Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,941,138 A→G *331* (TAA→TAG) 
F465S (TTT→TCT) 		 rbsR →
mdtD ←		 ribose operon transcriptional repressor RbsR
multidrug transporter subunit MdtD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,941,1380AG99.6% 2386.1 / NA 683*331* (TAA→TAG) 
F465S (TTT→TCT) 		rbsR
mdtD		ribose operon transcriptional repressor RbsR
multidrug transporter subunit MdtD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (313/366);  minor base T (1/2);  total (314/369)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 683 total aligned reads displayed.

TTAACCACTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCAT  >  NZ_CP025268/3941001‑3941274
                                                                                                                                         |                                                                                                                                        
  aaCCACTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGAttt                                                                                                                                      <  2:7006316/140‑1 (MQ=255)
     cACTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTTATGTACTCATCCATCGGATAACCCAGCCGTCCCTTCAGCAACAACGATTACAACTTACTCCGAGTCTGATGGAACGCGGTTCGGCTTAGATTTACg                                                                                                                                   <  2:8257923/140‑1 (MQ=255)
     cACTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACg                                                                                                                                   <  2:5219560/140‑1 (MQ=255)
     cACTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACg                                                                                                                                   <  1:9272817/140‑1 (MQ=255)
     cACTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACg                                                                                                                                   >  2:1749066/1‑140 (MQ=255)
      aCTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGc                                                                                                                                  <  2:2404849/140‑1 (MQ=255)
       cTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCt                                                                                                                                 >  1:10707608/1‑140 (MQ=255)
        tATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATAGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTAAGATTTACGCTg                                                                                                                                >  2:4707708/1‑140 (MQ=255)
             accaACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCttt                                                                                                                           >  2:3494212/1‑140 (MQ=255)
                 aCCGAACGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGAt                                                                                                                       <  1:11177920/140‑1 (MQ=255)
                 aCCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGAt                                                                                                                       >  2:9050986/1‑140 (MQ=255)
                  ccGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATc                                                                                                                      >  2:4772466/1‑140 (MQ=255)
                  ccGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATc                                                                                                                      <  1:3473788/140‑1 (MQ=255)
                  ccGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATc                                                                                                                      >  2:3243180/1‑140 (MQ=255)
                    gAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCaa                                                                                                                    >  1:8527423/1‑140 (MQ=255)
                           gAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAAttatta                                                                                                             >  1:3938695/1‑140 (MQ=255)
                            aaCTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTAc                                                                                                            >  2:3899985/1‑140 (MQ=255)
                               tGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCAt                                                                                                         <  1:9680230/140‑1 (MQ=255)
                               tGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCAt                                                                                                         <  1:10909078/140‑1 (MQ=255)
                                 ggggAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCg                                                                                                       >  1:10021106/1‑140 (MQ=255)
                                  gggAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCgg                                                                                                      <  2:53753/140‑1 (MQ=255)
                                  gggAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCgg                                                                                                      <  1:8701900/140‑1 (MQ=255)
                                  gggAGCTGGCGATTGATGTACTCAACCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCgg                                                                                                      <  1:9186461/140‑1 (MQ=255)
                                   ggAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGAGTTACGCTGTCTTTTGATCAAATTATTACCATCGgt                                                                                                     >  1:11315510/1‑140 (MQ=255)
                                      gCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGgttgt                                                                                                  >  2:11223848/1‑140 (MQ=255)
                                       cTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGgttgtt                                                                                                 <  1:684852/140‑1 (MQ=255)
                                           cGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                             <  2:1991820/140‑1 (MQ=255)
                                           cGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                             <  1:6667992/140‑1 (MQ=255)
                                           cGATTGAGGTACTCCTCCAGCGGATAACCCAGCCGACCCTTCTGCAACAACGTTTACAACTTACTCCGAGTCTGATGGACCGCGGGTCGGCGGAGGTTTACGCTGTCTGTTGATCATAGTATTACCTTCGGTTGTTTTcg                                                                                             >  2:3101658/1‑139 (MQ=255)
                                               tGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAg                                                                                         >  1:2218708/1‑140 (MQ=255)
                                                gaggTACTCTTCCATCGGTTTTCCAAGCCGACACGTCAGCAACTTCGATTACAACTTACTCCGATTCTGATGGATCGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTTGTACGATCGGTTGTTTACTGAAGc                                                                                        <  1:6136947/137‑1 (MQ=255)
                                                gATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGc                                                                                        <  2:4924642/140‑1 (MQ=255)
                                                    tACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGa                                                                                    >  1:8805908/1‑140 (MQ=255)
                                                    tACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGa                                                                                    >  1:7682890/1‑140 (MQ=255)
                                                     aCTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGaa                                                                                   <  2:6375054/140‑1 (MQ=255)
                                                     aCTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGaa                                                                                   <  2:2857168/140‑1 (MQ=255)
                                                      cTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGAATGAAc                                                                                  >  1:10367290/1‑140 (MQ=255)
                                                      cTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTGTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGatc                                                                                  >  2:10238488/1‑138 (MQ=255)
                                                        catccatcGGCGAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGCTGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACAt                                                                                <  1:1959484/140‑1 (MQ=255)
                                                         atccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACAtt                                                                               <  1:823271/140‑1 (MQ=255)
                                                         atccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACAtt                                                                               >  1:9412354/1‑140 (MQ=255)
                                                           ccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtgc                                                                             >  1:6335999/1‑140 (MQ=255)
                                                           ccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGGTGTTTTCAGAAGCATGAACATtgc                                                                             >  2:6023542/1‑140 (MQ=255)
                                                              tcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtgctgc                                                                          >  1:1500211/1‑140 (MQ=255)
                                                              tcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtgctgc                                                                          >  2:4400131/1‑140 (MQ=255)
                                                                ggATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGAt                                                                                                                       >  1:3318931/1‑93 (MQ=255)
                                                                ggATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGctgctg                                                                        >  2:72098/1‑140 (MQ=255)
                                                                    aaCCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                                    <  1:4805528/140‑1 (MQ=255)
                                                                    aaCCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                                    <  2:1244841/140‑1 (MQ=255)
                                                                    aaCCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                                    <  1:7888445/140‑1 (MQ=255)
                                                                    aaCCCAGCCGACCCTTCAGCAACAACGAGTACAACTTACTCCGATTCTGATTGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                                    <  2:10593275/140‑1 (MQ=255)
                                                                     aCCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCa                                                                   >  2:7938635/1‑140 (MQ=255)
                                                                      cccAGCCGACCCTTCAGCAACAACGCTTACAACTTACTCCGATTCTGATGTAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCaa                                                                  <  1:6418174/140‑1 (MQ=255)
                                                                      cccAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCaa                                                                  >  2:11077539/1‑140 (MQ=255)
                                                                      cccAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCaa                                                                  <  2:5605366/140‑1 (MQ=255)
                                                                       ccAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCCACGGATGTTTTCAGACGCATGAACATGGCTGCTGAAGCAAc                                                                 <  2:6786851/140‑1 (MQ=255)
                                                                       ccAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAAc                                                                 <  2:5846555/140‑1 (MQ=255)
                                                                         aGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAg                                                               >  2:1551711/1‑140 (MQ=255)
                                                                         aGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAg                                                               <  2:363189/140‑1 (MQ=255)
                                                                         aGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAg                                                               <  1:3726806/140‑1 (MQ=255)
                                                                           ccGACCCTTCAGCAACAACGATTACCACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGATGGTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGta                                                             >  2:4245953/1‑140 (MQ=255)
                                                                           ccGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTGTTCAGAAGCAGGAACATTGCTGCGGAAGCAACAGta                                                             >  2:4535290/1‑140 (MQ=255)
                                                                             gACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGtaat                                                           <  1:2759040/140‑1 (MQ=255)
                                                                               cccTTCAGCAACAACGATTACAACTGACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCGTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGtaatat                                                         >  2:9332062/1‑139 (MQ=255)
                                                                                 cTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATg                                                       >  2:9661583/1‑140 (MQ=255)
                                                                                 cTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATg                                                       >  2:10366093/1‑140 (MQ=255)
                                                                                     aGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCa                                                   <  1:4370558/140‑1 (MQ=255)
                                                                                      gCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCAt                                                  <  1:7419355/140‑1 (MQ=255)
                                                                                       caacaaCGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCAtt                                                 <  1:9122692/140‑1 (MQ=255)
                                                                                       caacaaCGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGATTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCAtt                                                 <  2:2477193/140‑1 (MQ=255)
                                                                                           aacGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGa                                             <  1:5646995/140‑1 (MQ=255)
                                                                                           aacGATTAAAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTATATTAACGCTGTCTTTAGCTCAAATTATTACCATCGGTTGTATTCAGAAGCATGAACAATGCTGCTGAAGCAACAGTAATAATGCCCATTGTGa                                             <  2:9477023/140‑1 (MQ=255)
                                                                                            acGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGATGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCAGTGTGAt                                            >  2:8353992/1‑140 (MQ=255)
                                                                                             cGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATa                                           >  1:585080/1‑140 (MQ=255)
                                                                                                 tACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACg                                       <  1:10259122/140‑1 (MQ=255)
                                                                                                 tACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACg                                       >  1:2443857/1‑140 (MQ=255)
                                                                                                 tACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACg                                       <  2:7416022/140‑1 (MQ=255)
                                                                                                 tACAACTTACTCCGATTCTGATGGAACGCGGTACGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTAGTTTTCAGAAGCATGAACATTGCTGCTGAAGAAACAGTAATAATGCCCATTGTGATaaaag                                       <  2:7593437/140‑3 (MQ=255)
                                                                                                   catcTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGta                                     <  1:6572942/137‑1 (MQ=255)
                                                                                                   cAACTTACTCCGCTTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTAATGATCAAATTATTACCATCGTTTGTTTTCAGAAGCATGAACATTGCTGCTGAATCAACAGTAAAAATGACCATTGTGATAAACGta                                     <  1:5359573/140‑1 (MQ=255)
                                                                                                   cAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGta                                     <  1:5466934/140‑1 (MQ=255)
                                                                                                   cAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGta                                     <  1:3061417/140‑1 (MQ=255)
                                                                                                   cAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGta                                     <  1:4572940/140‑1 (MQ=255)
                                                                                                   cAACTTACTCCGATTCGGATGCAACGCGGATCTGCTTAGCTTTACGCTGACTTTTCATAAAATTATTACCATCTGTTGTTTTCAGAAGCATGAACATAGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGta                                     <  2:2767845/140‑1 (MQ=255)
                                                                                                               aTTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGt                         >  1:7198589/1‑140 (MQ=255)
                                                                                                               aTTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGt                         >  1:1722826/1‑140 (MQ=255)
                                                                                                               aTTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCAGTGTGATAAACGTATAGTGGAATTGt                         >  2:8664136/1‑140 (MQ=255)
                                                                                                                ttCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGtt                        >  2:6242958/1‑140 (MQ=255)
                                                                                                                ttCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGtt                        >  2:2268520/1‑140 (MQ=255)
                                                                                                                    gctGGAACGGGGTTCGGCTTAGATTTCCGCGGTCATGAGATCCCATAATTCCCATCGCGTGTATTCAGCAGCATGAACCGTGCTGCTGAAGCACCAGACATAATGCCCATTGTGAGAAACGTATAGTGGAATTGCTCGAc                    <  2:10882595/138‑1 (MQ=255)
                                                                                                                      ggtaccgcgGTGCGGCTTAGATTTTCTCTGTCTATAGATCAAATTATCACCCTCGGTTGTTTTCAGAAGCAAGAACAGGGCTGATGAAGCACCAGTAATAATGCCCATATTGATAAACGTATAGTGGAATTGTTCGACAg                  <  2:6575708/135‑1 (MQ=255)
                                                                                                                         aaCGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCg               >  2:9194675/1‑140 (MQ=255)
                                                                                                                         aaCGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGAAAGTCg               >  2:10017841/1‑140 (MQ=255)
                                                                                                                           cgcgGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGtt             >  1:1264402/1‑140 (MQ=255)
                                                                                                                            gcgGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATGACCATCGGTTGTTTTCAGAAGCATGAACAGTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTtg            <  1:9795318/140‑1 (MQ=255)
                                                                                                                              ggTTCGGCTTAGATTTACGCTGTCTTTTGCTCAAATTATGACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTtgtg          <  2:4996600/140‑1 (MQ=255)
                                                                                                                              ggTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTtgtg          <  1:8000229/140‑1 (MQ=255)
                                                                                                                              ggTTCGGCTTAGATTTACGATGTCTTTTGATCAAATTATTACCATCGTTTGTTTTCAGAAGCATGAACATTGCTTCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTtgtg          <  2:2650684/140‑1 (MQ=255)
                                                                                                                               gTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCAGGAACATTGCTGCTGAAGCAACGGTAATAATGCCCATTGTGATCAACGTGTAGTGGAATTGTTCGAGAGTCGTTGGGc         >  2:7309209/1‑140 (MQ=255)
                                                                                                                                      ttAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCAAGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCAt  <  2:8953330/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
TTAACCACTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCAT  >  NZ_CP025268/3941001‑3941274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑