BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,960,614	C→T	A192A (GCG→GCA)	ptrA ←	pitrilysin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,960,614	0	C	T	99.9%	2719.0 / NA	705	A192A (GCG→GCA)	ptrA	pitrilysin
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (344/360); minor base G (1/0); total (345/360)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 732 total aligned reads displayed.

ATTACTGTAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTCAGCAATAGCAT  >  NZ_CP025268/2960476‑2960750

     tGTAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCggg                                                                                                                                    <  1:3293275/140‑1 (MQ=255)

      gTAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGt                                                                                                                                   >  2:5461995/1‑140 (MQ=255)

      gTAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAAACGGGGTGTGCCGGGt                                                                                                                                   >  1:5308571/1‑140 (MQ=255)

       tAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCCGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGtt                                                                                                                                  >  2:1603710/1‑140 (MQ=255)

       tAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGTTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGtt                                                                                                                                  >  2:10128313/1‑140 (MQ=255)

       tAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGACAACTTTGAACCGGGGTGTGCCGGGtt                                                                                                                                  >  1:11074048/1‑140 (MQ=255)

       tAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGtt                                                                                                                                  >  1:7858/1‑140 (MQ=255)

       tAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGtt                                                                                                                                  >  1:4457072/1‑140 (MQ=255)

       tAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGtt                                                                                                                                  >  1:9475992/1‑140 (MQ=255)

       tAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGtt                                                                                                                                  >  1:9827986/1‑140 (MQ=255)

       tAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGtt                                                                                                                                  >  1:2154668/1‑140 (MQ=255)

       tAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTACTCGAGGAAATCTTTCAGATCCTGCTGCTCCGGATTACCAGTTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTATGAAACGGGGTGTGACGGGtt                                                                                                                                  >  2:9614041/1‑140 (MQ=255)

       tAAATAACCGCCTGCATCAAATTGGAGGCATAGTAATTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGTATTAGCAGGTTTGTCGCTTAAAGTTTCGAGGTGACCACAAGATATCTTTGGAACGGGGTGTGCCGGGtt                                                                                                                                  >  2:230281/1‑140 (MQ=255)

          agaaCCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAAt                                                                                                                               <  1:4894752/138‑1 (MQ=255)

          aTAACCGCCTTCATCAAATTGGCGGACTAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAAt                                                                                                                               <  1:7086842/140‑1 (MQ=255)

          aTAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAAt                                                                                                                               <  1:2641603/140‑1 (MQ=255)

               cGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGttt                                                                                                                          <  1:6373859/140‑1 (MQ=255)

               cGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGttt                                                                                                                          <  2:5874824/140‑1 (MQ=255)

                 ccTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCt                                                                                                                        >  1:8434026/1‑140 (MQ=255)

                 ccTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCt                                                                                                                        >  1:5053880/1‑140 (MQ=255)

                 ccTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCt                                                                                                                        >  2:7200879/1‑140 (MQ=255)

                 ccTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCt                                                                                                                        >  1:9686923/1‑140 (MQ=255)

                 ccTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCt                                                                                                                        >  2:4832498/1‑140 (MQ=255)

                 ccTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCt                                                                                                                        >  2:2846124/1‑140 (MQ=255)

                 ccTTCATCAAATTCGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCt                                                                                                                        >  1:5942080/1‑140 (MQ=255)

                 ccTTCATAAAATTGGCGGAATAGTACTTCTAGTGGAAATCATTCAGCGCCTGCTGCACAGGATTACAAGGTTTGTCGCTTAAAGTTTCTAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGATTAATTGTTTCt                                                                                                                        >  2:1871531/1‑140 (MQ=255)

                   ttCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTgc                                                                                                                      >  2:11048774/1‑140 (MQ=255)

                   ttCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTgc                                                                                                                      >  2:1356462/1‑140 (MQ=255)

                    tcatcaCATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTgcg                                                                                                                     <  1:6772503/140‑1 (MQ=255)

                    tcatcaCATGCGCGGAAGAGAACTTCTCGTGGAAATCTATCAGCGCCTGCTGCACCGGATTACCCGGTTGGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACATTGAACCGGGGTGTGCCGGGTTAATGGTTTCTgcg                                                                                                                     <  2:6354133/140‑1 (MQ=255)

                    tcatcaACTTGGCGGAATCGTACTTCCCGTGGACATCTTTCAGCGCCTGCTGCACCGGATGACCAGGTTTGTCGCTTACAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTgcg                                                                                                                     <  1:8085487/140‑1 (MQ=255)

                    tcatcaAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGGAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTgcg                                                                                                                     <  2:7394732/140‑1 (MQ=255)

                    tcatcaAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTgcg                                                                                                                     <  1:10230574/140‑1 (MQ=255)

                    tcatcaAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTgcg                                                                                                                     <  1:8860628/140‑1 (MQ=255)

                    tcatcaAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTgcg                                                                                                                     <  1:9271848/140‑1 (MQ=255)

                      atcaAATTGGCGGAATAGTACTTCTCGTGGAAATCATTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCt                                                                                                                   <  2:9194127/140‑1 (MQ=255)

                         aaaTTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGAc                                                                                                                <  2:3655817/140‑1 (MQ=255)

                         aaaTTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCGGAc                                                                                                                <  2:11301273/140‑1 (MQ=255)

                           aTTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCt                                                                                                              >  1:6239401/1‑140 (MQ=255)

                           aTTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCt                                                                                                              >  2:3192561/1‑140 (MQ=255)

                           aTTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCt                                                                                                              <  2:428482/140‑1 (MQ=255)

                            ttGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTAAAAGTTGCGAGTTTACCACCAGAAAACTTTGAACCGGGGTGTGACGGGTTAATGGTTTCTGAGCTGACAtg                                                                                                             >  2:10723560/1‑140 (MQ=255)

                               gCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATGACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGc                                                                                                          <  2:8154508/140‑1 (MQ=255)

                                   aaTAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATg                                                                                                      <  2:10342869/140‑1 (MQ=255)

                                      aGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATgcgc                                                                                                   <  1:653677/140‑1 (MQ=255)

                                      aGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATgcgc                                                                                                   <  2:3889714/140‑1 (MQ=255)

                                       gTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCa                                                                                                  >  2:7573906/1‑140 (MQ=255)

                                        tACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGATTAAAGTTTCGAGGTTCCCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCAt                                                                                                 >  2:5354074/1‑140 (MQ=255)

                                            tctcGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCg                                                                                             <  2:1720121/140‑1 (MQ=255)

                                             ctcGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGt                                                                                            >  2:4059496/1‑140 (MQ=255)

                                             ctcGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGt                                                                                            >  2:8615365/1‑140 (MQ=255)

                                             ctcGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGt                                                                                            >  2:6137924/1‑140 (MQ=255)

                                               cGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCa                                                                                          >  1:8646281/1‑140 (MQ=255)

                                               cGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCa                                                                                          >  1:1866712/1‑140 (MQ=255)

                                                gTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGGTTGTCGCTTAAAGTTTCGAGGTAACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCTCTGACCTGTGCCATGCGCATCCCGTCAc                                                                                         >  2:502639/1‑140 (MQ=255)

                                                  ggAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCAcgc                                                                                       >  2:6440398/1‑140 (MQ=255)

                                                       tCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTAcg                                                                                  <  1:5515021/140‑1 (MQ=255)

                                                         tttCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTAcgcg                                                                                <  2:3107225/140‑1 (MQ=255)

                                                           tCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGcc                                                                              <  1:2747035/140‑1 (MQ=255)

                                                           tCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGcc                                                                              <  1:7343217/140‑1 (MQ=255)

                                                           tCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGcc                                                                              >  1:9151872/1‑140 (MQ=255)

                                                           tCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGcc                                                                              <  2:8452188/140‑1 (MQ=255)

                                                           tCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGGTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGcc                                                                              <  2:6633558/140‑1 (MQ=255)

                                                           tCAGCGCCTGCTGCACCGGATGACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGcc                                                                              <  1:10590497/140‑1 (MQ=255)

                                                                 ccTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGtt                                                                        >  1:8208824/1‑140 (MQ=255)

                                                                  ctgctgCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTa                                                                       >  1:1523792/1‑140 (MQ=255)

                                                                   tgctgcACCGGATTACCAGGTTTGTCGCTTAAATTTTCGAGGTTACCACCAGAAAACTTTGAAACGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTaa                                                                      >  2:5665130/1‑140 (MQ=255)

                                                                     ctgcACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAAtt                                                                    <  1:6131540/140‑1 (MQ=255)

                                                                     ctgcACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAAtt                                                                    <  1:3598192/140‑1 (MQ=255)

                                                                         aCCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGc                                                                <  2:2004796/140‑1 (MQ=255)

                                                                            ggATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGtt                                                             <  2:2473529/140‑1 (MQ=255)

                                                                              aTTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCa                                                           <  1:10790996/140‑1 (MQ=255)

                                                                                  ccAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGc                                                       >  1:11277683/1‑140 (MQ=255)

                                                                                     ggTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCAtt                                                    >  1:308762/1‑140 (MQ=255)

                                                                                     ggTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCAtt                                                    >  2:7761166/1‑140 (MQ=255)

                                                                                      gTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTa                                                   <  2:11147859/140‑1 (MQ=255)

                                                                                       tttGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTAc                                                  >  1:3872585/1‑140 (MQ=255)

                                                                                       tttGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTAc                                                  >  2:1195453/1‑140 (MQ=255)

                                                                                          gTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCt                                               <  1:10464116/140‑1 (MQ=255)

                                                                                          gTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACCTTGAACCGGGGTGTGCCGTGTTATTGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCt                                               <  2:3455267/140‑1 (MQ=255)

                                                                                           tCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTc                                              >  1:29320/1‑140 (MQ=255)

                                                                                                tAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGTGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGtt                                         <  1:4089131/140‑1 (MQ=255)

                                                                                                tAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGtt                                         <  1:10079142/140‑1 (MQ=255)

                                                                                                tAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGtt                                         <  1:1644361/140‑1 (MQ=255)

                                                                                                    gTTTCGAGGTTACCACCAGATAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGc                                     <  2:8951370/140‑1 (MQ=255)

                                                                                                              tACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTg                           >  1:1170791/1‑140 (MQ=255)

                                                                                                               accaccAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGt                          >  2:9064060/1‑140 (MQ=255)

                                                                                                               accaccAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGt                          <  1:5187918/140‑1 (MQ=255)

                                                                                                                    caGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGc                     <  1:7689477/140‑1 (MQ=255)

                                                                                                                     aGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCTCGTTCGTCATATTTCTTGTCGAGCa                    <  2:9431667/140‑1 (MQ=255)

                                                                                                                      gAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCaa                   <  1:2295654/140‑1 (MQ=255)

                                                                                                                       caaaCTTTGAACCGGGCTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGGCACGCGTACGCGCCATGGTTAAATCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCaaa                  <  2:7054099/139‑1 (MQ=255)

                                                                                                                       aaaaCTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTgcg                                                                                                                     <  2:6852361/41‑1 (MQ=255)

                                                                                                                         aaCTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAgg                <  2:2711794/140‑1 (MQ=255)

                                                                                                                           cTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGtt              >  1:995766/1‑140 (MQ=255)

                                                                                                                            tttGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTc             <  2:8757885/140‑1 (MQ=255)

                                                                                                                              tGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTCAg           >  1:4753454/1‑140 (MQ=255)

                                                                                                                              tGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTCAg           >  1:5696087/1‑140 (MQ=255)

                                                                                                                                aaCCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGAGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCTGCATATTTCTTGTCGAGCAAAGGTTCAGCa         >  1:2542063/1‑140 (MQ=255)

                                                                                                                                       gtgtGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTCAGCAATAGCAt  <  2:10430760/140‑1 (MQ=255)

ATTACTGTAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTCAGCAATAGCAT  >  NZ_CP025268/2960476‑2960750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`