BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,469,308	A→G	317 (TAA→TAG) N7D (AAC→GAC)	paaX → paaY →	phenylacetic acid degradation operon negative regulatory protein PaaX phenylacetic acid degradation protein PaaY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,469,308	0	A	G	99.8%	1937.1 / NA	556	317 (TAA→TAG) N7D (AAC→GAC)	paaX paaY	phenylacetic acid degradation operon negative regulatory protein PaaX phenylacetic acid degradation protein PaaY
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (209/346); minor base T (1/0); total (210/346)
Fisher's exact test for biased strand distribution p-value = 3.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 556 total aligned reads displayed.

GCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGG  >  NZ_CP025268/1469171‑1469443

gcgcCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGAc                                                                                                                                       <  2:7074697/140‑1 (MQ=255)

   ccTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGt                                                                                                                                    <  1:8892967/140‑1 (MQ=255)

   ccTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGt                                                                                                                                    >  2:7430666/1‑140 (MQ=255)

   ccTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGt                                                                                                                                    <  1:2045156/140‑1 (MQ=255)

   ccTGCTGCTTTAGCGGTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGt                                                                                                                                    <  2:6012111/140‑1 (MQ=255)

    ctgctgCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTc                                                                                                                                   >  1:307400/1‑140 (MQ=255)

     tgctgcTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCt                                                                                                                                  >  2:4442108/1‑140 (MQ=255)

      gctgctTTAGCGTTCGTTAGTGAAACAGGGGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATGGAACAGGCGGCGTTATGCCAATTTATCAGATAGACGGTCTg                                                                                                                                 >  2:3772761/1‑140 (MQ=255)

         gctTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACt                                                                                                                              <  1:4928086/140‑1 (MQ=255)

             tAGCGTTCGTTAGTGAACAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCgg                                                                                                                          <  1:3898341/140‑1 (MQ=255)

              aGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTTTATTTTCCACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGt                                                                                                                         <  2:854330/140‑1 (MQ=255)

              aGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGt                                                                                                                         <  1:1115931/140‑1 (MQ=255)

              aGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGATTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGt                                                                                                                         <  1:1176295/140‑1 (MQ=255)

                  ttcgttAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTtgtg                                                                                                                     >  1:2990467/1‑140 (MQ=255)

                    cgttAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCCACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGcc                                                                                                                   >  2:10623810/1‑140 (MQ=255)

                    cgttAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTGGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGcc                                                                                                                   >  1:69322/1‑140 (MQ=255)

                    cgttAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTAGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGcc                                                                                                                   >  1:9843458/1‑140 (MQ=255)

                    cgatAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGcc                                                                                                                   >  2:10523002/4‑140 (MQ=255)

                       tAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCaga                                                                                                                <  2:8775028/140‑1 (MQ=255)

                       gaGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCaga                                                                                                                <  1:7397402/139‑1 (MQ=255)

                          tGAAAAAGGTGAAACCTGGGTCGGTGAACTGCCTTCGCCGGGAAGCCTGTATTTTCAACCTGTTGGCGGCTAGAATATTGAACAGGAGTCGTTATGCCAATTTAACAGATAGACGGTCTGACTCCGGTTGTGCCagaaga                                                                                                             <  2:11285263/140‑1 (MQ=255)

                                   tGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGt                                                                                                    <  2:2764830/140‑1 (MQ=255)

                                    gAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTc                                                                                                   >  2:3034066/1‑140 (MQ=255)

                                    gAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCGGACTCCGGTTGTGCCAGAAGCGAGTTTTGTc                                                                                                   <  2:9738195/140‑1 (MQ=255)

                                         cTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATcc                                                                                              <  2:4782612/140‑1 (MQ=255)

                                          tcggtcggTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCg                                                                                             <  2:7783622/140‑1 (MQ=255)

                                           cggtcggtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATCTTGAACAGGAGGCGTTATGCCAAGTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGa                                                                                            >  1:8210623/1‑140 (MQ=255)

                                           cggtcggtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGa                                                                                            <  2:4031232/140‑1 (MQ=255)

                                           cggtcggtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGa                                                                                            <  2:2100633/140‑1 (MQ=255)

                                              tcggtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACCGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAg                                                                                         <  2:833880/140‑1 (MQ=255)

                                              tcggtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAg                                                                                         <  1:920160/140‑1 (MQ=255)

                                              tcggtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAg                                                                                         <  1:2756796/140‑1 (MQ=255)

                                              tcggtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAg                                                                                         <  2:6639919/140‑1 (MQ=255)

                                              tcggtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAg                                                                                         <  2:926078/140‑1 (MQ=255)

                                                 gtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCgg                                                                                      >  2:2975881/1‑140 (MQ=255)

                                                 gtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCgg                                                                                      >  2:7943140/1‑140 (MQ=255)

                                                  tGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGt                                                                                     >  1:1665021/1‑140 (MQ=255)

                                                        gcctgcGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGAt                                                                               >  1:10711053/1‑140 (MQ=255)

                                                        gcctgcGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGAt                                                                               <  2:5507167/140‑1 (MQ=255)

                                                        gcctgcGCCGGGAAGCCGGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCAGACTCCGGATGAGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGag                                                                               >  1:9581455/1‑139 (MQ=255)

                                                         cctgcGCCGGGTAGCCTGTATTTTCAACGTTTTGGCGGCTTGTATATTGAACAGGAGGCGTTATGCCAATTTATCTGATTGACGGTCTGACTCCGGTTGGGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATc                                                                              <  2:5067426/140‑1 (MQ=255)

                                                           tgcGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCgg                                                                            <  1:7638271/140‑1 (MQ=255)

                                                           tgcGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCgg                                                                            <  2:1344454/140‑1 (MQ=255)

                                                           tgcGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCgg                                                                            <  1:931772/140‑1 (MQ=255)

                                                           tgcGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCgg                                                                            <  1:4149709/140‑1 (MQ=255)

                                                            gcgcCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGc                                                                           >  2:7375635/1‑140 (MQ=255)

                                                            gcgcCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGc                                                                           >  2:4549755/1‑140 (MQ=255)

                                                             cgcCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGACAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCg                                                                          <  2:10045547/140‑1 (MQ=255)

                                                              gcCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGa                                                                         >  1:8533284/1‑140 (MQ=255)

                                                                cGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATg                                                                       <  1:3618951/140‑1 (MQ=255)

                                                                cGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATg                                                                       <  1:673518/140‑1 (MQ=255)

                                                                cGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATg                                                                       <  2:4269330/140‑1 (MQ=255)

                                                                cGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATg                                                                       >  2:1886294/1‑140 (MQ=255)

                                                                   gAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTa                                                                    >  1:1853486/1‑140 (MQ=255)

                                                                   gAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTa                                                                    >  1:7076041/1‑140 (MQ=255)

                                                                       ccTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATtct                                                                >  1:4507086/1‑140 (MQ=255)

                                                                          gTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCgg                                                             >  1:3242759/1‑140 (MQ=255)

                                                                            cttttCAACGGTTTGGCGGCGTGAATATTGACCAGGAGGCGTGATGCCACGTTATCAGCTCGCCGGTCTGACTCCGGTTGTGCCAGACGAGAGTTTTGTCAATCGGACCGCGGTTTTGATCGGCGATGGAATTCTCGGCa                                                           >  2:7227370/2‑140 (MQ=255)

                                                                            aTTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCa                                                           <  2:3615845/140‑1 (MQ=255)

                                                                             tgttCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGACGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCaa                                                          <  2:5650545/138‑1 (MQ=255)

                                                                              tttCAACGTTTTGGCGGCTTGAATATTGACCAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAg                                                         <  2:9474081/140‑1 (MQ=255)

                                                                              tttCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAg                                                         <  2:3541327/140‑1 (MQ=255)

                                                                              tttCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAg                                                         <  2:10131163/140‑1 (MQ=255)

                                                                              tttCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAg                                                         <  1:5444471/140‑1 (MQ=255)

                                                                                 cAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGc                                                      >  1:5556718/1‑140 (MQ=255)

                                                                                 cAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTACTGATCGGCGATGTTATTCTCGGCAAGGGc                                                      >  2:2530546/1‑140 (MQ=255)

                                                                                  aaCGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGACGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCCAGGGCg                                                     >  1:10379369/1‑140 (MQ=255)

                                                                                       tttGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTACATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTAc                                                >  1:8424228/1‑140 (MQ=255)

                                                                                         gggcggcTTGAATATTGAACAGGAGGCGTTATGCCAATGTCTCAGAGAGACGGTCTGACTCCGGTTGAGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGt                                              <  2:8119373/139‑1 (MQ=255)

                                                                                               ctagAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGcc                                        <  2:1427708/137‑1 (MQ=255)

                                                                                               cTTGATTTTTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTGGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGcc                                        <  2:7650834/140‑1 (MQ=255)

                                                                                               cTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGcc                                        >  2:6289892/1‑140 (MQ=255)

                                                                                               cTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGcc                                        <  2:111426/140‑1 (MQ=255)

                                                                                               cTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGcc                                        <  2:3387529/140‑1 (MQ=255)

                                                                                                   aaTATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAAt                                    <  1:5805757/140‑1 (MQ=255)

                                                                                                   aaTATAGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAAt                                    <  1:2614355/140‑1 (MQ=255)

                                                                                                    ctatTGAACAGGAGGCGGTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTGTGTCCATCCGACCGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATg                                   <  2:5223600/139‑1 (MQ=255)

                                                                                                     tatTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGc                                  >  2:170361/1‑140 (MQ=255)

                                                                                                        tGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAg                               >  1:528825/1‑140 (MQ=255)

                                                                                                         gAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGc                              <  1:10919971/140‑1 (MQ=255)

                                                                                                          aaCAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTAGTGCCAGAATAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCCAGGGCGTTTACGTTGGGCCAAATGCCAGcc                             >  2:10288607/1‑140 (MQ=255)

                                                                                                               gaggCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGt                        >  2:8397196/1‑140 (MQ=255)

                                                                                                               gaggCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGt                        >  1:3391747/1‑140 (MQ=255)

                                                                                                                 ggCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTgg                      >  1:1333367/1‑140 (MQ=255)

                                                                                                                 ggCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTgg                      >  1:8978775/1‑140 (MQ=255)

                                                                                                                 ggCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTgg                      >  1:10163207/1‑140 (MQ=255)

                                                                                                                       aTGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGAttt                >  1:8335096/1‑140 (MQ=255)

                                                                                                                       aTGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGAttt                <  1:7734816/140‑1 (MQ=255)

                                                                                                                          ccAATTTATCAGATAGACTGTCTGACTCCGGTTGTGCCTGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTgg             <  2:4905377/140‑1 (MQ=255)

                                                                                                                          ccAATTTATCAGATAGACGGTCTGACTCCGGGTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTgg             <  2:3310912/140‑1 (MQ=255)

                                                                                                                             aTTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCg          >  1:10009099/1‑140 (MQ=255)

                                                                                                                             aTTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTGTGTCCATCCGACAGCGGTATTGTTCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCg          >  2:2405709/1‑140 (MQ=255)

                                                                                                                              tttATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGt         >  2:1616483/1‑140 (MQ=255)

                                                                                                                                tATCAGATAGACGGTCTGACTCCGGTTGTGACAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGTCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTAt       <  2:10727535/140‑1 (MQ=255)

                                                                                                                                  tCAGATAGCCGGTCTGACTCCGGTTGTGCCAGAAGAGAGTATTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCg     <  1:6082217/140‑1 (MQ=255)

                                                                                                                                  tCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCg     <  1:6366375/140‑1 (MQ=255)

                                                                                                                                  tCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCg     <  1:2329732/140‑1 (MQ=255)

                                                                                                                                  tCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCg     <  1:8169487/140‑1 (MQ=255)

                                                                                                                                  tCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCg     >  2:8932370/1‑140 (MQ=255)

                                                                                                                                  tCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCg     <  1:1895494/140‑1 (MQ=255)

GCGCCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGG  >  NZ_CP025268/1469171‑1469443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`