BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,608,154	G→A	noncoding (48/87 nt)	CXP41_RS23870 ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,608,154	0	G	A	98.6%	1431.2 / NA	435	noncoding (48/87 nt)	CXP41_RS23870	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base G (4/2); major base A (240/178); minor base T (2/4); total (247/188)
Fisher's exact test for biased strand distribution p-value = 4.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 436 total aligned reads displayed.

CCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCG  >  NZ_CP025268/4608019‑4608293

 ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCTCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                                                                        <  1:3717095/140‑1 (MQ=255)

 ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                                                                        <  1:839837/140‑1 (MQ=255)

 ccACGTCCGTAAGGACAATTACACCTGAAGCTAGCGAGTCTTCCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGACTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                                                                        <  2:4802977/140‑1 (MQ=37)

  cACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTa                                                                                                                                       <  2:6132189/140‑1 (MQ=255)

   aCGTCCGTTAGGACACTAACACCTGAAGCTAGCGCGTATACCAAGTCCGCCACCTTCTCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                                                      <  2:10454560/140‑1 (MQ=37)

   aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                                                      >  1:6302196/1‑140 (MQ=255)

   aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                                                      <  1:3932016/140‑1 (MQ=255)

   aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTGTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTat                                                                                                                                      >  1:10047519/1‑139 (MQ=255)

   aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTAGATATCGACTCGTTTGGTGCGAGGGGGGGGACTTGAACCACCACGTCCGTAAGAACACTaa                                                                                                                                      >  2:8621668/1‑140 (MQ=255)

     gtcctaaaGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAaca                                                                                                                                    <  1:10700088/134‑1 (MQ=255)

       ccGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                                                                                                  <  1:8807912/140‑1 (MQ=255)

          tAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAGAGTCAGCTTTCTTTTATTGATATCGCCTCGTGGGGTGCGAGGGGGGGGACTTGCACCACCACGACCCTAAGAACACAAACACCTGa                                                                                                                               >  2:4927179/1‑140 (MQ=21)

              gACACTAACACATGAAGCTTTGTCGTCTACAAATTCCGCCAACTTCTAACATTAATCTTAATTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCt                                                                                                                           <  1:2097078/140‑1 (MQ=21)

                cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAg                                                                                                                         >  1:9772894/1‑140 (MQ=255)

                 acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAATCTAgc                                                                                                                        >  2:8106929/1‑140 (MQ=255)

                 acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                                                                                                        >  1:10821465/1‑140 (MQ=255)

                 acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACACCCACGTCCGTAAGAACAATAACACCTGAAGCTAgc                                                                                                                        >  2:5165432/1‑140 (MQ=255)

                 acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCATCGTTTGGTGCGAGGGGGGGGACTAGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                                                                                                        >  2:848751/1‑140 (MQ=255)

                 acTAACACCTGAAGCTAGCGCGTCTACCAAGTCCGCCACCTTCGCACAGTCAGCTTCCTTTTTTTGATATCGCCGCGGTTGGTGCGAGGGGGGGGCCGTGTACCCCCACGTCCGGACGAACACTGACACCTGAAGCTAgc                                                                                                                        >  2:2292772/1‑140 (MQ=25)

                   tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcgc                                                                                                                      >  1:9621685/1‑140 (MQ=255)

                    aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg                                                                                                                     >  2:6641147/1‑140 (MQ=255)

                    aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg                                                                                                                     >  2:6537251/1‑140 (MQ=255)

                         cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACCCTAACACCTGAAGCTAGCGCGTCTAc                                                                                                                >  1:8716735/1‑140 (MQ=255)

                         cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTGTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAc                                                                                                                >  1:8618140/1‑140 (MQ=255)

                           gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGACCACTAACACCTGAAGCTAGCGCGGCTACCa                                                                                                              >  1:3628511/1‑140 (MQ=255)

                             aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATTTCGCCTCGTTTTGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAAAACTAACACCTGAAGCTAGATCGTCTACCAAt                                                                                                            >  2:7918506/1‑140 (MQ=25)

                             aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                                                                                            >  1:3230076/1‑140 (MQ=255)

                                 aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCAGATTACTTTTTTTGATATCGCGGCGTTTGGTGCGAGGGGGGGGACGTGAACCCCCACGTCCGTAAGAACACTATCACCTGTAGCTAGCGCGTCTTCCGATTCCg                                                                                                        >  1:1031978/1‑140 (MQ=21)

                                  gctcgTCTACAAATTCCGCCACCAAAGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                                                       <  1:7602642/137‑1 (MQ=21)

                                  gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                                                       <  1:2894523/140‑1 (MQ=255)

                                   cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                                                                                                      >  2:7558758/1‑140 (MQ=255)

                                   cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                                                                                                      >  2:1298631/1‑140 (MQ=255)

                                      gTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                                                                   >  1:7310445/1‑140 (MQ=255)

                                      gTCTACCAATTCCGCCACCTTCGCACAGTCAACTTACATTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                                                                   <  1:824877/140‑1 (MQ=255)

                                       tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                                                                                                  >  2:5110166/1‑140 (MQ=255)

                                           ccAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc                                                                                              <  1:11105195/140‑1 (MQ=255)

                                           ccAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc                                                                                              <  1:6640936/140‑1 (MQ=255)

                                            ctaTTCCGCCACCTTCCAACAGTCATGTTACTTTTTTTGATATCGACTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                             <  1:192034/138‑1 (MQ=18)

                                            cAATTCCGCCACCTTCTCACATTCATCTTACTTTTTTTGATATCGCCTATTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                             <  1:4626431/140‑1 (MQ=18)

                                            cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                             <  1:4112046/140‑1 (MQ=255)

                                            cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                             <  2:32224/140‑1 (MQ=255)

                                            cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                             <  2:9582507/140‑1 (MQ=255)

                                            cAATTCCGCCAACTTCTAACAGTCATCTTACTTTTTTTGATATCTCCTCGTTTGGTGTGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                             <  2:9771127/140‑1 (MQ=18)

                                                tCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt                                                                                         <  1:1656070/140‑1 (MQ=255)

                                                tCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt                                                                                         >  2:11128870/1‑140 (MQ=255)

                                                 ccGCCACGTTCTCTCATTCATCTTACTTTTTTTGATATAGCCTCTATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                                                        <  2:1951772/140‑1 (MQ=17)

                                                 ccGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                                                        <  1:643441/140‑1 (MQ=255)

                                                 ccGCCACCTTCGCACAGTCATCTTACNTTTTTTGATATCGCCTCGATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                                                        <  1:6793341/140‑1 (MQ=255)

                                                  cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc                                                                                       >  1:5093691/1‑140 (MQ=255)

                                                  cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc                                                                                       >  1:4866805/1‑140 (MQ=255)

                                                  cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc                                                                                       >  2:10153067/1‑140 (MQ=255)

                                                   gCCACGTTCGCACAGTCATCTTACATTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg                                                                                      <  1:10917388/140‑1 (MQ=255)

                                                    ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTACGAACACTAACACCTGAAGCTAGCGCGTCTACCACTTCCGCCACCTTCGCCCAGAGCGa                                                                                     >  2:4716241/1‑140 (MQ=255)

                                                    ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGa                                                                                     >  1:2255459/1‑140 (MQ=255)

                                                     cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                                                    <  1:3312755/140‑1 (MQ=255)

                                                             cacaGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCa                                                                            >  1:6821172/1‑140 (MQ=255)

                                                                 gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGAGGGAGGTATATCAACGt                                                                        >  1:6035158/1‑140 (MQ=18)

                                                                  tcttgttACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTGTAACCCCCCCGTCCGTAAGAACACAAACACATGAAGCTACGGCGTCACCCCATTCCGCCCCCTTCGCCCAGAGAGAGATATATCAACggg                                                                       >  2:11037379/6‑138 (MQ=16)

                                                                  tcttCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                                                                          <  2:8750616/134‑1 (MQ=255)

                                                                  tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                                                    >  2:5476560/1‑127 (MQ=255)

                                                                  tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCAACggg                                                                       >  2:1726677/1‑138 (MQ=255)

                                                                  tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTg                                                                       >  2:921273/1‑140 (MQ=255)

                                                                  tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTg                                                                       >  1:2493067/1‑140 (MQ=255)

                                                                  tCATCTTACTGTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTCGCGCGTCTACCAATTCCGCCACCTTCGCCCAGGGCGAGCAATATCAACGTg                                                                       >  1:786193/1‑140 (MQ=255)

                                                                     tCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGtt                                                                    >  2:10781692/1‑140 (MQ=255)

                                                                          cTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCAAGAGAGAGCACTATAAAAGTGGTTTTTgg                                                               >  1:5379640/1‑140 (MQ=18)

                                                                            ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCAAGTGCGAGCAATATCAACGTGGTTTTTGGTg                                                             >  2:9065992/1‑140 (MQ=255)

                                                                            ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGAGAGAGCACTATCAACGAGGTGTTTGCTg                                                             >  2:5949286/1‑140 (MQ=17)

                                                                              ttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGAGCCAGCTCTAACAAAGAAGTTTTAGGAGCg                                                           >  2:4099831/1‑140 (MQ=14)

                                                                                 tgctatCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACGAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGGGGTTTTTGGTGCGAgg                                                        <  2:2517229/137‑1 (MQ=255)

                                                                                    tctCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGCACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg                                                     <  1:5782646/138‑1 (MQ=255)

                                                                                    tatCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg                                                     <  2:10301373/140‑1 (MQ=255)

                                                                                    tatCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg                                                     <  2:9295916/140‑1 (MQ=255)

                                                                                    tatCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATAACAACGTGGTTTTTGGTGCGAggggg                                                     <  1:9491811/140‑1 (MQ=255)

                                                                                       cgacTCGTTTGGTGCGAGGGGGGGGACTTGAACACCCAAGTCCGTAAGAACACTAACACCTGAAGCTAGCTCGTCTACCAATTCCGCCACCTTCGCCCACTGAGAGAAATATCAACGATGTTTTAGGTGCGAggggggtc                                                  >  2:11290497/4‑138 (MQ=17)

                                                                                            cGTTTGGAGCGAGGGGGGGGACTTGAACCCCCACGTCCTTAAGAACACAAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTg                                             <  1:1227594/140‑1 (MQ=255)

                                                                                                tGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGAGGTGTTTGGTGCGAGGGGGGGGACTTGaaac                                         >  2:6803344/1‑138 (MQ=255)

                                                                                                  gTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATAACAACGTGGTTTTTGGTGCGAGGGGGGGGCCTGGAAcccc                                       >  1:9347508/1‑140 (MQ=255)

                                                                                                    gCGTGGGGGGGGACTAGAACCCCCACGTCCGACAGAACCCCCCCACCGGAACCTCCCGCGTCTTCCAAGTCCGACACCCTCGCCCAGTGCGAGCAATATCTACGTGCTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCa                                     <  2:2903399/140‑1 (MQ=255)

                                                                                                      gAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACg                                   <  1:5491661/140‑1 (MQ=255)

                                                                                                        ggggggggNCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCNACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTc                                 >  1:6002949/1‑140 (MQ=255)

                                                                                                        ggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTc                                 <  1:1342126/140‑1 (MQ=255)

                                                                                                             gggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTaa                            >  1:9822732/1‑140 (MQ=255)

                                                                                                                  ttGACCCCCCACGTCGGTAAGAACACAAACACCTGAAGATCTCGCGTCTACAAATTCCGACACCTTCGCCCAGTCCGAGCAATATCAACTTGGTATTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGaca                       <  1:1504492/140‑1 (MQ=25)

                                                                                                                    gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                     >  2:10086955/1‑140 (MQ=255)

                                                                                                                    gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                     >  2:10931869/1‑140 (MQ=255)

                                                                                                                    gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                     >  2:3815771/1‑140 (MQ=255)

                                                                                                                    gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGAAAGGACACt                     >  1:2655906/1‑140 (MQ=255)

                                                                                                                     aaCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTa                    >  2:4238176/1‑140 (MQ=255)

                                                                                                                     aaCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCACTGCGAGCAATATCAACGAGGTTTTAGGTGCGAGGGGGGGGAATTGAACACCCACGTCCGGAAGGAAACTa                    >  2:3406326/1‑140 (MQ=25)

                                                                                                                          ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc               >  2:4619138/1‑140 (MQ=255)

                                                                                                                          ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc               <  1:10499506/140‑1 (MQ=255)

                                                                                                                          ccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc               >  2:7389414/1‑140 (MQ=255)

                                                                                                                           cACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCt              >  2:3925021/1‑140 (MQ=255)

                                                                                                                            aCGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCAGTAAGGACACTAACAACTg             >  2:7719464/1‑140 (MQ=255)

                                                                                                                            aCGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTg             <  1:9125849/140‑1 (MQ=255)

                                                                                                                            aCGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTg             >  1:8040927/1‑140 (MQ=255)

                                                                                                                               tCCGTACGAACACTAACACCTGAAGCTAGCGCGTCTCCCCATTACTCCACCTTCGCCCCGTGCGAGCAATATCAACGGGGATGTGGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTCAGGTCACTCACACGTGAAg          >  2:5699638/1‑140 (MQ=21)

                                                                                                                                   tAAGCACCCTAACACCAGAACCTAGCGCGTCTACCAATTCCGCCCCCTTCGCCCAGTGCTAGCAATCTCACCGTGTTTTTTGTTGCGAGGGGGGGGACTTGAACCCCCACGTCCGAAAGGACACTAACACCTGAAGCTAg      <  2:3018388/140‑1 (MQ=21)

                                                                                                                                   tAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg      >  1:6738543/1‑140 (MQ=255)

CCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCG  >  NZ_CP025268/4608019‑4608293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`