BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,501,415	A→G	410 (TAA→TAG)	CXP41_RS23285 →	IS3‑like element IS2 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,501,415	0	A	G	99.6%	2400.0 / NA	753	410 (TAA→TAG)	CXP41_RS23285	IS3‑like element IS2 family transposase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (300/449); minor base T (0/3); total (300/453)
Fisher's exact test for biased strand distribution p-value = 2.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 753 total aligned reads displayed.

TGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGT  >  NZ_CP025268/4501289‑4501551

tGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCTCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATTTCTGGAAATATAGGGGCAAATCCAcc                                                                                                                             <  2:2576476/140‑1 (MQ=2)

tGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAcc                                                                                                                             >  2:5598245/1‑140 (MQ=11)

tGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGGTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAcc                                                                                                                             >  2:440400/1‑140 (MQ=11)

 gCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCt                                                                                                                            >  2:3672186/1‑140 (MQ=14)

     agGCGTTCGAGCATTATAACGAATGGNATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTtgtg                                                                                                                        <  1:6789204/140‑1 (MQ=21)

     agGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTtgtg                                                                                                                        <  2:8195612/140‑1 (MQ=21)

     agGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTtgtg                                                                                                                        <  1:4242603/140‑1 (MQ=21)

       gCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCt                                                                                                                      <  1:1668223/140‑1 (MQ=37)

       gCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCt                                                                                                                      >  1:250124/1‑140 (MQ=37)

       gCGTTCGAGCATGAGATCGAATGGCATCCGCAGAGTGCGCTGGGTTCTCGCTCGCCACGGGAAGATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACTGATGTCTGGAAATATAGGGGCAAATCCTCCTTGTGCt                                                                                                                      <  2:7053996/140‑1 (MQ=11)

        cGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGCTGTCTGGAAATATAGGGGCAAATCCACGTTGTGcgg                                                                                                                     >  2:9410109/1‑138 (MQ=255)

          ttCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGat                                                                                                                   >  2:1905910/1‑140 (MQ=255)

          ttCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGat                                                                                                                   <  2:10443044/140‑1 (MQ=255)

            cGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGatat                                                                                                                 <  1:7934317/140‑1 (MQ=255)

                    atcaCGAATGGCATCCGCATAGTGCGCTTGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACgg                                                                                                         <  2:9798072/137‑1 (MQ=255)

                    ataCCGAATGGCATCCGCATAGTGCGCTGGGTTCTCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATCGGGGCAAATCCACCTTGTGCTGATATGAATACgg                                                                                                         <  2:8800697/140‑1 (MQ=255)

                    ataACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACgg                                                                                                         <  1:1021458/140‑1 (MQ=255)

                    ataACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACgg                                                                                                         <  1:2074467/140‑1 (MQ=255)

                    ataACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACgg                                                                                                         <  2:2085520/140‑1 (MQ=255)

                    ataACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACgg                                                                                                         <  2:4249348/140‑1 (MQ=255)

                    ataACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACgg                                                                                                         <  1:5718229/140‑1 (MQ=255)

                    ataACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACgg                                                                                                         <  2:1570686/140‑1 (MQ=255)

                          aaTGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATAttt                                                                                                   >  1:10892491/1‑140 (MQ=255)

                             ggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTtat                                                                                                >  1:1808006/1‑140 (MQ=255)

                             ggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTtat                                                                                                >  2:1155586/1‑140 (MQ=255)

                             ggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTtat                                                                                                >  1:4466138/1‑140 (MQ=255)

                             ggCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTtat                                                                                                >  2:6119793/1‑140 (MQ=255)

                                atacGCATAGTGCGCTGGGTTCTCGCTCGACACGGGAATATCTGCGGCAGCTGGCTTGTAATGGGTTAAGTGATAACAGATGTGTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATCCGGATATTTTATAct                                                                                             <  2:6519018/137‑2 (MQ=17)

                                   cGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGaa                                                                                          <  2:1709605/140‑1 (MQ=255)

                                        aGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGCTATTTTATACAGACTACtt                                                                                     >  1:781161/1‑140 (MQ=255)

                                          tGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACtttt                                                                                   <  1:5647095/140‑1 (MQ=255)

                                          tGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTAGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACtttt                                                                                   >  2:2734157/1‑140 (MQ=255)

                                            cgcTGGGTTATCGCTCGCCACGGGAATCTCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCCAATCCCCCTTGTGCTGATATGAAGACGGATATGTTATACAGAATAGTTTTCa                                                                                 >  1:6168248/1‑140 (MQ=18)

                                            cgcTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCa                                                                                 <  1:1388216/140‑1 (MQ=255)

                                            cgcTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCa                                                                                 >  2:4874210/1‑140 (MQ=255)

                                            cgcTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCa                                                                                 <  2:6317150/140‑1 (MQ=255)

                                              cTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACgg                                                                                                         >  1:7014451/1‑114 (MQ=255)

                                               tGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTa                                                                              >  2:2862719/1‑140 (MQ=255)

                                               tGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTa                                                                              >  2:5748427/1‑140 (MQ=255)

                                                    tATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTTATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGc                                                                         <  1:10409087/140‑1 (MQ=255)

                                                      tctctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATATGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGa                                                                       <  2:7879958/137‑1 (MQ=255)

                                                      tcgctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGTTTAAGTTATAACAGATGTCTGGAAATATAGGGGCAAACCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGa                                                                       <  1:9418958/140‑1 (MQ=255)

                                                      tcgctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACATATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGa                                                                       <  1:10066346/140‑1 (MQ=255)

                                                      tcgctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATCGGGGCAAAACCACCTTGTGCTGATATGAAACCGGATATTTTATACAGAATAATTTTCAGAACAAGCGa                                                                       <  1:4792051/140‑1 (MQ=21)

                                                      tcgctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCTGTACAAGCGa                                                                       <  1:6666672/140‑1 (MQ=255)

                                                      tcgctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGa                                                                       <  2:2448607/140‑1 (MQ=255)

                                                      tcgctcgcCACGGGAATATCTGCGGCAGCGGGATTTTAATGGGTTAAGTGATAACAGATGTCTGGAATTTTAGGGGCAAATCCACCTTGTGCTGATATGAATACGTATATTTTATACAGAATACTTTTCAGTACAAGCGa                                                                       <  2:5884257/140‑1 (MQ=21)

                                                      tcgctcgcAACGGTAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACACATGTCTGTAAATATAGGGGAAAAGCCACCTTGTGCTGATATGAAGCCGGATATTTTATACAGAATACTTTTCAGTAAAAGCGa                                                                       <  2:3057846/140‑1 (MQ=21)

                                                           cgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTtgtg                                                                                                                        >  2:5097613/1‑86 (MQ=255)

                                                           cgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTa                                                                  >  2:5269835/1‑140 (MQ=255)

                                                             ccACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATTTAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATCTTTTATACCGAATACTTTTCAGTACAAGCGACTGTagg                                                                >  2:9041902/1‑138 (MQ=25)

                                                              cACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTa                                                               <  1:8086243/140‑1 (MQ=255)

                                                                 ggtaaTATCTGCGGCCGCGGGCTGGTACTGGTTTACGTGATAACTGATTTCTGGAAATATCGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGATTACTTTTCATTACAAGCGACTGTATTACAg                                                            <  1:3438363/137‑1 (MQ=255)

                                                                  gtaaTATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGt                                                           <  1:6382314/138‑1 (MQ=255)

                                                                  gtaaTATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGt                                                           <  2:9158224/138‑1 (MQ=255)

                                                                  ggAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTTATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCTACTGTATTACAGt                                                           <  1:9703547/140‑1 (MQ=255)

                                                                  ggAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGt                                                           <  2:1274677/140‑1 (MQ=255)

                                                                  ggAATATCTGCGGCAGCGGGCTTGAAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGt                                                           <  2:4741478/140‑1 (MQ=255)

                                                                   gAAGATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGAGGTCTGTAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAGTACTTTTCAGTACAAGCGACTGTATTACAGTc                                                          <  2:8486018/140‑1 (MQ=37)

                                                                     atatCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATCGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTg                                                        <  2:10885940/140‑1 (MQ=255)

                                                                     atatCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTg                                                        <  1:8371836/140‑1 (MQ=255)

                                                                      tatCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGc                                                       >  1:6550990/1‑140 (MQ=255)

                                                                        tCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGTAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCa                                                     <  1:7233478/140‑1 (MQ=255)

                                                                        tCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCa                                                     >  2:10661686/1‑140 (MQ=255)

                                                                        tCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCa                                                     >  1:8586666/1‑140 (MQ=255)

                                                                             ggCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCatatat                                                >  2:5455928/1‑140 (MQ=255)

                                                                             ggCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCatatat                                                >  2:6668414/1‑140 (MQ=255)

                                                                                  cGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTa                                           >  2:10193670/1‑140 (MQ=255)

                                                                                  cGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTa                                           >  1:9845321/1‑140 (MQ=255)

                                                                                  cGGGCTTGTAATGGGTTAAGTGATAAAAGATGTCTGGAAATATAGGGGCAATTCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCTGTACAAGCGACGGTATGACAGTCTGCCATATATGATTa                                           >  1:5163478/1‑140 (MQ=37)

                                                                                   gggCGTTTAATGGGTTTAGTGAAAACATTTGTGTGGAAATATAGGGGAAATACCAACTTGTGATTATATTAATCCGGTTATTTTATACATAATAATTTTCAGTAAAAGCTACTGTATTACAGTCTGCCATATATGATTAc                                          <  1:4064361/140‑1 (MQ=255)

                                                                                    ggCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTAcc                                         >  1:10694169/1‑140 (MQ=255)

                                                                                    ggCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCAGATATGATTAcc                                         >  1:11265146/1‑140 (MQ=255)

                                                                                           aaTGGGTTAAGTGATAACAGATGTCTGGAAATATCGGGGCAAATCCACATTGTGATGATATGAATCCGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATc                                  <  1:7666995/140‑1 (MQ=38)

                                                                                              gggTTAAGTTATAACAGATGTCTGGAAATATCGGGGAAAATCAACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCTGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAgg                               <  1:3733116/140‑1 (MQ=255)

                                                                                              gggTTAAGTGATAACAGCTGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTACAGTAAAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAgg                               <  1:8737941/140‑1 (MQ=255)

                                                                                               ggTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGga                              >  1:3067560/1‑140 (MQ=255)

                                                                                               ggTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGga                              >  1:5438788/1‑140 (MQ=255)

                                                                                                gTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGgaa                             >  2:3231007/1‑140 (MQ=255)

                                                                                                 ttAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGgaag                            >  1:500863/1‑140 (MQ=255)

                                                                                                 gtAAGTCATAACAGATGTCAGGAAATAAAGGGGCAAATCCACCTGGTGCTGAGATGAATACGGATATTTTATACAGAATACTGTTCAGTACAAGCGACTGTATTACAGGCTGCCATCTCTGAGTACCTGTGATCAGgacg                            >  2:6824276/2‑138 (MQ=255)

                                                                                                    aGTGATAACAGATGTCTGTAAATATAGGGGCAAATCCACCTTTTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGAACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAAt                         <  2:9624782/140‑1 (MQ=255)

                                                                                                    aGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATCTTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGTTTACCTGTGATCAGGAAGAAt                         >  1:8991892/1‑140 (MQ=255)

                                                                                                    aGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGTAt                         >  2:8907140/1‑140 (MQ=255)

                                                                                                    aGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAAt                         >  1:4623529/1‑140 (MQ=255)

                                                                                                    aGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAAt                         <  2:3473959/140‑1 (MQ=255)

                                                                                                       gATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAg                      <  1:11092911/140‑1 (MQ=255)

                                                                                                         tAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTg                    >  1:9246608/1‑140 (MQ=255)

                                                                                                         tAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTg                    >  2:3429931/1‑140 (MQ=255)

                                                                                                         tAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATANGATTACCTGTGATCAGGAAGAATAAGTg                    >  1:5952147/1‑140 (MQ=255)

                                                                                                          aaCAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTgg                   >  2:3812905/1‑140 (MQ=255)

                                                                                                           aCAGTTGTCTGGAAATATTGGGCCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACATAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGc                  <  1:283550/140‑1 (MQ=255)

                                                                                                             aGATTTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTg                <  2:4115020/140‑1 (MQ=255)

                                                                                                              gATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACTGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGa               >  2:3995941/1‑140 (MQ=255)

                                                                                                                tGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGa                                                                       <  2:7366143/82‑1 (MQ=255)

                                                                                                                tGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAgg             >  2:7600548/1‑140 (MQ=255)

                                                                                                                tGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAgg             >  1:324744/1‑140 (MQ=255)

                                                                                                                       aaaTATAGGGGCAAATCCACCTTGTGCTGATATTAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCaaa      <  1:1233576/140‑1 (MQ=255)

                                                                                                                       aaaTATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCaaa      <  1:7410989/140‑1 (MQ=255)

                                                                                                                       aaaTATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCaaa      <  2:4560033/140‑1 (MQ=255)

TGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAAGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGT  >  NZ_CP025268/4501289‑4501551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`