BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,423,978	A→G	163 (TAA→TAG) S51S (AGT→AGC)	sieB → ydaF ←	superinfection exclusion B family protein YdaF family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,423,978	0	A	G	99.8%	2062.0 / NA	597	163 (TAA→TAG) S51S (AGT→AGC)	sieB ydaF	superinfection exclusion B family protein YdaF family protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (302/294); minor base C (1/0); total (303/294)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 597 total aligned reads displayed.

ATGAAAAAATTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGA  >  NZ_CP025268/1423841‑1424114

aTGAAAAAATTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCt                                                                                                                                         <  1:4350221/140‑1 (MQ=255)

aTGAAAAAATTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCt                                                                                                                                         <  2:2391052/140‑1 (MQ=255)

aTGAAAAAATTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCt                                                                                                                                         <  2:8961350/140‑1 (MQ=255)

  gAAAAAATTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTa                                                                                                                                       <  1:10242929/140‑1 (MQ=255)

  gAAAAAATTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTa                                                                                                                                       <  1:10938494/140‑1 (MQ=255)

  gAAAAAATTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTa                                                                                                                                       <  1:244186/140‑1 (MQ=255)

  gAAAAAATTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTa                                                                                                                                       <  1:7314568/140‑1 (MQ=255)

    aaaaaTTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACt                                                                                                                                     <  2:10514418/140‑1 (MQ=255)

    aaaaaTTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACt                                                                                                                                     <  2:4359722/140‑1 (MQ=255)

    aaaaaTTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACt                                                                                                                                     <  1:225571/140‑1 (MQ=255)

    aaaaaTTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACt                                                                                                                                     <  1:7588700/140‑1 (MQ=255)

    aaaaaTTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACt                                                                                                                                     <  2:10916413/140‑1 (MQ=255)

      aaaTTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACtgt                                                                                                                                   <  1:1759549/140‑1 (MQ=255)

      aaaTTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACtgt                                                                                                                                   <  2:7174106/140‑1 (MQ=255)

        aTTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACtgtgt                                                                                                                                 >  2:7053544/1‑140 (MQ=255)

         ttAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAACATCCTCCCATTTTAGGAAGATATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACtgtgtg                                                                                                                                <  2:1602726/140‑1 (MQ=255)

         ttAGAACGGAAGGGCAACATTCAACATCCGAGTGATAGCGCAAACTGTTCTTATTATCTCGACACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACtgtgtg                                                                                                                                <  2:6776801/140‑1 (MQ=255)

          tAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGc                                                                                                                               <  1:2729404/140‑1 (MQ=255)

                ggAAGGGCATCATTCAACTTCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAAGACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTcc                                                                                                                         <  2:10750827/140‑1 (MQ=255)

                        atcatTCAACCTCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTTACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCg                                                                                                                 <  2:7613842/140‑1 (MQ=255)

                               aaCATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCa                                                                                                          >  2:11076984/1‑140 (MQ=255)

                                aCATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCAc                                                                                                         <  2:9623791/140‑1 (MQ=255)

                                    cAGAGTGATCGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAgg                                                                                                     >  1:10116389/1‑139 (MQ=255)

                                    cAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGt                                                                                                     >  2:3056372/1‑140 (MQ=255)

                                    cAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTGATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCTTCGGCGCGCAGCAGt                                                                                                     >  2:9810504/1‑140 (MQ=255)

                                       agTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTc                                                                                                  >  2:2145466/1‑140 (MQ=255)

                                       agTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTc                                                                                                  >  2:10284807/1‑140 (MQ=255)

                                       agTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTc                                                                                                  >  1:11226908/1‑140 (MQ=255)

                                        gTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCg                                                                                                 <  1:6968853/140‑1 (MQ=255)

                                           aTAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑tt                                                                                             >  2:5707351/1‑140 (MQ=255)

                                           aTAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑tt                                                                                             >  1:1991734/1‑140 (MQ=255)

                                            tAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑ttt                                                                                            >  1:5010733/1‑140 (MQ=255)

                                             aGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑tttt                                                                                           >  2:4725464/1‑140 (MQ=255)

                                              gcgcAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTa                                                                                          >  2:2959853/1‑140 (MQ=255)

                                              gcgcAAACTGGTCTTTTTATCTCGTCACCGATATATTCTCCCAGTTTATGTAGTTATTCTGGAACTGCAGGAGTGGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGGGGGCAGCAGTTTCGG‑ttttg                                                                                          >  2:1827747/1‑139 (MQ=255)

                                               cgcAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTaa                                                                                         >  2:3341327/1‑140 (MQ=255)

                                               cgcAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTaa                                                                                         <  1:3245424/140‑1 (MQ=255)

                                               cgcAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTaa                                                                                         >  1:110667/1‑140 (MQ=255)

                                               cgcAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTaa                                                                                         >  1:3215627/1‑140 (MQ=255)

                                                 cAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TGTTAAAt                                                                                       >  1:10633926/1‑140 (MQ=255)

                                                   aaCTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGt                                                                                     <  2:8203740/140‑1 (MQ=255)

                                                    aCTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGtt                                                                                    <  2:3170317/140‑1 (MQ=255)

                                                      tGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGtttt                                                                                  <  1:5776809/140‑1 (MQ=255)

                                                        ttCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTTAAATGTTTTg                                                                                 >  2:8295258/1‑140 (MQ=255)

                                                         tCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCt                                                                               <  1:300317/140‑1 (MQ=255)

                                                          cTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCtt                                                                              <  2:146754/140‑1 (MQ=255)

                                                          cTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCtt                                                                              <  2:9885128/140‑1 (MQ=255)

                                                          cTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCtt                                                                              <  1:608094/140‑1 (MQ=255)

                                                           ttattaTCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCttt                                                                             <  2:2639860/140‑1 (MQ=255)

                                                                atCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGta                                                                        <  1:9667454/140‑1 (MQ=255)

                                                                atCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGta                                                                        <  2:8546752/140‑1 (MQ=255)

                                                                atCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGta                                                                        <  1:4725464/140‑1 (MQ=255)

                                                                  ctcGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGtata                                                                      >  1:10000786/1‑140 (MQ=255)

                                                                  ctcGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGtata                                                                      >  1:3826455/1‑140 (MQ=255)

                                                                     gTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGt                                                                   >  1:8912444/1‑140 (MQ=255)

                                                                     gTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGt                                                                   >  1:6493297/1‑140 (MQ=255)

                                                                       cACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCa                                                                 <  2:4492533/140‑1 (MQ=255)

                                                                       cACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCa                                                                 >  2:6393693/1‑140 (MQ=255)

                                                                       cACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCa                                                                 >  1:4579932/1‑140 (MQ=255)

                                                                       cACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCa                                                                 <  1:7173950/140‑1 (MQ=255)

                                                                       cACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCa                                                                 >  1:8324266/1‑140 (MQ=255)

                                                                       cACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCAAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCa                                                                 >  2:5568121/1‑140 (MQ=255)

                                                                            aaaaaTACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTTATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGta                                                                        <  1:9281809/128‑1 (MQ=255)

                                                                            aaaaaTACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGta                                                                        >  2:9281809/1‑128 (MQ=255)

                                                                                 tACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGaa                                                       <  2:3614092/140‑1 (MQ=255)

                                                                                    tCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGt                                                    >  1:80152/1‑140 (MQ=255)

                                                                                      ccATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTc                                                  >  1:5953936/1‑140 (MQ=255)

                                                                                         ttttATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAATTGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCAt                                               <  2:2816020/140‑1 (MQ=255)

                                                                                              tGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTgg                                          <  1:10794639/140‑1 (MQ=255)

                                                                                              tGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTgg                                          <  1:983209/140‑1 (MQ=255)

                                                                                              tGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTgg                                          >  1:3366748/1‑140 (MQ=255)

                                                                                              tGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTGTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGTACGTTCCATCCTgg                                          >  1:7783182/1‑140 (MQ=255)

                                                                                                    tATTCTGGAACAGCTGGAGTAGTCGTGTTAATGGTTTGCTTACTGTGTGCTTCTCCAACGATCGGCGCGCCGCTGGTTCGG‑TGTTTATTGGTTTGCTTGTGGTATTCGTCATGGGTGTGAAGGTGGCATCCTGGTTgggg                                    >  2:6051975/1‑140 (MQ=255)

                                                                                                    tATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTgggg                                    >  1:5165998/1‑140 (MQ=255)

                                                                                                     aTTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGa                                   <  1:8152406/140‑1 (MQ=255)

                                                                                                      ttCTTGAACATCAGGCGTAGACGCTTTAATCGTTCGCTAACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGaa                                  <  1:632571/140‑1 (MQ=37)

                                                                                                        cTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAaca                                <  1:8561818/140‑1 (MQ=255)

                                                                                                             aCAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACAcgccg                           <  2:4628674/140‑1 (MQ=255)

                                                                                                             aCAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACAcgccg                           <  1:1367006/140‑1 (MQ=255)

                                                                                                                gcagGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGcac                        >  1:3639376/1‑140 (MQ=255)

                                                                                                                gcagGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGcac                        <  2:5147635/140‑1 (MQ=255)

                                                                                                                gcagGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGcac                        >  1:5560105/1‑140 (MQ=255)

                                                                                                                gcagGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGcac                        >  1:1512890/1‑140 (MQ=255)

                                                                                                                 cagGAGTAGAGGTTTTAATCGATAGCTTACTGTGTGCTTCTCCAAGCATCGGCGCGCAGCAGTTTCGG‑TTTTAAATGGGTTGCGGTTGGTTTAGGTCATGGCCGGGAACGTTCCATCCTGGGTGGGGAACAcgccggccg                       >  2:6261580/1‑136 (MQ=255)

                                                                                                                 cagGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGcaca                       >  2:4229258/1‑140 (MQ=255)

                                                                                                                  cggAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCCCCAGTTTCGG‑TTTTAAATGTTGTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCacac                      <  2:8829185/139‑1 (MQ=255)

                                                                                                                     aGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAg                   >  1:6625207/1‑140 (MQ=255)

                                                                                                                      gTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAgg                  >  2:1256304/1‑140 (MQ=255)

                                                                                                                      gTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGGTGGGGAACACGGCGCGCACCAgg                  >  1:6816934/1‑140 (MQ=255)

                                                                                                                       tAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAggg                 >  1:2096775/1‑140 (MQ=255)

                                                                                                                         gACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGAt               >  2:489766/1‑140 (MQ=255)

                                                                                                                         gACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATACTGGTTGTTGAACACGCCGCACACCAGGGAt               >  2:7979991/1‑140 (MQ=255)

                                                                                                                          aCGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGAtt              <  1:5690942/140‑1 (MQ=255)

                                                                                                                           cGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGGTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTc             >  2:7855503/1‑140 (MQ=255)

                                                                                                                              tttAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCgtt          >  2:7301042/1‑140 (MQ=255)

                                                                                                                              tttAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCgtt          >  1:3910470/1‑140 (MQ=255)

                                                                                                                              tttAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCgtt          >  2:3939623/1‑140 (MQ=255)

                                                                                                                               ttAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCgttg         >  1:4230308/1‑140 (MQ=255)

                                                                                                                                 aaTCGTTAGCGTACTGAGTGCTTCTCCAACCATCGGCGCGCACCAGTGTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCgttttt       >  2:5875962/1‑140 (MQ=25)

                                                                                                                                      ttAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGa  <  2:1969319/140‑1 (MQ=255)

ATGAAAAAATTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGG‑TTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGA  >  NZ_CP025268/1423841‑1424114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`