BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,179,162	T→C	141 (TAA→TAG)	yqiB ←	DUF1249 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,179,162	0	T	C	99.7%	2005.9 / NA	579	141 (TAA→TAG)	yqiB	DUF1249 family protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (300/277); minor base A (2/0); total (302/277)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 579 total aligned reads displayed.

TGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGAAGCGAA  >  NZ_CP025268/3179026‑3179298

tGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCtaa                                                                                                                                       <  2:8037628/140‑1 (MQ=255)

  tttACCCCTAACAGGGCTTCGTGCTTTTGTGCACACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCtaata                                                                                                                                     <  2:7455886/140‑1 (MQ=255)

   ttACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCtaataa                                                                                                                                    >  2:3001929/1‑140 (MQ=255)

   ttACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCtaataa                                                                                                                                    >  2:237187/1‑140 (MQ=255)

   ttACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCtaataa                                                                                                                                    >  1:7431279/1‑140 (MQ=255)

   ttACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCtaataa                                                                                                                                    >  1:4708088/1‑140 (MQ=255)

    tACCCCTTAGAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTTATTTGTAATATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCGTTAGGTTTCACGACGCTAATaaa                                                                                                                                   >  2:3578147/1‑140 (MQ=255)

     aCCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGTTAATTTGTAAAAACCTGACACAGTCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAAAAGTGTACTTAGGATTCACGAAGATAATAAAc                                                                                                                                  >  2:4389908/1‑140 (MQ=255)

     aCCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAAc                                                                                                                                  >  1:10635780/1‑140 (MQ=255)

       cccTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCg                                                                                                                                >  2:3343810/1‑140 (MQ=255)

         cTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGa                                                                                                                              <  2:8621022/140‑1 (MQ=255)

          tAACAGGGCTTCGACCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGaa                                                                                                                             >  2:6344882/1‑140 (MQ=255)

             cAGCGCATCGAGCTTTAGTGCAAACAGGTGAGAGTCGGTAATTTGTAAAATCCTGACTCGGGCCTCACAAGCCAGAGGAAGGGTTAACAGGCGTTCCAAATGGTGTCCTTAGGTTTCACGTCGCTAATAAACCGGAATCg                                                                                                                          <  2:6202899/140‑1 (MQ=255)

               gggCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGcc                                                                                                                        <  2:5973377/140‑1 (MQ=255)

               gggCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGcc                                                                                                                        <  2:4231532/140‑1 (MQ=255)

                ggCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGCAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGACTCGCCa                                                                                                                       >  1:4350215/1‑140 (MQ=255)

                ggCGTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAATTCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAATTGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGCATCGCCa                                                                                                                       >  2:4604625/1‑140 (MQ=255)

                 gCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCAt                                                                                                                      >  2:10411777/1‑140 (MQ=255)

                 gCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCAt                                                                                                                      >  1:10259842/1‑140 (MQ=255)

                  cTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATc                                                                                                                     <  1:5576781/140‑1 (MQ=255)

                   ttCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCg                                                                                                                    <  1:3993390/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTTTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                                <  2:4029295/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCTGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                                <  2:7753826/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                                <  1:2321386/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                                <  1:8538920/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                                <  1:7296371/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                                <  1:7216596/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                                <  1:7043954/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                                <  2:11048555/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                                <  1:1664959/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                                <  1:5378949/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                                <  1:1898424/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                                <  2:9160025/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCGGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                                <  2:8896248/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGACGGTAATTTGTAAAATCCTGACTCTGGCCTCCCCCGCCAGAGGAAGGGTTAACAGGCTATCCAACTGGTGTCCTTAGGTTTCACGACGCTAATCACCCGGAATCGCCATCGCAcc                                                                                                                <  2:155652/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGAGAGTGTCGGTAATTTGTAAAATCCTGACTCGGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTGTCCAACTGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                                <  2:6495451/140‑1 (MQ=255)

                          ttttGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCAtg                                                                                                             >  2:10220498/1‑140 (MQ=255)

                            ttGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCAtgtg                                                                                                           <  1:1398671/140‑1 (MQ=255)

                               tgCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTGTCACGACGCTAAGACACCGGAATCGCCAGCGCTCCATGTGCtc                                                                                                        >  1:7824243/1‑139 (MQ=255)

                                 cAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTaaa                                                                                                      <  2:2586857/140‑1 (MQ=255)

                                 cAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTaaa                                                                                                      <  2:3816344/140‑1 (MQ=255)

                                 cAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTaaa                                                                                                      >  1:8882920/1‑140 (MQ=255)

                                   aaCAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACa                                                                                                    <  2:9875655/140‑1 (MQ=255)

                                      aGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTa                                                                                                 >  1:3365759/1‑140 (MQ=255)

                                       ggTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTAt                                                                                                <  1:902256/140‑1 (MQ=255)

                                       ggTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTAt                                                                                                <  1:4505835/140‑1 (MQ=255)

                                       ggTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTAt                                                                                                <  1:11134662/140‑1 (MQ=255)

                                        gTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATc                                                                                               >  1:25737/1‑140 (MQ=255)

                                        gTGAGTGTCGGTAAGTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCGTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGACTCGCCATCGCTCCATGTGCTAAACAGTATc                                                                                               >  1:6748329/1‑140 (MQ=255)

                                         tGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCg                                                                                              >  1:3080599/1‑140 (MQ=255)

                                             tgtCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                                >  1:4443864/1‑118 (MQ=255)

                                             tgtCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAAc                                                                                          >  1:991872/1‑140 (MQ=255)

                                                 ggTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGt                                                                                      <  1:4867584/140‑1 (MQ=255)

                                                 ggTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGt                                                                                      <  1:6182713/140‑1 (MQ=255)

                                                  gTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCACCCAGTc                                                                                     >  1:635299/1‑140 (MQ=255)

                                                  gTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTc                                                                                     >  1:10001360/1‑140 (MQ=255)

                                                       ttGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTa                                                                                >  1:4104179/1‑140 (MQ=255)

                                                         gTAAAATCCTGACTCTGGCCTCACCAGCCAGAGTAAGGGTTAACAGGCTTTCCAAATGNTGTCATTAGGTTTCACGACGCTATTAAACCGGAATCGCCATCGATCCATGTGCTAAACAGTATCGCAACCAGTCCGCTaaa                                                                              >  1:5970561/1‑140 (MQ=255)

                                                          tAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTaaaa                                                                             >  2:8144671/1‑140 (MQ=255)

                                                          tAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTaaaa                                                                             >  2:1250916/1‑140 (MQ=255)

                                                                ccTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGAt                                                                       <  2:7903947/140‑1 (MQ=255)

                                                                ccTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGAt                                                                       <  1:620488/140‑1 (MQ=255)

                                                                    aCTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAAt                                                                   >  2:2025694/1‑140 (MQ=255)

                                                                    aCTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAAt                                                                   >  1:8643138/1‑140 (MQ=255)

                                                                       ctGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTg                                                                <  1:10508001/140‑1 (MQ=255)

                                                                              cACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCttt                                                         >  1:5797472/1‑140 (MQ=255)

                                                                               aCCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCtttt                                                        >  1:3789158/1‑140 (MQ=255)

                                                                                         ggAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTg                                              >  1:10709013/1‑140 (MQ=255)

                                                                                           aaGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGAt                                            >  1:11000848/1‑140 (MQ=255)

                                                                                              ggTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAACACTGATAAATTTGATGCTTTTCGTCGCGTTGATGCa                                         >  2:921813/1‑140 (MQ=255)

                                                                                                ttAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAAc                                       <  2:10035839/140‑1 (MQ=255)

                                                                                                  aaCAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACtt                                     >  1:8174235/1‑140 (MQ=255)

                                                                                                      ggCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCCTGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTtta                                 <  1:9794404/140‑1 (MQ=255)

                                                                                                       gCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTttat                                <  1:5149741/140‑1 (MQ=255)

                                                                                                       gCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTttat                                <  2:9300788/140‑1 (MQ=255)

                                                                                                        cTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTttatt                               >  2:7290901/1‑140 (MQ=255)

                                                                                                        cTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTttatt                               >  1:7723137/1‑140 (MQ=255)

                                                                                                          ttCCAAATGGTTTACTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTGCATGTGCTAAACAGTATCGCAACCAGTCCGCTTAAATCTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                             <  2:5685457/140‑1 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                             <  2:7094359/140‑1 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                             <  1:10076385/140‑1 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                             >  2:5443042/1‑140 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                             <  2:5115705/140‑1 (MQ=255)

                                                                                                            ccAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGCTTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGa                           <  1:9597257/140‑1 (MQ=255)

                                                                                                            ccAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGa                           <  1:9244895/140‑1 (MQ=255)

                                                                                                            ccAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGa                           <  1:8301031/140‑1 (MQ=255)

                                                                                                            ccAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGa                           <  2:5866577/140‑1 (MQ=255)

                                                                                                            ccAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGa                           <  2:10860344/140‑1 (MQ=255)

                                                                                                                 tGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTCTCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATc                      >  1:3272274/1‑140 (MQ=255)

                                                                                                                  ggTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGGTGATGCAACTTTTTATTAGGATAATCa                     >  2:1918417/1‑140 (MQ=255)

                                                                                                                   gtgtCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCAt                    >  2:487931/1‑140 (MQ=255)

                                                                                                                     gtCCTTAGGGTTCACGACGCTAATAAACCGGAATCTCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAAATGATTAATTTGATGCTTTTCGACGCGTTGATGCAACTTTTTATTAGGATAATCATAc                  >  2:2893541/1‑140 (MQ=255)

                                                                                                                      tCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCTTGTGCTATACAGTATCGCAACCAGTCCGCTAAAAAGTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATaac                 >  2:2192590/1‑138 (MQ=255)

                                                                                                                      tCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGTTGCAACTTTTTATTAGGATAATCATAcc                 >  2:9561425/1‑140 (MQ=255)

                                                                                                                      tCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATAcc                 <  1:3523460/140‑1 (MQ=255)

                                                                                                                           aGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCt            >  2:8385216/1‑140 (MQ=255)

                                                                                                                           aGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCt            >  1:11302215/1‑140 (MQ=255)

                                                                                                                                tCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGaa       >  2:6694644/1‑140 (MQ=255)

                                                                                                                                tCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGaa       >  1:8798928/1‑140 (MQ=255)

                                                                                                                                tCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGaa       >  1:4629767/1‑140 (MQ=255)

                                                                                                                                 cACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGAAg      >  1:2836657/1‑140 (MQ=255)

TGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGAAGCGAA  >  NZ_CP025268/3179026‑3179298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`