BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,298,720	C→T	G35E (GGA→GAA)	yraQ ←	permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,298,720	0	C	T	99.5%	3131.8 / NA	821	G35E (GGA→GAA)	yraQ	permease
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (345/470); minor base G (2/2); total (349/472)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 833 total aligned reads displayed.

CCGCTTTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTATGATAATTGAG  >  NZ_CP025268/3298583‑3298856

ccgcTTTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTtcc                                                                                                                                        >  1:10188541/1‑140 (MQ=255)

ccgcTTTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTtcc                                                                                                                                        >  1:9286224/1‑140 (MQ=255)

  gcTTTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCa                                                                                                                                      <  1:10370773/140‑1 (MQ=255)

    tttCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCCTGGGTTCGCATCCGCCTGCGCAAGGATAGATTGACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCAtt                                                                                                                                    <  2:5842137/140‑1 (MQ=255)

        cATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTcac                                                                                                                                <  1:8305204/140‑1 (MQ=255)

         aTACCGCGAGGAATTAGATCATCGCGTATTCCAACGCCGTCTGCGATTGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATTCTGTGGGTTTCGGCTGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTgaca                                                                                                                               <  2:6477063/140‑1 (MQ=255)

           aCCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATa                                                                                                                             >  2:1241232/1‑140 (MQ=255)

             cgcgAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATAcc                                                                                                                           >  2:4521738/1‑140 (MQ=255)

             cgcgAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATAcc                                                                                                                           >  2:5570969/1‑140 (MQ=255)

              gcgAGGACATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCa                                                                                                                          >  2:10444010/1‑140 (MQ=255)

              gcgAGGAAATATATCATCGCGTAATCCAAAGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGTATAGATTAACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTACCATTGCACATACCa                                                                                                                          >  2:6889660/1‑140 (MQ=255)

              gcgAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGAGAGATTTACCGATACGGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTCGTAAGGTTCCCATTTCACATACCa                                                                                                                          >  2:8776325/1‑140 (MQ=255)

               cgAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCag                                                                                                                         >  1:10835910/1‑140 (MQ=255)

               cgAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGAGTTACCGATACTGTGGGTTTCGGCAGCAGTAATCGCTTTGCCGTAGTAAGGTTCCCATTTCACATTCCag                                                                                                                         >  1:9731353/1‑140 (MQ=255)

                gAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCaga                                                                                                                        >  1:2539630/1‑140 (MQ=255)

                  ggAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAgaga                                                                                                                      <  1:4949690/140‑1 (MQ=255)

                  cgAAATAGATCATCGCGTAATCCAACGCCGCCAGCCATGGGTTAGCATCCGCCAGCGCAAGGATAGATTTACCGATACTGTNGGTTTCGGGTGCAGTAAAAGGTTTGCCGTAGTAAGGTTCCCATTCCACATACCAgaga                                                                                                                      <  1:5970354/139‑1 (MQ=255)

                           tcatcGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATg                                                                                                             >  2:8954756/1‑140 (MQ=255)

                              tcGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACa                                                                                                          >  2:2697384/1‑140 (MQ=255)

                              tcGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGGAGTAAGGTTCCCATTTCACATACCAGAGCCCGGCACTGACa                                                                                                          >  2:2162881/1‑140 (MQ=255)

                               cgcgTAATCCAACGCCGCCTGCCAAGGGTTAGCATCCGCCTGCTCAAGGATATATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACaa                                                                                                         <  2:9594403/140‑1 (MQ=255)

                                 cgTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACg                                                                                                       <  1:6415716/140‑1 (MQ=255)

                                       ccAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGagaaag                                                                                                 <  1:3866327/140‑1 (MQ=255)

                                         aaCGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGagaaagaa                                                                                               <  1:3998423/140‑1 (MQ=255)

                                         aaCGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGagaaagaa                                                                                               >  1:3686840/1‑140 (MQ=255)

                                           cgccgcCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGaaaa                                                                                             >  1:8133391/1‑140 (MQ=255)

                                              cgccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAgcg                                                                                          <  2:1434685/140‑1 (MQ=255)

                                              cgccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAgcg                                                                                          <  2:8433440/140‑1 (MQ=255)

                                                ccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATCCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTCGTAAGGTTCCCATTTCACATACAATAGACCGGCAATGACAACGAGAACGAAAAGcgcg                                                                                        >  2:7530960/1‑140 (MQ=255)

                                                ccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGcgcg                                                                                        >  1:23798/1‑140 (MQ=255)

                                                 cTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCgg                                                                                       <  2:10922919/140‑1 (MQ=255)

                                                 cTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCgg                                                                                       <  2:5147000/140‑1 (MQ=255)

                                                 cTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCgg                                                                                       <  1:1839092/140‑1 (MQ=255)

                                                 cTGCCATGGGTTAGCATCCGCCTGCGCAAGGAGAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCgg                                                                                       <  2:4629839/140‑1 (MQ=255)

                                                      ctGGGTTAGCATCCGCCTGCGCAAGGATAGAATTACCGATACTGTGGGTTTCGGCAGCAGTAACCGCTTTGCCGTAGTACGGGTCCCATTTCACATCCCAGAGACCGGCAATGACACCGAGAAAGACAAGCGCGGGTTTc                                                                                  <  2:1964905/139‑1 (MQ=255)

                                                      aTGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTc                                                                                  <  1:146146/140‑1 (MQ=255)

                                                         ggTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTccac                                                                               <  2:3393946/140‑1 (MQ=255)

                                                          gTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTccacc                                                                              >  2:1852960/1‑140 (MQ=255)

                                                           ttAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTccacca                                                                             <  2:5398742/140‑1 (MQ=255)

                                                             aGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACt                                                                           >  2:4914358/1‑140 (MQ=255)

                                                              gCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTg                                                                          >  2:5953930/1‑140 (MQ=255)

                                                                   cGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCGGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCTCCACTGAATgg                                                                     >  2:7728255/1‑140 (MQ=255)

                                                                   cGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATgg                                                                     >  2:2454658/1‑140 (MQ=255)

                                                                   cGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATgg                                                                     >  1:5595953/1‑140 (MQ=255)

                                                                    gCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATggg                                                                    >  1:7356569/1‑140 (MQ=255)

                                                                             aGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTgccgc                                                           <  1:7538313/140‑1 (MQ=255)

                                                                               gagaGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCt                                                         <  2:11209132/137‑1 (MQ=255)

                                                                                   gATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTgaga                                                     >  2:6604216/1‑140 (MQ=255)

                                                                                   gATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTgaga                                                     >  1:3334180/1‑140 (MQ=255)

                                                                                        aCCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGTAg                                                >  1:8377478/1‑140 (MQ=255)

                                                                                        aCCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAg                                                >  1:6113421/1‑140 (MQ=255)

                                                                                         ccGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGa                                               <  2:3873296/140‑1 (MQ=255)

                                                                                             tACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGa                                           <  2:2261403/140‑1 (MQ=255)

                                                                                             tACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGa                                           <  1:9060489/140‑1 (MQ=255)

                                                                                              acggtgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACAGACCAGAGACCGGCAATGACAACGAGAAAGAAACGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          <  1:2661303/137‑1 (MQ=255)

                                                                                              aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGACTGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          >  1:9438880/1‑140 (MQ=255)

                                                                                              aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          <  1:11181118/140‑1 (MQ=255)

                                                                                              aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          <  1:2897558/140‑1 (MQ=255)

                                                                                              aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          <  1:10953276/140‑1 (MQ=255)

                                                                                              aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          <  1:1670371/140‑1 (MQ=255)

                                                                                              aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          <  2:2924545/140‑1 (MQ=255)

                                                                                              aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          <  1:6671819/140‑1 (MQ=255)

                                                                                              aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTACACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          <  2:424884/140‑1 (MQ=255)

                                                                                              aCTGTGGGTTGCGGCAGCAGGAAACGCTTTGCCGTAGTAAGGGTCCCCTTTCACATACCAGAGACCGGCTATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGACTGGGCGTTGCCGCCTGAGATGAAGACTGAc                                          <  2:10234784/140‑1 (MQ=255)

                                                                                               cTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAcc                                         <  1:9176124/140‑1 (MQ=255)

                                                                                                  tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGt                                      >  2:6281556/1‑140 (MQ=255)

                                                                                                  tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGt                                      >  1:8629051/1‑140 (MQ=255)

                                                                                                  tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGt                                      >  1:4315749/1‑140 (MQ=255)

                                                                                                  tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGt                                      >  1:411724/1‑140 (MQ=255)

                                                                                                    ggTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCa                                    <  1:2078510/140‑1 (MQ=255)

                                                                                                     gTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCAt                                   >  1:3037361/1‑140 (MQ=255)

                                                                                                       ttCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTca                                                                                                                                 <  1:571825/44‑1 (MQ=255)

                                                                                                       ttCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGACTGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAg                                 >  1:10535961/1‑140 (MQ=255)

                                                                                                       ttCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAg                                 >  2:10026763/1‑140 (MQ=255)

                                                                                                       ttCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAg                                 >  1:6295481/1‑140 (MQ=255)

                                                                                                       ttCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAg                                 >  2:4041243/1‑140 (MQ=255)

                                                                                                         cgtcagcacTAAACGCATTGCCGTAGTAAGGGTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCa                               <  2:6187189/137‑1 (MQ=255)

                                                                                                         cGGCAGCAGTAAACGCTTTGCCTTAGTTAGGTTCCCATTTCACATACCAGAGACCTGCAATGACAACGAGAAAGATAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCa                               <  2:9423519/140‑1 (MQ=255)

                                                                                                         cGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAgcgc                                                                                         <  2:5674340/82‑1 (MQ=255)

                                                                                                         cGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCa                               <  1:1220456/140‑1 (MQ=255)

                                                                                                         cGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCa                               <  2:10760975/140‑1 (MQ=255)

                                                                                                         cGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCa                               <  1:4744389/140‑1 (MQ=255)

                                                                                                            cagcagTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTc                            >  2:3042913/1‑140 (MQ=255)

                                                                                                             agcagTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTcc                           <  1:9892796/140‑1 (MQ=255)

                                                                                                              gcagTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTccc                          <  1:7071726/140‑1 (MQ=255)

                                                                                                               cagTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAAAGAGAAAGACAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTcccc                         <  2:8268121/140‑1 (MQ=255)

                                                                                                               cagTAAACGCTTTGCCGTAGTAAGGGTCCCATTTCACCTACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTcccc                         <  2:9791134/140‑1 (MQ=255)

                                                                                                                agTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCa                        >  1:7920207/1‑140 (MQ=255)

                                                                                                                    aaCGCTTTGCCGTAGTAAGGGTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGaa                    <  2:3176282/140‑1 (MQ=255)

                                                                                                                     aCGCTTTGCCGTAGTAAGGTTCCCATGTCACATACCAGAGACCGGCAATGACAACGAGACAGAAACGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCCGTCATAGCATTCCCCAGGAAt                   <  1:2222308/140‑1 (MQ=255)

                                                                                                                       gCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATaa                 <  2:803789/140‑1 (MQ=255)

                                                                                                                         tttGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGt               >  1:7700845/1‑140 (MQ=255)

                                                                                                                         tttGCCGTAGTAAGGTTACCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGt               <  2:7029720/140‑1 (MQ=255)

                                                                                                                           tGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCAGAGCATTACCCAGGACTAAGGTa             >  2:3568526/1‑140 (MQ=255)

                                                                                                                            gCCGTAGTAAGGTTCGCCTTTCTCATACCAGAGACCGGCAATGTCAAGGAGAAAGTAAAGCGCGGGTTTCCACCCCTGAAGGGGCGTTGCCGCGTGTGATGACGAGTGCCCAGTCCTAGCATTCCCCTGGCTTACGTTAt            >  2:9219482/1‑140 (MQ=255)

                                                                                                                                tagtaAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTATGATa        >  1:5312894/1‑140 (MQ=255)

                                                                                                                                 agtaAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTATGATaa       >  2:1595659/1‑140 (MQ=255)

                                                                                                                                    aaGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTATGATAATTg    <  2:1677111/140‑1 (MQ=255)

                                                                                                                                      ggTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTATGATAATTGAg  <  1:8924492/140‑1 (MQ=255)

                                                                                                                                      ggTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTATGATAATTGAg  <  1:10090323/140‑1 (MQ=255)

CCGCTTTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTATGATAATTGAG  >  NZ_CP025268/3298583‑3298856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`