BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,504,638	A→G	262 (TAA→TAG)	frlD →	fructoselysine 6‑kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,504,638	0	A	G	98.8%	1668.5 / NA	483	262 (TAA→TAG)	frlD	fructoselysine 6‑kinase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (208/269); minor base T (0/6); total (208/275)
Fisher's exact test for biased strand distribution p-value = 3.94e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 508 total aligned reads displayed.

TCGACACCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAACTCCTCT  >  NZ_CP025268/3504502‑3504774

tCGACACCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTggtaggta                                                                                                                                       <  1:10128355/140‑1 (MQ=255)

tCGACACCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTggtaggta                                                                                                                                       <  1:775642/140‑1 (MQ=255)

tCGACACCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTggtaggta                                                                                                                                       <  2:5789963/140‑1 (MQ=255)

tCGACACCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTggtaggta                                                                                                                                       >  1:9209227/1‑140 (MQ=255)

tCGACACCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTggtaggta                                                                                                                                       <  1:9507670/140‑1 (MQ=255)

         tGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTgg                                                                                                                              >  2:3419275/1‑140 (MQ=255)

         tGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTgg                                                                                                                              >  2:1096485/1‑140 (MQ=255)

          gggTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGc                                                                                                                             <  1:1436332/140‑1 (MQ=255)

           ggTGCCGGAGAGTCGTTCATGGCCGGATTCCGTTGCGGCTGGTCTGCGGGGCTGACAGTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATACCGTTGGCg                                                                                                                            <  2:6238349/140‑1 (MQ=255)

            gTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGt                                                                                                                           >  1:7080647/1‑140 (MQ=255)

            gTGCCGGAGATTCGTTCATTGCCGGATTCCTTAGCGGCTGGTCAGCGGGGATGACATTACCGCAGTCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGt                                                                                                                           >  2:5704983/1‑140 (MQ=255)

             tGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTg                                                                                                                          <  2:4872826/140‑1 (MQ=255)

               ccGGAGATTCGTGCATTGCCGGAGTCCTGTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGACGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAg                                                                                                                        <  2:5917417/140‑1 (MQ=255)

                cGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGc                                                                                                                       >  1:8114073/1‑140 (MQ=255)

                     aTTCGATCAGTGCCGGAATCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCtt                                                                                                                  <  1:6469034/140‑1 (MQ=255)

                       tCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCa                                                                                                                >  1:7969530/1‑140 (MQ=255)

                       tCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCa                                                                                                                >  2:2860446/1‑140 (MQ=255)

                          ttCATGGCCGGATTCCATTGCGGCTGGTCTGCGGGGATGACATTCCCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTGGGCGTGAGCATCTTCACGc                                                                                                             <  1:7450253/140‑1 (MQ=255)

                            cATTGCCGGATTCCTTTGCGGCTGGTCTGCGGTGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCa                                                                                                           <  2:10716317/140‑1 (MQ=255)

                            cATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCa                                                                                                           <  1:3374073/140‑1 (MQ=255)

                               tGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACg                                                                                                        <  1:7839067/140‑1 (MQ=255)

                                 ccGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTg                                                                                                      <  2:2267562/140‑1 (MQ=255)

                                    gATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGGATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTg                                                                                                   >  2:1528329/1‑140 (MQ=255)

                                           ttGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTg                                                                                            >  1:2077032/1‑140 (MQ=255)

                                              cGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCg                                                                                         >  2:2956205/1‑140 (MQ=255)

                                                     tCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCCACGTGCTGTTACTTGCCGGAAAACg                                                                                  <  1:732699/140‑1 (MQ=255)

                                                     tCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACg                                                                                  <  2:7017374/140‑1 (MQ=255)

                                                     tCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACg                                                                                  <  2:723056/140‑1 (MQ=255)

                                                     tCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACg                                                                                  <  2:2096150/140‑1 (MQ=255)

                                                      cTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGa                                                                                 <  1:11147528/140‑1 (MQ=255)

                                                      cTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGa                                                                                 <  2:6036070/140‑1 (MQ=255)

                                                         cGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTCTAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGAccc                                                                              >  1:10011275/1‑140 (MQ=255)

                                                              aTGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCAGTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATaa                                                                         >  1:2973098/1‑140 (MQ=255)

                                                               tGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCCCGGTGCCTGGTAGGTATACCGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAAt                                                                        <  1:8327456/140‑1 (MQ=255)

                                                                   aTTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGa                                                                    >  1:6152036/1‑140 (MQ=255)

                                                                    ttACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGTTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAg                                                                   <  2:7455619/140‑1 (MQ=255)

                                                                      aCCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTa                                                                 >  2:3070178/1‑140 (MQ=255)

                                                                      aCCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTa                                                                 >  2:8420814/1‑140 (MQ=255)

                                                                          cAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCAATCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTGACTTGCCGGACAACGACCCTATACTCCGAGTCattc                                                             >  2:8840132/1‑140 (MQ=255)

                                                                            ggCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattcat                                                           >  2:4437554/1‑140 (MQ=255)

                                                                            ggCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattcat                                                           >  2:4344713/1‑140 (MQ=255)

                                                                            gccGATAGCGCACGGACCGGCGTGAGCGCCCAAAACCATTCACTCCCACGGTGCCTGGTAGGAATAACGTTGGCGTGCGCATCTTCACGCCAACGTGCTGTTACTGGCCGGCACACGAGCCTAAACTCCGAGTaaggcat                                                           >  2:8244873/3‑135 (MQ=255)

                                                                             gCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattcatt                                                          >  1:5876372/1‑140 (MQ=255)

                                                                             gCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattcatt                                                          >  1:2904114/1‑140 (MQ=255)

                                                                               gATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCt                                                        >  1:10357411/1‑140 (MQ=255)

                                                                               gATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCt                                                        >  2:1795397/1‑140 (MQ=255)

                                                                                aTAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCtt                                                       <  1:10474530/140‑1 (MQ=255)

                                                                                    cgcAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCtt                                                                                                                  <  1:4921124/77‑1 (MQ=255)

                                                                                    cgcAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCaa                                                                                                          >  1:183821/1‑85 (MQ=255)

                                                                                         ggAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAgg                                              >  2:1242291/1‑140 (MQ=255)

                                                                                         ggAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAg                                               <  2:6699272/139‑1 (MQ=255)

                                                                                               gCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAAGGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAACACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGAt                                        <  1:1499378/140‑1 (MQ=255)

                                                                                                cGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGAtt                                       >  1:315466/1‑140 (MQ=255)

                                                                                                cGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGAtt                                       <  2:9114923/140‑1 (MQ=255)

                                                                                                 gTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATACTCCGAGTAATTCATTCTTTATTTCAGGGTCGATTa                                      >  1:7188622/1‑140 (MQ=255)

                                                                                                  tGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTAt                                     <  1:5720546/140‑1 (MQ=255)

                                                                                                    cgcgGCGAAAACCATTCAGTACCTCGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGt                                   <  1:4458024/140‑1 (MQ=255)

                                                                                                    cgcgGCGAAAACCATTCAGTACCACGGTGCCTGGTAGTTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAAGTCATTCTTTATTTCAGGGTCGATTATGt                                   <  2:3729862/140‑1 (MQ=255)

                                                                                                    cgcgGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGt                                   <  2:10141842/140‑1 (MQ=255)

                                                                                                    cgcgGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGt                                   <  2:1603740/140‑1 (MQ=255)

                                                                                                    cgcgGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTGGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGt                                   <  2:9491451/140‑1 (MQ=255)

                                                                                                         cgAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCt                              <  2:11287240/140‑1 (MQ=255)

                                                                                                          gAAAACCATTCAGTACCCCGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTa                             <  1:6923743/140‑1 (MQ=255)

                                                                                                            aaaCCATTCCGTACCCCGGTGCCTGGTAGGTAAAAAGTTGGCGTGAGCATCTTCACGCAAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg                           <  1:8524609/140‑1 (MQ=255)

                                                                                                            aaaCCATTCAGTACCCCGGTGCCTGGTAGTTATAACTTTGGCGTGAGCATCTTCACGCAAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg                           <  1:5155218/140‑1 (MQ=255)

                                                                                                            aaaCCATTCAGTACCCCGGTGCCTGGTAGGTATACCGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg                           <  1:11331104/140‑1 (MQ=255)

                                                                                                            aaaCCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCACCGTGCTGTTCCTTGCCGGAAAACGACCCTATACTCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg                           <  2:4159799/140‑1 (MQ=255)

                                                                                                            aaaCCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg                           <  1:3694042/140‑1 (MQ=255)

                                                                                                            aaaCCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg                           <  2:5059504/140‑1 (MQ=255)

                                                                                                            aaaCCATACAGTACCACGGGGCCTGGTAGTTATACCGTTGGCGTGAGCTTCTTCACGCCAACGTGCTGTTAGTTGCCTTAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg                           <  1:2729192/140‑1 (MQ=255)

                                                                                                              aCCATTCAGTACCACGGTGCCTGGTAGGTATACCGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGa                         <  1:4693607/140‑1 (MQ=255)

                                                                                                              aCCATTCAGTAACACGGTTCCTGGTATGTATAACGTTGGCGTGAGCTTCTTCACGCCAAGGTGCTGTTACTTGCCGGAAAAAGACACTATATTCCGAGTAATTCATTATTTATTTCAGGTTCGATTATGTCAGCTTCGGa                         <  2:9713854/140‑1 (MQ=255)

                                                                                                               ccATTCAGTCCCACGGTGCCTGGTAGGTATAACGTGGGCGTGAGCATCTTCCCGCCAACGTGCTTTTACTGGCCGGAACACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGAc                        <  1:8719737/140‑1 (MQ=255)

                                                                                                                    ccgTACCACGGTGCCTGGTAGGTATAACGTGGGCGTGCGCATCTTCACGCCAACGTGCTGTTACGTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTa                   <  2:1424628/138‑1 (MQ=255)

                                                                                                                    cAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTCCACGCCAACGTGCTGTTACTGGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTa                   <  1:9078765/140‑1 (MQ=255)

                                                                                                                    cAGGACCACGGTGCCTGGTAGGGATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTa                   <  2:6885849/140‑1 (MQ=255)

                                                                                                                      ggaCCCCGGTGCCTGGTAGGGATCACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACAGCTAct                 <  2:5643615/138‑1 (MQ=255)

                                                                                                                      gTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTAct                 <  2:4551641/140‑1 (MQ=255)

                                                                                                                         ccACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACtctc              <  2:7862408/140‑1 (MQ=255)

                                                                                                                          cACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTACTTCATTCTTTATTTCAGGGTCGAGTATGTCAGCTACGGACCGCTACTCtca             >  2:9340353/1‑140 (MQ=255)

                                                                                                                           aCGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCtcat            >  1:2875999/1‑140 (MQ=255)

                                                                                                                            cGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCtcatc           >  1:10998080/1‑140 (MQ=255)

                                                                                                                            cGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCGACTCtcatc           >  2:8679472/1‑140 (MQ=255)

                                                                                                                              gTGCCTGGTAGGTATACCGTTGGCGTGAGCATATTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCaa         <  2:7619371/140‑1 (MQ=255)

                                                                                                                              gTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCaa         <  2:3280144/140‑1 (MQ=255)

                                                                                                                              gTGCCTGGTAGGAATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCaa         <  1:6689456/140‑1 (MQ=255)

                                                                                                                              gTGCCGGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCaa         <  1:4146662/140‑1 (MQ=255)

                                                                                                                               tGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAAc        <  1:6299281/140‑1 (MQ=255)

                                                                                                                               tGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAAGTCCTTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAAc        >  1:9810095/1‑140 (MQ=255)

                                                                                                                                 ccTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGTCCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGAGTATGTCAGCTACGGACCGCTACTCTCATCAActc      >  2:5438183/1‑140 (MQ=255)

                                                                                                                                 ccTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg                           <  2:10493032/119‑1 (MQ=255)

                                                                                                                                 ccTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAActc      >  1:5334945/1‑140 (MQ=255)

                                                                                                                                 ccTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAActc      >  1:2188479/1‑140 (MQ=255)

                                                                                                                                 ccTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAActc      >  1:3994642/1‑140 (MQ=255)

                                                                                                                                 ccTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAActc      <  2:315466/140‑1 (MQ=255)

                                                                                                                                 ccTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAActc      >  2:2603447/1‑140 (MQ=255)

                                                                                                                                 ccTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAAGTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAActc      >  2:8064480/1‑140 (MQ=255)

                                                                                                                                   tGGTAGGTATATCGTTGGCGTGATCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAActcct    <  1:10891227/140‑1 (MQ=255)

                                                                                                                                   tGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAActcct    <  2:1895569/140‑1 (MQ=255)

                                                                                                                                     gTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAACTCctct  >  1:2382535/1‑140 (MQ=255)

TCGACACCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAACTCCTCT  >  NZ_CP025268/3504502‑3504774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`