BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	95,839	A→G	453 (TAA→TAG) I3V (ATT→GTT)	murF → mraY →	UDP‑N‑acetylmuramoyl‑tripeptide‑‑D‑alanyl‑D‑ alanine ligase phospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	95,839	0	A	G	99.9%	2548.6 / NA	731	453 (TAA→TAG) I3V (ATT→GTT)	murF mraY	UDP‑N‑acetylmuramoyl‑tripeptide‑‑D‑alanyl‑D‑ alanine ligase phospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (392/338); minor base C (0/1); total (392/339)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 731 total aligned reads displayed.

GATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATT  >  NZ_CP025268/95705‑95974

 aTAAAACTGCGTTAATTACGCGTCGTAAATTACTGATTGCTGAGCAACAGGTAATTACGATGTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTCGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTtggg                                                                                                                                   >  2:7015213/1‑139 (MQ=255)

  tAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTCATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTGtggct                                                                                                                                  >  1:34764/1‑140 (MQ=255)

           gTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACa                                                                                                                         >  1:9524638/1‑140 (MQ=255)

           gTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACAGGTAAGTTTGGCTGGCCGAACa                                                                                                                         >  2:6767712/1‑140 (MQ=255)

           gTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATGTTAGTTAAGGGTTCACGTCGTGCCGCCCTGGAAGAGGTAGTACGGGCTTTACAGGAGAATGGGACAGGTTAGGTTGGCTGGCCGatcg                                                                                                                         >  1:9949263/1‑137 (MQ=255)

           gTTAATTACGCGGCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACa                                                                                                                         >  2:1060161/1‑140 (MQ=255)

               aTTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTg                                                                                                                     <  2:3754208/140‑1 (MQ=255)

                  aCGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTc                                                                                                                  <  1:7941754/140‑1 (MQ=255)

                  aCGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTc                                                                                                                  <  1:5374820/140‑1 (MQ=255)

                    gcgTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGAGATGTTAGTTTGGCTGGCCGAACATTTGGTCaa                                                                                                                >  1:10116734/1‑140 (MQ=255)

                     cgTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCaaa                                                                                                               <  1:5894571/140‑1 (MQ=255)

                         ttAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAAtatt                                                                                                           >  2:3475106/1‑140 (MQ=255)

                            aaTTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGGGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATattatt                                                                                                        <  2:7692606/140‑1 (MQ=255)

                               tACTGCTTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCg                                                                                                     <  1:4653961/140‑1 (MQ=255)

                                    aTTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCTCGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCttt                                                                                                >  2:3867239/1‑140 (MQ=255)

                                    aTTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCAGGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCttt                                                                                                >  1:2950496/1‑140 (MQ=255)

                                     ttGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTa                                                                                               <  2:2804681/140‑1 (MQ=255)

                                     ttGCTGAGCAACAGGTAATTACGATTTGAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTGGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTa                                                                                               <  2:5625035/140‑1 (MQ=255)

                                     ccgcagAGCAACAGGTACTTACGATTGTAGAGAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGACTGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAACTATTATTCCGGCTTTa                                                                                               <  2:10839677/135‑1 (MQ=255)

                                     aagCTGCGCAACCGGTAAGGACGATTTTACTTAAGGGTTCTCGTAGGGCCGCCATGGAAGAGGTAGTACGCGCTTTAACGGAGACTGTGACCTGTTCGTTGGGCTTGCCTAACATTTGGTCAACTAGTCTTCCGGCTTTa                                                                                               <  2:8558138/138‑1 (MQ=255)

                                      tGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTaa                                                                                              >  1:10039188/1‑140 (MQ=255)

                                         tGAGCAACAGGTAATTACGATTGTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGACTGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGt                                                                                           <  2:5936721/140‑1 (MQ=255)

                                           aGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCGTTAACGTCt                                                                                         >  1:9227965/1‑140 (MQ=255)

                                               aCAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTc                                                                                     <  1:6273277/140‑1 (MQ=255)

                                                cAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTcc                                                                                    >  2:10260217/1‑140 (MQ=255)

                                                cAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTcc                                                                                    >  2:7168339/1‑140 (MQ=255)

                                                cAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTcc                                                                                    >  2:8357328/1‑140 (MQ=255)

                                                cAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTcc                                                                                    >  1:4379170/1‑140 (MQ=255)

                                                cAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTcc                                                                                    >  1:2716648/1‑140 (MQ=255)

                                                   gTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACAGGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTAt                                                                                 >  1:499180/1‑140 (MQ=255)

                                                     aaTTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATTGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACAtt                                                                                                                       <  1:10640178/100‑1 (MQ=255)

                                                     aaTTACGATTGTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGCTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGGTTGGCTGCCCGAACATTTGGTCAAATATTATTCAGGCTTTAACGTCTTTTGCTATCt                                                                               >  2:6551929/1‑140 (MQ=255)

                                                             ttttAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTc                                                                       >  1:3872335/1‑140 (MQ=255)

                                                             ttttAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTc                                                                       >  1:5580599/1‑140 (MQ=255)

                                                             ttttAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTc                                                                       >  1:8310508/1‑140 (MQ=255)

                                                               ttAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTcgc                                                                     >  1:8444950/1‑140 (MQ=255)

                                                                tAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTcgcg                                                                    <  2:6413398/140‑1 (MQ=255)

                                                                 aGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCgcgc                                                                   <  2:2599706/140‑1 (MQ=255)

                                                                 aGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCgcgc                                                                   <  2:7859972/140‑1 (MQ=255)

                                                                        gggtCACGTAGTGCCGCCATGGACGAGGGAGTACGCGCTTTACAGGAGACTGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTGTCCTATCTGACGTTTCGCGCCATCGTc                                                            <  2:7271207/137‑1 (MQ=255)

                                                                        ggTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTACCGTCTTTTCCTATCTGACGTTTCGCGCCATCGTc                                                            <  1:11286846/140‑1 (MQ=255)

                                                                        ggTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTc                                                            >  2:4183065/1‑140 (MQ=255)

                                                                        ggTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTc                                                            <  1:5753675/140‑1 (MQ=255)

                                                                        ggTTCACGTAGTGCCGCCATGGAAGAGGGAGTACGCGCTTTACAGGCGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGAGGTTTCGCGCCATCGTc                                                            >  2:9668189/1‑140 (MQ=255)

                                                                            cACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGcc                                                        >  1:8906292/1‑140 (MQ=255)

                                                                            cACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGcc                                                        >  1:7302660/1‑140 (MQ=255)

                                                                            cACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGcc                                                        >  2:93154/1‑140 (MQ=255)

                                                                             aCGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCTCCATCGTCAGCct                                                       <  1:9720468/140‑1 (MQ=255)

                                                                             aCGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCct                                                       <  2:7480221/140‑1 (MQ=255)

                                                                               gTAGTGCCGCCATGGAAGCGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAACTATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCctgc                                                     <  1:4169904/140‑1 (MQ=255)

                                                                                  gTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGa                                                  <  1:1359223/140‑1 (MQ=255)

                                                                                  gTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGGTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGa                                                  >  2:8175897/1‑140 (MQ=255)

                                                                                    gccgccATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGAcc                                                >  2:9429949/1‑140 (MQ=255)

                                                                                    gccgccATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGAcc                                                >  1:10473543/1‑140 (MQ=255)

                                                                                     ccgccATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACcg                                               <  1:6641678/140‑1 (MQ=255)

                                                                                     ccgccATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACcg                                               <  1:10929495/140‑1 (MQ=255)

                                                                                       gccATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACcgcg                                             <  2:10473543/140‑1 (MQ=255)

                                                                                       gccATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACcgcg                                             <  1:644156/140‑1 (MQ=255)

                                                                                       gccATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACcgcg                                             <  1:3791481/140‑1 (MQ=255)

                                                                                       gccATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACcgcg                                             <  1:4083808/140‑1 (MQ=255)

                                                                                             gAAGAGGTCGTACGCGCTTTACAGGAGAATGGGACTTGTTAGTTTGGCTGGCCGAACAGTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTACGCGCCATCGTCAGCCTGCTGACCGCGCTGTTc                                       >  2:8962159/1‑140 (MQ=255)

                                                                                             gAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTc                                       >  2:11085632/1‑140 (MQ=255)

                                                                                              aaGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCa                                      <  2:4915760/140‑1 (MQ=255)

                                                                                              aaGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCa                                      <  2:454882/140‑1 (MQ=255)

                                                                                                 agGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCAtct                                   <  1:1922451/140‑1 (MQ=255)

                                                                                                     agtaCGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCAtt                               >  2:2210152/1‑140 (MQ=255)

                                                                                                     agtaCGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCAtt                               <  1:8663038/140‑1 (MQ=255)

                                                                                                       taCGCGCTTTACAGGAGAATGGGACAGGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTAACTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCAACTCATtgt                             >  2:1790478/1‑140 (MQ=255)

                                                                                                        aCGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATtgtg                            >  1:6878448/1‑140 (MQ=255)

                                                                                                        aCGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATtgtg                            >  2:1132013/1‑140 (MQ=255)

                                                                                                           cgcTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGAt                         <  1:2080302/140‑1 (MQ=255)

                                                                                                            gcTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATg                        >  1:3611487/1‑140 (MQ=255)

                                                                                                            gcTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATg                        >  1:6080569/1‑140 (MQ=255)

                                                                                                             cTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACcg                                               >  2:5011542/1‑116 (MQ=255)

                                                                                                             cTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATgg                       >  2:2402803/1‑140 (MQ=255)

                                                                                                              attACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATATGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCAACTCATTGTGGATggg                      <  1:5320582/139‑1 (MQ=255)

                                                                                                               ttACAGGAGACTGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc                     <  2:1961395/140‑1 (MQ=255)

                                                                                                               ttACAGGAGAATGGTACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc                     <  2:8063971/140‑1 (MQ=255)

                                                                                                               ttACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc                     <  1:3321671/140‑1 (MQ=255)

                                                                                                               ttACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc                     <  1:3157299/140‑1 (MQ=255)

                                                                                                               ttACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc                     <  2:2975113/140‑1 (MQ=255)

                                                                                                               ttACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc                     <  1:5211566/140‑1 (MQ=255)

                                                                                                                tACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGcc                    >  1:10252319/1‑140 (MQ=255)

                                                                                                                    ggAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCcgcg                <  1:2401734/140‑1 (MQ=255)

                                                                                                                     gagaATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTAAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCTCCATCGTCAGCCTGCTGAACGCGCTGTTCATCTCATTGTGGATGGGCCCGCGt               <  1:2224799/140‑1 (MQ=255)

                                                                                                                        aaTGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATg            >  2:10927504/1‑140 (MQ=255)

                                                                                                                        aaTGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATg            >  2:8843474/1‑140 (MQ=255)

                                                                                                                        aaTGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATg            >  1:1180117/1‑140 (MQ=255)

                                                                                                                        aaTGGGACAGGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCGGTTCATCTCATTGTGGATGGGCCCGCGTCTg            >  2:1232225/1‑140 (MQ=255)

                                                                                                                          tGGGCCATGTTAGTTTGGCTGGCCGAACAGTTGGTCCACTAGTATTCCGGCTGTAACGTCTTTTCCTATCTGACGTTTCGCGCCAGCGTCAGCCTGCTGACCGCGCTGTTCATAGCATTGTGGATGGGCCCGCGTATGAt          >  2:4752481/1‑140 (MQ=255)

                                                                                                                          tGGGACATGTTCGTTTGGCTGGCCGAACATTTGGTGAAATATTATTCCGGCTTTAACTTCTATTCCTATCTGACGGTTCGCTCACTCGTCTGCCTGCTGCCGGAGCTGGTCATGTGATTGTGGATGGGCGGGCGTATGAt          >  2:3298703/1‑140 (MQ=255)

                                                                                                                          tGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCctgct                                                    >  1:1654678/1‑98 (MQ=255)

                                                                                                                          tGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGAt          >  2:533712/1‑140 (MQ=255)

                                                                                                                          tGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGAt          >  2:1559169/1‑140 (MQ=255)

                                                                                                                          tGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGAt          >  2:8721419/1‑140 (MQ=255)

                                                                                                                          tGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGAt          >  1:2624187/1‑140 (MQ=255)

                                                                                                                          tGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGAt          >  1:7627865/1‑140 (MQ=255)

                                                                                                                               cATGTTAGTTTGGCTGGCCGAACAGTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGAGGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCGGTGCTTCTCATGGGGGATGGGCCCGCGGAGGATTGCtg     >  2:6166136/1‑139 (MQ=255)

                                                                                                                                  gTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCAtt  >  1:11118142/1‑140 (MQ=255)

                                                                                                                                  gTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTAGGATTGCTCAtt  >  1:7453090/1‑140 (MQ=255)

GATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATT  >  NZ_CP025268/95705‑95974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`