAc                                                                                 >  2:7841815/1‑126 (MQ=255)
                                                                            cTGTCAGTCTAAGTACGAAAATTGCTACTACGACACTGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCtt                                                                 <  2:160686/140‑1 (MQ=255)
                                                                            cTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGG‑TTTTTTTTAAGCCGGGGTTTTAGTAAATATAGCGGTGATCCGGTTTGTTT‑TAAAATCGGCGGTTGCTCtt                                                                 >  1:8022542/1‑140 (MQ=255)
                                                                             tGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCttt                                                                <  1:383085/140‑1 (MQ=255)
                                                                               tCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCCGGTTATTAGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCACTTTTg                                                              >  2:5139901/1‑140 (MQ=255)
                                                                               tCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGGG‑TTTTTGTTAATCCGGGGTATTAGTAAAGATAGCGATTAGCC‑GGTTGTTTTTAAAAGCGGCGGGTGCTCTTTTg                                                              >  1:4476943/1‑140 (MQ=255)
                                                                                 aGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGGTAAGCCGGGGTATTCGTAAAGATCGCGGTGAGCC‑GTTTGTTTATAAAAGCGGCGGGTCCGCGTTTGCt                                                            >  2:5703148/1‑140 (MQ=255)
                                                                                 aGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCt                                                            >  2:8474205/1‑140 (MQ=255)
                                                                                      aaGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTcgcg                                                       <  1:8213135/140‑1 (MQ=255)
                                                                                       aGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCgcgc                                                      <  2:2343339/140‑1 (MQ=255)
                                                                                       aGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGA‑TTTTTTTTTATCCCGGGTATTAGTTAATATAGCGATGTGGC‑GGTTTTGTTTAAAATCCGCGGGTGCTCTTTTTTTTGgcgc                                                      >  1:7824789/1‑140 (MQ=255)
                                                                                        gTACGCAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGcc                                                     <  1:7939017/140‑1 (MQ=255)
                                                                                        gTACGAAAATTTCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTGTGCTTCGCGcc                                                     <  2:8344525/140‑1 (MQ=255)
                                                                                        gTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGcc                                                     <  1:3093467/140‑1 (MQ=255)
                                                                                        gTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGcc                                                     <  1:348528/140‑1 (MQ=255)
                                                                                        ctACGAACAGCGCCACGACCACAATGGCGACCAGAGTTGGATCCCGGTCGAGTCTGGA‑ATTTTGTGAAGCCGGGTTATTCGTAACGTACGCGCTGAGCC‑GTTTGTTAATACCAGCGGCGGTTCCTCTTTTGCTGCGCGcc                                                     <  2:9013967/139‑1 (MQ=255)
                                                                                            gAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGt                                                 <  2:7318243/140‑1 (MQ=255)
                                                                                             aaaaTTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTc                                                <  1:10030836/140‑1 (MQ=255)
                                                                                             aaaaTTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCGGGA‑TTTTTTTTAATCCCGGTTATTGGTAAAGATCCCGTTTTTCC‑GTTTGTTTATAACAGCGGCGGTTGCTCATTTGCCTCGCGCCCAggg                                                >  2:4132872/1‑138 (MQ=255)
                                                                                                aTTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTgg                                             <  1:7168333/140‑1 (MQ=255)
                                                                                                 ttGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGGA‑TTTTTTTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GGTTGTTTATAACAGCGGCGGTTACTCGTTTGCTTCGCGCCCAGTCTGgc                                            >  2:1119612/1‑140 (MQ=255)
                                                                                                     tactacGACAATGTTGACCAGAGTTGGATCCCGGTCGTTTCTGTA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCgcag                                        <  2:9761849/140‑1 (MQ=255)
                                                                                                     tactacGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCgcag                                        <  2:8807539/140‑1 (MQ=255)
                                                                                                     tactacGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCgcag                                        <  1:1467253/140‑1 (MQ=255)
                                                                                                     tactacGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCgcag                                        <  1:976640/140‑1 (MQ=255)
                                                                                                     tactacGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCgcag                                        <  1:9042800/140‑1 (MQ=255)
                                                                                                      actacGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCgcagc                                       <  1:2872634/140‑1 (MQ=255)
                                                                                                      actacGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCgcagc                                       <  1:4150800/140‑1 (MQ=255)
                                                                                                       ctacGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCgcagca                                      <  1:7055681/140‑1 (MQ=255)
                                                                                                          cGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTGTGGT‑TTTTTTTTAATGCGGGGTATTGGTTAAGATAGCGCTGATCCGGTTTGTTTTTAA‑AGCCGCGGGTACTCTTTTGCTTAGCGCCCAGTCTGGCGCAGCAcat                                   >  1:8569539/1‑138 (MQ=255)
                                                                                                          cGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACtt                                   <  2:1337283/140‑1 (MQ=255)
                                                                                                            aCAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCt                                 <  2:9326319/140‑1 (MQ=255)
                                                                                                            aCAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCt                                 <  1:5225661/140‑1 (MQ=255)
                                                                                                            aCAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCt                                 <  1:6629034/140‑1 (MQ=255)
                                                                                                             cAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTg                                <  1:6094563/140‑1 (MQ=255)
                                                                                                                tGTTGAACAGAGTTGGATCCCGGTGGTTTCTGGA‑TTTTTGTTTAGCCGGGGTATTCGTAAAGTTCGCGTTTTGCC‑GTTTGTTTATAACCGCGGCGGTTGCTCTGTTGAATCGCGCCCAGGCTGGCGCAGCACGTCTgggg                             >  2:2872497/1‑138 (MQ=255)
                                                                                                                  ttGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAt                           <  1:8380360/140‑1 (MQ=255)
                                                                                                                  ttGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAt                           <  2:4638067/140‑1 (MQ=255)
                                                                                                                  ttGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAt                           <  2:9405318/140‑1 (MQ=255)
                                                                                                                      aCAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACt                       <  2:1957765/140‑1 (MQ=255)
                                                                                                                          agTTGGATCCCGGTCGTTTCTGGT‑TTTTTTTTTAGCCGGGGTTTTAGGAAAGATCGCGATGTGCC‑GGTTTTTTTTAACAGCGGCGGGTCCACTTTTGGTTGGCGCCCAGTCTGGCGGAGCACATCGGGGGTTTACTCCTg                   >  1:10362741/1‑140 (MQ=255)
                                                                                                                          agTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAATCCGGGTTATTCGTAAATATAGCGATTAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACGTCTGGTGATTACTCCTg                   >  2:10940715/1‑140 (MQ=255)
                                                                                                                          agTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAATCCGGGTTATTAGTAAAGATCGCGCTGATCC‑GGTTTTTTATAAAAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTccag                   >  1:9521548/1‑138 (MQ=255)
                                                                                                                          agTTGGATCCCGGTCGTTTATGGA‑ATTTTTTTAATCCGGGTTATTAGTAAAAATCGCGATGAGCC‑GTTTTTTTATAAAAGCGGCGGTTGCTGTTTTGGTTCGCGCCCAGTATTGCGCAGCACTTATTGTGATTAAACCTg                   >  2:2457918/1‑140 (MQ=255)
                                                                                                                          agTTGGATCCCGGTCGTGTATGGA‑TTTTTTTTTAACCGGGGTATTAGTAAATAAAGCGATGATCC‑CTTTTTTTATAAAAACGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTTGGGCAGCCCTTCTGGTGATTaaaacag                   >  2:3124247/1‑134 (MQ=255)
                                                                                                                            ttGGATCCCGGTCGTTTGTGGA‑TTTTTTTTTATCCGGGTTATTCGTAAAGATCGCGATGTGCC‑GTGTTTTTTTAAAAGCGGGGGTTGCAGTTTTGGGTGGCGCCCCGTCTGGCGCAGCACTTCTGGTGTTTAATCCgggg                 >  1:11188500/1‑139 (MQ=255)
                                                                                                                                    cGGTCGTTTCTGGA‑TTTTTTTTAAGCCCGGTTATTAGTAAATATAGCGATTAGCC‑GTTTGTTTATAACAGCGGCGGTTACAATTTTTAATAGCGCCCAGTCTGGCGCAGACAATCTGGTGATTAAACCAGGCGCTCCGGt         >  2:6973871/1‑140 (MQ=255)
                                                                                                                                    cGGTCGTTTCTGGA‑TTTTTGTTAAGACGGGTTAATCGTAAAGTACGCGTTGAGCC‑GTTTGTTTATAACAGCGGCGGTTACTCTTTTGCTTCGCGCCCCGTCGGGGGCAGCTCTTCGGGGGATTACGCCGGGCGCTCCggg         >  2:4825059/1‑139 (MQ=255)
                                                                                                                                    cGGTCGTTGCTGGA‑TTTTTTTTAAGCCGGGGTATTCGTAAAGATCGCCATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTTCTTCGCGCCCAGTCTGGCGCCGCACTTCAGGTGATTACACCGGGCGCTCCGGt         >  2:9544594/1‑140 (MQ=255)
                                                                                                                                      gTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCTATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTc       <  1:3237562/140‑1 (MQ=255)
                                                                                                                                      gTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTc       <  2:1057950/140‑1 (MQ=255)
                                                                                                                                          tttCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGATTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGc   <  1:6426317/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                             
ACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGA‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCC‑GTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCA  >  NZ_CP025268/4414566‑4414844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑