BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	581,201	T→C	pseudogene (667/667 nt)	CXP41_RS03085 ←	class I SAM‑dependent methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	581,201	0	T	C	99.7%	2288.7 / NA	659	pseudogene (667/667 nt)	CXP41_RS03085	class I SAM‑dependent methyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (372/284); minor base A (1/1); total (374/285)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 659 total aligned reads displayed.

ATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TT‑G‑ATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCTGATTG  >  NZ_CP025268/581069‑581336

           tGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGc                                                                                                                        >  1:7635604/1‑140 (MQ=255)

              tGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGa                                                                                                                     <  1:995172/140‑1 (MQ=255)

              tGAACCGTGTTGATACATCAACTGCACCTGAGAGTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTATTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGa                                                                                                                     <  2:335156/140‑1 (MQ=255)

               gatccGTGTGGATACATCAACTGCACCTGAAATTGAGTGGCCAACGAACCCGGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑AGGCTATTTTTTTCCTGAAGCGGaa                                                                                                                    <  2:6313707/137‑1 (MQ=255)

               gAACCGTGTTGATACATCAACTGCACCTNATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGaa                                                                                                                    <  1:5946788/140‑1 (MQ=255)

               gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGaa                                                                                                                    <  2:8397818/140‑1 (MQ=255)

               gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGaa                                                                                                                    <  1:4490387/140‑1 (MQ=255)

               gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCGGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGaa                                                                                                                    <  2:9117981/140‑1 (MQ=255)

                 aCCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGaaaa                                                                                                                  <  2:6913263/140‑1 (MQ=255)

                    gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                               >  1:684470/1‑140 (MQ=255)

                    gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                               <  1:2229856/140‑1 (MQ=255)

                    gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                               <  2:9487951/140‑1 (MQ=255)

                    gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                               <  1:4684667/140‑1 (MQ=255)

                    gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                               <  1:4220814/140‑1 (MQ=255)

                     tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                              >  1:7133844/1‑140 (MQ=255)

                     tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                              >  1:747760/1‑140 (MQ=255)

                     tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                              >  1:8135026/1‑140 (MQ=255)

                     tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                              >  1:4111473/1‑140 (MQ=255)

                     tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                              >  2:2280291/1‑140 (MQ=255)

                     tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                              >  1:10576136/1‑140 (MQ=255)

                     tgtTGATACATCAACTGCACATGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCATCACGTCTTAAGCAAGATCATGCTGAG‑GTTT‑G‑ATTATATTTTTTTCGTGAAGCGGTATACAtt                                                                                                              >  2:1413484/1‑140 (MQ=255)

                      gtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATta                                                                                                             <  1:1765179/140‑1 (MQ=255)

                           tACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTTATCATTGGGTTTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                        <  2:2771436/140‑1 (MQ=255)

                           tACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                        <  1:7397393/140‑1 (MQ=255)

                           tACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACAGGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                        <  1:10801538/140‑1 (MQ=255)

                            aCATCACCGGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGACTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑AGGCTATTGTTTTCCTTAAGCGGAAAACAGTACTACAg                                                                                                       <  2:8560043/140‑1 (MQ=255)

                             cATCAACTGCACCTGATCTTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATAATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTACCTGAAGCGGAAAACATAACTACAGt                                                                                                      >  2:3506636/1‑140 (MQ=255)

                             cATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGt                                                                                                      >  1:877269/1‑140 (MQ=255)

                               tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGCTTATGCCGCAGCACGTCTGAAGCAAGAACATGCGGCG‑G‑TTGG‑ATGCTCTTTTTTTCCTGATGCGGAAAACAGTACTACAGTAc                                                                                                    >  2:6200473/1‑140 (MQ=255)

                               tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                                    >  1:2765109/1‑140 (MQ=255)

                                     gCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGaa                                                                                              >  1:5730296/1‑140 (MQ=255)

                                        ccTGATATTGAGTGGCCTACGAACCCTGTCAGTGAGTAATCTTTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGATGCG‑G‑TT‑GTATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                           <  2:6043628/140‑1 (MQ=255)

                                        ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCCTTGGGAGTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                           >  2:11223149/1‑140 (MQ=255)

                                                 gAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGATGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                                  >  2:3218152/1‑140 (MQ=255)

                                                  aGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAAc                                                                                 >  2:198647/1‑140 (MQ=255)

                                                  aGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAAc                                                                                 >  1:11181607/1‑140 (MQ=255)

                                                  aGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAAc                                                                                 >  1:4839394/1‑140 (MQ=255)

                                                  aGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAAc                                                                                 >  1:3279828/1‑140 (MQ=255)

                                                     ggCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACAtt                                                                              >  2:1258677/1‑140 (MQ=255)

                                                      gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtc                                                                             >  2:10355852/1‑140 (MQ=255)

                                                       ccTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtct                                                                            >  2:408212/1‑140 (MQ=255)

                                                          aCGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGa                                                                         <  2:2997079/140‑1 (MQ=255)

                                                          aCGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGa                                                                         <  2:5005951/140‑1 (MQ=255)

                                                           cGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaa                                                                        >  1:3658769/1‑140 (MQ=255)

                                                            gcccccTGACAGGCAGTAATCAAGGGGCTTAAGCCGCAGCACGTCTAAAGCAAGACCATGCTGCGCA‑GG‑G‑ATGCTAAAATTTTCCTGAAGCGGAAAACATTACGACAGTACCTTGAACCTTGGTTTTAACATGCTCGaaa                                                                       <  2:7171817/137‑1 (MQ=255)

                                                            gAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAATCATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                       >  1:9499882/1‑140 (MQ=255)

                                                            gAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACCTTCTCGaaa                                                                       >  1:11261447/1‑140 (MQ=255)

                                                            gAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                       <  2:2520277/140‑1 (MQ=255)

                                                            gAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                       <  1:5277143/140‑1 (MQ=255)

                                                            gAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                       <  1:2488237/140‑1 (MQ=255)

                                                                ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                                   >  1:1316662/1‑140 (MQ=255)

                                                                ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                                   >  1:6314775/1‑140 (MQ=255)

                                                                ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                                   <  2:8823780/140‑1 (MQ=255)

                                                                 cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctc                                                                  >  1:11031046/1‑140 (MQ=255)

                                                                 cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctc                                                                  >  1:4968239/1‑140 (MQ=255)

                                                                 cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGAGGAAAACATTCCTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGatc                                                                  >  2:5736321/1‑140 (MQ=255)

                                                                  tGTCAGGTAGTAATCATTTGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATTCTATTTTTTTCCTGAAGCTGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCTAAATGctct                                                                 <  2:2417067/140‑1 (MQ=255)

                                                                  tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                                 <  1:6730764/140‑1 (MQ=255)

                                                                  tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                                 <  2:3694945/140‑1 (MQ=255)

                                                                  tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                                 <  1:3860519/140‑1 (MQ=255)

                                                                  agTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTAAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                                 <  2:9232731/139‑1 (MQ=255)

                                                                    tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                               <  2:5450915/140‑1 (MQ=255)

                                                                    tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                               >  1:10255687/1‑140 (MQ=255)

                                                                    tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                               <  1:6878798/140‑1 (MQ=255)

                                                                     cAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTgag                                                              <  2:4741510/140‑1 (MQ=255)

                                                                     cAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGAG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTgag                                                              <  2:10051988/140‑1 (MQ=255)

                                                                      aGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTgaga                                                             <  2:5278455/140‑1 (MQ=255)

                                                                          aGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACTTTCTCGAAATGCTCTGAGAGtat                                                         <  1:7866558/140‑1 (MQ=255)

                                                                          aGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtat                                                         <  1:9301095/140‑1 (MQ=255)

                                                                          aGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtat                                                         <  1:10664808/140‑1 (MQ=255)

                                                                          aGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtat                                                         >  2:1098909/1‑140 (MQ=255)

                                                                          aGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtat                                                         >  2:10567173/1‑140 (MQ=255)

                                                                           gTAATCATTGGGATTATGCCGCATCACGTCTTAAGCAAGAACATGCTGCG‑GTTT‑G‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtata                                                        <  1:8079596/140‑1 (MQ=255)

                                                                           gTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtata                                                        <  2:7635604/140‑1 (MQ=255)

                                                                            tAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTatat                                                       <  1:5070297/140‑1 (MQ=255)

                                                                            tAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTatat                                                       >  1:1980823/1‑140 (MQ=255)

                                                                              aTCATTGGGATTATGCCTCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTATATTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTAAACATTCTCGAAATGCTCTGAGAGTATAtgt                                                     >  2:3934670/1‑140 (MQ=255)

                                                                              aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATAtgt                                                     >  1:9680853/1‑140 (MQ=255)

                                                                                      gATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGcc                                             >  2:7355007/1‑140 (MQ=255)

                                                                                        ttATGCCGCAGCACGTCTTAACCAAGAACATGCTGCG‑G‑TTGG‑ATGCAACTTTTCTCCTGAAGCGGAAAACAATACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCctt                                           <  2:10791319/140‑1 (MQ=255)

                                                                                         tATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑T‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATAGGTGTTAAGCcttc                                          >  2:2147126/1‑140 (MQ=255)

                                                                                                cagcaCGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGaa                                   >  1:8978754/1‑140 (MQ=255)

                                                                                                cagcaCGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGaa                                   >  1:6503379/1‑140 (MQ=255)

                                                                                                cagcaCGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGaa                                   >  1:3453422/1‑140 (MQ=255)

                                                                                                     cGTCTTAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCttt                              <  1:1484636/140‑1 (MQ=255)

                                                                                                          tAAGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTgtgt                         >  1:2320156/1‑140 (MQ=255)

                                                                                                           aaGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTGCTTCGGAATCTTTTGTGtt                        >  1:8257384/1‑140 (MQ=255)

                                                                                                           aaGCAAGAACATGCTGCG‑G‑TTGG‑ATGCTATATTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCATGGTTTTAACATTCTAGAAAAGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGtt                        >  2:6548302/1‑140 (MQ=255)

                                                                                                                aGAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGaaa                   <  1:9094410/140‑1 (MQ=255)

                                                                                                                 gAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGaaaa                  >  1:3044697/1‑140 (MQ=255)

                                                                                                                 gAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGaaaa                  >  2:1891015/1‑140 (MQ=255)

                                                                                                                 gAACATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGaaaa                  >  2:1711527/1‑140 (MQ=255)

                                                                                                                 gAACATGCAGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTAAAAGAGTTCAGACTTCTTCGGAATCGTTAGTGATTGaaaa                  >  1:6070599/1‑140 (MQ=255)

                                                                                                                  aaCATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg                 >  1:8583754/1‑140 (MQ=255)

                                                                                                                  aaCATGCTGCG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg                 <  2:4323867/140‑1 (MQ=255)

                                                                                                                  aaCATGCTGCG‑A‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg                 >  1:10004766/1‑140 (MQ=255)

                                                                                                                          gcG‑G‑TTGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCttt         <  1:5450124/140‑1 (MQ=255)

                                                                                                                              ggttgg‑ATGCTATTCTTTTCCTGAAGCGGAAAACAATACTACAGTACCTTGAACCTGGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCt       <  2:4524769/135‑1 (MQ=255)

                                                                                                                                 tgg‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCTGAt    >  2:9760553/3‑140 (MQ=255)

                                                                                                                                  gg‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCTGAtt   >  2:8023788/2‑140 (MQ=255)

ATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑G‑TT‑G‑ATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCTGATTG  >  NZ_CP025268/581069‑581336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`