BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,649,037	T→C	771 (TAA→TAG)	pbpC ←	peptidoglycan glycosyltransferase PbpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,649,037	0	T	C	99.3%	1484.4 / ‑5.4	428	771 (TAA→TAG)	pbpC	peptidoglycan glycosyltransferase PbpC
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (267/158); minor base A (3/0); total (270/158)
Fisher's exact test for biased strand distribution p-value = 3.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 428 total aligned reads displayed.

ATTGTTCTGTTGTTGTCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGAAACCACC  >  NZ_CP025268/2648903‑2649173

    ttCTGTTGTTGTCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATg                                                                                                                                 <  1:10746416/140‑1 (MQ=255)

    ttCTGTTGTTGTCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATg                                                                                                                                 <  1:7097837/140‑1 (MQ=255)

     tCTGTTGTTGTCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGa                                                                                                                                >  1:7192416/1‑140 (MQ=255)

              gtCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCACCAATCAGGGACAAAACAGACTATTGCATGACAAATTTCa                                                                                                                       >  2:10201290/1‑140 (MQ=255)

              gtCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGAATATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGAAAAAACAGACTATTGCATTACAAATTTCa                                                                                                                       >  2:6272043/1‑140 (MQ=255)

                   tcccAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTAGTGAGGAAGGTAACATTTTTTTAATATAAAATGAGGTGTAGCAACAATCAGGGACGATACAGACTATTGCATGACAAATTTCACTGTc                                                                                                                  >  2:5972765/3‑140 (MQ=255)

                   tACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCACCAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTc                                                                                                                  >  1:7490433/1‑140 (MQ=255)

                   tACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTc                                                                                                                  >  1:3900819/1‑140 (MQ=255)

                      cAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCACCAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTcgcg                                                                                                               >  1:10266113/1‑140 (MQ=255)

                         cGTACGAGATGGCGCGGATTATAATGAGCCACAGGGCCGTTGACTCTTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGAtt                                                                                                            <  2:4451833/140‑1 (MQ=255)

                         cGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGGTGAAGGTACCAATTTTTTAAAATAAAATGAGTTTTAGCAACAATCTGGGACAAAACAGACTATTGCAAGACAAATTTCACTGTCGCGAtt                                                                                                            >  2:7305246/1‑140 (MQ=255)

                         cGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGTCTATTGCATGACAAATTTCACTGTCGCGAtt                                                                                                            >  1:8364347/1‑140 (MQ=255)

                         cGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGAtt                                                                                                            >  2:5444341/1‑140 (MQ=255)

                         cGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGAtt                                                                                                            >  1:7807790/1‑140 (MQ=255)

                         cGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGAGGTAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCCGGGACCAAACCGACGAGTGCAGGACACATGTCACTGTCGCGAtt                                                                                                            >  1:9482372/1‑140 (MQ=255)

                         cGTACGAGATGGCCCGGATTAAAATGACCAACAGGGCCGTGGACTATTGAGTAAGGTAACATTTTTTTACAATAAAATGAGTTTTAGCAACAGTCAGGGAAAACACAGAATATTGCATGACCAAAATCCCGGTCGCGAtt                                                                                                            >  2:688370/1‑140 (MQ=255)

                           tACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGTCAAATTTCACTGTCGCGATTTg                                                                                                          >  1:5266305/1‑140 (MQ=255)

                           tACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTg                                                                                                          >  2:1879757/1‑140 (MQ=255)

                           tACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTg                                                                                                          >  1:4185954/1‑140 (MQ=255)

                           tACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTg                                                                                                          >  2:2865409/1‑140 (MQ=255)

                           tACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTg                                                                                                          >  1:6840265/1‑140 (MQ=255)

                           tACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTg                                                                                                          >  1:6310084/1‑140 (MQ=255)

                           tACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTg                                                                                                          >  2:423330/1‑140 (MQ=255)

                             cGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACCAAACAGACTATTGCATGACAAATGTCACTGTCGCGATTTGcc                                                                                                        >  1:7357021/1‑140 (MQ=255)

                             cGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGcc                                                                                                        >  1:8984253/1‑140 (MQ=255)

                             cGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGcc                                                                                                        >  1:322978/1‑140 (MQ=255)

                             cGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGcc                                                                                                        <  2:8912907/140‑1 (MQ=255)

                             cGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGcc                                                                                                        >  2:5201288/1‑140 (MQ=255)

                             cGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTAACTGTCGCGATTTGcc                                                                                                        >  2:1459820/1‑140 (MQ=255)

                              gagaTGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGccc                                                                                                       <  1:9995089/140‑1 (MQ=255)

                                   ggCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGt                                                                                                  <  1:9618453/140‑1 (MQ=255)

                                   ggCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGt                                                                                                  >  2:5701564/1‑140 (MQ=255)

                                   ggCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGt                                                                                                  >  1:2367681/1‑140 (MQ=255)

                                     cgcgGATTATAATGAGCAACAGGGCCTATGACTATTGAGGAAGGTAACATATTTTTCAACTCAAATGAGTGTTAGCAACAATAAGGGACAAAACAGACTAAAGCATTACAAATTTCACTGTCGCGCTTTGCCCCACGTca                                                                                                >  2:5625323/1‑140 (MQ=255)

                                     cgcgGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTca                                                                                                >  1:774206/1‑140 (MQ=255)

                                     cgcgGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTca                                                                                                >  2:11061840/1‑140 (MQ=255)

                                     cgcgGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTca                                                                                                >  2:6617109/1‑140 (MQ=255)

                                     cgcgGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTca                                                                                                >  2:3050982/1‑140 (MQ=255)

                                      gcgGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTcat                                                                                               <  1:10520448/140‑1 (MQ=255)

                                         gATTATAATGAGCAACAGGGCCGTTGACTATTGATGACGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTcatcca                                                                                            >  2:9525243/1‑140 (MQ=255)

                                          aTTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTcatccat                                                                                           >  1:828388/1‑140 (MQ=255)

                                             ataATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCAc                                                                                        <  2:3785203/140‑1 (MQ=255)

                                               aaTGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCa                                                                                      >  2:7802190/1‑140 (MQ=255)

                                                 tGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGc                                                                                    <  1:4118165/140‑1 (MQ=255)

                                                   aGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa                                                                                  >  1:4657673/1‑140 (MQ=255)

                                                   aGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa                                                                                  >  2:2707867/1‑140 (MQ=255)

                                                   aGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa                                                                                  >  2:1316093/1‑140 (MQ=255)

                                                   aGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa                                                                                  >  1:8743588/1‑140 (MQ=255)

                                                   aGCAACAGGGCCGTTGACTATTGAGGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa                                                                                  >  1:1674999/1‑140 (MQ=255)

                                                    gCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTATAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAAt                                                                                 >  1:2867882/1‑140 (MQ=255)

                                                    gCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACACATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAAt                                                                                 >  1:175367/1‑140 (MQ=255)

                                                    gCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCact                                                                                 >  2:10724989/1‑138 (MQ=255)

                                                    gCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAAt                                                                                 >  1:8914614/1‑140 (MQ=255)

                                                    gCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAAt                                                                                 <  1:10000888/140‑1 (MQ=255)

                                                    gCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAAt                                                                                 <  1:2112328/140‑1 (MQ=255)

                                                    gCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCAACGTCATCCATCACCAGCAAt                                                                                 >  2:8052361/1‑140 (MQ=255)

                                                        cAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGAt                                                                             >  1:1139159/1‑140 (MQ=255)

                                                        cAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGAt                                                                             >  2:10482475/1‑140 (MQ=255)

                                                        cAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGAt                                                                             <  1:1879757/140‑1 (MQ=255)

                                                           ggCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAAt                                                                          >  2:5783069/1‑140 (MQ=255)

                                                            gCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATc                                                                         <  1:6617109/140‑1 (MQ=255)

                                                             ccGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCa                                                                        >  2:6124709/1‑140 (MQ=255)

                                                                ttGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCt                                                                     >  2:8803794/1‑140 (MQ=255)

                                                                ttGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCt                                                                     >  1:8480424/1‑140 (MQ=255)

                                                                ttGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCAAGACAAATTTCACTGTCGCGATTTGCCACACGTCATCCAACACCAGCAAATGATAAACACCt                                                                     >  2:9831573/1‑140 (MQ=255)

                                                                    cTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTAt                                                                 <  2:8174522/140‑1 (MQ=255)

                                                                      aTTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCa                                                                                                                                   >  2:8997869/1‑72 (MQ=255)

                                                                        tgatgtAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGt                                                             <  1:5050917/140‑1 (MQ=255)

                                                                        tgatgaAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGt                                                             <  1:3682043/140‑1 (MQ=255)

                                                                         gatgaAGTTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTc                                                            <  2:6783720/140‑1 (MQ=255)

                                                                          atgaAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCa                                                           >  1:5669033/1‑140 (MQ=255)

                                                                          atgaAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCa                                                           >  1:10700660/1‑140 (MQ=255)

                                                                          atgaAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCa                                                           >  1:3416524/1‑140 (MQ=255)

                                                                            gaAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCaaa                                                         <  1:521496/140‑1 (MQ=255)

                                                                             aaGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAAt                                                        >  2:4578379/1‑140 (MQ=255)

                                                                             aaGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAAt                                                        >  1:1082896/1‑140 (MQ=255)

                                                                               ggTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCACTTGATAATCACCTTTATCCGTCAAATGc                                                      >  1:11026576/1‑140 (MQ=255)

                                                                               ggTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGc                                                      >  2:8389964/1‑140 (MQ=255)

                                                                                 tAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAg                                                    >  2:3538777/1‑140 (MQ=255)

                                                                                 tAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAg                                                    >  1:4585398/1‑140 (MQ=255)

                                                                                 tAACATTATTTTAAAATAAAATGAGTGTTAGCAACAATTTGGGAGAAAACAGACTATTGCCTGACAAAGTTCACTGTCGCGATTTGACACACGTCATCCATCACCAGCAATGGATAATCAGCTTTAACCGTCacttgctg                                                    >  2:7530087/1‑133 (MQ=255)

                                                                                    cATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTAGAGGTCAGATGCAgggg                                                 >  2:2419865/1‑139 (MQ=255)

                                                                                      tttttttAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAAGTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCa                                               <  2:8687830/140‑1 (MQ=255)

                                                                                        tttttAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACg                                             >  1:593666/1‑140 (MQ=255)

                                                                                               aataaaatGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGcc                                      <  1:6689952/140‑1 (MQ=255)

                                                                                                    aatGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGt                                 <  2:7130104/140‑1 (MQ=255)

                                                                                                    aatGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGt                                 <  1:6069375/140‑1 (MQ=255)

                                                                                                    aatGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGt                                 <  1:1237445/140‑1 (MQ=255)

                                                                                                             tAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGt                                                                                                  >  1:8104231/1‑66 (MQ=255)

                                                                                                                  acaaTCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTc                   >  2:3350384/1‑140 (MQ=255)

                                                                                                                   caaTCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCg                  <  1:3060944/140‑1 (MQ=255)

                                                                                                                     aTCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGcc                <  2:3926751/140‑1 (MQ=255)

                                                                                                                      acAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCa               <  1:10553262/139‑1 (MQ=255)

                                                                                                                          ggACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCa           >  2:4816337/1‑140 (MQ=255)

                                                                                                                              aaaaCAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGaaa       >  1:9185209/1‑140 (MQ=255)

                                                                                                                              aaaaCAGACTAGTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGaac       >  2:7968985/1‑139 (MQ=255)

                                                                                                                                aaCAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGAAacc     >  2:4090683/1‑140 (MQ=255)

                                                                                                                                aaCAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGAAacc     >  2:2143616/1‑140 (MQ=255)

                                                                                                                                aaCAGACTATTGCATGACAAATGTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGGTCAGGTAACGGTTCGCCATTCAGACacc     >  2:1822984/1‑140 (MQ=255)

                                                                                                                                  cAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGAAaccac   <  1:1379436/140‑1 (MQ=255)

ATTGTTCTGTTGTTGTCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGAAACCACC  >  NZ_CP025268/2648903‑2649173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`