Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268164,4120AC29.1% 301.4 / 35.6 233intergenic (+47/‑149)hrpB/mrcBATP‑dependent helicase HrpB/bifunctional glycosyl transferase/transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (69/94);  new base C (10/57);  total (81/152)
Fisher's exact test for biased strand distribution p-value = 5.80e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 238 total aligned reads displayed.

CAAAAAGAGATGAAAGGGCGTTATCCCAAA‑CATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCG  >  NZ_CP025268/164273‑164550
                                                                                                                                            |                                                                                                                                          
cAAAAAGAGATGAAAGGGCGTTATCCCAAA‑CATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCa                                                                                                                                            >  2:9906343/1‑140 (MQ=255)
cAAAAAGAGATGAAAGGGCGTTATCCCAAA‑CATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCa                                                                                                                                            >  1:3719530/1‑140 (MQ=255)
        ttttaaaGGTCGTTATCCCAAA‑CATGTCTGGCCGGACGCCCCGGCAAATACTGCACCGACGCGACGGACGAAAAATTATTCTTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgcg                                                                                                                                    <  2:7159548/136‑1 (MQ=38)
        gaTGAATGGGCGTTATCCCAAA‑CATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTTTTCGTAAGGTTGGTTTTCTCCCTCTCCGTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgcg                                                                                                                                    <  2:11210783/140‑1 (MQ=255)
        gaTGAAAGGGCGTTATCCCAAA‑CTTTTCTGGCCGGACGACCCGGAAATTACTGCACCGACGCTACGGACGAAAAAGTATTCGTAAGGTTGTTTTTCTCCCTCTCCCTGTGGTAGAGGGCCGGGGTGAGGGCATCAgcgcg                                                                                                                                    <  2:10570490/140‑1 (MQ=38)
        gaTGAAAGGGCGTTATCCCAAA‑CATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGTGGGCATCAgcgc                                                                                                                                     <  2:6665682/139‑1 (MQ=255)
        gaTGAAAGGGCGTTATCCCAAA‑CATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgcg                                                                                                                                    >  2:1779575/1‑140 (MQ=255)
        gaTGAAAGGGCGTTATCCCAAA‑CATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgc                                                                                                                                     >  1:6665682/1‑139 (MQ=255)
        catgcacgggCGTCACCCCAAA‑CATGTCCGCCGGTACCACCCGCCAAAACCTGCACCGCGGCGACGTCCGACAAAGTAGTCGTAAGGTTGGTTTTCTCCCTCTCCCGGTGGGAGCGGGCCGGGGTGAGGGCCTCAgcgcg                                                                                                                                    <  2:9310734/133‑1 (MQ=255)
          tGAAAGGGCGTTATCCCAAA‑CATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCa                                                                                                                                  <  2:9071731/140‑1 (MQ=255)
          ataaaGGGCGTTATCCCAAA‑CATGTCGGGCCGGAGGACCCGGCAAATACGGCACCGACGCTCCGGACGAAAAAGTATTCGTAAGTTTGGTTTTCTCCCTCTCCCTGTGGGATTGGGCCGGGGTGCGGGCATCAGCGCGCa                                                                                                                                  <  2:4676271/138‑1 (MQ=37)
             aaGGGCTTTATCCCAAA‑CTTGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTATGGTTGGTTTTCGCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGt                                                                                                                               <  2:10674343/140‑1 (MQ=255)
              aGGGCGTTATCCCAAA‑CATGTGGGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGTGGGCATCAGCGCGCACGtt                                                                                                                              <  2:6525333/140‑1 (MQ=255)
              aGGGCGTTATCCCAAA‑CATGTCGGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTTTTCGTAAGGTTGGTTTTCTCCCTCGCCCTGGGGGTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGtt                                                                                                                              <  2:6779897/140‑1 (MQ=38)
                 gCGTTATCCCAAA‑CATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGtggtc                                                                                                                           >  1:7706426/1‑136 (MQ=255)
                   gTTATCCCAAA‑CATGTCGGGCCGGACGCCCCGGAAAAACCGGCACCGACGGGACGTACGAAAAATTATTCGTATGGTTTGTTTTCTCCCTCACCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTtcaccc                                                                                                                         <  2:11139051/140‑1 (MQ=37)
                   gTTATCCAAAA‑ATTGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccc                                                                                                                         <  2:3890620/140‑1 (MQ=255)
                    ttATCCCAAA‑CTTGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAATGTATGCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                                        <  2:10823079/140‑1 (MQ=255)
                    ttATCCCAAA‑CTGGTCTGCCCGGCCGACCCGGCAATTACTGCACGGCCGCGAGGGACTAACCTCTAATCGTAAGGTTGGTATTCTCCCTCTCCCAGGGGGTGAGGGCCGGGGTGAGGGCCTCAGCGCGGACGTtcaccct                                                                                                                        <  2:9547300/140‑1 (MQ=255)
                    ttATCCCAAA‑CATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                                        >  2:5451307/1‑140 (MQ=255)
                    ttATCCCAAA‑CATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGAGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTgcaccca                                                                                                                        >  1:5237601/1‑139 (MQ=255)
                    ttATCCCAAA‑CATGTCTGGCCGGACGACCCGGCAAAAACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGATAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTtcaccct                                                                                                                        <  2:5656709/140‑1 (MQ=255)
                    ttATCCCAAA‑CATGTCTGGCCGGACGACCCGGAAAATACTGCACCGACGCTCCGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGGGGGAGTGGGCCGGGGTGTGGGCCTCAGCGCGCACGTtcaccct                                                                                                                        <  2:1665298/140‑1 (MQ=255)
                    ttATCCCAAA‑CATGTCTGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGGACTAAAAATTATTCGTAAGGTTGGTTTTCTCCCTCCCCTTGTGGTAGTGGGGCGGGGTGTGGGCATCAGCGCGCACGTtcaccct                                                                                                                        <  2:1334584/140‑1 (MQ=255)
                    ttATCCAAAA‑CTTGTGTGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGGACTAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCACCCTGTGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTtcaccct                                                                                                                        <  2:583245/140‑1 (MQ=38)
                    ttACCCCAAA‑CTGTTCTGGCCGGCCGCCCCGGCAACTCCTCCACCGCCGCGCGGGACTAAAATTTTTGTGTATGGTGGGTTTTCGCCCCCTCCCTGTGGGTGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTtcaccct                                                                                                                        <  2:2413025/140‑1 (MQ=255)
                    ttACCCCAAA‑CAGGTCTGGCGGGACGACCCGCCACATACTGCACCGACGCGACTGACTAAAAAGTATTCGTAAGGTGGGTTTGCCCCCACGCCCTGTGGGTGAGGCCCGGGGTGAGGGCCTCAGCGCGCACGTtcaccct                                                                                                                        <  2:6991224/140‑1 (MQ=255)
                    tcaccccACA‑CATGACTGGCCGGACGACCCGGCAAAACCTGCACCGACGCTACTGACGAAAACGTAGACCTAACGTTGGATTTCACCCCCGCCCGGGGGGAGAGGGCCGGGGTGACGGCATCAGCGCGCACGTtcaccct                                                                                                                        <  2:8425708/136‑1 (MQ=255)
                     caacccAAACCATGTCTGCCCGGACGCCCCGGAAAATCCTGCCCCCACGCGACGGACTAAAAAGTATTCGTACGGTTGGTGTTCTCCCACCCCCAGGGGGATAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                                        <  2:10628490/137‑1 (MQ=255)
                        cccAAA‑CATGTCTGGCCGGACGACCCGGCACATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGTTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctcacc                                                                                                                    <  2:10473377/140‑1 (MQ=255)
                           caaccttGTCGGGCGGGCCGCCCCGGAAAATCCGGCCCCGCCGCGACGGACGAAAAATTTTTCGAAAGGTTGGTTTTCTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTcaccctcaccct                                                                                                                  <  1:2715938/134‑1 (MQ=21)
                                ctgtgtgGCCGTACGCCCCGGAAAATACTCCACCGACGCGACGGACTAAAAATTTTTCGTACGTTTGGTTTTCTCCCTCTCCCTGTGGGTGTGGGCCGGGGGGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAAccc                                                                                                             <  1:1627289/139‑1 (MQ=25)
                                aTGTCTGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGGACTAAAATGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGGGGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAccc                                                                                                             <  1:8546239/140‑1 (MQ=38)
                                  gTCTGGCCGTACGACCCGGCAAATACGGCACCGACTCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCGTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                           <  2:6382918/140‑1 (MQ=39)
                                  gTCTGGCCGGACGACCCGTCAAATACTGCACCGACGCGACGGACGAAAACGTATTCGTAAGGTTGGTTTACTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                           <  2:8556121/140‑1 (MQ=255)
                                  gTCTGGCCGGACGACCCGGCAAATACTGCCCCGCCGCGACGGACGAAAATGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGGGGGAGAGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                           <  2:2876174/140‑1 (MQ=38)
                                  gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAATAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGAACCACACCCAAACCctc                                                                                                           >  1:8392073/1‑140 (MQ=37)
                                  gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTGACCCACACCCGCACCctc                                                                                                           >  1:6344342/1‑140 (MQ=37)
                                  gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCACACCCAAAaccac                                                                                                           >  1:3252305/1‑138 (MQ=37)
                                  gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCCCCCCCACCCGAAcccac                                                                                                           >  1:7531561/1‑138 (MQ=38)
                                  gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCTCACCCAAAcccac                                                                                                           >  1:8583363/1‑138 (MQ=255)
                                  gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTGCCCCACCCCCGAAcccac                                                                                                           >  1:8987395/1‑138 (MQ=37)
                                  gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGGCCCCGCACCCGAAcccac                                                                                                           >  1:9979187/1‑138 (MQ=37)
                                  gTCGGGCGGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGTAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                           <  2:5845961/140‑1 (MQ=255)
                                   tCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTAAACCTAAACctct                                                                                                          >  2:10383284/1‑140 (MQ=255)
                                    gtGGCCGGACCACCCGCAAAATACGGCCCCGACGCTACGCACTAAAAATTATTCGTATGGTGGGTTTTCTCCCACGCCCTGGGGGCGAGGGCCGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCtctc                                                                                                         <  2:2060561/139‑1 (MQ=255)
                                    cgggCCGGACTACCCGGCAAATCCTGCACCGACGCGACGGACGCAAATGTATTCGTACGGTTGGTTTTCTCACTCTCCCTGTGGGTGAGGGCCGGGGTGAGGGCTTCAGCGCGCACGTTCACGCTCACCCTAACCCtctc                                                                                                         <  2:10376821/138‑1 (MQ=38)
                                       gcgggACGACCCGCAAATTACTGCACGGACGCGAGGGACGAAATAGTATTCGTATGGTGGGTTTTCTCCCGCTCCCTGTGGTAGTGGGCGGGGGTGGGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCt                                                                                                      <  2:4459499/137‑1 (MQ=255)
                                       gccggccgCCCCGGCAAAGACGGCCCCGACGCGACGGACGAAAAAGTATGCGAATGGTTGGTTTACTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCt                                                                                                      <  1:7420048/140‑1 (MQ=37)
                                       gCCGGACGCCCCGTCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGTTTGTCTCCCTCTCCCTGTGGGAGAGGGCCGGGTTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCt                                                                                                      <  2:6184380/140‑1 (MQ=38)
                                         cGGCCGACCCGCAAAATACGGCCCCGACGCGAGGCACTAAAAATTATTCTTATGGTGGTTTTTCTCCCTCGCCATGTGGGAGTGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCa                                                                                                    <  1:594406/140‑1 (MQ=255)
                                         cGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCa                                                                                                    <  2:4882118/140‑1 (MQ=255)
                                          ggACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCGCCGCGCGCACGTGGACCCACCCCCCAACCCAATCCCGCac                                                                                                   >  1:4214455/1‑139 (MQ=37)
                                           gacgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGTTCACCCACACCCGAACCCTCTCCCTCacg                                                                                                  >  2:6835297/1‑138 (MQ=255)
                                           gacgacCCGGAAAAACCTGCACCGACGCTCCGCACGAAACAGATTCCGTACGGTTGGTTTTCACCCTCCCCCGGTGGGTGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg                                                                                                  <  2:5330669/140‑1 (MQ=255)
                                           gacgacCCGCAAAATACTGCACCGACGCGACGTACGAAAAATTTTTCGTATGTTTGGTTTTCGCCCTCTCCGGGGGGGTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg                                                                                                  <  1:10205570/140‑1 (MQ=255)
                                            acgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGTGCCGCACCCCAACCCACACCCCCCAgg                                                                                                 >  1:1982532/1‑140 (MQ=25)
                                               ccccGGCAAAGACGGCACCGACGCGACGGACGAAAAAGAATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCCGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag                                                                                              <  2:9012834/139‑1 (MQ=38)
                                               acCCGGCAAATACTGCCCCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag                                                                                              <  2:4800362/140‑1 (MQ=255)
                                                 ccGTCAAATACTGCCCCGACGCGTCGGACGATAAAGTATTCTTATTGTTGGTTTTCTCCCTCGCCCTGTGGTAGTGGGCCGGGGTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag                                                                                            <  2:6045597/140‑1 (MQ=255)
                                                 ccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCCCGTGCACCCTCACCCGTACCCGCTCCCGCCAGGGagag                                                                                            >  2:9405875/1‑140 (MQ=37)
                                                     cAAATTCTGCCCCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCGTCCGCGCGCACGTTGACCCGCCCCCTAACCGGCGCCCGCAGGGGCGAggggg                                                                                        >  2:7841346/1‑139 (MQ=25)
                                                     cAAATACTGCACCGACGCGACGGACGAAAATGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGTGCCCCACCCGAAGCCGCTCCCTCGAggggggggggg                                                                                        >  1:1943611/1‑139 (MQ=25)
                                                      aaaTACTGCACCGACGCGACGGACTAAAAATTATTCGAAAGGTTGGTTTTCTCCCTCTCCCTGTGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGAc                                                                                       <  1:10448359/140‑1 (MQ=255)
                                                          aCTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCTCACCCTAAACCTCTCCCTCAAGGGAGAGGGGACCGAt                                                                                   >  2:1741047/1‑140 (MQ=255)
                                                           cTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCACAACCAAACCCAATCCCACAAAGGCCAGGGGAcccaac                                                                                  >  2:1172465/1‑136 (MQ=25)
                                                              cACCGACGCGACGGACGAAATAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGCCCCCCACCCGAAACCCCGCCCCCCCGGGAGAGGGGGCCgcgcgtg                                                                               >  1:6740453/1‑134 (MQ=21)
                                                              cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCCCCCCCAAACCCACACCCACCCGGGCGAGGGGACCGATCGAg                                                                               >  2:1926380/1‑140 (MQ=25)
                                                              cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTGTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCCCGTGCACCCGCCCCCAAACCCTCTCCCGCGGGGGGGGGGGGCCCGAGCggg                                                                               >  2:7456699/1‑138 (MQ=25)
                                                                   acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTTGACCCACACCCAAACCCACACCCACAAGGGAGAGGGGGCCGATAGAGcccca                                                                          >  2:814416/1‑136 (MQ=25)
                                                                   acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCCCGTGCACCCGCACCCGAAACCACTCCCACAAGGGCGAGGGGGCCCATCGAGCACaa                                                                          >  2:1536168/1‑140 (MQ=25)
                                                                   acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCTCACCCCAAACCAAAACCACAAGGGAGTGGGGTCCCATAGAGcaaac                                                                          >  2:984153/1‑137 (MQ=21)
                                                                   acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCACCCCCCAACACAACCCCCCCAGGGCGGGGGGGCCGCGCGAGccaaa                                                                          >  1:11312124/1‑136 (MQ=18)
                                                                   acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCACAACCAAAACCACAACCCAACAGGGGAGGTGACCGGACGAGcacca                                                                          >  1:6871546/1‑138 (MQ=18)
                                                                           gacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCACCCAAACCCACACCCACCAGGGGGGGGGGGCCGAGCGAGCCCACATATTgtg                                                                  >  1:11136253/1‑138 (MQ=21)
                                                                             ctaaaatGTATTCGTAAGGTTGGTTTTCCCCCACTCCCTGTGGGTGCGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagag                                                                <  1:9288578/138‑1 (MQ=38)
                                                                             cgACAAAGTATTCGAACGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagag                                                                <  2:11003528/140‑1 (MQ=255)
                                                                             cgAAAATTTTTTCGTAAGTTGGGTTTTCTCCCTCTCCCGGTGGTAGCGGGCCGGTGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagag                                                                <  1:1857641/140‑1 (MQ=38)
                                                                             cgAAAATGTATTCGTCAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCACTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagag                                                                <  2:1248625/140‑1 (MQ=255)
                                                                                  caGTATTCGTAAGGTTGGTTGTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtc                                                           <  2:1797093/139‑1 (MQ=255)
                                                                                  atgTATCCGTACGGTTGTTTTTCTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtc                                                           <  1:10058590/138‑1 (MQ=38)
                                                                                        actgacggttggttGTCTCCCGCTCCCTGTGGGAGAGGGCCGGGGCGCTGGCAGCAGCGCGCCCGTTCCCCCTCAACCTAACCCTCGCCCTCAAGGGAGAGGGGACCGCTCGAGCACAAAATTTGAGACATAtcttcttc                                                     <  2:8066520/134‑1 (MQ=255)
                                                                                          gTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTg                                                   <  2:3252305/140‑1 (MQ=255)
                                                                                          gTAGGGTTGGTTTTCCCCCTCGCCCTGGGGGTGTGGGCGGGGGTGTGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTg                                                   <  1:5015190/140‑1 (MQ=38)
                                                                                             tggttggttTTCTCCCTCCCCCTGTGGGAGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCt                                                <  1:1779575/139‑1 (MQ=255)
                                                                                                           cctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGTGAGAGGGTACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAAc                                  <  2:10008600/140‑1 (MQ=255)
                                                                                                           ccactccctGTGGGAGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAAc                                  <  2:5499802/137‑1 (MQ=255)
                                                                                                             tctccctGTGGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAg                                <  2:3624820/140‑1 (MQ=255)
                                                                                                              ctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCTCCCCCCAACCCTCTCCCGCAAGGGCGAGGGGACCGCTCGAGCCCCAAATTTGTGAGATATCTTAATATGTCTTTGAACAGAAGAACAGa                               >  2:10039254/1‑140 (MQ=25)
                                                                                                                         agagGGCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCACACCCCAACCCTCTCCCACAAAGGAGGGGGGACCGCACGAGCACCAATTTTTAGAGATATAATATTATGTGTTGTTAAAGAAGAAAAACAAAACGGGCtt                    >  2:8917424/1‑140 (MQ=255)
                                                                                                                          gagGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGACTTGTAACAGAAGAACAGAAAAGCGGGCttt                   <  2:11099628/140‑1 (MQ=255)
                                                                                                                           agGGCCGGGGTGAGGGCATCCGCGCGCACGTGCACCCTCACCCTAAACCTCACCCGCAAGGGGGAGGGGACCGATAGATCACCAATTTTGAGCGATATCTTAATCTGTCTTTTACCAGTAGAACCGAACAACCGGCgttt                  >  2:7285876/1‑140 (MQ=255)
                                                                                                                           agGGCCGGGGTGAGGGCATCAGCGCGCACGTTGAACCTCACCCAAAAACACAACCTCAAGGGTGATGGGGCCCAACGAGCACAAAATTTGATAGATATAGTATTATGTCATTTAAAAAAAAAACAAAAAATAGGGCttat                  >  2:3204320/1‑138 (MQ=255)
                                                                                                                            gggCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTg                 <  1:1370569/140‑1 (MQ=255)
                                                                                                                            gggCCGGGGTGCGGGCCTCCGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGCGATTTATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTg                 <  1:5618075/140‑1 (MQ=39)
                                                                                                                            gggCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAGGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTg                 <  1:5451307/140‑1 (MQ=255)
                                                                                                                             ggCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCACACCCAAAACCTCTACCAGACGGGTGTGGGGACCGCGAGAGCACAAATTTTGAGAGATATGATATTGTGTATTGTTAAAGAACTAAAGAAAATAgggggttttg                >  2:2608610/1‑133 (MQ=255)
                                                                                                                               ccGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCACCCAATCCCACTCCCTACAGGGTGAGGGGACCGATGCAGCACAAAATTTTTGAGATTTCTTATTGTGTCTTGTAACCGTCGAACAGAAAAGCGGGCTGTTgcgc              >  2:8374864/1‑140 (MQ=255)
                                                                                                                               ccGGGGTGAGGGCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGCTCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc              <  2:8477898/140‑1 (MQ=255)
                                                                                                                                       aGGGCATCAGCGCGCACGTTGACCCACCCCCAAACCCCCACCCACCAGGGGGAGGGGGCCGCTCGGGCCCAAAATTTTTGAGATATCTTATTCTGTTTTGTAACAGAAGAAAACAAAAAACGGCTGTTGGGCCGGAatat      >  2:485956/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                          
CAAAAAGAGATGAAAGGGCGTTATCCCAAA‑CATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCG  >  NZ_CP025268/164273‑164550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑