BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	442,101	T→C	197 (TAA→TAG)	yajL ←	protein deglycase YajL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	442,101	0	T	C	99.8%	1567.5 / NA	450	197 (TAA→TAG)	yajL	protein deglycase YajL
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (259/190); minor base A (1/0); total (260/190)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 455 total aligned reads displayed.

TGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGC  >  NZ_CP025268/441964‑442236

    aGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTACTGTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCg                                                                                                                                   >  1:450731/1‑140 (MQ=255)

    aGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGACGCATCCGACACTACTCg                                                                                                                                   >  2:9081472/1‑140 (MQ=255)

      cgcgGGGTGATGCGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACACTGTGAAGGTATATCGCCCGTAACTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACTTGTGCCGCATCCGACACTACTCGta                                                                                                                                 <  2:5361789/140‑1 (MQ=255)

      cgcgGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGta                                                                                                                                 >  1:1962765/1‑140 (MQ=255)

       gcgGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGTCTTTAACAATGTGAAGGTATATCGCCCGTAAGTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaa                                                                                                                                >  2:10378726/1‑140 (MQ=255)

       gcgGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaa                                                                                                                                >  2:11323510/1‑140 (MQ=255)

       gcgGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaa                                                                                                                                >  1:782802/1‑140 (MQ=255)

         ggggTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGAATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaata                                                                                                                              <  2:7616604/140‑1 (MQ=255)

          gggTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGACGGTATATCGCCCGTAATTTGTGGTTTTTTCGTCGCATCTGGTCAGATGCGACGTGTGCCGCATCCGACACTACTCGtaataa                                                                                                                             >  1:5869373/1‑140 (MQ=255)

          gggTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaataa                                                                                                                             >  2:4071880/1‑140 (MQ=255)

          gggTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaataa                                                                                                                             >  2:9696574/1‑140 (MQ=255)

          gggTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaataa                                                                                                                             <  2:2037069/140‑1 (MQ=255)

          gggTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGGAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaataa                                                                                                                             >  1:9218061/1‑140 (MQ=255)

          gggTGATGAGCCGTCTGCAGGCGCTCAATCTGCGCGTGCAGGGCTTTTACAATGTGAAGGTATATCGCCCGTAATTTGTGGTGTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaataa                                                                                                                             >  1:2107020/1‑140 (MQ=255)

                     cGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATccc                                                                                                                  >  1:5590653/1‑140 (MQ=255)

                     cGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATccc                                                                                                                  >  1:62926/1‑140 (MQ=255)

                       tCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTg                                                                                                                >  2:6815044/1‑140 (MQ=255)

                       tCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTg                                                                                                                >  1:4947754/1‑140 (MQ=255)

                       tCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTg                                                                                                                >  1:652364/1‑140 (MQ=255)

                       tCTGCAGGCGCTCAATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATATGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAAGTATAATTCACTg                                                                                                                >  2:7351470/1‑140 (MQ=255)

                         tGCAGGCGCTCTATCTGCGCGAGCAGGGCTGTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTgcc                                                                                                              >  2:5889523/1‑140 (MQ=255)

                          gCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATCTCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGGCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTgccg                                                                                                             <  1:1078358/140‑1 (MQ=255)

                          gCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTgccg                                                                                                             <  1:10346412/140‑1 (MQ=255)

                            aGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTgccgcc                                                                                                           <  1:3455212/140‑1 (MQ=255)

                              gcgcTCTAACTGCGCGAGCAGGGCTTTAACACTGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGAAGCGACGTTTGCCGCATCCGACACTACTCGTAATACTTATAAATCCCTGCCGCCAt                                                                                                         <  1:5200735/140‑1 (MQ=255)

                                 ctctATCTGCGCGAGCAGTGCTTTAACCATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCAc                                                                                                      <  2:2990568/140‑1 (MQ=255)

                                 ctctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCGCGTAATTTGTGGTTGTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACCCTCCTCGTCATAATTATACATCCCTGCCGCCATCAc                                                                                                      >  2:6146631/1‑140 (MQ=255)

                                 ctctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCAc                                                                                                      >  1:2867702/1‑140 (MQ=255)

                                 ctctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCAc                                                                                                      >  2:5035857/1‑140 (MQ=255)

                                 ctctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCAc                                                                                                      >  2:8041197/1‑140 (MQ=255)

                                   ctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCa                                                                                                    >  2:8324859/1‑140 (MQ=255)

                                   ctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCa                                                                                                    <  2:2754339/140‑1 (MQ=255)

                                     aTCTGCGCGAGCAGGGCTTTAACACTGTGATGGTATCTCGCCCGTAATGTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGt                                                                                                  <  2:10677284/140‑1 (MQ=255)

                                       cTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTtg                                                                                                <  2:2484247/140‑1 (MQ=255)

                                          cgcgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGa                                                                                             >  2:9292898/1‑140 (MQ=255)

                                          cgcgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGa                                                                                             >  2:6334839/1‑140 (MQ=255)

                                            cgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCTCCAGTTGTGATg                                                                                           >  1:9160193/1‑140 (MQ=255)

                                            cgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATg                                                                                           >  2:10984545/1‑140 (MQ=255)

                                             gAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGc                                                                                          <  1:1592157/140‑1 (MQ=255)

                                              aGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaat                                                                                                                               <  2:6953566/102‑1 (MQ=255)

                                                        tAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCCTCACCAGTTGTGATGCCACTTCATggg                                                                               >  2:5492912/1‑140 (MQ=255)

                                                         aaCAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGc                                                                              >  1:3078130/1‑140 (MQ=255)

                                                         aaCAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACAAGTTGTGATGCCACTTCATGGGc                                                                              >  1:2626306/1‑140 (MQ=255)

                                                             aTGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCtttt                                                                          >  2:10552446/1‑140 (MQ=255)

                                                             aTGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCtttt                                                                          >  1:10438419/1‑140 (MQ=255)

                                                             aTGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCtgtt                                                                          >  1:10829175/1‑140 (MQ=255)

                                                              tgtgAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCCTGGGCttttt                                                                         <  1:9510775/140‑1 (MQ=255)

                                                                 gAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCAc                                                                      <  1:4018679/140‑1 (MQ=255)

                                                                  aaGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGCCGTTTGCCGCATCCGACACTACGCGTAATCTTTATAAATCACTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACg                                                                     >  2:6610034/1‑140 (MQ=255)

                                                                   aGGTATATCGCCAGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGc                                                                    >  1:7024394/1‑140 (MQ=255)

                                                                    ggTATATCGCCCGTAATTGGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTCCTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGcc                                                                   <  1:10701165/140‑1 (MQ=255)

                                                                        tatCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCccaac                                                               >  2:3354368/1‑140 (MQ=255)

                                                                        tatCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCccaac                                                               >  1:6454538/1‑140 (MQ=255)

                                                                        tatCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCccaac                                                               >  1:1336134/1‑140 (MQ=255)

                                                                        tatCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAAACCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCccaac                                                               >  2:1356954/1‑140 (MQ=255)

                                                                          tCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCTCGCccaacca                                                             >  2:7191875/1‑140 (MQ=255)

                                                                          tCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCccaacca                                                             >  1:6615652/1‑140 (MQ=255)

                                                                           cGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCccaaccaa                                                            <  1:837830/140‑1 (MQ=255)

                                                                            gCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCCTGGGCTTTTTCACGCCcaaccaac                                                           >  2:880719/1‑140 (MQ=255)

                                                                            gCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCcaaccaac                                                           >  1:3479882/1‑140 (MQ=255)

                                                                            gCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCcaaccaac                                                           <  1:9992670/140‑1 (MQ=255)

                                                                            gCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCC‑CATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACa                                                          >  2:7081354/1‑140 (MQ=255)

                                                                               cGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCAc                                                                      >  2:5734579/1‑126 (MQ=255)

                                                                               cGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGGAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCCCTTCATGGGCTTTTTCACGCCCAACCAACAgg                                                        >  2:9434263/1‑140 (MQ=255)

                                                                                gTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGt                                                       <  2:2159560/140‑1 (MQ=255)

                                                                                gTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGt                                                       <  1:773318/140‑1 (MQ=255)

                                                                                    tttGTGGTTTTTACGACGCATCGGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAAGAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGAt                                                   <  2:7848918/140‑1 (MQ=255)

                                                                                          gTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAAtttt                                             <  1:3119374/140‑1 (MQ=255)

                                                                                                 cGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAACTCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCa                                      <  1:9106903/140‑1 (MQ=255)

                                                                                                 cGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCa                                      <  2:4269163/140‑1 (MQ=255)

                                                                                                 cGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCa                                      <  2:5426538/140‑1 (MQ=255)

                                                                                                 cGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCa                                      <  1:867324/140‑1 (MQ=255)

                                                                                                     gCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACCCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGt                                  >  1:8886142/1‑140 (MQ=255)

                                                                                                      catgttgTCAGTTTCGACGTTTGCCGCTTCCGACACTACTCTTAATAATTATAAATTCCTGCCGCCATCACAAGTTGTGATGACACTTCAGGTGCTTTTTCTCTCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTc                                 <  2:7562070/134‑1 (MQ=255)

                                                                                                      cATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCACCCGGTCGATAATTTTCAGACCAAAGTc                                 <  2:4797180/140‑1 (MQ=255)

                                                                                                      cATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTc                                 <  1:975068/140‑1 (MQ=255)

                                                                                                       aTCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACAAACAGGTCGATAATTTTCAGACCAAAGTCg                                >  1:6919179/1‑140 (MQ=255)

                                                                                                        tCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGa                               <  1:1660748/140‑1 (MQ=255)

                                                                                                        tCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGa                               <  1:11074596/140‑1 (MQ=255)

                                                                                                            gTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGc                           >  2:3474437/1‑140 (MQ=255)

                                                                                                                  tGCGTCGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTGGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc                     <  1:4781242/140‑1 (MQ=255)

                                                                                                                  tGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATACATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc                     <  1:7223047/140‑1 (MQ=255)

                                                                                                                  tGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATACATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc                     <  2:4527791/140‑1 (MQ=255)

                                                                                                                  tGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc                     <  1:6011877/140‑1 (MQ=255)

                                                                                                                  tGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc                     <  2:3791718/140‑1 (MQ=255)

                                                                                                                  tGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc                     <  1:9630177/140‑1 (MQ=255)

                                                                                                                    cGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCg                   >  1:5098736/1‑140 (MQ=255)

                                                                                                                    cGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCg                   >  1:2434045/1‑140 (MQ=255)

                                                                                                                     gACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCgg                  <  1:7631085/140‑1 (MQ=255)

                                                                                                                       cGTTTGCCGCATCCGACACTACTCGTAATAATGATAAATCCCTGCCGCCATCCCCCGTTGTGATGCCACTTCATGGGCTATTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAACGTCGATAGCTGTACCCGGcc                <  1:807470/140‑1 (MQ=255)

                                                                                                                           tGCCGCATCCGACACTACTCGTAATAATTATACATCCCTGCCGCCATCACCAGTTGTGATGCCACTGCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCctg            >  2:178243/1‑140 (MQ=255)

                                                                                                                            gCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCctgg           <  1:3798057/140‑1 (MQ=255)

                                                                                                                            gCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCctgg           <  1:1454221/140‑1 (MQ=255)

                                                                                                                               gCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCctggctg        <  1:8260700/140‑1 (MQ=255)

                                                                                                                                 aTCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGt      >  1:6183425/1‑140 (MQ=255)

                                                                                                                                 aTCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCAACAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGt      >  2:7757710/1‑140 (MQ=255)

                                                                                                                                  tCCGACACTACTCGTAATAATTATAAATCCCTGCCTCCATCACCAGTTGTGATGCGACTTCATGGGCTTTTTCACGCCCAACCAACTGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGATGGTc     >  2:8454982/1‑140 (MQ=255)

                                                                                                                                   ccGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAACGTCGATAGCTGTACCCGGCCCCTGGCTGGTCa    <  1:4160658/140‑1 (MQ=255)

                                                                                                                                   ccGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCa    <  2:1628480/140‑1 (MQ=255)

                                                                                                                                     gACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGACCCCTGGCTGGTCAGc  <  1:3613102/140‑1 (MQ=255)

TGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGC  >  NZ_CP025268/441964‑442236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`