BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,434,451	A→G	T26A (ACA→GCA)	def →	peptide deformylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,434,451	0	A	G	99.6%	2399.8 / NA	690	T26A (ACA→GCA)	def	peptide deformylase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (295/391); minor base T (3/0); total (298/392)
Fisher's exact test for biased strand distribution p-value = 8.04e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 690 total aligned reads displayed.

GGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATACAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGC  >  NZ_CP025268/3434314‑3434586

ggggATTTGTCTAGAATAGAAGAAAT‑ATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAg                                                                                                                                      >  1:6481049/1‑140 (MQ=255)

  ggATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGa                                                                                                                                     >  1:1683821/1‑140 (MQ=255)

        gTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCa                                                                                                                               >  1:10664233/1‑140 (MQ=255)

             gaatagaaGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTa                                                                                                                          <  1:7134181/140‑1 (MQ=255)

             gaatagaaGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTa                                                                                                                          <  1:4438034/140‑1 (MQ=255)

              aatagaaGAAATANTCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGANGAAGTGAATGCAGAAATTCAGCGTAt                                                                                                                         >  1:5934110/1‑140 (MQ=255)

                tagaaGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTAtcg                                                                                                                       <  1:657903/140‑1 (MQ=255)

                   tagaaCTAATCTTTCTAACTCCTGAACACATCTCTGGAGAGTTATGTCAGTATTGCAAGTGTTACCTATTCCGGTCGTGCGGCGTCGCAACGTTGGTAAACCGGTAGGAGTAGTGAATGCAGACATTCAGCGTAtcgtcg                                                                                                                    >  2:1234537/2‑140 (MQ=255)

                   aagaaATAATCTTTCTAACTCCTGAACACTTCTCTGGAGATTTATTTCAGTTTTGGAAGGGTTACATATTCCGGACGAGCGGCTTCGCACAGGTGCTAAACCGGTAGCAGAAGTGAATGCAGAAATTCAGCGGAtcgtcg                                                                                                                    >  2:8883914/1‑140 (MQ=255)

                   aagaaATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTCGAAGAAGTGAATGCAGAAATTCAGCGTAtcgtcg                                                                                                                    >  1:3414585/1‑140 (MQ=255)

                   aagaaATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTAtcgtcg                                                                                                                    >  1:4025088/1‑140 (MQ=255)

                   aagaaATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTAtcgtcg                                                                                                                    >  2:3507071/1‑140 (MQ=255)

                   aagaaATAATCTTTCTAACTCCGGAACACATCTCTGGAGATTGATGTCAGTTTTGCAAGGGTTACATATGCCGGACGCGCGGCTGCGCAAAGTTGCTTGGCCGGTAGAAGAAGTGAATGCAGAATTTCAGCGTAtcgtcg                                                                                                                    >  2:150668/1‑140 (MQ=255)

                      aaaTAATCTTACTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATAGTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCgatg                                                                                                                 <  2:7389711/140‑1 (MQ=255)

                      aaaTAATCTGTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCgatg                                                                                                                 <  2:9776604/140‑1 (MQ=255)

                        ataatCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCgatgat                                                                                                               <  1:8935416/140‑1 (MQ=255)

                        ataatCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCgatgat                                                                                                               >  2:6613227/1‑140 (MQ=255)

                        ataatCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCgatgat                                                                                                               >  1:3188024/1‑140 (MQ=255)

                        ataatCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCgatgat                                                                                                               <  2:3356488/140‑1 (MQ=255)

                        ataatCTTTCAAACTCCTGAACACATCTCTGGAGATTTATGTCATTTTAGCAACTGTTACATATTACGGACGAGCGGCATCGCAAAGTTGCTAAACAGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATAGTAgaggat                                                                                                               >  2:8633621/1‑140 (MQ=255)

                        ataatCGTTCTCACTCCTGAACACATCTCTGGAGATTTATGTCAGATGTGCAAGTGTGACATAGTCCGGACGAGCGGCGTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCgatgag                                                                                                               <  2:6971884/140‑2 (MQ=255)

                         taatCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGGAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGata                                                                                                              >  1:4731222/1‑140 (MQ=255)

                             cTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGtt                                                                                                          <  1:8488567/140‑1 (MQ=255)

                               ttCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCg                                                                                                        <  1:3470208/140‑1 (MQ=255)

                                tCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCga                                                                                                       <  1:4986623/140‑1 (MQ=255)

                                 cTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTACACCGGTAGAAGAAGTGAATGCCGAAATTCAGCGCATCGTCGATGATATGTTCgag                                                                                                      >  1:5547241/1‑140 (MQ=255)

                                 cTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCgag                                                                                                      <  1:9529053/140‑1 (MQ=255)

                                      tCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGAt                                                                                                 <  1:10011303/140‑1 (MQ=255)

                                      tCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGAt                                                                                                 <  2:3230831/140‑1 (MQ=255)

                                        cTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGt                                                                                               >  1:1971125/1‑140 (MQ=255)

                                           cacacATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACg                                                                                            <  1:8241291/139‑1 (MQ=255)

                                           aaCACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACg                                                                                            <  2:1114387/140‑1 (MQ=255)

                                           aaCACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACg                                                                                            <  2:8070186/140‑1 (MQ=255)

                                           aaCACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACg                                                                                            <  1:1211042/140‑1 (MQ=255)

                                             cacaTCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCa                                                                                          <  2:2894063/140‑1 (MQ=255)

                                             cacaTCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCa                                                                                          <  2:10137121/140‑1 (MQ=255)

                                              acaTCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCag                                                                                         >  2:10852462/1‑140 (MQ=255)

                                                aTCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCagaa                                                                                       >  1:5130782/1‑140 (MQ=255)

                                                  ctctGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATTTTCGAGACGATTTAAGCagaata                                                                                     >  2:9652211/1‑138 (MQ=255)

                                                  ctctGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCagaaga                                                                                     <  1:7460723/140‑1 (MQ=255)

                                                  ctctGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCagaaga                                                                                     >  2:8437903/1‑140 (MQ=255)

                                                          aTTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTg                                                                             >  1:9846703/1‑140 (MQ=255)

                                                            ttATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTCAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGc                                                                           >  2:9234474/1‑140 (MQ=255)

                                                                  cAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTggcg                                                                     <  1:1384515/140‑1 (MQ=255)

                                                                  cAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTggcg                                                                     <  2:2042158/140‑1 (MQ=255)

                                                                  cAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTggcg                                                                     <  2:5316419/140‑1 (MQ=255)

                                                                   aggtttGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTggcgg                                                                    <  1:6277836/137‑1 (MQ=255)

                                                                   aGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTACACCGGTAGATGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTggcgg                                                                    >  2:6349629/1‑140 (MQ=255)

                                                                    tttttGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAATTGAATGCAGAAATTCAGCGTATCGTCTATTATATGTTCGCGACGATTTACGCAGAAGAAGGTATTGGCCTggcggc                                                                   <  2:2577976/139‑1 (MQ=255)

                                                                    gTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTggcggc                                                                   >  1:10240766/1‑140 (MQ=255)

                                                                     ttttGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTAGCTAACCCGGTCGCAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCa                                                                  <  2:8277475/140‑1 (MQ=255)

                                                                       ttGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAAc                                                                <  2:6813432/140‑1 (MQ=255)

                                                                        tGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAAcc                                                               <  1:10796924/140‑1 (MQ=255)

                                                                        tGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAAcc                                                               <  1:5419197/140‑1 (MQ=255)

                                                                        tGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAAcc                                                               <  1:2429462/140‑1 (MQ=255)

                                                                             gtgtTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGt                                                          <  1:7946527/140‑1 (MQ=255)

                                                                             gtgtTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGt                                                          >  1:4184561/1‑140 (MQ=255)

                                                                               gtTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTg                                                        <  2:3962495/140‑1 (MQ=255)

                                                                               gtTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTg                                                        >  2:5068279/1‑140 (MQ=255)

                                                                                 tACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGTAACCCAGGTTGat                                                      <  1:9717624/140‑1 (MQ=255)

                                                                                 tACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGat                                                      <  2:10532231/140‑1 (MQ=255)

                                                                                   cATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCCGGTTGatat                                                    >  1:299325/1‑140 (MQ=255)

                                                                                   cATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGatat                                                    <  1:6763451/140‑1 (MQ=255)

                                                                                       ttCCGGACGAGCGGCTTCGCAAAGGTGCTAAACCGGAAGAAGAAGAGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCAt                                                <  2:9071230/140‑1 (MQ=255)

                                                                                       gtCCGGACGAGCGGCTTCGCAAAGTTGCTACACCGGTAGAAGAAGTGAATGCAGACATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCAt                                                <  2:616442/139‑1 (MQ=255)

                                                                                        tCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATc                                               >  1:9366741/1‑140 (MQ=255)

                                                                                        tCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATc                                               >  1:8086339/1‑140 (MQ=255)

                                                                                         ccGGACGAGCGGCTTCGCAAAGTTGCTACACCGGTAGAAGAAGTGAATGCAGCAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCa                                              <  2:11282968/140‑1 (MQ=255)

                                                                                          cGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCaa                                             >  1:380861/1‑140 (MQ=255)

                                                                                          cGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCaa                                             <  2:4361842/140‑1 (MQ=255)

                                                                                                  cGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCAt                                     >  1:7830176/1‑140 (MQ=255)

                                                                                                  cGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCAt                                     >  2:4544977/1‑140 (MQ=255)

                                                                                                  cGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCAt                                     <  2:4163381/140‑1 (MQ=255)

                                                                                                   ggCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCAtt                                    <  1:10117166/140‑1 (MQ=255)

                                                                                                               ttGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGtt                        <  2:7665486/140‑1 (MQ=255)

                                                                                                               ttGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGtt                        >  2:4791106/1‑140 (MQ=255)

                                                                                                               ttGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGtt                        >  1:11072850/1‑140 (MQ=255)

                                                                                                               ttGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGtt                        >  1:6988996/1‑140 (MQ=255)

                                                                                                                  cTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAATAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCg                     >  2:2733591/1‑140 (MQ=255)

                                                                                                                  cTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCg                     >  2:212795/1‑140 (MQ=255)

                                                                                                                    aaaCCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGa                   <  1:2567167/140‑1 (MQ=255)

                                                                                                                       ccGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGaaaa                <  2:7208919/140‑1 (MQ=255)

                                                                                                                         ggTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGTTGTTATGTTCGAGACGATGTAAGCAGAAGTAGGTATTGGCCTGGCGGCAACCCAGGTTTATATCCATCAACGTATCATTGTTATTGATGTTTCGTAAAAcc              >  2:3813227/1‑140 (MQ=255)

                                                                                                                         ggTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAAcc              >  2:5723119/1‑140 (MQ=255)

                                                                                                                         ggTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGAGGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAAcc              >  1:1485534/1‑140 (MQ=255)

                                                                                                                               agaagTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACg        <  2:3266664/140‑1 (MQ=255)

                                                                                                                               agaagTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACg        <  1:11278873/140‑1 (MQ=255)

                                                                                                                                gaagTGACTGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGa       <  1:6128586/140‑1 (MQ=255)

                                                                                                                                gaagTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCGGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGa       <  1:2735524/140‑1 (MQ=255)

                                                                                                                                 aagTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGaa      >  2:2407219/1‑140 (MQ=255)

                                                                                                                                  agTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAAc     >  1:10472070/1‑140 (MQ=255)

                                                                                                                                  agTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAAc     <  2:5548545/140‑1 (MQ=255)

                                                                                                                                  agTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAAc     >  1:8626079/1‑140 (MQ=255)

                                                                                                                                  agTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAAc     >  2:1694989/1‑140 (MQ=255)

                                                                                                                                  agTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCAATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAAc     >  2:10223821/1‑140 (MQ=255)

                                                                                                                                   gagAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTAAGCAGAAGAAGGTATTTGCCTGGCGGCAACCCAGGTTGATATACATCAACGTATCATTGTTATTGATGTTTCGGAAAAACGTGACGaaag    >  1:7645649/3‑138 (MQ=255)

                                                                                                                                     gAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGc  <  1:2117254/140‑1 (MQ=255)

                                                                                                                                     gAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGc  <  1:8723857/140‑1 (MQ=255)

                                                                                                                                     gAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGc  >  2:9470920/1‑140 (MQ=255)

                                                                                                                                     caaTGCAGAAATACCGCGTATCGTCGAAGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGTTATCCATCAACGTATCATTGTTATTGAAGTTTCGGAAAACCGTGACGAACGGc  <  2:10711886/139‑1 (MQ=255)

GGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATACAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGC  >  NZ_CP025268/3434314‑3434586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`