Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 37,904 T→C N405D (AAC→GAC)  caiB ← L‑carnitine CoA‑transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP02526837,9040TC99.7% 2098.0 / NA 606N405D (AAC→GAC) caiBL‑carnitine CoA‑transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (358/244);  minor base A (1/1);  total (361/245)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 630 total aligned reads displayed.

TAAACGTCCGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGATTTGTCCGGGGTTATTTTTGAAT  >  NZ_CP025268/37767‑38040
                                                                                                                                         |                                                                                                                                        
tAAACGTCCGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTcct                                                                                                                                        <  1:3385016/140‑1 (MQ=255)
tAAACGTCCGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTcct                                                                                                                                        <  2:5168103/140‑1 (MQ=255)
tAAACGTCCGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTcct                                                                                                                                        >  2:2229752/1‑140 (MQ=255)
tAAACGTCCGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTcct                                                                                                                                        <  2:571577/140‑1 (MQ=255)
tAAACGTCCGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCAGTTTTGTTCGGTTGTGATTGGGCGGGCGCTAGTCAGGCAAGCCGACGGGCGCCACGCGGTTAGTcct                                                                                                                                        >  1:7380252/1‑140 (MQ=255)
  aaagTCCGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTGTAGTCCTCa                                                                                                                                      >  1:3631072/4‑140 (MQ=255)
  aaCGTCCGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCa                                                                                                                                      >  1:515000/1‑140 (MQ=255)
     gTCCGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACt                                                                                                                                   <  1:3884108/140‑1 (MQ=255)
     gTCCGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACt                                                                                                                                   <  2:11278798/140‑1 (MQ=255)
        cGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTg                                                                                                                                >  1:3115180/1‑140 (MQ=255)
        cGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCGCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGGTTAGTCCTCAACTTTg                                                                                                                                >  1:2589015/1‑140 (MQ=255)
          cAAGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGc                                                                                                                              <  1:9016665/140‑1 (MQ=255)
               tCGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGAc                                                                                                                         <  1:7120255/140‑1 (MQ=255)
                  tCCCACATTTGACGTAGTTGTTGTCCGCCCATGTTATCCATTGCACCTCTATCCATTTTTGTTCGGTTGGTATTGGGCGGGCGCTAGTCAGGCTAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCtt                                                                                                                      >  2:5449619/1‑140 (MQ=255)
                  tCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCtt                                                                                                                      >  2:7991027/1‑140 (MQ=255)
                  tCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCtt                                                                                                                      >  2:3067479/1‑140 (MQ=255)
                  tCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGGTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGGTTAGTCCTCAACTTTGGCCAGACCtt                                                                                                                      >  2:7770901/1‑140 (MQ=255)
                    ccACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTg                                                                                                                    <  1:6496924/140‑1 (MQ=255)
                         tttGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCA‑TTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGAcc                                                                                                              <  2:8702406/140‑1 (MQ=255)
                         atagACGAAGATGTTGTCCGCCAATGAGATCCCTTGCACCTCTATCCATTTTTGTTCGTTTGTTATTCGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGATTAGTCCTCAACTTTGGCCAGACCATTGCTGAc                                                                                                               <  2:9780348/137‑1 (MQ=255)
                          ttGCCGTAGATGTTGTCCGCCACGGATATCCATTGCACCTCTATCCATGTTTGTTCGATTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCACCTTTGGCCAGACCTTGGCTGAcc                                                                                                              <  2:3390185/140‑1 (MQ=255)
                          ttGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGAcc                                                                                                              <  1:10023923/140‑1 (MQ=255)
                          tggACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATGGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGAcc                                                                                                              <  2:7408527/138‑1 (MQ=255)
                            gACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCaa                                                                                                            <  1:1212222/140‑1 (MQ=255)
                             aCGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAAc                                                                                                           >  2:1988210/1‑140 (MQ=255)
                             aCGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAAc                                                                                                           >  1:8605404/1‑140 (MQ=255)
                                  gtggttgtCCGCCAATTATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCAAGTCAGGCAAGCCGACTGACGCCACGCGTGTAGTCCTCAACTTGGGCCAGACCTTTGCTGACAAACTCCTg                                                                                                      >  2:8258488/4‑140 (MQ=255)
                                  gATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTg                                                                                                      >  2:7660269/1‑140 (MQ=255)
                                   aTGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGa                                                                                                     >  2:1783062/1‑140 (MQ=255)
                                   aTGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGa                                                                                                     <  1:1552900/140‑1 (MQ=255)
                                       tgtCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGt                                                                                                 >  1:6393624/1‑140 (MQ=255)
                                        gtCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTc                                                                                                >  2:8842114/1‑140 (MQ=255)
                                        gtCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTc                                                                                                >  1:6856569/1‑140 (MQ=255)
                                         tCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCg                                                                                               >  1:993792/1‑140 (MQ=255)
                                           cGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGtt                                                                                             >  1:11167643/1‑140 (MQ=255)
                                           cGCCAATGATATCCATTGCACCTCTATCCATTATTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCAAGACCTTTGCTGACCAACTCCTGAATGTCggt                                                                                             >  2:10612720/1‑138 (MQ=255)
                                             ccAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGtttt                                                                                           >  2:4628755/1‑140 (MQ=255)
                                                 tGATATCCATTGCACCGCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCt                                                                                       <  2:10764465/140‑1 (MQ=255)
                                                 tGATATCCAGTGCACCTCTCTCCAATTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGATTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGTATGTCGTTTTCGCt                                                                                       <  2:6729459/140‑1 (MQ=255)
                                                     atCCATTGCACCTCTATCCATTTTGGTTCGTTTGTTATTGGGCGGGCGCGAGTCAGGCAATCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAg                                                                                   >  2:4413877/1‑140 (MQ=255)
                                                        cATTGAACCTCTATCCATTTTTGTTCGTTTGTTATTGGGAGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTACTCAACTTTGGACAGACATTTGCTGACCAACTCCTGAATGTCGTTATCGCTTTAGCCg                                                                                >  2:6523364/1‑140 (MQ=255)
                                                          ttGCACCTCTATCCATTTTTGTTCGTGTGTTATTGGGAGGGCTCTAGTAAGGCACTCCTACTGACGCCACGCTTGTAGTCCTCTACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTATACGat                                                                              >  2:4098695/1‑140 (MQ=255)
                                                           tGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGata                                                                             >  2:10956232/1‑140 (MQ=255)
                                                           tGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGata                                                                             <  1:1690281/140‑1 (MQ=255)
                                                           tGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGata                                                                             >  2:10478748/1‑140 (MQ=255)
                                                           tGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCGGTAGCCGata                                                                             >  2:4842432/1‑140 (MQ=255)
                                                           tGCACCTCTATCCATGTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGata                                                                             >  1:10975891/1‑140 (MQ=255)
                                                           tGCACCTCTATCCATGTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGata                                                                             >  1:9542651/1‑140 (MQ=255)
                                                            gCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGatat                                                                            >  1:9388272/1‑140 (MQ=255)
                                                             cACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATAtt                                                                           >  1:7237617/1‑140 (MQ=255)
                                                             cACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATAtt                                                                           >  1:7757514/1‑140 (MQ=255)
                                                                  ctctCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCa                                                                      >  2:9369099/4‑140 (MQ=255)
                                                                  ctATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCa                                                                      >  2:5279964/1‑140 (MQ=255)
                                                                  ctATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCa                                                                      >  1:623815/1‑140 (MQ=255)
                                                                   tATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCaa                                                                     <  1:8395476/140‑1 (MQ=255)
                                                                   gaTCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAAGTTTGGCCAGACCTTTGCTGACCAACTCCTGTATGACGTTTTCGCTGTAGCCGATATTTTTCaa                                                                     <  1:9303839/139‑1 (MQ=255)
                                                                     tCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCaaaa                                                                   >  2:10082119/1‑140 (MQ=255)
                                                                     tCCAGTTGTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCTATATTTTTCaaaa                                                                   >  2:6107646/1‑140 (MQ=255)
                                                                       cATTTTTGTTCGTTTGTTATTGGGAGGGCGCTAGTCAGGCAAGCCGACTGACGCCACTCGATTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTATCCGATATTATTCAAAATg                                                                 >  1:10153812/1‑140 (MQ=255)
                                                                            ttGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGc                                                            >  1:1878185/1‑140 (MQ=255)
                                                                            ttGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGc                                                            >  1:759235/1‑140 (MQ=255)
                                                                             tGTTCTTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGcc                                                           <  2:684066/140‑1 (MQ=255)
                                                                             tGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGcc                                                           <  2:2657092/140‑1 (MQ=255)
                                                                             tGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGcc                                                           >  2:10218704/1‑140 (MQ=255)
                                                                             tGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGcc                                                           <  2:9040388/140‑1 (MQ=255)
                                                                             tGTACGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTGAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGcc                                                           <  1:4384973/140‑1 (MQ=255)
                                                                              gTTCGTTTGTTCTTGGGCGGGCGCTAGACAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCg                                                          <  1:5501774/140‑1 (MQ=255)
                                                                                  gTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTc                                                      <  2:25320/140‑1 (MQ=255)
                                                                                  gTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTc                                                      <  2:3901325/140‑1 (MQ=255)
                                                                                  gTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTGTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTc                                                      <  2:1463236/140‑1 (MQ=255)
                                                                                      gTTATTGGGCGGGCGCTAGTCAGGCAATCCGACTGACGCCACTCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTccatg                                                  <  1:4049747/140‑1 (MQ=255)
                                                                                      gTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTccatg                                                  <  1:4923015/140‑1 (MQ=255)
                                                                                      gTTATAGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTccatg                                                  <  1:10422164/140‑1 (MQ=255)
                                                                                         aTTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTccatgcca                                               <  1:4766901/140‑1 (MQ=255)
                                                                                           tGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTccatgccatg                                             <  1:2305679/140‑1 (MQ=255)
                                                                                                 ggcgCTAGTCAGGCAAGCCGACTGCCGCCACGCGTTTAGTCCTCATCTTTGGCCAGACCTTTGCTGACCAACTCCTGACTGTCGTTTTCGCTGTAGCCGAGATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAggg                                       >  2:1221890/1‑140 (MQ=255)
                                                                                                 ggcgCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCATCTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAggg                                       >  1:2144433/1‑140 (MQ=255)
                                                                                                 ggcgCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCaa                                                                                                            >  1:814240/1‑71 (MQ=255)
                                                                                                 ggcgCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCaa                                                                     <  2:9600926/110‑1 (MQ=255)
                                                                                                 ggcgCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAggg                                       >  1:9136176/1‑140 (MQ=255)
                                                                                                 ggcgCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAggg                                       >  1:29594/1‑140 (MQ=255)
                                                                                                 ggcgCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGACGATATTTTTCAAAATTGCAGCCGTGTCCATGCCATGTGAggg                                       >  2:6260690/1‑140 (MQ=255)
                                                                                                 ggcgCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACCTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAggg                                       >  1:566774/1‑140 (MQ=255)
                                                                                                 ggcgCTAGACAGGCAAGCCGACTGACGCCACGCGATTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCACGCCATGTGAggg                                       <  1:10624618/140‑1 (MQ=255)
                                                                                                     cTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCAtt                                   <  2:8896150/140‑1 (MQ=255)
                                                                                                            ggCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGcc                            >  2:2841870/1‑140 (MQ=255)
                                                                                                             gCAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCa                           >  1:6556512/1‑140 (MQ=255)
                                                                                                              cAAGCCGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAg                          >  1:8788075/1‑140 (MQ=255)
                                                                                                                  ccGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCCACTCCTGCATGTCGTTGTCGCTGTAGCCGATATTTTTCACAATGGCTGCCGTGTCCATGCAATGTGAGGGCATTCCGCGCCAGAgtt                      >  2:5999554/1‑140 (MQ=255)
                                                                                                                  ccGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGAtgt                      >  2:713540/1‑138 (MQ=255)
                                                                                                                  ccGACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGACGCCGAGATTTTTCAAAATGGAAGCCGTGTCCATGCCATGTGAGGGCAGTCCGCGCCAGCttt                      >  2:8526992/1‑140 (MQ=255)
                                                                                                                    gACTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGATTTGt                    <  1:4525906/140‑1 (MQ=255)
                                                                                                                     aCTGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGATTTGTc                   <  2:7310742/140‑1 (MQ=255)
                                                                                                                       tGACGCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGATTTGTCCg                 <  1:9730838/140‑1 (MQ=255)
                                                                                                                           gCCACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGATTTGTCCGGGGt             <  1:5367653/140‑1 (MQ=255)
                                                                                                                           gCCACGCGTTTAGTCCTCAACTTTGGCCAGAACTTTGCTGAACAACTACTGAATGTCGTTTTCGATGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGATGGCATTCCGCGCCAGAATTGTCCGGGGt             <  1:2498385/140‑1 (MQ=255)
                                                                                                                           gCCACGCGTTTAGTCCTCAACTTGGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGGGTCCATGCCATGTGAGGGCATTCCGCGCCAGATTTGTCCGGGGt             <  2:9246385/140‑1 (MQ=255)
                                                                                                                             cACGCGTTTAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGATTTGTCCGGGGTTa           >  1:821264/1‑140 (MQ=255)
                                                                                                                                    ttAGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGAGATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGATTTGTCCGGGGTTATTTTTGa    >  1:6084418/1‑140 (MQ=255)
                                                                                                                                      aGTCCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGATTTGTCCGGGGTTATTTTTGAAt  >  2:3729703/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
TAAACGTCCGCAAGATCGTCCCACATTTGACGTAGATGTTGTCCGCCAATGATATCCATTGCACCTCTATCCATTTTTGTTCGTTTGTTATTGGGCGGGCGCTAGTCAGGCAAGCCGACTGACGCCACGCGTTTAGTTCTCAACTTTGGCCAGACCTTTGCTGACCAACTCCTGAATGTCGTTTTCGCTGTAGCCGATATTTTTCAAAATGGCAGCCGTGTCCATGCCATGTGAGGGCATTCCGCGCCAGATTTGTCCGGGGTTATTTTTGAAT  >  NZ_CP025268/37767‑38040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑