BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,255,705	A→G	234 (TAA→TAG)	yhaK →	pirin family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,255,705	0	A	G	99.8%	2132.0 / NA	611	234 (TAA→TAG)	yhaK	pirin family protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (375/235); minor base T (0/1); total (375/236)
Fisher's exact test for biased strand distribution p-value = 3.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 612 total aligned reads displayed.

AAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCTGGAAGCT  >  NZ_CP025268/3255568‑3255841

  aTTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGt                                                                                                                                      >  1:11010741/1‑140 (MQ=255)

  aTTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGt                                                                                                                                      >  1:1221557/1‑140 (MQ=255)

         gcgcTTACGCATCATGAAGAGAACGCGGCGCTGACCTGCGGTGATGGGGCGGTTATTCGTGACGAGGCGAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTAct                                                                                                                               >  2:2219059/1‑139 (MQ=255)

          cgcTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAg                                                                                                                              <  2:7519163/140‑1 (MQ=255)

          cgcTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAg                                                                                                                              <  2:1338692/140‑1 (MQ=255)

                      atGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAgatgatgat                                                                                                                  <  1:4824798/140‑1 (MQ=255)

                      atGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAgatgatgat                                                                                                                  >  2:3585179/1‑140 (MQ=255)

                       tGAAGAGAAAGCGGCGCTGACCTGCGGTGTTGGGGCGTTTATTCGTGACGAGGCTCACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGAGAGATTTGCCTGTCTAGATGTGGTTCCAGGGAGatgaggatg                                                                                                                 >  2:4570586/1‑140 (MQ=255)

                       tGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGGTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGatgatgatg                                                                                                                 >  1:2782254/1‑140 (MQ=255)

                             gaAAGCGGCGCTGACCTTCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGAGCGCTATGATTATAGATTTGCCTGTCTAGATGTGTTTAAAGGGCGATGAAGATGAGGaaa                                                                                                           >  2:7671006/1‑140 (MQ=255)

                             gaAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTaaa                                                                                                           >  2:1827629/1‑140 (MQ=255)

                               aaGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTaaaaa                                                                                                         >  1:5584554/1‑140 (MQ=255)

                                 gcggcgCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAAt                                                                                                       <  2:1676776/140‑1 (MQ=255)

                                     cgcTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGc                                                                                                   >  2:2467092/1‑140 (MQ=255)

                                            cTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAg                                                                                            >  2:5714894/1‑140 (MQ=255)

                                               cGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAgcgc                                                                                         >  1:9872260/1‑140 (MQ=255)

                                                ggggttGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGTTTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGcc                                                                                        <  2:9520843/135‑1 (MQ=255)

                                                ggTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGcc                                                                                        <  1:1088899/140‑1 (MQ=255)

                                                ggTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGcc                                                                                        <  1:10774885/140‑1 (MQ=255)

                                                ggTGAAGGGGCGTTTATTCGTGACGCGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCAGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGcc                                                                                        <  1:7523942/140‑1 (MQ=255)

                                                  tGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAg                                                                                      <  2:8227361/140‑1 (MQ=255)

                                                    aTGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGcc                                                                                    >  1:1832287/1‑140 (MQ=255)

                                                    aTGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGcc                                                                                    >  2:5124644/1‑140 (MQ=255)

                                                    aTGGGGCGTTTAGTCGTGACGAGGATAACCTTCCGCTGGTTGCCGTTTCCCCACTGCGCGCTGTGCTGATAGATTTGCCTGTCTAGTTGTTTATACTGGGAGATGAGGATGAGTGAAAAATTGTCCAAAAAGCGCAAGcc                                                                                    >  2:2869295/1‑140 (MQ=255)

                                                         gCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGAGGAGTAATAAATTGGCCAAAAAGCGCCAGCCGGGGa                                                                               >  1:2027302/1‑140 (MQ=255)

                                                          cGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGaa                                                                              >  1:2533284/1‑140 (MQ=255)

                                                          cGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCAATGCGCGCTTTGCTGATAGATTTGCATGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAATAATTGGCCAAAAAGCGCCAGCCGGTGaa                                                                              >  2:96319/1‑140 (MQ=255)

                                                           gTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAg                                                                             <  2:9360137/140‑1 (MQ=255)

                                                            tttATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAg                                                                             >  2:1550393/1‑139 (MQ=255)

                                                                    tGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCgtggtg                                                                    <  1:6344264/140‑1 (MQ=255)

                                                                       cGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCg                                                                 <  2:6549221/140‑1 (MQ=255)

                                                                        gAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGa                                                                <  2:3329663/140‑1 (MQ=255)

                                                                         aGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGaa                                                               <  1:1461037/140‑1 (MQ=255)

                                                                         aGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGaa                                                               >  1:2219613/1‑140 (MQ=255)

                                                                           gcgaaCCTTACGCTGCTGGCCGCCTCCCCCCGCCGCGCAGGGCTGATAGATTTGCCTGTCGAGTTGATTTTCCAGGGAGAGGATGAAGACTAACAAATTGGCCAAAACGCGCCAGCCGGTGAAGCCCCTCGTGGCGCAAg                                                             <  2:5525607/137‑1 (MQ=255)

                                                                           gCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAg                                                             <  1:9533462/140‑1 (MQ=255)

                                                                                     cGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTACAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCg                                                   >  1:3191331/1‑140 (MQ=255)

                                                                                     cGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCg                                                   >  1:6140579/1‑140 (MQ=255)

                                                                                     cGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCg                                                   >  1:1597716/1‑140 (MQ=255)

                                                                                     cGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCg                                                   >  2:10280020/1‑140 (MQ=255)

                                                                                     cGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCg                                                   >  2:8689280/1‑140 (MQ=255)

                                                                                     cGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCg                                                   >  2:6226175/1‑140 (MQ=255)

                                                                                     cGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCg                                                   >  1:8254729/1‑140 (MQ=255)

                                                                                     cGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCg                                                   >  2:5387108/1‑140 (MQ=255)

                                                                                     cGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGGGGTGGCGAAAGATCCTGCTCg                                                   >  1:6821016/1‑140 (MQ=255)

                                                                                     cGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGAGGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGACCGTGGTGGCGAAAGAACCTGCTCg                                                   >  2:1014584/1‑140 (MQ=255)

                                                                                         ggTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCAcc                                               >  2:7778104/1‑140 (MQ=255)

                                                                                          gTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTCAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCg                                              >  1:7366230/1‑140 (MQ=255)

                                                                                          gTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCg                                              >  2:2367429/1‑140 (MQ=255)

                                                                                          gTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCg                                              >  2:6818288/1‑140 (MQ=255)

                                                                                          gTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGGAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCg                                              >  1:4518119/1‑140 (MQ=255)

                                                                                           ttGCCGATTCCCCCCTGCGCGCTTTGCTGATCGATTTGCCTGTCTAGTGGTTGTTCCAGGGAGATGAGGGTGAGTAAATCATTGGCCACAAAGCGCCAGCCGGTGAAGCCCGAGGTGGCGAAAGAACCTGCGCGcgccgc                                             >  2:11252623/1‑140 (MQ=255)

                                                                                           ttGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGc                                             >  2:10469209/1‑140 (MQ=255)

                                                                                             gCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTCGTTGTTTTTACAGGGAGATGATGAGGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAATGAACCTGCTCGCACCGCCa                                           >  1:7016049/1‑140 (MQ=255)

                                                                                             gCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCa                                           >  1:3435961/1‑140 (MQ=255)

                                                                                                    ccccACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCCAAAAttt                                    >  1:4615046/1‑140 (MQ=255)

                                                                                                    ccccACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAAttt                                    >  1:9288005/1‑140 (MQ=255)

                                                                                                     cccACTGCGCGCGTAGCTGATAGATTTGCCTGTCGAGTTGTTTTTACAGGGAGATGATGATGAGTAAACAATGGGCCAAAAAGCGCCAGCCGGTGATGCCTGTTGTGGCGAAAGAACCTGCTCGCACAGCCaaaaagtgt                                   >  2:7889083/1‑136 (MQ=255)

                                                                                                       cACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTgg                                 <  2:6054119/140‑1 (MQ=255)

                                                                                                       cACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTgg                                 >  2:2983517/1‑140 (MQ=255)

                                                                                                         ccgcgcgcTTTGCTGATAGATTGGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCt                               <  1:10240949/138‑1 (MQ=255)

                                                                                                         cTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCt                               <  1:375088/140‑1 (MQ=255)

                                                                                                         cTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCt                               <  1:8739989/140‑1 (MQ=255)

                                                                                                           gcgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCTCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTAt                             >  2:9771155/1‑140 (MQ=255)

                                                                                                           gcgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCt                               >  1:4207269/1‑138 (MQ=255)

                                                                                                           gcgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCt                               <  2:4206669/138‑1 (MQ=255)

                                                                                                            cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATTATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTTGCTAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            <  2:4453421/140‑1 (MQ=255)

                                                                                                            cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGACGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            >  1:9734284/1‑140 (MQ=255)

                                                                                                            cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            >  1:5750752/1‑140 (MQ=255)

                                                                                                            cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            >  1:7579575/1‑140 (MQ=255)

                                                                                                            cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            >  1:11259438/1‑140 (MQ=255)

                                                                                                            cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            >  2:8542054/1‑140 (MQ=255)

                                                                                                            cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            >  2:9205880/1‑140 (MQ=255)

                                                                                                            cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            >  2:3211389/1‑140 (MQ=255)

                                                                                                            cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            >  2:3090166/1‑140 (MQ=255)

                                                                                                            cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            >  1:10100041/1‑140 (MQ=255)

                                                                                                            cgcgcTTGGCTGATAGGTTGGGGTGTCTAGTTGTTTTTAAAGGGAGATGTTGATGAGTAAAAAAGTGGCCCTAATGCGCCAGCGGGTGAAGCCCGTGGTGGCGCAAGAACCTGCGCGCGCCGCCAGAAAGTTGGGCTATg                            >  2:3937016/1‑140 (MQ=255)

                                                                                                             gcgcTTTGCTGATAGATTTGACTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCAGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGa                           >  2:525273/1‑140 (MQ=255)

                                                                                                              cgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAATAACCTGCTCGCACCGCCAAAAATTTTGGCTATGaa                          <  2:489583/140‑1 (MQ=255)

                                                                                                              cgcTTTGCTGATAAATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTAGCACCGCCAAAAATTTTGGCTATGaa                          >  1:5937665/1‑140 (MQ=255)

                                                                                                              cgcCTTGCTGATAGATTTGCCTGTATAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGaa                          <  2:7058880/140‑1 (MQ=255)

                                                                                                               gcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAag                         >  2:3651234/1‑140 (MQ=255)

                                                                                                                  ttGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAgaga                      >  1:10404884/1‑140 (MQ=255)

                                                                                                                    gCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATg                    >  1:5854336/1‑140 (MQ=255)

                                                                                                                       gATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTg                 >  1:3629231/1‑140 (MQ=255)

                                                                                                                         tataTTTGCATGTCTAGTTGTTTTTCCAGGGAGATGATGATGAGTAAAAAATTGGCCACAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgag               <  1:994562/137‑1 (MQ=255)

                                                                                                                         tAGCTTTGCCTGTCTAGTTGTGTTTACAGGGAGATGATGATGAGTAAAAAATGGGCCAAACAGCGCCAGCCGGGGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgag               <  1:10634708/140‑1 (MQ=255)

                                                                                                                         tAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGTTGATGAGTAAAAAATTGTCCAAAAAGCGCCAGCCTGTGAAGACCGTGGTGGCGAAATATCCTGCTAGCAACGCCAAAAATTTTGGCTATGAAGAGATGTTgag               <  2:6429315/140‑1 (MQ=255)

                                                                                                                         tAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTACAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgag               <  1:6674332/140‑1 (MQ=255)

                                                                                                                         tAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgag               <  1:5056915/140‑1 (MQ=255)

                                                                                                                         tAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgag               <  1:7777890/140‑1 (MQ=255)

                                                                                                                         tAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgag               <  2:2066564/140‑1 (MQ=255)

                                                                                                                         tAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgag               <  1:11115444/140‑1 (MQ=255)

                                                                                                                         tAGATTTGCCTGTCTAGTTGTTTTGACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgag               <  1:7879754/140‑1 (MQ=255)

                                                                                                                         tAGAGTTGCCCGGCTATTTGTTTGTCCAGGGAGATGCTGATGAGTAAAAAATTCGCCAAACAGCGCCAGCCGGTGAAGCCCGTGGTGGCGTAAGAACCTGCTCGCACCGCCAAATATTTTGGCTATGAAGAGATGTTgag               <  2:10895360/140‑1 (MQ=255)

                                                                                                                          aGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgagc              <  1:5226859/140‑1 (MQ=255)

                                                                                                                             tttGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgagcgag           >  1:9003423/1‑140 (MQ=255)

                                                                                                                                  cTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCTGGa      <  2:72985/140‑1 (MQ=255)

                                                                                                                                  cTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCTGGa      <  1:8496399/140‑1 (MQ=255)

                                                                                                                                    gTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCTGGAAg    <  2:8932330/140‑1 (MQ=255)

AAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCTGGAAGCT  >  NZ_CP025268/3255568‑3255841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`