BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,149,401	A→G	114 (TAA→TAG) I280T (ATT→ACT)	frwD → yijO ←	PTS fructose‑like transporter subunit IIB AraC family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,149,401	0	A	G	99.8%	2081.0 / NA	595	114 (TAA→TAG) I280T (ATT→ACT)	frwD yijO	PTS fructose‑like transporter subunit IIB AraC family transcriptional regulator
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (345/249); minor base T (1/0); total (346/249)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 600 total aligned reads displayed.

GCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAATCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCA  >  NZ_CP025268/4149264‑4149536

  cGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCgg                                                                                                                                     >  1:11164921/1‑140 (MQ=255)

           gCGATTTGCACATTGCCGCTATGTGCAATGCAGCTTCTACGCATTCCTGCGTGAGCCGCAGCGGGGAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCTAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGt                                                                                                                            <  2:6263055/140‑1 (MQ=255)

               tttGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCTAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTGCTGTCAGt                                                                                                                        >  2:3089498/1‑140 (MQ=255)

               tttGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCCTCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGt                                                                                                                        >  2:6168372/1‑140 (MQ=255)

                  gAACAGTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTgg                                                                                                                     <  2:8553100/140‑1 (MQ=255)

                   aaCATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGc                                                                                                                    <  2:9258505/140‑1 (MQ=255)

                        tGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTgg                                                                                                               >  1:3907200/1‑140 (MQ=255)

                        tGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTgg                                                                                                               <  2:6526621/140‑1 (MQ=255)

                         gCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGt                                                                                                              >  1:7848828/1‑140 (MQ=255)

                          ccGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTa                                                                                                             >  2:3323975/1‑140 (MQ=255)

                           cGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTAc                                                                                                            <  2:4673284/140‑1 (MQ=255)

                              tATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGc                                                                                                         <  1:4255781/140‑1 (MQ=255)

                                  tgCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGc                                                                                                     <  2:3735427/140‑1 (MQ=255)

                                  tgCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGc                                                                                                     >  1:6057125/1‑140 (MQ=255)

                                  tgCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCGTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGc                                                                                                     >  1:2969502/1‑140 (MQ=255)

                                    cAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGa                                                                                                   >  1:9808120/1‑140 (MQ=255)

                                       tGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGatat                                                                                                >  2:2267544/1‑140 (MQ=255)

                                          agcaTCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTcc                                                                                             >  1:2320323/1‑140 (MQ=255)

                                          agcaTCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTcc                                                                                             >  1:8924143/1‑140 (MQ=255)

                                          agcaTCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTcc                                                                                             >  1:8286088/1‑140 (MQ=255)

                                          agcaTCTACGCATTCCTGCGTGAGCCGCAGAGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGTTACTGCCGGCGATATTcc                                                                                             >  2:11268400/1‑140 (MQ=255)

                                            caTCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCCAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTCTTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATCTTCCGa                                                                                           >  2:8940373/1‑140 (MQ=255)

                                            caTCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGa                                                                                           >  2:281151/1‑140 (MQ=255)

                                            caTCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGa                                                                                           >  2:489836/1‑140 (MQ=255)

                                            caTCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGa                                                                                           >  1:9101385/1‑140 (MQ=255)

                                            caTCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGa                                                                                           >  2:7191996/1‑140 (MQ=255)

                                              tCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACg                                                                                         >  1:5148659/1‑140 (MQ=255)

                                                 aCGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCg                                                                                      >  2:5511972/1‑140 (MQ=255)

                                                 aCGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCg                                                                                      <  2:9159024/140‑1 (MQ=255)

                                                 aCGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCg                                                                                      <  2:3489819/140‑1 (MQ=255)

                                                       tCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGtt                                                                                >  1:6519362/1‑140 (MQ=255)

                                                        ccTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTc                                                                               >  1:4900553/1‑140 (MQ=255)

                                                        ccTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTc                                                                               >  1:8149423/1‑140 (MQ=255)

                                                        ccTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTc                                                                               <  1:10547480/140‑1 (MQ=255)

                                                        ccTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTGGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTc                                                                               <  1:9341676/140‑1 (MQ=255)

                                                         cTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCg                                                                              >  2:831786/1‑140 (MQ=255)

                                                         cTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCg                                                                              >  2:7111928/1‑140 (MQ=255)

                                                          tGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCgg                                                                             >  2:5138555/1‑140 (MQ=255)

                                                            cGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGgtg                                                                           >  1:8705259/1‑140 (MQ=255)

                                                             gTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGgtgt                                                                          <  2:6731375/140‑1 (MQ=255)

                                                             gTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGgtgt                                                                          <  1:8640059/140‑1 (MQ=255)

                                                                aGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGtttt                                                                       >  2:4425003/1‑140 (MQ=255)

                                                                 gCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAATCCCATCTTAGTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGGGGTACTGCCGGCGGTCTTCCGACGGCGAGCGTTCGGTGtttgt                                                                      >  1:1479695/1‑138 (MQ=255)

                                                                 gCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGttttt                                                                      >  2:11163708/1‑140 (MQ=255)

                                                                 gCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGttttt                                                                      >  1:10008276/1‑140 (MQ=255)

                                                                 gCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGttttt                                                                      >  1:8228630/1‑140 (MQ=255)

                                                                 gCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGttttt                                                                      >  2:242795/1‑140 (MQ=255)

                                                                 gCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCGTATTCTGGAGTAGTCTGTTTTTCTGTCAGTTGGCTGTGGTACTTACGGCGATATTCCGACTGCGAGCGTTCGGTGttttt                                                                      >  2:11028472/1‑140 (MQ=255)

                                                                   cGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTAc                                                                    >  1:2637782/1‑140 (MQ=255)

                                                                         gggTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACa                                                              <  1:5499490/140‑1 (MQ=255)

                                                                           gTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGa                                                            <  1:1332666/140‑1 (MQ=255)

                                                                            tAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGAc                                                           <  1:1081719/140‑1 (MQ=255)

                                                                            tAATGAGCGCGGTGCGCAAAGTGCTGTCTGCGCCGCAGCAAACCCATCTGAGTCGGGAGTAGGCGGTTTTTCGGTCAGTGGGCTGGGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGAc                                                           <  2:1271368/140‑1 (MQ=255)

                                                                                gAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCa                                                       <  2:10139755/140‑1 (MQ=255)

                                                                                  gcgcGGTGCGCAAACTGCTTTCAGCGCCGCAGCACACCCCACTGATTCTGGAGTCGTCCGATTGTCAGTCAGTTGGCTGTGGTACTGCCGGCCAAATTCCGACGGCGAGCGATCGGTGTTTTTACGAAACAGACGGCAgc                                                     <  2:9721295/140‑2 (MQ=255)

                                                                                    gcgGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAag                                                   >  1:3241551/1‑140 (MQ=255)

                                                                                    gcgGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAag                                                   >  2:5416629/1‑140 (MQ=255)

                                                                                    gcgGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAag                                                   >  2:1503015/1‑140 (MQ=255)

                                                                                    gcgGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAag                                                   >  1:1699028/1‑140 (MQ=255)

                                                                                    gcgGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAag                                                   >  1:7010008/1‑140 (MQ=255)

                                                                                     cgGTGCGCAACGTGCTTTCTGCGCCGCAGCTAACCCATCTTATGCTGGTGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTAGTGCCGGCGATTTGGCGACGGCGTGGGTGCGGTGTTTGTAGGGAACTGTCGGCAGatgg                                                  >  2:8195199/1‑137 (MQ=255)

                                                                                     cgGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAagt                                                  >  1:9616199/1‑140 (MQ=255)

                                                                                     cgGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAagt                                                  >  1:8491110/1‑140 (MQ=255)

                                                                                     cgGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAagt                                                  >  1:10274192/1‑140 (MQ=255)

                                                                                       gagcgcAAAGTGCTTTCTGCGCCGCAGCACACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAagtag                                                <  1:6351968/138‑1 (MQ=255)

                                                                                        tGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAcgtagt                                               >  2:8735755/1‑140 (MQ=255)

                                                                                        tGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAagtagt                                               >  1:7583236/1‑140 (MQ=255)

                                                                                           gcAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGc                                            <  2:4158322/140‑1 (MQ=255)

                                                                                            cAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCt                                           >  1:4091651/1‑140 (MQ=255)

                                                                                            cAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCt                                           <  2:3889864/140‑1 (MQ=255)

                                                                                                  gCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGAc                                     <  1:1522903/140‑1 (MQ=255)

                                                                                                  gCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGAc                                     <  1:8348376/140‑1 (MQ=255)

                                                                                                   cTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACa                                    <  1:4488841/140‑1 (MQ=255)

                                                                                                    tttCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACaa                                   <  2:106600/140‑1 (MQ=255)

                                                                                                           gccgccGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCGGcg                            >  2:7756336/1‑139 (MQ=255)

                                                                                                           gccgcAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCa                            >  2:1994251/1‑140 (MQ=255)

                                                                                                           gccgcAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCa                            >  1:5333870/1‑140 (MQ=255)

                                                                                                             cgcAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAgg                          >  1:11018476/1‑140 (MQ=255)

                                                                                                                  caAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTgc                     >  2:4013550/1‑140 (MQ=255)

                                                                                                                      cccATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCa                 <  1:6841330/140‑1 (MQ=255)

                                                                                                                      cccATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCa                 <  1:3540003/140‑1 (MQ=255)

                                                                                                                       ccATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACATACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCAc                >  2:2554620/1‑140 (MQ=255)

                                                                                                                       ccATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCAc                >  2:5906902/1‑140 (MQ=255)

                                                                                                                       ccATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCAc                >  2:7474183/1‑140 (MQ=255)

                                                                                                                         aTCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCActt              >  1:10098953/1‑140 (MQ=255)

                                                                                                                          tCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCActtc             >  1:3641175/1‑140 (MQ=255)

                                                                                                                          tCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCActtc             >  1:6174889/1‑140 (MQ=255)

                                                                                                                            ttATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTTCTGTCGACAAAGCCGCAGGCGTGCGCCActtctt           >  1:3372595/1‑140 (MQ=255)

                                                                                                                            ttATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCActtctt           <  1:6626106/140‑1 (MQ=255)

                                                                                                                              aTTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCtttt         <  1:2409124/140‑1 (MQ=255)

                                                                                                                                tCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTAc       <  1:5774300/140‑1 (MQ=255)

                                                                                                                                 cTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTAcc      >  1:2003995/1‑140 (MQ=255)

                                                                                                                                   ggAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCtt    <  2:7315908/140‑1 (MQ=255)

                                                                                                                                   ggAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCtt    <  2:758455/140‑1 (MQ=255)

                                                                                                                                   ggAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGGTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCtt    <  2:7941231/140‑1 (MQ=255)

                                                                                                                                    tagtagtCGGATTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATTTTCCGACGGCGAGCGTTCGGTTTTTTTACGATACATACGGCAGAAGTAGTTGCTGTCTACAAAGCCGCAGGCGTGCGCCACTTCTTTTACATTc   >  2:10373820/2‑140 (MQ=255)

                                                                                                                                    gAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTc   <  1:418893/140‑1 (MQ=255)

                                                                                                                                    gAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTc   >  1:672655/1‑140 (MQ=255)

                                                                                                                                    gAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTc   >  1:720934/1‑140 (MQ=255)

                                                                                                                                     agtagtCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCa  <  2:1809038/140‑1 (MQ=255)

GCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAATCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCA  >  NZ_CP025268/4149264‑4149536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`