BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	356,677	2 bp→CC	intergenic (+172/+164)	codA → / ← cynR	cytosine/isoguanine deaminase/transcriptional regulator CynR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	356,677	0	T	C	96.3%	1546.5 / ‑4.2	545	intergenic (+172/+165)	codA/cynR	cytosine/isoguanine deaminase/transcriptional regulator CynR
Reads supporting (aligned to +/- strand): ref base T (3/0); major base C (191/328); minor base A (16/4); total (213/332)
Fisher's exact test for biased strand distribution p-value = 1.88e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NZ_CP025268	356,678	0	A	C	96.7%	1581.7 / 4.8	545	intergenic (+173/+164)	codA/cynR	cytosine/isoguanine deaminase/transcriptional regulator CynR
Reads supporting (aligned to +/- strand): ref base A (18/0); new base C (195/332); total (213/332)
Fisher's exact test for biased strand distribution p-value = 2.87e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 545 total aligned reads displayed.

ATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAG  >  NZ_CP025268/356540‑356811

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aTGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTACCGCCAACAACCGCGCATTAAt                                                                                                                                      >  1:7637650/1‑140 (MQ=255)

        tGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGcc                                                                                                                              <  1:4394477/140‑1 (MQ=255)

                        ccTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCt                                                                                                              >  1:1234062/1‑140 (MQ=255)

                         cTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGCTTCATTCCCGCCAACAACCGCGCATTGCTCCAACGCCATGGGCAACAATGCCtt                                                                                                             >  2:10191888/1‑140 (MQ=11)

                         cTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGAGCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCATCCAACGCCATGTGCAAAAATGCCtt                                                                                                             >  1:1128303/1‑140 (MQ=255)

                                 agCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTCATCCAACGCCATGTGCAAAAATGCCTTCGcggcgg                                                                                                     >  2:4021614/1‑140 (MQ=12)

                                 agCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCTGCgg                                                                                                     >  1:6586424/1‑140 (MQ=11)

                                 agCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCgg                                                                                                     <  2:6447596/140‑1 (MQ=255)

                                  gCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCggcggc                                                                                                    >  1:6515216/1‑140 (MQ=17)

                                  gCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGACAACACCCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGc                                                                                                    <  1:7065225/140‑1 (MQ=11)

                                   cACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCAGTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCt                                                                                                   <  2:3937924/140‑1 (MQ=11)

                                      tGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTc                                                                                                >  1:6180632/1‑140 (MQ=255)

                                      tGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCATCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTc                                                                                                >  1:4750287/1‑140 (MQ=255)

                                      tGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGACGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCTGCGGCTGTc                                                                                                >  1:6015038/1‑140 (MQ=11)

                                       gAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCt                                                                                               <  2:3059251/140‑1 (MQ=255)

                                        aaCTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTg                                                                                              >  2:7942711/1‑140 (MQ=255)

                                         aCTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGc                                                                                             <  2:9048688/140‑1 (MQ=255)

                                         aCTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCAGTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGc                                                                                             <  2:344919/140‑1 (MQ=11)

                                         aCTCGGAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGc                                                                                             <  2:2398062/140‑1 (MQ=11)

                                          cTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGcc                                                                                            <  1:7942711/140‑1 (MQ=255)

                                          cTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGcc                                                                                            <  1:1863251/140‑1 (MQ=255)

                                             gTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCAACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGc                                                                                         >  2:8939100/1‑140 (MQ=11)

                                              tAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCt                                                                                        <  1:3310732/140‑1 (MQ=255)

                                              tAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCt                                                                                        <  1:1099554/140‑1 (MQ=255)

                                              tAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGAGTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCt                                                                                        <  2:5181608/140‑1 (MQ=11)

                                               aGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTg                                                                                       <  1:6255793/140‑1 (MQ=255)

                                                     gATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGttt                                                                                 >  1:2706048/1‑140 (MQ=255)

                                                     gATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCAGGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTCTAGttg                                                                                 >  2:6298513/1‑139 (MQ=14)

                                                      aTAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTa                                                                                >  1:5454494/1‑140 (MQ=255)

                                                           cGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTGTAGTTTATGCCg                                                                           >  1:6101219/1‑140 (MQ=14)

                                                           cGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCg                                                                           >  1:6977890/1‑140 (MQ=255)

                                                           cGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCg                                                                           >  1:4019101/1‑140 (MQ=255)

                                                             tAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGa                                                                         >  1:9749941/1‑140 (MQ=255)

                                                               gcgcATCCGGCACTTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCAGTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAACATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATg                                                                       <  1:8326769/140‑1 (MQ=17)

                                                               gcgcATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATg                                                                       <  1:2329990/140‑1 (MQ=255)

                                                                cgcATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATgc                                                                      >  1:4234944/1‑140 (MQ=255)

                                                                 gcATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATgcg                                                                     <  1:9244089/140‑1 (MQ=255)

                                                                 gcATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATgcg                                                                     <  2:6977890/140‑1 (MQ=255)

                                                                  cATGAGGCAATTCCAGCGGCGGATCTGTGTCTGCGGCTACCGTGCTTCATTCCCGCCATCAAACGCGCAGCCCCCCCACGCCATGTGCTAAAATGCCTTCGCTTCGGCTGTAAGGCCCCCGTAGTTTATGCCGGATgcgg                                                                    <  2:9418769/140‑1 (MQ=255)

                                                                   aTCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACAGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATgcggc                                                                   >  1:2170757/1‑140 (MQ=17)

                                                                      aGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCCACACCCGCGCAGTCCTCCTACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTg                                                                <  2:5826608/140‑1 (MQ=18)

                                                                      aGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTg                                                                <  1:10345435/140‑1 (MQ=255)

                                                                      aGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTg                                                                <  1:1951211/140‑1 (MQ=255)

                                                                       ggCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACCCCATGTGCAAAACTGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGATTATGCCGGATGCGGCGTGa                                                               <  1:5664657/140‑1 (MQ=17)

                                                                       ggCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCAGTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGa                                                               <  2:5229309/140‑1 (MQ=18)

                                                                           aTTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCCACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGc                                                           <  1:5385644/140‑1 (MQ=18)

                                                                           aTTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGc                                                           <  2:1074023/140‑1 (MQ=255)

                                                                           aTTCCAGCCGCTGATCTGTCTCAGCGGCTACCGTGAGTCATTCCCGCCAACAACCGCGCAGTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGc                                                           <  1:3819093/140‑1 (MQ=18)

                                                                              ccAGCCGCTGATCTGTGTCAGCGGCTACCGTGAGTCAGTCCCGCCAACAACCGCGCAGTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCtt                                                        <  2:10911920/140‑1 (MQ=18)

                                                                                aGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACACCCGCGCAGTCCTCCCCCGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTGAt                                                      <  2:7475986/140‑1 (MQ=18)

                                                                                aGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTAt                                                      <  1:1375141/140‑1 (MQ=255)

                                                                                aGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTAt                                                      <  1:10732925/140‑1 (MQ=255)

                                                                                 gCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATc                                                     <  2:3657751/140‑1 (MQ=255)

                                                                                     cTGATCTGTGTCAGCGGCTACCGTGCTTCATGCCCGCCAACAACCGCGCAGTCCTCCAACGCCATGTGCAAAAATGCCTGCGCAGCGGCTGTCAGCCCGCGGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                 <  2:5577107/140‑1 (MQ=21)

                                                                                     cTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAACAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                 <  2:4710768/140‑1 (MQ=39)

                                                                                     cTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                 <  1:2281162/140‑1 (MQ=255)

                                                                                     cTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                 <  1:4108370/140‑1 (MQ=255)

                                                                                     cTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                 <  1:6698029/140‑1 (MQ=255)

                                                                                     cTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                 <  1:6861559/140‑1 (MQ=255)

                                                                                     cTGAGCTGTGTCAGCTGCTACCGTGAGTCATTCCCGCCAACAACCGCGCATTCCTCCCACGCCATGTGCAAAAATGCCTTCGCTGCGGGTGTCTGCCAGCGGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                 <  1:7387254/140‑1 (MQ=18)

                                                                                       gATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCt                                               <  2:8311315/140‑1 (MQ=255)

                                                                                       gATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCt                                               <  1:6360229/140‑1 (MQ=255)

                                                                                       gATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCt                                               <  1:2584649/140‑1 (MQ=255)

                                                                                       gATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCAGTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCt                                               <  1:49342/140‑1 (MQ=21)

                                                                                            gtgtCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTa                                          <  1:743382/140‑1 (MQ=255)

                                                                                            gtgtCAGCGGCTACCGAGATTCAGTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTa                                          <  2:9536613/140‑1 (MQ=21)

                                                                                             tgtCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTag                                         >  1:8869501/1‑140 (MQ=255)

                                                                                             tgtCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTag                                         >  1:1004133/1‑140 (MQ=255)

                                                                                              gtCAGCGGCTACCGTGAGTCATTCCCGCCCACACCCGCGCATTCCTCCCACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTaga                                        <  1:3596006/140‑1 (MQ=21)

                                                                                                 aGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCa                                     <  1:9953737/140‑1 (MQ=255)

                                                                                                 aGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCAGTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCa                                     <  1:3107563/140‑1 (MQ=25)

                                                                                                 aGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCAGTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCa                                     <  2:1046248/140‑1 (MQ=25)

                                                                                                  gCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGTATGCGGCGTGAACGCCTTATCGGGCGTCCGTAGAGCAc                                    >  1:4507118/1‑140 (MQ=21)

                                                                                                  gCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGCGAGCAc                                    >  2:2575554/1‑140 (MQ=21)

                                                                                                   cGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACt                                   >  1:8978437/1‑140 (MQ=255)

                                                                                                     gctACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGa                                 <  2:8496165/138‑1 (MQ=255)

                                                                                                     gctACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGa                                 <  2:1373355/138‑1 (MQ=255)

                                                                                                     gctACCGTGAGTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGa                                 <  1:5082698/138‑1 (MQ=25)

                                                                                                       tACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGGGATCTCCTTCTCCGGCCTACGTAGAGCTCTgtaa                               >  2:5956294/1‑137 (MQ=18)

                                                                                                         ccGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATcc                                                    >  2:6153407/1‑117 (MQ=255)

                                                                                                          cGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGACTTATCCGGCCTACGTAGAGCACTGAACTCg                            <  2:9641861/140‑1 (MQ=255)

                                                                                                          cGTGATTCATTCCCGCCAACAACCGCGCATTACTCAAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCg                            <  2:1637686/140‑1 (MQ=255)

                                                                                                            tGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTa                          <  1:9698519/140‑1 (MQ=255)

                                                                                                            tGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTa                          <  2:7255445/140‑1 (MQ=255)

                                                                                                            tGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTa                          >  2:920700/1‑140 (MQ=255)

                                                                                                            tGATTCAGTCCCGCCAACAACCGCGCATACCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTa                          <  2:6528708/140‑1 (MQ=37)

                                                                                                               ttCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGc                       <  1:9359430/140‑1 (MQ=255)

                                                                                                               ttCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGc                       <  2:4302264/140‑1 (MQ=255)

                                                                                                               ttCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGc                       <  1:1609687/140‑1 (MQ=255)

                                                                                                               ttCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGc                       <  2:1992351/140‑1 (MQ=255)

                                                                                                                  agtcccGACAACAACCGAGCAGTACTCAAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTg                    <  2:6915298/136‑1 (MQ=21)

                                                                                                                    tcccGCCAACACCCGCGCATTCCACCCACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGAt                  <  1:8776462/138‑1 (MQ=25)

                                                                                                                    tcccGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGAt                  <  2:2809647/138‑1 (MQ=255)

                                                                                                                        gCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGc              >  1:8608134/1‑140 (MQ=255)

                                                                                                                           cacaacCGCGCAGTCCTCCCACGCCATGTGCAACAATGCCTTCGCAGCGGCTGTCTGCCAGCGGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTa           <  2:4928456/139‑1 (MQ=21)

                                                                                                                            accacCGCGCATTCCTCCAACGCCATGTGCAAAAATCCCTTCTCAGCGGCTGTCAGCCTTCTTTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAg          <  2:537944/137‑1 (MQ=18)

                                                                                                                            acaacCGCGCAGTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCAGGCAGCTGTAGTTGTTGCCGGTTGCGTCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAg          <  2:11115713/140‑1 (MQ=21)

                                                                                                                                ccGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACTCCTTATCCGCCCTACGTAGAGCACTGAAATCGTATGACTGATAAACGTAGAGCa      >  1:10937309/1‑140 (MQ=25)

                                                                                                                                ccGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCa      <  1:550167/140‑1 (MQ=255)

                                                                                                                                    gcATTCCTCCCACGCCATGTGCACAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAg  <  2:334335/140‑1 (MQ=255)

||

ATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAG  >  NZ_CP025268/356540‑356811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`