BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	861,872	A→G	373 (TAA→TAG)	ybiR →	anion transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	861,872	0	A	G	99.8%	2166.2 / NA	624	373 (TAA→TAG)	ybiR	anion transporter
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (379/244); minor base T (1/0); total (380/244)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 624 total aligned reads displayed.

CGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACC  >  NZ_CP025268/861736‑862008

   tGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGCTATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTg                                                                                                                                    >  1:7807112/1‑140 (MQ=255)

   tGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCACCTAGGGTCTg                                                                                                                                    >  1:9699839/1‑140 (MQ=255)

      cAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCa                                                                                                                                 <  1:5381895/140‑1 (MQ=255)

      cAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCa                                                                                                                                 <  2:3915648/140‑1 (MQ=255)

             gagtGCGCTACGTTTGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATaaaa                                                                                                                          <  2:10416747/137‑1 (MQ=255)

               ttGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGCTATGTTTTGTTAGTTATACTCCCGGCCACCTAGGGGCTGGCAAATaaaaag                                                                                                                        >  1:6095637/1‑139 (MQ=255)

               ttGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATaaaaaa                                                                                                                        >  2:2789738/1‑140 (MQ=255)

               ttGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAAAACCGATGGTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATaaaaaa                                                                                                                        >  1:1987137/1‑140 (MQ=255)

                tGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAg                                                                                                                       <  1:6978950/140‑1 (MQ=255)

                 gctcTACGTATGGCGAACGATCGCCGCTTCTGGTGGCGTTTCCATCTCTATTCAATACCTATGCTGTTGTGGGCGGCGTTGGTGGGAGATGTCTTGTTAGTTCTTCTCCCGGCCTACTAGGGTCTGGCAAATAAAAAAgg                                                                                                                      <  2:10998300/137‑1 (MQ=255)

                 gcgcTACGTATGGCGAACGTTCGCCGCCTGTGGTGGCGTTTCCTTCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATaaaaaatg                                                                                                                      <  2:7676990/140‑3 (MQ=255)

                 gcgcTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAgg                                                                                                                      <  2:4807013/140‑1 (MQ=255)

                 gcgcTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAgg                                                                                                                      <  1:7192220/140‑1 (MQ=255)

                 gcgcTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAgg                                                                                                                      <  2:2180427/140‑1 (MQ=255)

                 gcgcTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAgg                                                                                                                      <  2:6914224/140‑1 (MQ=255)

                   gcgaCGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGATATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCg                                                                                                                    <  2:9402926/137‑1 (MQ=255)

                   gcTACGTATGGCGCACGAGCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTGAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCg                                                                                                                    <  2:75698/140‑1 (MQ=255)

                   gcTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCg                                                                                                                    <  1:4065931/140‑1 (MQ=255)

                   gcTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCg                                                                                                                    <  2:1946179/140‑1 (MQ=255)

                   acTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCg                                                                                                                    <  2:10233337/139‑1 (MQ=255)

                    cTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCgg                                                                                                                   <  2:3579103/140‑1 (MQ=255)

                       cGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGGTGGTGGGATCTGTTTTGGTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGAtt                                                                                                                >  2:6272163/1‑140 (MQ=255)

                            ggCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAg                                                                                                           >  2:10037087/1‑140 (MQ=255)

                             gCGACCGAGCGCCGCCTCTGGGGGCGTTTCCATCTCTCTTCCATACCGAGGCTGTGGTGGGCGGCGTGGGTGGGATATGTTTTGTTAGTTAGCCGCCCGGCCAACTAGGGTCGGGCAAATAAAAAAGGCGGATTATGCGt                                                                                                          <  2:8700510/140‑1 (MQ=255)

                             gCGAACGATCGCCGCATCTGGTGGCGTGTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGt                                                                                                          <  1:7345882/140‑1 (MQ=255)

                              cGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCTTTTCCGATGCTGTTGTGGGCGGCGTGGGTGGGATATGTTTTGTTAGTTATACTGCCGGCCAACTAGGGTCTGGCAATTAAAAAAGGCGGATTATGAGTc                                                                                                         <  2:380206/140‑1 (MQ=255)

                                 aCGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGGTGTTGTGGGAGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGc                                                                                                      >  1:8129649/1‑140 (MQ=255)

                                 aCGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAgg                                                                                                                      <  2:539333/124‑1 (MQ=255)

                                 aCGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGc                                                                                                      >  1:3567591/1‑140 (MQ=255)

                                 aCGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGc                                                                                                      >  2:8361368/1‑140 (MQ=255)

                                 aCGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGGCTGGCAAATAAAAAAGGCGGATTATGAGTCCGc                                                                                                      >  1:5029483/1‑140 (MQ=255)

                                      cgccgcATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTCGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCtttt                                                                                                 >  2:107782/1‑140 (MQ=255)

                                            aTCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATTTATTGTTAGTAATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTa                                                                                           <  2:5033982/140‑1 (MQ=255)

                                                   ggCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTg                                                                                    <  1:4715046/140‑1 (MQ=255)

                                                     cGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGaa                                                                                  >  1:1570186/1‑140 (MQ=255)

                                                                ctATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAg                                                                       >  2:6033154/1‑140 (MQ=255)

                                                                   ttCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACg                                                                    >  1:6847506/1‑140 (MQ=255)

                                                                      aaTACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAAc                                                                 >  2:1676944/1‑140 (MQ=255)

                                                                        tACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCg                                                               >  2:1265204/1‑140 (MQ=255)

                                                                        tACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTGTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCg                                                               >  1:11142320/1‑140 (MQ=255)

                                                                           cGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCa                                                            >  1:3694270/1‑140 (MQ=255)

                                                                           cGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCa                                                            >  1:549543/1‑140 (MQ=255)

                                                                           cGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCa                                                            >  1:9271459/1‑140 (MQ=255)

                                                                           cGATGCTGGTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCa                                                            >  1:1433687/1‑140 (MQ=255)

                                                                           cGATGCTGGTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCa                                                            >  2:7401887/1‑140 (MQ=255)

                                                                             aTGCTGTTGTGTGCGGCGTTGGTGGGATATGTTTTTTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCTCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATc                                                          >  1:5665135/1‑140 (MQ=255)

                                                                             aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGACGATTAATTCAGACGAACCGGCATc                                                          >  1:7982928/1‑140 (MQ=255)

                                                                             aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATc                                                          >  1:3283364/1‑140 (MQ=255)

                                                                             aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATc                                                          >  1:8702008/1‑140 (MQ=255)

                                                                             aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATc                                                          >  1:8489702/1‑140 (MQ=255)

                                                                             aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATc                                                          >  1:495146/1‑140 (MQ=255)

                                                                                  gttgtGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACT‑CCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCTCGGAg                                                    <  2:10148259/140‑1 (MQ=255)

                                                                                    tgtgGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGc                                                   >  2:7145490/1‑140 (MQ=255)

                                                                                        ggcggcGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGtt                                               >  1:2857633/1‑140 (MQ=255)

                                                                                        ggcggcGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGtt                                               >  1:8360161/1‑140 (MQ=255)

                                                                                        ggcggcGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGtt                                               >  1:4267952/1‑140 (MQ=255)

                                                                                             cgTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTaa                                          >  1:10437449/1‑140 (MQ=255)

                                                                                             cgTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTaa                                          >  2:4875545/1‑140 (MQ=255)

                                                                                             cgTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTaa                                          >  1:337486/1‑140 (MQ=255)

                                                                                             cgTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTaa                                          >  1:3087267/1‑140 (MQ=255)

                                                                                             cgTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTaa                                          >  1:11308384/1‑140 (MQ=255)

                                                                                                  gtgtGATATGATTTGTTCGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTAGGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATGCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCt                                     <  2:11140898/140‑1 (MQ=255)

                                                                                                  gtggGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAATGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCt                                     <  2:4614408/140‑1 (MQ=255)

                                                                                                  gtggGATAGGTTTTGTTAGGTATCCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGCTTATGAGTCCGCCTTTTGCTTTACGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCt                                     <  1:11034156/140‑1 (MQ=255)

                                                                                                     ggATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTtca                                  <  1:5839053/140‑1 (MQ=255)

                                                                                                      gATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTtcat                                 <  2:4102550/140‑1 (MQ=255)

                                                                                                       atatGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTtcatc                                >  2:10583468/1‑140 (MQ=255)

                                                                                                       atatGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTtcatc                                >  1:10794454/1‑140 (MQ=255)

                                                                                                                gttagttaTACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTATTCGCTTCATCTAGaacaac                       >  2:9818830/1‑140 (MQ=255)

                                                                                                                gttagttaTACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGaacaac                       <  2:3329031/140‑1 (MQ=255)

                                                                                                                gttagttaTACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGaacaac                       <  2:8129649/140‑1 (MQ=255)

                                                                                                                gttagttaTACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGaacaac                       <  1:8971676/140‑1 (MQ=255)

                                                                                                                 ttagttaTACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAAcc                      <  2:2559267/140‑1 (MQ=255)

                                                                                                                 ttagttaTACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAAcc                      >  2:4336378/1‑140 (MQ=255)

                                                                                                                 ttagttaTACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAAACGGCATCCCGGAGCGGTTTTTAAGCGCTTCATCAAGAACAAcc                      >  2:2925575/1‑140 (MQ=255)

                                                                                                                    gttaTACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTgg                   >  2:11251591/1‑140 (MQ=255)

                                                                                                                    gttaTACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTgg                   >  1:1656675/1‑140 (MQ=255)

                                                                                                                     ttaTACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGt                  <  1:1346849/140‑1 (MQ=255)

                                                                                                                      tataCTCCCGGCCAACTAGGGTCTGGCAAATATAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTc                 >  2:6980334/1‑140 (MQ=255)

                                                                                                                      tataCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTc                 >  2:3165664/1‑140 (MQ=255)

                                                                                                                         aCTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAAc              <  1:9425105/140‑1 (MQ=255)

                                                                                                                               ggCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTgg        >  1:10708566/1‑140 (MQ=255)

                                                                                                                               ggCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTgg        >  2:9562078/1‑140 (MQ=255)

                                                                                                                               ggCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTgg        >  1:2266783/1‑140 (MQ=255)

                                                                                                                               ggCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTgg        >  1:2524340/1‑140 (MQ=255)

                                                                                                                               ggCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTgg        >  1:272193/1‑140 (MQ=255)

                                                                                                                               ggCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTgg        >  2:6635592/1‑140 (MQ=255)

                                                                                                                               ggCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCGTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTgg        >  1:2069184/1‑140 (MQ=255)

                                                                                                                                gCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGc       <  2:7770417/140‑1 (MQ=255)

                                                                                                                                gCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGc       <  2:4722661/140‑1 (MQ=255)

                                                                                                                                  cAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTg     <  2:9002316/140‑1 (MQ=255)

                                                                                                                                  cAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTg     <  1:6506167/140‑1 (MQ=255)

                                                                                                                                    aCTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAc   <  1:296470/140‑1 (MQ=255)

                                                                                                                                    aCTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAc   <  1:4452465/140‑1 (MQ=255)

                                                                                                                                    aCTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAc   <  2:5780396/140‑1 (MQ=255)

                                                                                                                                    aCTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAc   <  2:9183173/140‑1 (MQ=255)

                                                                                                                                    aCTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAc   <  1:10004338/140‑1 (MQ=255)

                                                                                                                                    aCTAGGGTCTGGCAAAGAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAc   <  1:6353675/140‑1 (MQ=255)

                                                                                                                                     cTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAcc  >  1:3833572/1‑140 (MQ=255)

                                                                                                                                     cTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAcc  >  1:5243457/1‑140 (MQ=255)

CGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGACC  >  NZ_CP025268/861736‑862008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`