BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,743,921	T→C	I172I (ATT→ATC)	galK →	galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,743,921	0	T	C	99.8%	2133.1 / NA	609	I172I (ATT→ATC)	galK	galactokinase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (290/318); minor base A (1/0); total (291/318)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 609 total aligned reads displayed.

TAAGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATTATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTACC  >  NZ_CP025268/3743785‑3744056

tAAGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATg                                                                                                                                      >  1:9666873/1‑140 (MQ=255)

tAAGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGAAGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTCACTGCGGGATCATg                                                                                                                                      >  1:5205698/1‑140 (MQ=255)

  aGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGa                                                                                                                                    >  1:4890121/1‑140 (MQ=255)

  aGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGa                                                                                                                                    >  2:769644/1‑140 (MQ=255)

   gTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGAt                                                                                                                                   <  1:7153487/140‑1 (MQ=255)

   gTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGAt                                                                                                                                   <  2:10068094/140‑1 (MQ=255)

   gTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGAt                                                                                                                                   <  2:2840735/140‑1 (MQ=255)

   gTTCTTCCGCGTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGAt                                                                                                                                   <  2:8382908/140‑1 (MQ=255)

       ttcCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGc                                                                                                                               >  2:9671485/1‑140 (MQ=255)

        tcCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCt                                                                                                                              >  2:10518564/1‑140 (MQ=255)

        tcCGCGGCACGGGCAGTCGCGGTCGGCACCGCATTGCAGCAGCTTTCTCAGCGGCCGCGGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCCGTTTGTCGGCTGTAACTGCGGGATCATGGATCAGCt                                                                                                                              <  2:6745878/140‑1 (MQ=255)

             ttCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAAttt                                                                                                                         >  2:4363311/1‑140 (MQ=255)

              tCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTc                                                                                                                        >  1:2772102/1‑140 (MQ=255)

                    gAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCt                                                                                                                  <  2:4125310/140‑1 (MQ=255)

                      aGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCg                                                                                                                >  2:6554674/1‑140 (MQ=255)

                       gTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCCTCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCgg                                                                                                               <  2:3033605/140‑1 (MQ=255)

                         cgcgGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCa                                                                                                             >  1:5816107/1‑140 (MQ=255)

                           cgGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCaag                                                                                                           <  2:185347/140‑1 (MQ=255)

                            ggTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCaaga                                                                                                          <  1:9758009/140‑1 (MQ=255)

                            ggTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCaaga                                                                                                          <  2:1023766/140‑1 (MQ=255)

                            ggTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGGAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCaaga                                                                                                          <  2:9161307/140‑1 (MQ=255)

                             gTCGGAACCGTATTGCAGCAGCTTTCTCATCTGCCGCTGGACGGCGCACAAATCGCGCTTACCGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCaagaa                                                                                                         <  2:9360564/140‑1 (MQ=255)

                                ggAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCaagaaaga                                                                                                      <  1:2229365/140‑1 (MQ=255)

                                ggAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCaagaaaga                                                                                                      >  1:8603640/1‑140 (MQ=255)

                                  aaCCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATc                                                                                                    >  1:4552255/1‑140 (MQ=255)

                                       tATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGcc                                                                                               <  1:10224591/140‑1 (MQ=255)

                                        aTTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCt                                                                                              >  2:3288051/1‑140 (MQ=255)

                                        aTTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCt                                                                                              >  1:6406678/1‑140 (MQ=255)

                                          tGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTg                                                                                            >  2:884512/1‑140 (MQ=255)

                                          tGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTg                                                                                            >  1:8407660/1‑140 (MQ=255)

                                            cagcagCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCt                                                                                          >  2:7206333/1‑140 (MQ=255)

                                            cagcagCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCt                                                                                          >  1:7528728/1‑140 (MQ=255)

                                             agcagcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTg                                                                                         >  2:1923266/1‑140 (MQ=255)

                                              gcagcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGa                                                                                        <  1:6852023/140‑1 (MQ=255)

                                              gcagcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGa                                                                                        <  1:4068001/140‑1 (MQ=255)

                                               cagcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGAt                                                                                       >  2:6187941/1‑140 (MQ=255)

                                               cagcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGAt                                                                                       >  2:2335580/1‑140 (MQ=255)

                                               cagcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGAt                                                                                       >  1:2919737/1‑140 (MQ=255)

                                               cagcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGAt                                                                                       >  2:6877289/1‑140 (MQ=255)

                                                 gcTGTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGGAGGCTGTAACTGCGGGATCATGGCTGAGCTAAGGTGCGCGCGCGGCACGAAGGATGATGCGTTGCTgttgg                                                                                     >  2:7072221/1‑136 (MQ=255)

                                                  cTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGa                                                                                    >  1:1800530/1‑140 (MQ=255)

                                                  cTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGa                                                                                    <  2:10713380/140‑1 (MQ=255)

                                                     tATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTg                                                                                 <  2:5754027/140‑1 (MQ=255)

                                                     tATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTg                                                                                 <  1:765292/140‑1 (MQ=255)

                                                       tcatcTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGGAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGcc                                                                               <  2:858087/140‑1 (MQ=255)

                                                          tcTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCt                                                                            <  1:5467909/140‑1 (MQ=255)

                                                          tcTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCt                                                                            >  2:4809688/1‑140 (MQ=255)

                                                          tcTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCt                                                                            <  1:2068917/140‑1 (MQ=255)

                                                            tGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCa                                                                          >  2:347507/1‑140 (MQ=255)

                                                            tGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCa                                                                          >  1:7340058/1‑140 (MQ=255)

                                                              ccGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACt                                                                        >  1:1046593/1‑140 (MQ=255)

                                                                        gcgcACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCaaa                                                              >  1:3294964/1‑140 (MQ=255)

                                                                         cgcACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAg                                                             >  2:9562735/1‑140 (MQ=255)

                                                                         cgcACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAg                                                             >  1:3465144/1‑140 (MQ=255)

                                                                                 tCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTcc                                                     <  2:6383875/140‑1 (MQ=255)

                                                                                 tCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTcc                                                     <  1:10685570/140‑1 (MQ=255)

                                                                                 tCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTcc                                                     <  1:7941853/140‑1 (MQ=255)

                                                                                 tCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTcc                                                     <  2:8265539/140‑1 (MQ=255)

                                                                                 tCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACGGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTcc                                                     <  1:6719957/140‑1 (MQ=255)

                                                                                    cgcTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATg                                                  >  2:2644761/1‑140 (MQ=255)

                                                                                       ttAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGccc                                               <  1:9003259/140‑1 (MQ=255)

                                                                                        tAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCa                                              >  2:8057543/1‑140 (MQ=255)

                                                                                         aaCGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCaa                                             <  2:11279017/140‑1 (MQ=255)

                                                                                         aaCGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGGTTCCATGCCCaa                                             <  2:7499346/140‑1 (MQ=255)

                                                                                          aCGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCaaa                                            >  2:1385500/1‑140 (MQ=255)

                                                                                                 ggAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTgg                                     <  2:592271/140‑1 (MQ=255)

                                                                                                    aGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTg                                  <  1:4313357/140‑1 (MQ=255)

                                                                                                    aGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTg                                  <  2:10858898/140‑1 (MQ=255)

                                                                                                    aGCAGAAAACCAGTTTGTAGGCTGTAACAGCGGGAACATGGCACAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCAGGCCCAAAGGTGTGGCTg                                  <  1:10057895/140‑1 (MQ=255)

                                                                                                       aGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcg                               <  2:2553157/140‑1 (MQ=255)

                                                                                                       aGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcg                               <  2:3728147/140‑1 (MQ=255)

                                                                                                       aGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcg                               <  2:1479304/140‑1 (MQ=255)

                                                                                                       aGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcg                               <  1:9305300/140‑1 (MQ=255)

                                                                                                       aGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcg                               <  2:6957352/140‑1 (MQ=255)

                                                                                                       aGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcg                               <  1:11120412/140‑1 (MQ=255)

                                                                                                       aGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcg                               <  2:11037401/140‑1 (MQ=255)

                                                                                                         aaaaCCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTACTTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcgtc                             <  2:5313147/140‑1 (MQ=255)

                                                                                                            aCCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAGTTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGGAGTTTCCATGCCCAAAGGTGTGGCTGTCGtcatc                          >  1:8240599/1‑140 (MQ=255)

                                                                                                              cAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGtcatcat                        <  1:4141641/140‑1 (MQ=255)

                                                                                                                 tttGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGTCTGTCGTCATCATCaa                     >  2:1734643/1‑140 (MQ=255)

                                                                                                                     tAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCTAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGt                 <  2:735050/140‑1 (MQ=255)

                                                                                                                        gCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAAc              <  1:9439461/140‑1 (MQ=255)

                                                                                                                          tGTATCTGCGGGATCCTGGATCAGCTAATTTCCGCGCTCTGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGTCCATTGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACtt            <  1:995093/140‑1 (MQ=255)

                                                                                                                          tGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACtt            <  1:3653773/140‑1 (MQ=255)

                                                                                                                           gTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTc           >  1:6556118/1‑140 (MQ=255)

                                                                                                                           gTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTc           >  1:3901416/1‑140 (MQ=255)

                                                                                                                            tAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCa          >  1:118397/1‑140 (MQ=255)

                                                                                                                            tAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCa          >  2:4963546/1‑140 (MQ=255)

                                                                                                                             aaCTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCaa         <  2:7848776/140‑1 (MQ=255)

                                                                                                                               cTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAAc       >  1:8848491/1‑140 (MQ=255)

                                                                                                                               cTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAAc       >  2:5073020/1‑140 (MQ=255)

                                                                                                                                tGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACg      <  1:6077593/140‑1 (MQ=255)

                                                                                                                                  cGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTa    >  1:5872109/1‑140 (MQ=255)

                                                                                                                                  cGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTa    >  1:1406353/1‑140 (MQ=255)

                                                                                                                                    ggATCATGGATCAGCTCATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTAcc  >  2:5525703/1‑140 (MQ=255)

                                                                                                                                    ggATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTAcc  <  1:192643/140‑1 (MQ=255)

                                                                                                                                    ggATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTAcc  <  2:2025021/140‑1 (MQ=255)

                                                                                                                                    ggATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTAcc  <  1:2549145/140‑1 (MQ=255)

                                                                                                                                    ggATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTAcc  >  1:1680164/1‑140 (MQ=255)

                                                                                                                                    ggATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTAcc  <  1:2390889/140‑1 (MQ=255)

TAAGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATTATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTACC  >  NZ_CP025268/3743785‑3744056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`