BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,174,183	T→C	111 (TAA→TAG)	ygiZ ←	DUF2645 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,174,183	0	T	C	99.7%	1257.2 / NA	363	111 (TAA→TAG)	ygiZ	DUF2645 family protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (106/256); minor base A (0/1); total (106/257)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 363 total aligned reads displayed.

GATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAGTGGC  >  NZ_CP025268/3174047‑3174318

gATTGTCCAGCGAATCCCCAAATTGCATGGCATGAATGTGGAATTTGGGAAGGCGGAACAAGGGGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTaa                                                                                                                                      <  2:8767452/140‑1 (MQ=255)

   tGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAg                                                                                                                                   >  2:3012160/1‑140 (MQ=255)

    ggccAGCGAATCGCCAACTTGCATTGCATTCATGTTGAATTTGGTAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTATATGAGAGCCGTTAAAACGACTCTCATAGATTTTCCTAATAGc                                                                                                                                  <  2:6332133/138‑1 (MQ=255)

    ggccAGCGAATCGCCAAATTGCATGGAATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGc                                                                                                                                  <  2:11220822/138‑1 (MQ=255)

    gTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTTAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTTTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGc                                                                                                                                  <  2:3517816/140‑1 (MQ=255)

    gTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGc                                                                                                                                  <  2:10220168/140‑1 (MQ=255)

    gTCCAGCGAATCGCCAAATGGCATGTCATGAATGTTTAATTGGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGTTAAGCTGGTAACTTCTCTGTCTATTAGAGCAGTTAAAACGACTCTCATAGATTTTACTAATAGc                                                                                                                                  <  1:7216303/140‑1 (MQ=255)

     tCCAGCGACTCGCCAAATTGCATGGCATGAATGTGGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTATGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                 <  2:9152190/140‑1 (MQ=255)

     tCCAGCGAATCGCCATATTGCAGGGCATGAATGTTTAATTTGGGAATGCGGAACAAGGTGGATTTGCGGCGATGGTAAGCTGGTACCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTACTAGCa                                                                                                                                 <  1:10002044/140‑1 (MQ=255)

     tCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACATGGTGGATTTGAGGCGAAGGTAAGCGGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                 <  2:11064099/140‑1 (MQ=255)

     tCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                 >  2:6948422/1‑140 (MQ=255)

     tCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                 <  1:4815705/140‑1 (MQ=255)

     tCCAGCGAATCGCCAAATTGCATGGAATTAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                 <  1:4685825/140‑1 (MQ=255)

     tCCAGCGAATCGCAAATTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTATATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                 <  2:2859987/140‑1 (MQ=255)

              tCGCCAAATTGCAGGGCATGAATGTTGATTTTGGGAATGCGGAACACGGTGGATTTGAGGCGAAGGTAAGCTGTTAACTTCTCTGTCTATGAGAGCCGTTAACACGACTCTCATAGATTTTACTAATAGCAAAATATaaa                                                                                                                        <  1:7730508/140‑1 (MQ=255)

                  cAAATTGCATGTCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGCGGCGAAGGTACGCTGGTAACTTCTCTGTATATGTGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGt                                                                                                                    <  2:5654356/140‑1 (MQ=255)

                  cAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGTATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGATAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGt                                                                                                                    <  2:8345112/140‑1 (MQ=255)

                   aaaTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTc                                                                                                                   <  2:9812887/140‑1 (MQ=255)

                          atggcatgATTGTTGATTTTGGGAATGCGGAACATGGTGGATTTGAGGCGATGTTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaaa                                                                                                            <  1:6059505/140‑1 (MQ=255)

                            ggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaaaaa                                                                                                          >  2:2244251/1‑140 (MQ=255)

                                 taaTGTTGAATTGGGGAACGCGGAACCAGGTGGATTTGCGGCGAAGTTAAGCTGGAAACTACTCTGTCTTTGAGAGCCGTTAACACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGccac                                                                                                     <  1:1968297/139‑1 (MQ=255)

                                 gAATGTTGATTTTGGGAATGCGGAAATAGGTGTATTTGAGGCTAAGGTATGCTGGTAACTTCTCTGTCTATTAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGccac                                                                                                     <  1:8265927/140‑1 (MQ=255)

                                       ttattttGGGAATGCGGAACACGGTGGATTTGAGGCGATGGTAAGCTGGTAACTTCTGTGTCTTTGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaacca                                                                                               <  1:1157777/136‑1 (MQ=255)

                                       tGAATTTGGGATTGCGGAACATGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTATATGTCTATGATAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaacca                                                                                               <  2:4451394/140‑1 (MQ=255)

                                       tGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaacca                                                                                               >  1:2992488/1‑140 (MQ=255)

                                       tGAATTTGGGAAGGCGGAACCAGGTGGATTTGAGGCGAAGGTACGCGGGGAACTACGCTGTATATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAACAAAGCCaccaacca                                                                                               <  1:4431640/140‑1 (MQ=255)

                                        gAATTTGGTATTGCGGAACAAGGTGGATTTGAGGCGACTGTAAGCTGGTAACTTATCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAATATATAAACCGGCCCCAATAATGACACCAACcac                                                                                              <  2:1401191/140‑1 (MQ=255)

                                        gAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac                                                                                              >  2:126580/1‑140 (MQ=255)

                                        gAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac                                                                                              >  1:5441792/1‑140 (MQ=255)

                                            tttgtaaTGCGGAACAAGGTGGATTTGTGGCTAAGGTAAGCTGGTAACTTATCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                          <  1:1653557/135‑1 (MQ=255)

                                            ttGGGAATGCGTAACAAGGTGGATTTGAGTCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                          <  2:10932376/140‑1 (MQ=255)

                                            ttGGGAATGCGGAACATGGTGTTTTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                          <  1:7970978/140‑1 (MQ=255)

                                            ttGGGAATGCGGAACAAGGTGTATTTGAGGCGATGGTAAGCTGGTAACTTCTCTGTCTATGTGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                          <  1:6179576/140‑1 (MQ=255)

                                            ttGGGAATGCGGAACAAGGTGGATTTGAGGCGATGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                          <  2:9232263/140‑1 (MQ=255)

                                            ttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                          <  1:1403327/140‑1 (MQ=255)

                                            ttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                          <  2:9354093/140‑1 (MQ=255)

                                            ttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                          <  1:644428/140‑1 (MQ=255)

                                            ttGGCAAGGCGGAACAAGGTGGATTTGAGGCGAAGTTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTAAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                          <  1:3326797/140‑1 (MQ=255)

                                             tGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                          >  1:7416297/1‑139 (MQ=255)

                                                 attGCGGAACAAGGTGTATTTGAGGCTAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGACTTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGAc                                                                                     <  1:6576750/138‑1 (MQ=255)

                                                 aaTGCGTAACATGGTGGATTTGAGGCTAAGGTAAGCTGTTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGAc                                                                                     <  1:8988996/140‑1 (MQ=255)

                                                   tGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACaa                                                                                   >  1:5170532/1‑140 (MQ=255)

                                                   tGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACaa                                                                                   >  2:2471835/1‑140 (MQ=255)

                                                     cGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaacca                                                                                               <  1:10129506/126‑1 (MQ=255)

                                                     cGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAAt                                                                                 >  1:3987746/1‑140 (MQ=255)

                                                       gAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTTTGAGAGCCTTTAAATCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATAc                                                                               <  2:7768212/140‑1 (MQ=255)

                                                         acacGGTGTATTTGCGGCGATGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAACACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcg                                                                             <  1:1114413/140‑1 (MQ=255)

                                                           atggTGGATTTGAGGCGTAGTTTAGCTGGTATCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCTTAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAACAAGCCTCCAACCCCATACCAGACAAATACcgag                                                                           <  2:7584698/138‑1 (MQ=255)

                                                           atggTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                           <  1:7997539/138‑1 (MQ=255)

                                                           acggTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAACACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                           <  2:1208236/138‑1 (MQ=255)

                                                           aaGGTGGATTTGAGGCTAAGGTAAGCTGTTAACTTCTCTGTCTATGAGAGCCTTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                           <  1:4322158/140‑1 (MQ=255)

                                                           aaGGTGGATTTGAGGCGAAGGTAAGCTGTTAACTTCTCTGTATATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                           <  1:7023427/140‑1 (MQ=255)

                                                           aaGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                           <  2:597732/140‑1 (MQ=255)

                                                           aaGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                           <  1:2610513/140‑1 (MQ=255)

                                                           aaGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                           <  1:5794826/140‑1 (MQ=255)

                                                            aGGTGGATTTGAGGCGAAGGTAAGCTTGTAACTTCTCTTTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAAACGTCCCAAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc                                                                          <  2:8475033/140‑1 (MQ=255)

                                                            aGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc                                                                          <  1:10368380/140‑1 (MQ=255)

                                                               tgtaTTTGAGGCGATGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcgag                                                                       <  1:8807563/137‑1 (MQ=255)

                                                               tGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcgag                                                                       >  2:1777201/1‑140 (MQ=255)

                                                                gtaTTTGAGGCGAAGTTAAGCTGGTAACTTCTCTGTCTATGAGAGCCTTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCgaga                                                                      <  1:316353/138‑1 (MQ=255)

                                                                 gATTTGAGTCTAAGTTCAGCTGGTAACGTCTCTGTCTAAGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCACAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa                                                                     <  2:10274530/140‑1 (MQ=255)

                                                                 gATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTGTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa                                                                     <  2:10413137/140‑1 (MQ=255)

                                                                 gATGTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGATAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa                                                                     <  2:7328614/140‑1 (MQ=255)

                                                                       aGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACata                                                               <  1:9345623/140‑1 (MQ=255)

                                                                       aGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACata                                                               >  2:11100334/1‑140 (MQ=255)

                                                                            aaGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                          >  1:2313495/1‑108 (MQ=255)

                                                                             aGGTAAGCTGTTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAAGACATATACCCa                                                         <  2:6829577/140‑1 (MQ=255)

                                                                                       gTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACata                                                               >  1:6653257/1‑124 (MQ=255)

                                                                                       gTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACata                                                               <  2:6653257/124‑1 (MQ=255)

                                                                                       gTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACata                                                               <  2:10070630/124‑1 (MQ=255)

                                                                                       gTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAAc                                               >  2:1453211/1‑140 (MQ=255)

                                                                                         aaCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACAt                                             >  2:1526416/1‑140 (MQ=255)

                                                                                             tctcTTTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTcc                                         <  1:1839155/140‑1 (MQ=255)

                                                                                             tctcTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTcc                                         <  1:538899/140‑1 (MQ=255)

                                                                                             tctcTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTcc                                         <  1:10508309/140‑1 (MQ=255)

                                                                                             tctcTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTcc                                         <  1:10962567/140‑1 (MQ=255)

                                                                                             actctGTCCATGAGAGCCTTTAAAACGACTCTCATAGAAATTACTCCTAGCAAAATAACAACCGTCCCCAAACCAGCCACCAACCACACCCCAGACAACAACAGAGCGAGACTACATCTACCCAAAACACGAACATGTcc                                         <  2:9536471/139‑1 (MQ=255)

                                                                                              ctctGTCTATGAGACCAGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCAAAAAAAGCCACCAACCAAAAACCAGACAAAGACCGAGCGAGAATACATATACCCAAAACTCGAACATTTccc                                        <  2:4122009/140‑1 (MQ=255)

                                                                                                 tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCa                                     >  1:2125245/1‑140 (MQ=255)

                                                                                                  gTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCAt                                    <  1:6328372/140‑1 (MQ=255)

                                                                                                           agCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGttt                           >  1:3436860/1‑140 (MQ=255)

                                                                                                           agCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGttt                           >  1:9911579/1‑140 (MQ=255)

                                                                                                             ccGTTAAAACGACTCTCATAGATTTTACTAATAGc                                                                                                                                  >  2:4939239/1‑35 (MQ=255)

                                                                                                             ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc                         >  1:4982291/1‑140 (MQ=255)

                                                                                                             ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc                         >  2:10756258/1‑140 (MQ=255)

                                                                                                             ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc                         >  1:4511636/1‑140 (MQ=255)

                                                                                                             ccGTTAAAACGACTCACATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc                         >  1:6356154/1‑140 (MQ=255)

                                                                                                               gTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCtt                       <  1:10783348/140‑1 (MQ=255)

                                                                                                               gTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCtt                       >  2:9650788/1‑140 (MQ=255)

                                                                                                                    aaCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagat                  <  1:8540919/140‑1 (MQ=255)

                                                                                                                     aCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACACACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagata                 <  2:349947/140‑1 (MQ=255)

                                                                                                                      cGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACACATACCGAGCGAGAATACATAGACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagataa                <  1:9933699/140‑1 (MQ=255)

                                                                                                                      cGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagataa                <  2:7037223/140‑1 (MQ=255)

                                                                                                                        aatctaATATATTTTAATAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagataaga              <  1:4201589/138‑1 (MQ=255)

                                                                                                                        aCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACCAATACCGAGCGAGAATACATAGACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagataaga              <  2:5262881/140‑1 (MQ=255)

                                                                                                                        aCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagataaga              >  1:1364540/1‑140 (MQ=255)

                                                                                                                        aCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagataaga              >  2:3791512/1‑140 (MQ=255)

                                                                                                                             cATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGaataat         <  2:4196163/140‑1 (MQ=255)

                                                                                                                              ctAGATTGTACTAACCGCACACTATAACCCGGCCCCCACAAAGCCACCAACCACAACCCAGACAAATACCGAGCGAGAAGCCATAGACCCACAACTCTCACATTGCCCGCATCAAGCGTTTTCTTAAGCTACGAataata        <  2:4571911/139‑1 (MQ=255)

                                                                                                                                    tttACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAGTGGc  >  1:9198412/1‑140 (MQ=255)

GATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAGTGGC  >  NZ_CP025268/3174047‑3174318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`