Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 247,291 A→G *250* (TAA→TAG)  yafL → C40 family peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268247,2910AG99.7% 1976.3 / NA 577*250* (TAA→TAG) yafLC40 family peptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (238/335);  minor base T (1/1);  total (239/338)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 577 total aligned reads displayed.

GTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGA‑C‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCC  >  NZ_CP025268/247154‑247423
                                                                                                                                         |                                                                                                                                      
gtgtATTTGGGCGATGTGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGa                                                                                                                                      <  2:6914427/140‑1 (MQ=255)
gtgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGa                                                                                                                                      <  2:4603086/140‑1 (MQ=255)
gtgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGa                                                                                                                                      <  1:9031945/140‑1 (MQ=255)
gtgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGa                                                                                                                                      <  1:7869537/140‑1 (MQ=255)
gtgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGa                                                                                                                                      <  2:2638233/140‑1 (MQ=255)
gtgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGa                                                                                                                                      <  1:11051886/140‑1 (MQ=255)
 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑c                                                                                                                                    >  1:1267016/1‑140 (MQ=255)
 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑c                                                                                                                                    >  2:9097222/1‑140 (MQ=255)
 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑c                                                                                                                                    >  1:11006055/1‑140 (MQ=255)
 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑c                                                                                                                                    >  2:6089951/1‑140 (MQ=255)
 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGAGTTTGTAGGA‑c                                                                                                                                    >  2:2081272/1‑140 (MQ=255)
 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTNTGACGGAAGAGACGATTTTGTAGGA‑c                                                                                                                                    >  1:6799041/1‑140 (MQ=255)
 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTGTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑c                                                                                                                                    >  1:7497900/1‑140 (MQ=255)
  gtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑C‑g                                                                                                                                  >  2:5228747/1‑140 (MQ=255)
   tATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCCTTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTGTGGGCGCGCGCAGGATTTTGACGGACGAGACGAGTTTGTAGGA‑C‑gg                                                                                                                                 >  2:10511394/1‑139 (MQ=255)
   tATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGACGAGACGATTTTGTAGGA‑C‑gg                                                                                                                                 >  2:9668795/1‑139 (MQ=255)
   tATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑C‑gg                                                                                                                                 >  1:4713732/1‑139 (MQ=255)
   tATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTGTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑C‑gg                                                                                                                                 >  1:3779313/1‑139 (MQ=255)
     tttGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑C‑ggat                                                                                                                               <  1:2883329/140‑4 (MQ=255)
     tttGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑C‑ggat                                                                                                                               <  2:1841343/140‑4 (MQ=255)
       tGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑C‑ggataa                                                                                                                             <  1:1797383/140‑6 (MQ=255)
        gggCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAg                                                                                                                             >  1:11219438/1‑140 (MQ=255)
             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCCGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGtt                                                                                                                        >  1:7179664/1‑140 (MQ=255)
              tGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGttt                                                                                                                       >  1:206615/1‑140 (MQ=255)
               gggCCATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTa                                                                                                                      >  1:3110157/1‑140 (MQ=255)
                   aaTTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTAcgcc                                                                                                                  >  1:7645818/1‑140 (MQ=255)
                        aTCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCAGTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGAGTTTGTAGGA‑CGGATAAGGCGTGTACGCCGCATc                                                                                                             >  2:6458400/1‑140 (MQ=255)
                          cGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCg                                                                                                           >  1:3677366/1‑140 (MQ=255)
                           gAGTCGCCACGTACCGGCGACACCATTCGGATAAGCCGATGAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCCGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCgg                                                                                                          <  2:2484495/140‑1 (MQ=38)
                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCgg                                                                                                          <  1:9865984/140‑1 (MQ=255)
                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCgg                                                                                                          <  1:8237557/140‑1 (MQ=255)
                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCgg                                                                                                          <  1:149571/140‑1 (MQ=255)
                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCgg                                                                                                          <  1:10787847/140‑1 (MQ=255)
                           gAGTCGCCACGTACCGGCGAAACCAGTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCgg                                                                                                          <  2:40663/140‑1 (MQ=255)
                            aGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGc                                                                                                         >  1:7801945/1‑140 (MQ=255)
                               cGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTGTGACGGAAGAGACGATTTTGTAGGA‑CGGATCAGGCGTTTACGCCGCATCCGGCAGt                                                                                                      >  2:7056990/1‑140 (MQ=39)
                                      tACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTAcac                                                                                               <  2:1358083/140‑3 (MQ=255)
                                       aCCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCa                                                                                              <  1:9767889/140‑1 (MQ=255)
                                       aCCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTATCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCa                                                                                              <  2:6797440/140‑1 (MQ=255)
                                       aCCGGCGAAACCAGTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCa                                                                                              <  2:1082281/140‑1 (MQ=255)
                                        ccGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAg                                                                                             <  2:285971/140‑1 (MQ=255)
                                        ccGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTGTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAg                                                                                             <  2:5461045/140‑1 (MQ=255)
                                          ggCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGt                                                                                           >  2:3853232/1‑140 (MQ=255)
                                           gCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCgg                                                                                                          >  2:632216/1‑124 (MQ=255)
                                           gCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTg                                                                                          >  1:4209966/1‑140 (MQ=255)
                                           gCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTGTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTg                                                                                          >  1:5111149/1‑140 (MQ=255)
                                            cGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGc                                                                                         >  1:10188248/1‑140 (MQ=255)
                                            cGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGc                                                                                         >  1:5846913/1‑140 (MQ=255)
                                              acaCCCTTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCt                                                                                       <  2:7775377/138‑1 (MQ=39)
                                                    ttCGGATAAGCCGATGAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCg                                                                                 <  2:5638527/140‑1 (MQ=255)
                                                     tCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTGTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGATG‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGa                                                                                >  1:3323018/1‑140 (MQ=39)
                                                      cGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGAc                                                                               >  1:8823880/1‑140 (MQ=255)
                                                       ggATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACg                                                                              <  1:935538/140‑1 (MQ=255)
                                                       ggATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACg                                                                              <  2:2254738/140‑1 (MQ=255)
                                                           aaGCCGATTAGCCGAACCTTTCTGGCATGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTGGTAGGA‑CGGATAAGGCGTTTACGCCGCATCAGTCAGTTGGACGCAGGTGCCTGATGCGACGCTgg                                                                          <  2:10331209/140‑1 (MQ=37)
                                                           aaGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTgg                                                                          <  1:8334766/140‑1 (MQ=255)
                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         <  1:10044357/140‑1 (MQ=255)
                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         <  2:10631234/140‑1 (MQ=255)
                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         <  1:9099000/140‑1 (MQ=255)
                                                               cGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGcgcg                                                                      <  1:1593000/140‑1 (MQ=255)
                                                               cGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGcgcg                                                                      <  1:634485/140‑1 (MQ=255)
                                                                   tAGCCGAACCTTTCTGGCAGGACCATTGTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGAGTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTGGTACGCAGGTGCCTGAGGCGACGCTGGCGCGTcgt                                                                  >  2:9307364/1‑138 (MQ=37)
                                                                     gCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                <  1:10549736/140‑1 (MQ=255)
                                                                     gCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                <  1:9527662/140‑1 (MQ=255)
                                                                      ccGAACCTTTCTGGCAGGACCATTTGTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGACGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                               <  1:555244/140‑1 (MQ=37)
                                                                        gccccTTTCTGGCAGGACCATTGTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCCTCCGGCAGTTGTCCGCAGGTGCCTGATGCGACGCTGGCGCGTCTTAACAt                                                             <  2:333330/137‑1 (MQ=25)
                                                                        gAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCAt                                                             <  1:7316899/140‑1 (MQ=255)
                                                                              ttCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTAc                                                       <  1:6544969/140‑1 (MQ=255)
                                                                               tCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACg                                                      >  1:6042805/1‑140 (MQ=255)
                                                                               tCTGGCAGGACCATTTTTGGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACg                                                      >  1:2614257/1‑140 (MQ=255)
                                                                                cTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGa                                                     >  2:8910016/1‑140 (MQ=255)
                                                                                 tgtcAGGACCATTTTGTGGGCGCGAGCAGGATTTTGACGGAAGAGACGATTTTGTACGA‑CGGATAAGGCGTTTACGCAGCATCCGGCAGTTGTACGCAGGTGCCTGAAGCGACGCTTGCGCGACTTATCATGCCTACGAg                                                    <  2:4389835/137‑1 (MQ=37)
                                                                                 tGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAg                                                    <  2:7966038/140‑1 (MQ=255)
                                                                                    cAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGccc                                                 <  2:3671934/140‑1 (MQ=255)
                                                                                    cAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGccc                                                 <  2:9547678/140‑1 (MQ=255)
                                                                                    cAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGGTGTACGCAGGGGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGccc                                                 <  2:8286871/140‑1 (MQ=25)
                                                                                     aGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTATAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCcg                                                <  1:227316/140‑1 (MQ=255)
                                                                                      ggACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCcgc                                               <  1:1903804/140‑1 (MQ=255)
                                                                                      ggACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCcgc                                               <  2:1532514/140‑1 (MQ=255)
                                                                                      ggACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCcgc                                               >  1:7047297/1‑140 (MQ=255)
                                                                                        aCCATTTTTAGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGa                                             >  1:5243117/1‑140 (MQ=255)
                                                                                                 agggCGCGCGCAGGCTTTTGACGGCAGAGACGCGGTGGTAGGA‑CGGATCAGGCGTTTACGCCGCCTCCGGCAGGGGGACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATGTGCg                                    <  2:6582038/139‑1 (MQ=18)
                                                                                                  gggCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGa                                   >  2:4987675/1‑140 (MQ=255)
                                                                                                    gcgcgcgcAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGc                                 <  1:4503945/140‑1 (MQ=255)
                                                                                                       cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGc                              <  2:7383089/140‑1 (MQ=255)
                                                                                                         cgcAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCtt                            >  2:8848830/1‑140 (MQ=255)
                                                                                                         cgcAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGAGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCtt                            <  1:2768239/140‑1 (MQ=255)
                                                                                                            agtaTTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATTAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTATTATCATGACTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                         <  2:8590028/137‑1 (MQ=25)
                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                         <  2:1009541/140‑1 (MQ=255)
                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                         <  1:8053175/140‑1 (MQ=255)
                                                                                                                ttttGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGat                     <  2:1152408/140‑1 (MQ=255)
                                                                                                                 tttGACGGAAGAGACGATTTTGTAGGA‑CGGATAAGGCGGTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGGCTTATCATGCCTCCGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGata                    >  1:477282/1‑140 (MQ=25)
                                                                                                                   tGACGGAAGAGACGATTATGTAGGA‑CGGATAAGGCGTTTACGCCGCAACCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGACTATATGCGAGCCGCTTTCCCGAtata                  >  2:9238331/1‑140 (MQ=25)
                                                                                                                       ggAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaac              <  1:2437900/140‑1 (MQ=255)
                                                                                                                       ggAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaac              <  2:3083194/140‑1 (MQ=255)
                                                                                                                        gAAGAGACGATTTTGTTGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGTCGCTGGCGCGGCGTATCATGCCTACGAGCCCGGGTAGATTTGCGAGCCGCTTTCCCGTTATAAaaca             >  2:10249621/1‑140 (MQ=25)
                                                                                                                        gAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCtt                                                                  >  1:3969607/1‑87 (MQ=18)
                                                                                                                        gAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaaca             >  1:8697236/1‑140 (MQ=255)
                                                                                                                        gAAGAGACGATTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCAACCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaaca             >  1:10457226/1‑140 (MQ=255)
                                                                                                                                 aTTTTGTAGGA‑CGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGACTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTg    <  2:4771728/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
GTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGA‑C‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCC  >  NZ_CP025268/247154‑247423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑