Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 1,766,333 A→G C92R (TGT→CGT)  sufD ← Fe‑S cluster assembly protein SufD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252681,766,3330AG99.9% 2722.4 / NA 781C92R (TGT→CGT) sufDFe‑S cluster assembly protein SufD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (390/390);  minor base T (1/0);  total (391/390)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 781 total aligned reads displayed.

CACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTCA  >  NZ_CP025268/1766196‑1766469
                                                                                                                                         |                                                                                                                                        
cACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCCGTGCGGGCACGTAACGcc                                                                                                                                        >  2:1071895/1‑140 (MQ=255)
 aCGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGccc                                                                                                                                       >  1:5259515/1‑140 (MQ=255)
  cGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCa                                                                                                                                      >  1:3761761/1‑140 (MQ=255)
  cGCTTTGGGCCAGGCTTTCCGTCAACGGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAACCCCTGACGGGCGTCGTTAATGCTCACTTCATCTCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCa                                                                                                                                      <  2:3902704/140‑1 (MQ=255)
   gCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCAt                                                                                                                                     >  1:8430226/1‑140 (MQ=255)
   gCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCAt                                                                                                                                     <  1:1970368/140‑1 (MQ=255)
      ttGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGa                                                                                                                                  <  1:10730461/140‑1 (MQ=255)
       tgtgCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGAc                                                                                                                                 >  2:8284052/1‑140 (MQ=255)
        gtgCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACa                                                                                                                                <  2:10151972/140‑1 (MQ=255)
        gtgCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACa                                                                                                                                <  1:6415726/140‑1 (MQ=255)
          gCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACaaa                                                                                                                              >  2:7327591/1‑140 (MQ=255)
             aGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACGTCATATCCGCTGCCGTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAacac                                                                                                                           >  2:1204111/1‑140 (MQ=255)
               gCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCa                                                                                                                         >  2:786558/1‑140 (MQ=255)
                 tttCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGc                                                                                                                       <  2:4615459/140‑1 (MQ=255)
                   tCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCg                                                                                                                     >  1:9069582/1‑140 (MQ=255)
                   tCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCg                                                                                                                     >  1:9700234/1‑140 (MQ=255)
                   tCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCg                                                                                                                     >  2:584601/1‑140 (MQ=255)
                   tCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCGTCAGTTGCATCGCTCAGGGCGGGCACGTAACGCCCATCGACCAACACCAGCCg                                                                                                                     >  2:6225797/1‑140 (MQ=255)
                     cGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCa                                                                                                                   >  1:7557676/1‑140 (MQ=255)
                      gTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCAc                                                                                                                  <  2:970950/140‑1 (MQ=255)
                      gTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCAc                                                                                                                  <  1:6871338/140‑1 (MQ=255)
                      gTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCAc                                                                                                                  <  1:1185672/140‑1 (MQ=255)
                       tCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACg                                                                                                                 <  2:3479621/140‑1 (MQ=255)
                       tCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACg                                                                                                                 <  1:6975454/140‑1 (MQ=255)
                       tCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACg                                                                                                                 <  1:6625059/140‑1 (MQ=255)
                             gCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCt                                                                                                           <  2:7682226/140‑1 (MQ=255)
                                      cTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAAc                                                                                                  >  1:11071312/1‑140 (MQ=255)
                                          cGCCTCTATAGCGTCGGGAAAACCCTGACGCCCGTCGTTCAAGCACACTTCATCTCCGCTGCCATCAGGACCATCGCTCAGTGCGGGCACGAAACGCCCATCGACAAACACCCCCCGCACGGAGTCTAACGTTAACGCTa                                                                                              <  2:11131408/140‑1 (MQ=255)
                                           gCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCCGTAGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTaa                                                                                             >  2:5539777/1‑140 (MQ=255)
                                            ccTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCCGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAg                                                                                            >  1:9965552/1‑140 (MQ=255)
                                            ccTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAg                                                                                            >  2:1892457/1‑140 (MQ=255)
                                                  tAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCTCGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTTAGGCATCa                                                                                      <  2:6110940/140‑1 (MQ=255)
                                                   aGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCAc                                                                                     >  2:6953470/1‑140 (MQ=255)
                                                    gcggcgGGTAACCCCTGACGGTCGTCGTTCCTGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                                                    <  2:8967395/140‑1 (MQ=255)
                                                       tCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTCAGGCATCACgctg                                                                                 >  2:6433818/1‑140 (MQ=255)
                                                       tCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctg                                                                                 <  1:11195650/140‑1 (MQ=255)
                                                       tCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCGTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTACCGCGAAGGCATCACgctg                                                                                 >  2:7116142/1‑140 (MQ=255)
                                                         gggTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctgct                                                                               <  1:1776309/140‑1 (MQ=255)
                                                          ggTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGctgctg                                                                              >  2:3591744/1‑140 (MQ=255)
                                                                 ccTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGat                                                                       <  2:4301942/140‑1 (MQ=255)
                                                                 ccTCACGGTCGTCGTTAATGCTCACTACATCACCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCCAAGGCAACACGCTGCTGTGGGGat                                                                       <  2:2721153/140‑1 (MQ=255)
                                                                     aCGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTATCGTTAACGCTAAGGCATCACGCTGCTGTGGGGATAtct                                                                   >  1:8193117/1‑140 (MQ=255)
                                                                          cgtcgtTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                                                    >  2:2288987/1‑118 (MQ=255)
                                                                          cgtcgtTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGAGATCTCTccc                                                              >  2:847242/1‑140 (MQ=255)
                                                                           gtcgtTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCNTCACGCTGCTGTGGGGATATCTCTCCCg                                                             >  1:5955885/1‑140 (MQ=255)
                                                                           gtcgtTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCg                                                             >  2:3198644/1‑140 (MQ=255)
                                                                           gtcgtTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCg                                                             <  2:9465337/140‑1 (MQ=255)
                                                                               ttAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAAt                                                         >  1:9142017/1‑140 (MQ=255)
                                                                               ttAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAAt                                                         >  2:10306370/1‑140 (MQ=255)
                                                                                 aaTGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGc                                                       >  2:9287859/1‑140 (MQ=255)
                                                                                   tGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCa                                                                                                                                      >  1:10513602/1‑59 (MQ=255)
                                                                                   tGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAac                                                                                                                             >  2:2575055/1‑68 (MQ=255)
                                                                                   tGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                                                    >  1:8849958/1‑109 (MQ=255)
                                                                                       cACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTTCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaa                                                 >  1:2205711/1‑140 (MQ=255)
                                                                                       cACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaa                                                 >  1:9480926/1‑140 (MQ=255)
                                                                                        aCTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaaa                                                >  2:5390237/1‑140 (MQ=255)
                                                                                          ttCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACt                                              <  2:9887019/140‑1 (MQ=255)
                                                                                          ttCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACt                                              <  1:3203971/140‑1 (MQ=255)
                                                                                          ttCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACt                                              <  1:1619079/140‑1 (MQ=255)
                                                                                               atCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTa                                         <  1:3876290/140‑1 (MQ=255)
                                                                                               atCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTa                                         >  1:428814/1‑140 (MQ=255)
                                                                                                   gCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTAATGa                                     <  2:175666/140‑1 (MQ=255)
                                                                                                    cTGCCTTCCGTTGCATCGCTCAGAGCGAGCACGAAACGCCAAACGACAAAAACCAGCCGCACGGAGTCTAACTTTAACGCTAAGCCCTCACGCTCCAGAGTGCATAACTCTCCCGCAATTCTGACAAACAGGCAAATGag                                    >  2:10564740/1‑139 (MQ=255)
                                                                                                    cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACCAACACCAGCCGCACGGAGTCTCACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATCTCTCTCCCGCAATGCTGACAAACTGTCTCTTGAt                                    >  2:7492339/1‑140 (MQ=255)
                                                                                                    cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGAt                                    >  1:2687943/1‑140 (MQ=255)
                                                                                                    cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGAt                                    >  1:2844444/1‑140 (MQ=255)
                                                                                                    cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGAt                                    >  1:10154257/1‑140 (MQ=255)
                                                                                                    cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGAt                                    >  2:875109/1‑140 (MQ=255)
                                                                                                    cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGAt                                    >  2:4543349/1‑140 (MQ=255)
                                                                                                    cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCAGTGTGGATATCTCTCCCGCACTGCTGACAAACTGGCTATTGAt                                    >  2:4162763/1‑140 (MQ=255)
                                                                                                    cTGCCTTCAGTTGCATCGCTCAGAGCGGGCAGGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAGCGCTAAGGCATCACGCTGCTGTGGGGATCTCTCTCCCGCAATGCTGACAAACTGGCTCTTGAt                                    >  2:2910233/1‑140 (MQ=255)
                                                                                                     tGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATc                                   <  1:7207919/140‑1 (MQ=255)
                                                                                                        cTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGc                                <  1:10093033/140‑1 (MQ=255)
                                                                                                            aGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCtt                            >  1:2391460/1‑140 (MQ=255)
                                                                                                              ttGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTcc                          <  2:9103950/140‑1 (MQ=255)
                                                                                                               tGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCa                         <  1:7909078/140‑1 (MQ=255)
                                                                                                               tGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCa                         >  2:6602973/1‑140 (MQ=255)
                                                                                                                 cATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAgc                       >  1:5093800/1‑140 (MQ=255)
                                                                                                                  aTCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAgcg                      <  1:3590383/140‑1 (MQ=255)
                                                                                                                  aTCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAgcg                      <  2:2000033/140‑1 (MQ=255)
                                                                                                                   tCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTCACGTTCACGCTAAGGCATCTCGCTGCTGTGGGGATCTCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAgcgg                     >  2:1455777/1‑140 (MQ=255)
                                                                                                                   tCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAGACTGGCTATTGATCAGCCCTTCCAgcgg                     >  1:10342588/1‑140 (MQ=255)
                                                                                                                     gCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAgcggcg                   <  2:10979464/140‑1 (MQ=255)
                                                                                                                      cTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGt                  >  1:1493981/1‑140 (MQ=255)
                                                                                                                      cTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGt                  >  1:4827893/1‑140 (MQ=255)
                                                                                                                      cTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGt                  >  1:8337420/1‑140 (MQ=255)
                                                                                                                      cTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGAAGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTAATGATCAGCCCTTCCAGCGGCGt                  >  1:11029578/1‑140 (MQ=255)
                                                                                                                        cAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGtat                >  1:6216717/1‑140 (MQ=255)
                                                                                                                           tGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATAtt             >  1:4483692/1‑140 (MQ=255)
                                                                                                                           tGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATAtt             >  2:501609/1‑140 (MQ=255)
                                                                                                                               ggCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCa         <  2:4805561/140‑1 (MQ=255)
                                                                                                                                gCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAg        >  1:4092183/1‑140 (MQ=255)
                                                                                                                                gCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAg        >  1:5451441/1‑140 (MQ=255)
                                                                                                                                gCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAg        >  2:9540162/1‑140 (MQ=255)
                                                                                                                                gCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAg        >  1:7440233/1‑140 (MQ=255)
                                                                                                                                     tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTc   >  1:4659914/1‑140 (MQ=255)
                                                                                                                                     tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTGCCAGCGGCGTATAGTTCCAGTTTTc   >  1:2609430/1‑140 (MQ=255)
                                                                                                                                     tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAAAGTTAACGCTAAGGCATCACGCTGCTGTGGGGAGATCTCTACCTCAATGCTGACAAAATGGCTATTGATAAGCCCTTCCAGCGGATGATATTTCCAGTTTTc   >  2:1602459/1‑140 (MQ=255)
                                                                                                                                     tAACGCCCATCGACAAACACCAGACGCACGGAGTCTAACGTTAACGATAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGAAAAACTGGGGATTGATAAGCCCTTCCAGCGGCGTATATTTCCAGATTTc   >  2:6758552/1‑140 (MQ=255)
                                                                                                                                      aaCGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTCa  <  1:5657158/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
CACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTCA  >  NZ_CP025268/1766196‑1766469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑