Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 2,773,510 A→G *274* (TAA→TAG)  yfjQ → DUF932 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,773,5100AG99.6% 2565.9 / ‑5.4 740*274* (TAA→TAG) yfjQDUF932 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (362/375);  minor base T (3/0);  total (365/375)
Fisher's exact test for biased strand distribution p-value = 1.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 740 total aligned reads displayed.

ATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCT  >  NZ_CP025268/2773373‑2773645
                                                                                                                                         |                                                                                                                                       
  tAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGt                                                                                                                                     <  1:6941878/140‑1 (MQ=255)
     gggCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTc                                                                                                                                  <  1:3059014/140‑1 (MQ=255)
      ggCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCa                                                                                                                                 <  1:11250537/140‑1 (MQ=255)
          gACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAACCACGGCTCACGCAACTGCAGTAGACGTGTCATGtt                                                                                                                             >  2:6579018/1‑140 (MQ=255)
          gACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGtt                                                                                                                             >  2:5750343/1‑140 (MQ=255)
          gACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGtt                                                                                                                             >  2:1238881/1‑140 (MQ=255)
             tCAGTGGGCGTAATGCTAAAGGCGGACGATCTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGcc                                                                                                                          <  1:4426997/140‑1 (MQ=255)
             tCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGcc                                                                                                                          <  1:8081477/140‑1 (MQ=255)
             tCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGcc                                                                                                                          <  1:1729157/140‑1 (MQ=255)
             tCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGcc                                                                                                                          <  2:7329386/140‑1 (MQ=255)
               aGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCAc                                                                                                                        <  1:7925347/140‑1 (MQ=255)
                gTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACg                                                                                                                       <  1:5846630/140‑1 (MQ=255)
                gTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACg                                                                                                                       <  1:9007177/140‑1 (MQ=255)
                 tGGGCGTAATGCTCAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACgt                                                                                                                      >  1:2160313/1‑140 (MQ=255)
                   ggCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACgttg                                                                                                                    <  1:4233271/140‑1 (MQ=255)
                    gCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCGGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGTTGGCAGTAACACTGCTAACGCTACTGCATTAGACGTTTCTTGTTGCCAAgttgt                                                                                                                   <  2:4723610/140‑1 (MQ=255)
                    gCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACgttgt                                                                                                                   <  1:7407749/140‑1 (MQ=255)
                    gCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACgttgt                                                                                                                   <  1:4654654/140‑1 (MQ=255)
                         aTGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAata                                                                                                              >  1:7531479/1‑140 (MQ=255)
                          tGCTAAAGGCGGACGAACTCATACTCGTGCCGTGTGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAatat                                                                                                             <  1:8238022/140‑1 (MQ=255)
                          tGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAatat                                                                                                             <  1:3623353/140‑1 (MQ=255)
                          tGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAatat                                                                                                             <  1:3965812/140‑1 (MQ=255)
                              caaGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCTTGCGCTGTGGGTGATGGCAGAAACACTGCACACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGa                                                                                                         <  2:2477151/139‑1 (MQ=255)
                              aaaGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGa                                                                                                         <  1:10502710/140‑1 (MQ=255)
                              aaaCGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGa                                                                                                         <  2:6981626/140‑1 (MQ=255)
                               aaGGCGGACGAACTCATCCTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCATAAACGTGCGCTGTGGGAGATGGCAGAAACACTGCTCACGCAACTGCTGTAGACGTTTCATGTTGCCACGTTGTTAAAATCGGac                                                                                                        >  2:1739418/1‑140 (MQ=255)
                               aaGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTCGACGTTTCATGTTGCCACGGTGTTAATATCGGac                                                                                                        >  1:2979250/1‑140 (MQ=255)
                                    ggACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGAcaccac                                                                                                   >  1:1995689/1‑140 (MQ=255)
                                     gACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACaccacc                                                                                                  >  2:778098/1‑140 (MQ=255)
                                      ccGAACTCAACCTCGTGCCGTGCGTGGCATGGACGGGGATGTGCAGCTTAACCGTGCGCTGAGGGTGAGGGCAGAAACACGGCTCACGCACCTGCAGGAGACGATTCATGTTGCCACGTTGATAATATCGGACACCACCt                                                                                                 <  2:10821062/139‑1 (MQ=25)
                                      aCGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCt                                                                                                 <  2:3971490/140‑1 (MQ=255)
                                      aCGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCt                                                                                                 >  1:2449914/1‑140 (MQ=255)
                                      aCGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGGTGTTAATATCGGACACCACCt                                                                                                 >  2:2100002/1‑140 (MQ=255)
                                      aCGAACTCATACTCGAGCCGTGCGTGGCATTGACGGGGAGGTGTAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCACTCGCAACTGCAGTAGCCGTTTCATGATGCCACGTTGTTAATATCGGACACCACCt                                                                                                 >  1:935592/1‑140 (MQ=21)
                                            tCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGc                                                                                           <  2:3746359/140‑1 (MQ=255)
                                            tCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGc                                                                                           <  1:7768568/140‑1 (MQ=255)
                                             cATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCa                                                                                          <  2:8137338/140‑1 (MQ=255)
                                             cATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCa                                                                                          <  2:1413309/140‑1 (MQ=255)
                                              aTACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCAt                                                                                         <  2:490197/140‑1 (MQ=255)
                                              aTACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCAt                                                                                         <  2:9112651/140‑1 (MQ=255)
                                                aCTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCg                                                                                       <  2:7839524/140‑1 (MQ=255)
                                                  tCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCt                                                                                     <  1:1035292/140‑1 (MQ=255)
                                                  tCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCt                                                                                     <  2:4077933/140‑1 (MQ=255)
                                                  tCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCt                                                                                     <  1:1201412/140‑1 (MQ=255)
                                                  tCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCt                                                                                     <  2:6791722/140‑1 (MQ=255)
                                                  tCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCt                                                                                     <  1:5023250/140‑1 (MQ=255)
                                                  tCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCt                                                                                     <  1:10011727/140‑1 (MQ=255)
                                                     tgccgtgcGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTAtg                                                                                  >  1:6465367/1‑140 (MQ=255)
                                                      gccgtgcGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTAtgt                                                                                 <  1:3872278/140‑1 (MQ=255)
                                                      gccgtgcGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTAtgt                                                                                 >  1:10786303/1‑140 (MQ=255)
                                                      gccgtgcGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTAtgt                                                                                 >  1:4777960/1‑140 (MQ=255)
                                                           gcgtgGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCg                                                                            <  2:6104534/140‑1 (MQ=255)
                                                            cgtgGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCgt                                                                           >  2:2932131/1‑140 (MQ=255)
                                                            cgtgGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCgt                                                                           >  2:8579035/1‑140 (MQ=255)
                                                            cggggCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTCGACGTTTCATGTTGCCACGTGGTTAATCTCGGACACCACCTGTCCGCATCGCTCTGTGCTCgt                                                                           >  2:7444947/4‑140 (MQ=21)
                                                             gtgGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCgtg                                                                          <  2:8318431/140‑1 (MQ=255)
                                                             gtgGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCgtg                                                                          <  1:9249487/140‑1 (MQ=255)
                                                             gtgGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCgtg                                                                          <  2:1956190/140‑1 (MQ=255)
                                                                gCATTGACGGGGATGAGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCACACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGc                                                                       <  1:5196315/140‑1 (MQ=255)
                                                                    tGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCa                                                                   >  2:2342974/1‑140 (MQ=255)
                                                                      aCGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATCTCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCatt                                                                 >  2:4325473/1‑138 (MQ=255)
                                                                      aCGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCt                                                                     >  1:9991287/1‑136 (MQ=255)
                                                                                   gCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAg                                                    <  2:4253856/140‑1 (MQ=255)
                                                                                   gCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACGGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAg                                                    <  2:33106/140‑1 (MQ=255)
                                                                                      tAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCtt                                                 >  1:8783897/1‑140 (MQ=255)
                                                                                      tAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTGTCATGTTGACACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGcgt                                                 >  2:9534539/1‑138 (MQ=255)
                                                                                       aaCCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCa                                                                                          <  1:6282904/98‑1 (MQ=255)
                                                                                        aCCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCtttt                                               >  1:8522955/1‑140 (MQ=255)
                                                                                        aCCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCtttt                                               >  2:9163325/1‑140 (MQ=255)
                                                                                        aCCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCtttt                                               >  1:4086222/1‑140 (MQ=255)
                                                                                        aCCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGGTAGAGCtttt                                               >  1:11068146/1‑140 (MQ=255)
                                                                                         ccGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTCTGTGCTCGTGTGCCTCAATCCCCCGGGTCTAGcgtgta                                              >  2:10917037/1‑135 (MQ=25)
                                                                                           gTGCGCTGTGGGTGATGGCGGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTTTGGCtttttag                                            >  2:5144701/1‑137 (MQ=25)
                                                                                           gTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAAc                                            <  2:8952873/140‑1 (MQ=255)
                                                                                           gTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAAc                                            <  2:5576546/140‑1 (MQ=255)
                                                                                           gTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAAc                                            <  1:5399907/140‑1 (MQ=255)
                                                                                           gTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAAc                                            <  1:8416988/140‑1 (MQ=255)
                                                                                            tGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTatgc                                           >  2:10276026/1‑137 (MQ=255)
                                                                                            tGCGCGGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATCTCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAAcc                                           <  1:6165363/140‑1 (MQ=255)
                                                                                              cgcTGTGGGTGATGGCAGAAACACTGCTCACGCCACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCGCCGGTGATAGCTGTGAAccgg                                         >  1:7233211/1‑138 (MQ=25)
                                                                                              cgcTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAAcccc                                         >  1:9651106/1‑140 (MQ=255)
                                                                                                cTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCg                                       >  1:3261658/1‑140 (MQ=255)
                                                                                                 tgtgGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGt                                      <  2:9751198/140‑1 (MQ=255)
                                                                                                 tgtgGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGt                                      <  1:258025/140‑1 (MQ=255)
                                                                                                 tgtgGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGt                                      <  1:9860405/140‑1 (MQ=255)
                                                                                                 tgtgGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGt                                      <  1:5924251/140‑1 (MQ=255)
                                                                                                   tgGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTa                                    >  1:4883418/1‑140 (MQ=255)
                                                                                                           ggCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGcc                                                                                                                          >  1:1475296/1‑46 (MQ=255)
                                                                                                           ggCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTGACATCTGGc                            >  2:11127643/1‑140 (MQ=255)
                                                                                                             cAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTGATAGCTTTTAGCCCCCGTTACATCTGGcgg                          >  2:6378806/1‑138 (MQ=255)
                                                                                                                      tGCTCACGCCACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCCATCCCCCGGGGATTGCGTTTACCCCCCGTTACATCTGGCTTTTGccggag                 >  1:6700891/1‑135 (MQ=25)
                                                                                                                       gCTCACGCAACTGCAGTAGACGTTTCATTTTGAAACGTTGTTAATATCTGACAACACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCACACTGTTGTAGCGTTTAGGGCCCGGTACTTCTGGCTTTTGCAGAAAt                >  1:10644335/1‑140 (MQ=255)
                                                                                                                       gCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCACGTTACATCTGGCTTTTGCAGAAAt                >  2:2638876/1‑140 (MQ=255)
                                                                                                                       gCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCACGGGTATAGCTTTTTTCCCCCGTTACATCTGGCTGTTGCAGAAAt                >  2:8052645/1‑140 (MQ=37)
                                                                                                                         tCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATaa              <  1:157907/140‑1 (MQ=255)
                                                                                                                           aCGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATaaaa            >  2:10605583/1‑140 (MQ=255)
                                                                                                                             gCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGGTATAGCTTTTAACCCCCGTTACATCTGGCGTTTGCAGAAATaaaaag          >  2:8757811/1‑139 (MQ=255)
                                                                                                                              cAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAAt         <  2:4469/140‑1 (MQ=255)
                                                                                                                                  tGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGGGATAGCTTTTAACCCCCGTGACATCTGGCTTTTGCAGAAATAAAAAATAGtg     >  2:3545311/1‑139 (MQ=255)
                                                                                                                                     aGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCt  <  2:3094173/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
ATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCT  >  NZ_CP025268/2773373‑2773645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑