Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,483,653 C→T P560L (CCG→CTG)  yheS → ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,483,6530CT99.2% 2287.1 / ‑5.9 603P560L (CCG→CTG) yheSABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (358/237);  minor base G (0/5);  total (359/244)
Fisher's exact test for biased strand distribution p-value = 1.04e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 618 total aligned reads displayed.

TATCAACAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGCCTGCCTGCAACAG  >  NZ_CP025268/3483517‑3483789
                                                                                                                                        |                                                                                                                                        
tATCAACAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACtgcg                                                                                                                                       >  1:10008439/1‑140 (MQ=255)
tATCAACAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACtgcg                                                                                                                                       >  2:7196675/1‑140 (MQ=255)
  tCAACAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTa                                                                                                                                     >  1:3096623/1‑140 (MQ=255)
  tCAACAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTa                                                                                                                                     >  1:937608/1‑140 (MQ=255)
  tCAACAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTa                                                                                                                                     >  2:11197976/1‑140 (MQ=255)
    aaCAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTaaa                                                                                                                                   >  2:4259461/1‑140 (MQ=255)
       aGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAagag                                                                                                                                >  1:7249824/1‑140 (MQ=255)
        gTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAgaga                                                                                                                               >  1:11041746/1‑140 (MQ=255)
         tGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGAt                                                                                                                              >  1:9090558/1‑140 (MQ=255)
         tGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGAGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGGACAGAGAt                                                                                                                              >  2:10770980/1‑140 (MQ=255)
          ggTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGACGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGAtt                                                                                                                             >  1:8178190/1‑140 (MQ=255)
           gTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTg                                                                                                                            >  2:2372002/1‑140 (MQ=255)
                   aCGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTg                                                                                                                    >  2:9677205/1‑140 (MQ=255)
                   aCGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTg                                                                                                                    >  1:9526720/1‑140 (MQ=255)
                      tACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAATGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGaa                                                                                                                 >  2:5812397/1‑140 (MQ=255)
                              cAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGAt                                                                                                         >  2:4426339/1‑140 (MQ=255)
                              cAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGAt                                                                                                         >  1:1068279/1‑140 (MQ=255)
                                ggAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAATGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATgg                                                                                                       >  1:7400368/1‑140 (MQ=255)
                                ggAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATgg                                                                                                       >  1:4677588/1‑140 (MQ=255)
                                        aGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCCGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCCGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTg                                                                                               <  2:4565103/140‑1 (MQ=255)
                                            cgacgaAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAAcg                                                                                           <  2:9405605/140‑1 (MQ=255)
                                              acgaAGCGCCATGAGAGAATGCGAACTGCGCCCGGGCACGTAAAGATCAGAAGCGTCGGGAAGCGGAGCTGCGTGCGGAATCCGGGCCACTGCGTAGAGTGTTTGCCCGTGTGGATAATGAGGGGGAGGAGCGGAAcgcg                                                                                         >  2:914775/1‑140 (MQ=255)
                                              acgaAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAAcgcg                                                                                         >  2:3194599/1‑140 (MQ=255)
                                              acgaAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAAcgcg                                                                                         >  1:9024720/1‑140 (MQ=255)
                                              acgaAGCGACAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAAcgcg                                                                                         >  2:6532464/1‑140 (MQ=255)
                                                    cgcCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTg                                                                                   >  2:5274731/1‑140 (MQ=255)
                                                       cAAAAGAGAATGCGAACCGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCACACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGgcg                                                                                <  2:6394199/140‑1 (MQ=255)
                                                        aaaaGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGgcgc                                                                               <  1:1888179/140‑1 (MQ=255)
                                                         aaaGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCa                                                                              >  1:10756518/1‑140 (MQ=255)
                                                         aaaGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCa                                                                              >  1:5260688/1‑140 (MQ=255)
                                                         aaaGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCa                                                                              >  1:2365721/1‑140 (MQ=255)
                                                               aaTGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGACAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGa                                                                        >  2:6009245/1‑140 (MQ=255)
                                                                 tGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAag                                                                      <  2:2064143/140‑1 (MQ=255)
                                                                 tGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGAGCAAACCCAGACACAGCGTAAAGAGATTGCCCGTCTGGAAAATGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAag                                                                      >  1:6148906/1‑140 (MQ=255)
                                                                     aaCAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGc                                                                           >  2:3826593/1‑131 (MQ=255)
                                                                      aCAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGaaa                                                                 >  2:4151339/1‑140 (MQ=255)
                                                                         gcgcCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGc                                                                           >  1:4142436/1‑127 (MQ=255)
                                                                          cgcCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGACAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCg                                                             >  2:1625545/1‑140 (MQ=255)
                                                                           gcCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCgg                                                            <  1:9783925/140‑1 (MQ=255)
                                                                            cccAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGACAAAGAGATGGAGACGCTGAACGCGCAACTGGCGCAGGCGGACGAGACACTCGGc                                                           >  2:10057085/1‑140 (MQ=255)
                                                                            cccAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAATGAGATGGAGACGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGc                                                           >  2:8896437/1‑140 (MQ=255)
                                                                             ccAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCg                                                          <  2:1512468/140‑1 (MQ=255)
                                                                               aGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGAc                                                        >  1:9279348/1‑140 (MQ=255)
                                                                                 gCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAg                                                      <  1:2796202/140‑1 (MQ=255)
                                                                                   ccGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAACAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCg                                                    <  1:4894633/139‑1 (MQ=255)
                                                                                   aCGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCg                                                    <  1:1780353/140‑1 (MQ=255)
                                                                                    cGTAACGATCCGAAGCGACCGGCCGCAGCGCAGCGGGCGCAAACCCCGCCACGGCGTAAAGAGAGCGCCCGTCTGGCCAAAGACATGGAGCAGCTGACCGCCCAACGGGCGCAGGCGGAAGAGAACCTCGGCGACAGCGa                                                   <  2:2761288/140‑1 (MQ=255)
                                                                                    cGTAAAGCTCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                   <  1:8695200/140‑1 (MQ=255)
                                                                                    cGTAAAGATCCGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGACGAGAACCTCGGCGACAGCGa                                                   >  2:6937789/1‑140 (MQ=255)
                                                                                    cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                   <  1:1456800/140‑1 (MQ=255)
                                                                                    cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                   >  2:1961006/1‑140 (MQ=255)
                                                                                    cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                   >  1:11224215/1‑140 (MQ=255)
                                                                                    cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                   >  1:3444687/1‑140 (MQ=255)
                                                                                    cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                   <  2:7470410/140‑1 (MQ=255)
                                                                                    cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                   >  1:5772215/1‑140 (MQ=255)
                                                                                    cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                   <  2:8644738/140‑1 (MQ=255)
                                                                                    cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                   <  2:10982704/140‑1 (MQ=255)
                                                                                    cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                   >  1:10489406/1‑140 (MQ=255)
                                                                                    cGTAAAGAACAGACGCGACGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                   <  1:6594550/140‑1 (MQ=255)
                                                                                     gTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGaa                                                  <  1:1528910/140‑1 (MQ=255)
                                                                                      tAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAAc                                                 >  2:8460584/1‑140 (MQ=255)
                                                                                      tAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAAc                                                 <  1:2051775/140‑1 (MQ=255)
                                                                                         aGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGt                                              <  2:2597187/140‑1 (MQ=255)
                                                                                         aGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGt                                              <  2:3047128/140‑1 (MQ=255)
                                                                                         aGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGt                                              <  2:4870135/140‑1 (MQ=255)
                                                                                         aGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGt                                              <  1:3928794/140‑1 (MQ=255)
                                                                                           aTCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAATGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGACGTGAAACTCGGCGACAGCGAACTGTAt                                            >  2:10417120/1‑140 (MQ=255)
                                                                                           aTCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTAt                                            >  2:5640934/1‑140 (MQ=255)
                                                                                           aTCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTAt                                            >  1:10221147/1‑140 (MQ=255)
                                                                                           aTCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTAt                                            >  1:5166937/1‑140 (MQ=255)
                                                                                              aGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGAc                                         <  1:10844664/140‑1 (MQ=255)
                                                                                              aGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGAc                                         <  1:7653712/140‑1 (MQ=255)
                                                                                               gAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGAcc                                        >  1:2142658/1‑140 (MQ=255)
                                                                                               gAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGAcc                                        >  1:3016565/1‑140 (MQ=255)
                                                                                               gAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGAcc                                        >  1:8034817/1‑140 (MQ=255)
                                                                                               gAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGAcc                                        >  1:6975020/1‑140 (MQ=255)
                                                                                                   cGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAATGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCagag                                    >  2:9585465/1‑140 (MQ=255)
                                                                                                     tCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGcc                                  >  1:8834848/1‑140 (MQ=255)
                                                                                                     tCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGcc                                  >  1:6458908/1‑140 (MQ=255)
                                                                                                     tCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGcc                                  >  1:365080/1‑140 (MQ=255)
                                                                                                      ctggAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCg                                 <  2:677339/138‑1 (MQ=255)
                                                                                                       gggAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGt                                >  1:8514329/1‑140 (MQ=255)
                                                                                                        ggAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTa                               >  1:2635145/1‑140 (MQ=255)
                                                                                                          aaGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTaaa                             >  2:825838/1‑140 (MQ=255)
                                                                                                                agctgCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAg                       >  1:4063198/1‑140 (MQ=255)
                                                                                                                       tgcgCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCg                >  2:130245/1‑140 (MQ=255)
                                                                                                                       tgcgCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCg                >  2:147842/1‑140 (MQ=255)
                                                                                                                        gcgcAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCgc               <  2:4053141/140‑1 (MQ=255)
                                                                                                                        gcgcAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCgc               <  1:437602/140‑1 (MQ=255)
                                                                                                                        gcgcAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCgc               <  2:2899812/140‑1 (MQ=255)
                                                                                                                        gcgcAAACCCAGCCACGGCGTAAAGCGCTTGCCCCTCTGGAAAAACAGCAGGATAAGCTGACCGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGACCTGTATGACCAGAGCCGTAAAGCGGAGTTGACCgc               <  1:7554648/140‑1 (MQ=255)
                                                                                                                           cAAACCCAGCCACTGCGGAAAGAGATTGCCCGTCGGGTAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACGGTCTGACCAGAGCCGTAAAGCGGAGTTGACCgcctg            >  2:7677947/1‑140 (MQ=255)
                                                                                                                               cccAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGcctgcctg        >  2:798703/1‑140 (MQ=255)
                                                                                                                               cccAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGcctgcctg        <  1:6053535/140‑1 (MQ=255)
                                                                                                                               cccAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGcctgcctg        >  1:5475149/1‑140 (MQ=255)
                                                                                                                                ccAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGCctgcctgc       <  1:3251543/140‑1 (MQ=255)
                                                                                                                                ccAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGCctgcctgc       <  1:1210669/140‑1 (MQ=255)
                                                                                                                                 cAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGCCTGCCTGCa      >  1:6765188/1‑140 (MQ=255)
                                                                                                                                   gCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGCCTGCCTGCAAc    >  1:7853723/1‑140 (MQ=255)
                                                                                                                                   gCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGCCTGCCTGCAAc    >  1:5610154/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                        
TATCAACAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGCCTGCCTGCAACAG  >  NZ_CP025268/3483517‑3483789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑