Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 1,572,104 C→T intergenic (‑66/+219) CXP41_RS24370 ← / ← digH protein YddY/glycoside hydrolase family 10 protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252681,572,1040CT99.8% 1725.1 / NA 448intergenic (‑66/+219)CXP41_RS24370/digHprotein YddY/glycoside hydrolase family 10 protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (248/199);  minor base G (1/0);  total (249/199)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 448 total aligned reads displayed.

ACCCTTATAAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCCACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAA  >  NZ_CP025268/1571967‑1572240
                                                                                                                                         |                                                                                                                                        
acCCTTATAAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTAc                                                                                                                                        >  2:4733122/1‑140 (MQ=255)
acCCTTATAAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTAc                                                                                                                                        >  2:1105217/1‑140 (MQ=255)
acCCTTATAAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTAc                                                                                                                                        >  2:1572524/1‑140 (MQ=255)
   cTTATAAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGt                                                                                                                                     >  2:1760819/1‑140 (MQ=255)
    ttATAAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTANCTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGtt                                                                                                                                    >  1:5990276/1‑140 (MQ=255)
     tataAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAATGAAATAAGCGATTTAGGACAGTTTCATTCTACGCTACTGttc                                                                                                                                   >  2:8956907/1‑140 (MQ=255)
     tataAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGttc                                                                                                                                   >  1:3792606/1‑140 (MQ=255)
     tataAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGttc                                                                                                                                   >  1:11055633/1‑140 (MQ=255)
     tataAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTATCAATCTACGCTACTGttc                                                                                                                                   >  2:11129302/1‑140 (MQ=255)
     tataAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGATTCAATCTACGCTACTGttc                                                                                                                                   >  1:8786955/1‑140 (MQ=255)
      ataAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGttct                                                                                                                                  >  2:3202951/1‑140 (MQ=255)
       taAGGCTGGGAAATCAGACTGAATCAAAATCAAACGCAACGTGCGAGATCGACTAACTCCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGttctt                                                                                                                                 <  2:4452232/140‑1 (MQ=255)
            cTGTGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaa                                                                                                                            <  2:7067829/140‑1 (MQ=255)
            cTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCTACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaa                                                                                                                            <  1:6696961/140‑1 (MQ=255)
            cTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAGATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaa                                                                                                                            >  2:3079929/1‑140 (MQ=255)
            cTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAATCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaa                                                                                                                            >  2:4116031/1‑140 (MQ=255)
            cTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaa                                                                                                                            >  1:2612373/1‑140 (MQ=255)
            cTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaa                                                                                                                            >  1:6754192/1‑140 (MQ=255)
            cTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaa                                                                                                                            <  1:9995088/140‑1 (MQ=255)
            cTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaa                                                                                                                            <  1:5297121/140‑1 (MQ=255)
            cTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaa                                                                                                                            <  2:5528286/140‑1 (MQ=255)
            cTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaa                                                                                                                            >  1:4897913/1‑140 (MQ=255)
            cTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTATTCagaa                                                                                                                            >  2:792064/1‑140 (MQ=255)
              gggAAATCAGACGGAATCACAATGAACCGCAACGTGCGAGATCGACTAACGGCACCAGATTCTCCTGAAATATGACGCTATACTGCCAAGAACTAAGCGAGTTAGGACAGTGTCACTCTTCGCTACTGTTCGTCaggaga                                                                                                                          >  2:7157752/1‑140 (MQ=255)
              gggAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaagt                                                                                                                          >  1:10886348/1‑139 (MQ=255)
              gggAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaaga                                                                                                                          >  1:3283007/1‑140 (MQ=255)
              gggAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaaga                                                                                                                          >  2:1302627/1‑140 (MQ=255)
              gggAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaaga                                                                                                                          >  1:117650/1‑140 (MQ=255)
              gggAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaaga                                                                                                                          >  1:10617152/1‑140 (MQ=255)
              gggAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaa                                                                                                                            >  2:7799195/1‑138 (MQ=255)
               ggAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAagag                                                                                                                         >  2:5301920/1‑140 (MQ=255)
               ggAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACAATATTCTACTGAAATATGAAGATATAATGAAAAGAAATAAGCGATTTAGTACAGTTTAAATCTACGCTACTGTTATTCAGAatag                                                                                                                         >  2:8802493/1‑140 (MQ=255)
                 aaaTCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGta                                                                                                                       <  2:10658895/140‑1 (MQ=255)
                     cAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGc                                                                                                                   >  1:9011025/1‑140 (MQ=255)
                     cAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGc                                                                                                                   <  2:210488/140‑1 (MQ=255)
                       gACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGTTATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGccc                                                                                                                 <  2:5985002/140‑1 (MQ=255)
                       gACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGccc                                                                                                                 >  1:6874870/1‑140 (MQ=255)
                                          gCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGt                                                                                              <  2:3313817/140‑1 (MQ=255)
                                           ccaCGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTg                                                                                             <  1:10816451/138‑1 (MQ=255)
                                           cAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTg                                                                                             <  1:1700681/140‑1 (MQ=255)
                                           cAACGTGCCAGATCGACTAACTGCACCAGATTCTCCTGAAATATGACGATATCCTGAAAAGAAATAAGCGATTTAGGACAGTTTCAAACTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTg                                                                                             <  1:7441940/140‑1 (MQ=255)
                                            aaCGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGa                                                                                            <  2:9615170/140‑1 (MQ=255)
                                                 gCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCg                                                                                       <  1:18609/140‑1 (MQ=255)
                                                  cGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGa                                                                                      <  1:3133787/140‑1 (MQ=255)
                                                  cGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGa                                                                                      <  1:9120657/140‑1 (MQ=255)
                                                   gagaTCGACTAACTGCACCATATTCTCCTGAAATATGAAGATAGACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGaa                                                                                     <  1:8609771/140‑1 (MQ=255)
                                                   gagaTCCACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGaa                                                                                     <  2:9060247/140‑1 (MQ=255)
                                                          aCTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTa                                                                              >  1:9266616/1‑140 (MQ=255)
                                                          aCTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTa                                                                              >  2:346737/1‑140 (MQ=255)
                                                          aCTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTa                                                                              <  2:602400/140‑1 (MQ=255)
                                                                 gCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCCTCGTAATTATTTTTCGGGGACAGCGAATATCGTATGGtgtt                                                                       >  2:10371253/1‑140 (MQ=255)
                                                                  cACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGttttt                                                                      >  2:2974784/1‑140 (MQ=255)
                                                                   aCCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTCTGGTTTttc                                                                     >  2:5244437/1‑140 (MQ=255)
                                                                     cATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTttcat                                                                   <  2:7516959/140‑1 (MQ=255)
                                                                      atatTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTttcata                                                                  >  2:11165701/1‑140 (MQ=255)
                                                                       tatTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTttcatct                                                                 >  2:7271153/1‑138 (MQ=255)
                                                                         tactcCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTttcatattc                                                               >  1:9699833/3‑140 (MQ=255)
                                                                          tctcCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTttcatattca                                                              <  1:5367978/140‑1 (MQ=255)
                                                                          tctaatGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGTACAGTTTCAATCTACGCTAATGTTCTTAAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTttcatattca                                                              >  2:5438636/6‑140 (MQ=255)
                                                                            tcCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTttcatattcata                                                            >  1:4641840/1‑140 (MQ=255)
                                                                              cTGAAATATGAAGATATCCTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCAGAct                                                          >  1:5128644/1‑139 (MQ=255)
                                                                              cTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACa                                                          >  2:8329886/1‑140 (MQ=255)
                                                                               tGACATATGAAGATATACTGAAAAGAAAAAAGCGATATAGGACATTTTCAATCTACGCTCCTTTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGTCAGCGACTAGCGTATGGGGTTTCTTATTGATCCag                                                         >  2:9178496/1‑139 (MQ=255)
                                                                               tGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACAt                                                         >  2:5050833/1‑140 (MQ=255)
                                                                                 aaaTATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACAttt                                                       >  2:10941139/1‑140 (MQ=255)
                                                                                 aaaTATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACAttt                                                       >  1:2385237/1‑140 (MQ=255)
                                                                                      tGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGATACTGTTCTTCAGTAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTttatt                                                  <  1:10209344/140‑1 (MQ=255)
                                                                                        aaGATATACTGAAAAGAAATAAGCGATTTATGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAg                                                <  1:3581340/140‑1 (MQ=255)
                                                                                        aaGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAg                                                <  1:641996/140‑1 (MQ=255)
                                                                                        aaGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAg                                                <  1:8121103/140‑1 (MQ=255)
                                                                                        aaGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAg                                                <  1:9471884/140‑1 (MQ=255)
                                                                                           atatACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGa                                             >  2:3839399/1‑140 (MQ=255)
                                                                                             ataCTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGAtt                                           >  2:2515222/1‑140 (MQ=255)
                                                                                              taCTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGAttt                                          >  1:1339239/1‑140 (MQ=255)
                                                                                               aCTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTa                                         >  1:2188348/1‑140 (MQ=255)
                                                                                               aCTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTa                                         >  1:3945437/1‑140 (MQ=255)
                                                                                                cttaaaaGATATAAGCTATTTAGGACATTTTCAATCTACGATACTGTTCTTCAGAAGAGTATAGCCAATCGTAATTATTTTTCGTTGACAGCGAATATAGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTAt                                        <  2:8091934/137‑1 (MQ=255)
                                                                                                  gAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATgg                                      <  2:397744/140‑1 (MQ=255)
                                                                                                  gAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATgg                                      <  2:3645101/140‑1 (MQ=255)
                                                                                                   aaaaGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGACGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGGATGGTTTTTCATATTCATACATTGTGATTAGGGATTTATGGc                                     >  1:7192431/1‑140 (MQ=255)
                                                                                                   aaaaGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGc                                     >  1:1485090/1‑140 (MQ=255)
                                                                                                    aaaGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCTGTGACAGAGAATATAGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCt                                    <  1:5602815/140‑1 (MQ=255)
                                                                                                      agaaagaaGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGt                                  <  1:7904212/140‑1 (MQ=255)
                                                                                                      aGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGt                                  <  1:2840030/140‑1 (MQ=255)
                                                                                                      aGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGt                                  <  1:5840658/140‑1 (MQ=255)
                                                                                                      aGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGt                                  <  2:9785307/140‑1 (MQ=255)
                                                                                                            aaGCGAATTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACt                            <  1:8323258/140‑1 (MQ=255)
                                                                                                               cGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAg                         >  1:10014030/1‑140 (MQ=255)
                                                                                                                  tttAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGa                                                                                      <  2:6744585/76‑1 (MQ=255)
                                                                                                                   ttAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGtgtg                     >  1:76958/1‑140 (MQ=255)
                                                                                                                   ttAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGtgtg                     <  2:10491401/140‑1 (MQ=255)
                                                                                                                            tttCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAg                         >  2:8559239/1‑127 (MQ=255)
                                                                                                                            tttCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTg            >  2:6320343/1‑140 (MQ=255)
                                                                                                                            tttCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTg            >  2:10187444/1‑140 (MQ=255)
                                                                                                                            tttCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTg            >  2:2362550/1‑140 (MQ=255)
                                                                                                                                 aTCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTta       >  2:2734770/1‑140 (MQ=255)
                                                                                                                                    tACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTtaagt    <  1:4316474/140‑1 (MQ=255)
                                                                                                                                      cGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTtaagtaa  >  2:6491944/1‑140 (MQ=255)
                                                                                                                                      cGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTtaagtaa  >  1:3663925/1‑140 (MQ=255)
                                                                                                                                      cGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTtaagtaa  >  1:7816013/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
ACCCTTATAAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCCACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAA  >  NZ_CP025268/1571967‑1572240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑