BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,514,921	A→G	419 (TAA→TAG)	yfeO →	ion channel protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,514,921	0	A	G	99.5%	2719.6 / NA	785	419 (TAA→TAG)	yfeO	ion channel protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (419/361); minor base T (0/4); total (419/366)
Fisher's exact test for biased strand distribution p-value = 4.66e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 785 total aligned reads displayed.

GCGGCAGTCGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCACTGTTTCTGG  >  NZ_CP025268/2514784‑2515057

         gTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGct                                                                                                                               <  2:8702568/140‑1 (MQ=255)

         gTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGct                                                                                                                               <  1:2981241/140‑1 (MQ=255)

         gTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATAGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGct                                                                                                                               <  2:7512212/140‑1 (MQ=255)

          ttGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAACAATGctc                                                                                                                              >  2:7137216/1‑140 (MQ=255)

           tGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGctct                                                                                                                             >  2:409508/1‑140 (MQ=255)

               cccAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCtt                                                                                                                         <  2:8327232/140‑1 (MQ=255)

               cccAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCtt                                                                                                                         <  1:9263515/140‑1 (MQ=255)

               cccAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCtt                                                                                                                         <  1:1840099/140‑1 (MQ=255)

                 cAATACCACATTGCTACCTCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTa                                                                                                                       <  1:9463828/140‑1 (MQ=255)

                 cAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTa                                                                                                                       <  1:6596141/140‑1 (MQ=255)

                 cAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTa                                                                                                                       <  1:8244663/140‑1 (MQ=255)

                  aaTGCCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAATATGCTCTCCTTTAt                                                                                                                      >  2:5597365/1‑140 (MQ=255)

                    tACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGa                                                                                                                    >  1:1673185/1‑140 (MQ=255)

                    tACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGa                                                                                                                    >  2:3377026/1‑140 (MQ=255)

                    tACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGa                                                                                                                    >  2:10147557/1‑140 (MQ=255)

                    tACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTGTATGa                                                                                                                    >  2:9551481/1‑140 (MQ=255)

                    tACCACATTGCTACCGCTGCTCCGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGa                                                                                                                    >  2:6567494/1‑140 (MQ=255)

                    tACCACATTGCGACCGCTGCTCTGTATCGTCTTGCTTCCGGCATGGCTGTTATTAGCTTCTAAGCCGCTGATGATGGTCAATGGTCCGAAGCAACAGCCACCCCACGATAACGTTTTTCAAAAAATGCTCTCCTTTATGa                                                                                                                    <  1:6286883/140‑1 (MQ=255)

                       cacaTTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGAttt                                                                                                                 >  2:1189187/1‑140 (MQ=255)

                       cacaTTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGAttt                                                                                                                 >  1:1165416/1‑140 (MQ=255)

                         caTTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTaa                                                                                                               >  1:143674/1‑140 (MQ=255)

                         caTTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTGTATGAGTTaa                                                                                                               >  1:10275239/1‑140 (MQ=255)

                          aTTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAag                                                                                                              <  2:9786851/140‑1 (MQ=255)

                            tGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAagag                                                                                                            >  2:5006196/1‑140 (MQ=255)

                              cTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGtt                                                                                                          >  2:4243721/1‑140 (MQ=255)

                               tACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTa                                                                                                         <  2:39217/140‑1 (MQ=255)

                                 ccGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATg                                                                                                       >  1:2260340/1‑140 (MQ=255)

                                    ctgctCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCCCCCCACGATAACGTTTAGCAAAAAATGCTCTCCTGTATGATGTAAGAGTTATGGCg                                                                                                    >  1:5560745/1‑140 (MQ=255)

                                     tgctCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCga                                                                                                   <  1:9921812/140‑1 (MQ=255)

                                     tgctCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCga                                                                                                   <  2:3551897/140‑1 (MQ=255)

                                       ctctGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCgatg                                                                                                 >  1:5704041/1‑140 (MQ=255)

                                         ctGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCgatgat                                                                                               >  2:2369801/1‑140 (MQ=255)

                                          tGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATa                                                                                              <  2:10438738/140‑1 (MQ=255)

                                          tGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATa                                                                                              <  1:4809070/140‑1 (MQ=255)

                                          tGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGAGTTAAGAGTTATGGCGATGATa                                                                                              >  1:4326021/1‑140 (MQ=255)

                                           gTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATATAAGAGTTATGGCGATGATAc                                                                                             <  1:7783478/140‑1 (MQ=255)

                                           gTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTGATGATTTAAGAGTTATGGCGATGATAc                                                                                             <  2:5193820/140‑1 (MQ=255)

                                            tATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTATGAGTTATGGCGATGATACg                                                                                            >  2:8878812/1‑140 (MQ=255)

                                             aTCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACgg                                                                                           <  2:3003922/140‑1 (MQ=255)

                                             aTCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACgg                                                                                           <  1:7616270/140‑1 (MQ=255)

                                               cGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACgggg                                                                                         >  2:290921/1‑140 (MQ=255)

                                                 tCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCt                                                                                       >  1:1000490/1‑140 (MQ=255)

                                                 tCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCt                                                                                       >  1:3143290/1‑140 (MQ=255)

                                                 tCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATATAAGAGTTATGGCGATGATACGGGGCt                                                                                       >  2:10887685/1‑140 (MQ=255)

                                                    tGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTa                                                                                    >  1:2006743/1‑140 (MQ=255)

                                                    tGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTa                                                                                    >  2:767779/1‑140 (MQ=255)

                                                     gCTTCCGGCATGGCTGTTATTAGCAGGTTAGCCGATGATGATGGTCAATCGTCAGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTAc                                                                                   <  2:1565050/140‑1 (MQ=255)

                                                     gCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTAc                                                                                   <  1:3620891/140‑1 (MQ=255)

                                                      cTTCCGGCATGGCTGTTATTAGCCGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGGTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTAcc                                                                                  >  2:9300864/1‑140 (MQ=255)

                                                       ttCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCt                                                                                 <  1:10587560/140‑1 (MQ=255)

                                                       ttCCAGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCt                                                                                 <  1:4620896/140‑1 (MQ=255)

                                                         ccGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTcc                                                                               <  2:1657661/140‑1 (MQ=255)

                                                           ggCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTcccc                                                                             >  1:2527777/1‑140 (MQ=255)

                                                           ggCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGAAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTcccc                                                                             >  2:6797913/1‑140 (MQ=255)

                                                            gCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCg                                                                            <  2:6365960/140‑1 (MQ=255)

                                                            gCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCg                                                                            <  2:1072630/140‑1 (MQ=255)

                                                            gCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCg                                                                            <  1:4094719/140‑1 (MQ=255)

                                                                ggCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAat                                                                        <  1:7097534/140‑1 (MQ=255)

                                                                ggCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAat                                                                        >  1:8759256/1‑140 (MQ=255)

                                                                  cTGTTATTATCACGTAAGCCGAAGAAGATCGACCAAAGTCCGCAACACCAGCCACCCCACTATAACGTGTAGCCCAAAATTCTCACCTTTCCGATTTAAGAGTTATGGCGAGGATACGGCGCTTTCCCTCCCCGTAatat                                                                      <  2:6311038/140‑1 (MQ=255)

                                                                           gCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAAc                                                             >  1:2084428/1‑140 (MQ=255)

                                                                           gCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAAc                                                             <  1:5818150/140‑1 (MQ=255)

                                                                            cAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACa                                                            >  2:9130987/1‑140 (MQ=255)

                                                                            cAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACa                                                            >  2:699621/1‑140 (MQ=255)

                                                                             aGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAg                                                           <  1:3389091/140‑1 (MQ=255)

                                                                               gTAAGCCGATGATGATGGTCAATCGTCCGAAGCACCAGCCACCCCACGATAACGTTTAGCACAAAATGCTCTCCTTTATGATTTAAGAGTTCTGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGGTAACAGGc                                                         >  2:10073074/1‑140 (MQ=255)

                                                                               gTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTCTGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATCTTGCGGTAACAGGc                                                         >  2:1290686/1‑140 (MQ=255)

                                                                               gTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGc                                                         >  1:9043749/1‑140 (MQ=255)

                                                                               gTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGc                                                         >  1:10765664/1‑140 (MQ=255)

                                                                                      gatgatgatGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGAc                                                  <  2:2076400/140‑1 (MQ=255)

                                                                                         gatgatgGTCAATCGTCCGAAGCAACCGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGAGTTAAGAGTTATGGCGCTGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAGCTGGCCGCTGACGGc                                               >  2:2597204/1‑140 (MQ=255)

                                                                                         gatgatgGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACTGGGATTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGc                                               >  1:8129257/1‑140 (MQ=255)

                                                                                         gatgatgGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGc                                               >  1:2365015/1‑140 (MQ=255)

                                                                                              tgGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGa                                          >  1:535549/1‑140 (MQ=255)

                                                                                                     tCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGac                                   >  1:7955997/1‑139 (MQ=255)

                                                                                                      cGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGTCCGCTGACGGCTTCGAGCGTGAAg                                  <  2:185528/140‑1 (MQ=255)

                                                                                                        tCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGga                                >  1:2760189/1‑140 (MQ=255)

                                                                                                        tCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGga                                >  2:10246317/1‑140 (MQ=255)

                                                                                                         ccGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGgag                               >  1:9686670/1‑140 (MQ=255)

                                                                                                         ccGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGATAACAGGCCGCTGACGGCTTCGAGCGTGAAGgag                               <  1:10450046/140‑1 (MQ=255)

                                                                                                           gAAGCAACCGCCACCCCCCGCGAACGGAGAGCAAAAAATGCTCACCTTTCTGATTTCAGAGTTATGGCGCTGATACGGGGCTTTACCTCCCCGTAATATTGCCTTACCAGGCCGCTGCCGGCTTCGAGCGAGAAGGAGaa                             <  2:4796980/140‑1 (MQ=255)

                                                                                                           gAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGaa                             <  1:10081782/140‑1 (MQ=255)

                                                                                                              gCAACAGCCACCCCACGATAACGTTTAGNAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATaa                          <  1:5963872/140‑1 (MQ=255)

                                                                                                               cAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAAc                         >  2:11163287/1‑140 (MQ=255)

                                                                                                               cAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGAGGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCGTCGAGCGTGAAGGAGACTAAc                         >  1:8447720/1‑140 (MQ=255)

                                                                                                                aaCAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACa                        <  1:3942207/140‑1 (MQ=255)

                                                                                                                aaCAGCCACCCCACGATAACGTTTACCAACAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACa                        <  1:7372265/140‑1 (MQ=255)

                                                                                                                  cAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAAt                      <  2:6118543/140‑1 (MQ=255)

                                                                                                                   aGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATg                     >  1:4442797/1‑140 (MQ=255)

                                                                                                                     ccACCCCACGATAACGTTTATCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGtt                   <  1:6178001/140‑1 (MQ=255)

                                                                                                                     ccACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTGAAGAGTTAGGGCGATGAACCGGGGCTTTACCTCCCCGTAATAGTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGtt                   <  2:8441330/140‑1 (MQ=255)

                                                                                                                            aCGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCa            <  1:11054343/140‑1 (MQ=255)

                                                                                                                            aCGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCa            <  2:3208207/140‑1 (MQ=255)

                                                                                                                            aCGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCa            <  1:1127470/140‑1 (MQ=255)

                                                                                                                             cGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCAc           <  1:498795/140‑1 (MQ=255)

                                                                                                                               aTAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCACTg         >  2:4485997/1‑140 (MQ=255)

                                                                                                                                   cGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCACTGTTTc     >  2:8124410/1‑140 (MQ=255)

                                                                                                                                    gTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCACTGTTTCt    <  2:3739495/140‑1 (MQ=255)

                                                                                                                                    gTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCACTGTTTCt    <  1:3294142/140‑1 (MQ=255)

                                                                                                                                      ttAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCACTGTTTCTgg  >  1:685234/1‑140 (MQ=255)

GCGGCAGTCGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCACTGTTTCTGG  >  NZ_CP025268/2514784‑2515057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`